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Biomedical subjects

L Ferrer

Publications and source records attributed to L Ferrer.

At least 73 records · Page 4Linked to original sources
PubMed1994

Pathological findings in a cat with cryptococcosis and feline immunodeficiency virus infection.

This report describes the gross, histopathological, immunocytochemical and electron microscopic findings in a cat with systemic cryptococcosis and feline immunodeficiency virus (FIV) infection. Lymphadenopathy and cloudiness of leptomeninges were the major gross findings. Numerous cryptococcal yeasts were found in lymph nodes, brain, and lung, and were less common in the kidney and the eye. The inflammatory reaction varied in intensity and cell type (mononuclear through granulomatous) depending on the organ involved. Yeasts were mainly within phagocytes as revealed by electron microscopy. Some inflammatory cells were immunocytochemically-stained with anti-CD3 antibodies.

Animals↗
PubMed1993

Canine cutaneous mast cells dispersion and histamine secretory characterization.

In view of the high incidence of canine cutaneous atopic disease and the relevance of mast cells to its pathogenesis, it was considered important to isolate firstly cutaneous mast cells from normal dog skin and to assess the histamine secretory activity, as this can be further used as a tool for the study of canine skin mast cell pharmacology in cutaneous atopy. The procedure for canine dermal mast cell dispersion following a skin enzymatic digestion (as for previous human skin mast cell dispersion methods) is described in detail. The number of canine cutaneous mast cells yielded per gram of skin was 2.31 +/- 0.21 x 10(5) representing 1.00% of the total cutaneous cells. The total histamine content per mast cell is 4.93 +/- 0.39 pg. Net histamine release owing to stimulation by calcium ionophore A23187 (1 microM) and concanavalin A (1 mg ml-1) was respectively 32.17 +/- 3.56% and 20.39 +/- 2.41% of the total amount per cell. Viability and reactivity to both stimuli of dispersed cutaneous mast cells were similar to the results found in humans. The present study allows further research on the role of mast cells immunopharmacology in allergy by investigation of cells isolated from canine skin in naturally occurring or experimentally induced atopy in the dog to be undertaken.

Animals↗
PubMed1993

[Indirect calorimetry and nitrogen balance in patients with elective craniotomy].

The aim of this study was to assess resting energy expenditure and nitrogen excretion in patients with cranial injury. In 25 patients subjected to elective craniotomy and 23 healthy volunteers, energy expenditure was measured using indirect calorimetry and compared with estimations according to Harris-Benedict equations. During the second postoperative day, measured energy expenditure in patients was 100 +/- 19% (range 66-185) of estimation and 115 +/- 22% (range 76-159%) of measured values in control subjects. In sedated patients with flaccid muscular paralysis, measured energy expenditure was 82 +/- 12% of estimation and 94 +/- 15% of measured values in control subjects. Urinary urea nitrogen excretion in patients was 10.2 +/- 5.4 g/day and catabolic index was 6.8 +/- 5.1. Patients receiving corticoids had increased urinary urea nitrogen excretion and no differences in measured energy expenditure, compared to patients not receiving this medication. It is concluded that the great variability in measured energy expenditure and its lack of correlation with the degree of catabolism, set forth the need to perform an indirect calorimetry in these patients to plan an adequate nutritional support.

Adolescent↗
PubMed1993

[Protein and energy requirements in patients with severe head injury].

The aim of the present study was to assess the effect of severe head injury over resting energy expenditure and nitrogen losses. Eight patients with severe head trauma (mean Glasgow scale of 4.9 +/- 1.6), subjected to emergency craniotomy were studied; four patients had septic complications and none received steroids. Energy expenditure was measured using indirect calorimetry and nitrogen losses were assessed measuring 24 h urea nitrogen excretion on days 1, 4, 7 and 10 of the postoperative period. Twelve healthy males composed the control group. Measured energy expenditure in patients, evaluated in 22 occasions, was 35 +/- 9.8 Kcal/kg/day or 145 +/- 41% of the estimation according to Harris-Benedict equations, compared to 22.0 +/- 4.6 Kcal/kg/day or 89.9 +/- 17.5% in the control group (p < 0.001). 24 h urinary urea nitrogen excretion in patients was 18 +/- 12 g/day (range 5.2-46.9) and the catabolic index was 7.4 +/- 13.5. It is concluded that, due to the great individual variability in measured energy expenditure in these patients and their hypercatabolism, indirect calorimetry and nitrogen balance studies become useful tools to perform an adequate nutritional support.

