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Biomedical subjects

L Fridhandler

Publications and source records attributed to L Fridhandler.

16 recordsLinked to original sources
PubMed1979

Value of pancreatic-type isoamylase assay as an index of pancreatic insufficiency.

The study here reported was undertaken to assess the value of assay of the specific isoamylase form arising from the pancreas (P-type) as an index of pancreatic exocrine insufficiency. Measurements were made of serum total amylase activity, serum P-type isoamylase activity, and the amount of P-type isoamylase relative to creatinine (Upam/Ucr) in the urine in a series of patients with clinically suspected chronic pancreatitis and pancreatic insufficiency as determined from the pancreatic secretory response to secretin stimulation. Abnormally low values for P-type isoamylase in the serum and urine of these patients were infrequent. Conversely, values within the normal ranges for serum P-type isoamylase and Upam/Ucr were common. It is concluded that while subnormal values for P-type isoamylase in the serum and urine may be viewed as supportive evidence for pancreatic insufficiency, failure to find such values does not exclude this condition.

Creatinine↗
PubMed1978

Macroamylasemia.

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Amylases↗
PubMed1977

Tumor-associated hyperamylasemia.

Studies were made in eight patients with hyperamylasemia associated with cancer of the lung, pancreas or colon. Isoamylase analysis, conducted in seven of the eight patients revealed that the dominant isoamylase component was S-type in three and P-type in three; in one patient both isoamylases were elevated about equally. Examination of the serum or urine of the same seven patients for subcomponent isoamylase disclosed the presence in two of the seven of an unusual isoamylase designated "Y-type". This subcomponent isoamylase was also found in some samples of human milk, but not in normal persons or in patients with a variety of disorders other than cancer. The significance of hyperamylasemia occurring in association with cancers, the factors responsible for the variability in isoamylase pattern in those with hyperamylasemia, and the relationship, if any, of the "Y" isoamylase form to cellular growth and replication are all presently obscure and remain to be elucidated.

Amylases↗
PubMed1977

Isoamylase analysis.

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Glycoside Hydrolases↗
PubMed1977

Assay of amylase and isoamylase activities in serum and urine. Modifications in methods and range of normal values.

Certain modifications are described in assay methods previously reported from this laboratory for total amylase and isoamylase activities. These modifications provided more consistent results and achieve superior separation of pancreatic and salivary type (P- and S-type) isoamylases. The estimated range of values in the serum and urine of normal subjects using the modified assay procedures is presented for total amylase as well as for P- and S-type isoamylases.

Adult↗
PubMed1976

Unusual isomaylase in cancer-associated hyperamylasemia.

Hyperamylasemia and hyperamylasuria were found in two patients with carcinoma of the pancreas and in two other patients with carcinoma of the lung. Detailed isoamylase analyses were conducted on the serum amylase of three and the urine amylase of all four of these patients, using a modified chromatographic procedure. The studies demonstrated the existence, in one of the lung cancer patients and in one of the patients with pancreatic cancer, of an unusual component of amylase given the designation "Y." This component had also been noted in some human milk samples. In one of the lung cancer patients, an isoamylase was found in the serum and urine after radiation treatment that was close to but not identical to the Y isoamylase in chromatographic position. Although a relationship of isoamylase component Y to generating tissue is suggested by these findings, such a relationship of isoamylase component Y to generating tissue is suggested by these findings, such a relationship remains to be proven.

Amylases↗
PubMed1976

Nonpancreatic-type hyperamylasemia associated with pancreatic cancer.

Isoamylase analysis of the serum and urine of a patient with anaplastic spindel cell carcinoma of the pancreas revealed that virtually all of the serum amylase and almost all of the urine amylase behaved chromatographically as the salivary (S) type. Both the serum and urine amylases were bound by a substance derived from a macroamylase complex which had been shown to bind only salivary amylase and to lack any affinity for pancreatitis (P) type amylase. The ratio of amylase to creatinine clearance was markedly increased (12.5%) without evidence of acute pancreatitis at autopsy and despite the presence of only a minute amount of P-type isoamylase in the serum.

Amylases↗
PubMed1976

Hyperamylasemia in heroin addicts. Characterization by isoamylase analysis.

Hyperamylasemia was noted in 17 (19%) of a group of 91 hospitalized heroin addicts. Thirteen of the 17 were in acute respiratory distress (12 with so-called "heroin lung" syndrome and one with status asthmaticus). Isoamylase analysis in the hyperamylasemic patients demonstrated S-type isoamylase dominance in 15, P-type isoamylase dominance in one and essentially equivalent P- and S-type isoamylase elevations in one. It would appear from these data that the hyperamylasemia after heroin in most persons addicted to the use of this drug arises from the sources other than the pancreas. Changes in the lungs occurring in association with heroin addiction seem to have an important role among the possible contributory factors.

Adolescent↗
PubMed1975

Serum amylase changes during pregnancy.

Study of the behavior of serum amylase activity in 200 preganant women in various stages of pregnancy indicated that: (1) serum amylase rises gradually during pregnancy until the twenty-fifth week and thereafter falls slightly; (2) serum amylase values may be found in normal pregnant women during the second and third trimesters that exceed those in normal men and nonpregnant women; (3) during the second trimester of pregnancy there may be an alteration in the relative distribution of the pancreatic and salivary-type isoamylases with the salivary type tending to dominate. Knowledge of these changes is of importance in the clinical assessment of serum amylase values in pregnant women complaining of abdominal pain and other symptoms suggestive of complicating acute pancreatitis. An explanation for the observed changes is not readily available and further study is required.

Adolescent↗
PubMed1975

Does human pancreas contain salivary-type isoamylase?

Amylase isoenzyme analysis was made of extracts of normal human pancreatic tissue by first conducting ion exchange chromatography of the purified material. This gave evidence of only pancreatic type (P-type) isoamylase for all purposes. However, when effluent fractions in which salivary type isoamylase would ordinarily be expected to be present were harvested, pooled, concentrated, and rechromatographed, the pancreatic extracts were found to contain some salivary type (S-type) isoamylase. The latter accounted for approximately 0-8 to 1-7% of the total recovered amylase activity. This finding of S-type isoamylase in normal human pancreas potentially has important bearing on the interpretation of isamylase analysis.

Adult↗
PubMed1975

Clinical application of amylase isoenzyme analysis.

Amylase isoenzyme analysis of serum, urine and other bodily fluids using dextran gel and ion exchange chromatography provides a new dimension in the identification of pancreatic disease. Examples are described that illustrate the clinical application of such analysis and the usefulness of the information that may be derived.

Adult↗