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L Franco

Publications and source records attributed to L Franco.

140 records · Page 8Linked to original sources
PubMedDate not supplied

A new glucose-repressible gene identified from the analysis of chromatin structure in deletion mutants of yeast SUC2 locus.

We have previously shown that some changes occur in the chromatin structure of the 3' flank of the yeast SUC2 gene in going from a repressed to an active state. In an attempt to find out the causes of these changes, we have carried out experiments in which mutant copies of SUC2 locus lacking either 5' or 3' flanks have been analysed for their transcriptional activity and chromatin structure. These experiments allowed us to discard any relationship between SUC2 transcription and chromatin changes within its 3'flank. Sequencing of this flank and mRNA analysis, however, resulted in the location of a putative peroxisomal 3-oxoacyl-CoA thiolase gene (POT1), which is repressible by glucose. The disruption of the gene produced a yeast strain unable to use oleic acid as a carbon source. This is the first time that chromatin structure analysis has permitted the identification of a new gene.

Acetyl-CoA C-Acyltransferase↗
PubMedDate not supplied

Anti-ulcer activity of carbenoxolone and ISF 3401 on PGE2 release in rat gastric mucosa.

We have investigated the ability of carbenoxolone and ISF 3041, a new carbenoxolone derivative, to protect the rat gastric and intestinal mucosa against lesions induced by acetylsalicyclic acid (ASA) and indomethacin. Moreover, we determined the capacity of the rat gastric mucosa to release PGE2 both in vitro and ex vivo, in the presence or absence of carbenoxolone or its analogs. These compounds are effective against lesions induced by ASA and intestinal damage induced by indomethacin. The amount of PGE2 obtained from incubated rat gastric mucosal pieces by in vitro and ex vivo indicate that carbenoxolone and ISF 3401 cause a concentration related increase of PGE2 with exception of the highest concentration. Increased prostaglandin content of gastric mucosa can partly explain the gastric and intestinal protection of these compounds and additional mechanisms could be involved in this action.

Animals↗
PubMedDate not supplied

The perirhinal cortex and long-term familiarity memory.

To analyse the functions of the perirhinal cortex, the activity of single neurons in the perirhinal cortex was recorded while macaques performed a delayed matching-to-sample task with up to three intervening stimuli. Some neurons had activity related to working memory, in that they responded more to the sample than to the match image within a trial, as shown previously. However, when a novel set of stimuli was introduced, the neuronal responses were on average only 47% of the magnitude of the responses to the set of very familiar stimuli. Moreover, it was shown in three monkeys that the responses of the perirhinal cortex neurons gradually increased over hundreds of presentations (mean = 400 over 7-13 days) of the new set of (initially novel) stimuli to become as large as those to the already familiar stimuli. Thus perirhinal cortex neurons represent the very long-term familiarity of visual stimuli. Part of the impairment in temporal lobe amnesia may be related to the difficulty of building representations of the degree of familiarity of stimuli. A neural network model of how the perirhinal cortex could implement long-term familiarity memory is proposed using Hebbian associative learning.

Animals↗
PubMedDate not supplied

Safety evaluation of chronic fluconazole therapy. Fluconazole Pan-American Study Group.

The possible adverse effects of chronic, high-dose fluconazole therapy are detailed from analysis of a multicenter, dose-escalating study of the therapy of invasive mycoses. Ninety-three adult patients were studied, 48 of these received > or = 6 months therapy and 20 received > or = 1 year. Fifty-eight patients received > or = 300 mg/day, and 7 received > or = 600 mg/day. One patient received 1,997 g over 86 months. Twenty-seven percent experienced possible symptomatic side effects, which resulted in 2 patients discontinuing therapy, and 42% had asymptomatic laboratory abnormalities, none of which were progressive. Headache, hair loss and anorexia were the most common symptoms experienced (each by 3% of patients), and eosinophilia and aspartate aminotransferase increases were the most common laboratory findings (12 and 10%, respectively). Fluconazole appears well tolerated and safe in these doses and durations.

Adult↗
PubMedDate not supplied

[Treatment of headaches in general practice. A therapeutic study (author's transl)].

A therapeutic study was conducted by practitioners working in a group practice, to evaluate the activity of tiapride in treating headaches, over a period of 1 year. The results were satisfactory in 60.6% of cases, especially in patients with headaches of psychogenic or digestive origin. The dosage was 150 to 200 mg orally, and the product was well tolerated apart from somnolence which was noted in about 1 case out of 4.

Adult↗