Adolescent↗
PubMed1992

Immunocytochemical detection of amylase, carboxypeptidase A, carcinoembryonic antigen and alpha 1-antitrypsin in carcinomas of the exocrine pancreas of the dog.

The immunocytochemical detection of amylase, carboxypeptidase A, alpha 1-antitrypsin, carcinoembryonic antigen (CEA) and keratin in normal canine pancreatic tissue and in carcinomas of the exocrine pancreas of the dog is described. In the normal pancreas, the acinar cells contain amylase, carboxypeptidase and alpha 1-antitrypsin. The pancreatic ducts react with the antikeratin antibody. Twelve out of 14 pancreatic exocrine carcinomas showed immunoreaction with antiamylase antibody, and 10 with anticarboxypeptidase antibody. Five neoplasms reacted with anti-CEA antibody and three with the anti-alpha 1-antitrypsin antibody. It was not possible to find any systematic difference in the immunocytochemical profiles of acinar, tubular and undifferentiated carcinomas. These results indicate that immunocytochemical marking of amylase and carboxypeptidase is of value in the diagnosis of pancreatic neoplasms in the dog, especially if metastasis is the only material available for study and the tumour does not show any diagnostic feature on routine light microscope preparations.

Adenocarcinoma↗
PubMed1992

Pathologic and immunocytochemical studies of morbillivirus infection in striped dolphins (Stenella coeruleoalba).

Hundreds of striped dolphins (Stenella coeruleoalba) died along the Spanish Mediterranean coast during the second half of 1990. We necropsied 58 dolphins. Partial collapse of the lungs with patchy atelectasis, subcutaneous edema, icterus, and stomatitis were the most prominent gross morphologic changes. Histologically, a bronchiolo-interstitial pneumonia was the most frequent lesion (72% of the animals). It was characterized by hyperplasia of alveolar epithelial type II cells and formation of multinucleate syncytia in alveolar and bronchiolar lumina. Other prominent lesions were encephalitis (69%), lymphoid depletion, and formation of multinucleate syncytia in the cortex of lymph nodes. The distribution of morbillivirus antigen was investigated in 23 well-preserved dolphins using a monoclonal antibody against the hemagglutinin glycoprotein of phocine distemper virus. Positive immunostaining was found in brain (77%), in lung (70%), and in mesenteric (61%), mediastinal (47%), and prescapular (45%) lymph nodes. Phocine distemper virus antigen was demonstrated less frequently in trachea, stomach, biliary epithelium, intestine, kidney, and mammary gland. Necrotizing-hemorrhagic pneumonia and encephalitis due to Aspergillus fumigatus were seen in three dolphins, whereas two animals had lesions of toxoplasmosis. Changes in our dolphins were similar to those caused by distemper in seals and porpoises. The origin of the dolphin virus and the relationships among dolphin, seal, and porpoise morbilli viruses are unknown.

Animals↗
PubMed1992

Histochemical and immunohistochemical study of the mucosal lymphoid system in swine.

Enzyme histochemical and immunohistochemical techniques were used to examine palatine tonsils and aggregated lymphoid follicles (Peyer's patches) of the ileum in 6- to 9-day-old and in 6-month-old pigs. Histochemical techniques were used to detect alpha-naphthyl-acetate esterase (ANAE), alpha-naphthyl-butyrate esterase (ANBE), beta-glucuronidase, adenosine triphosphatase (ATPase), and acid phosphatase (AcP). Nonspecific esterases (ANAE, ANBE) were detected in macrophages, T-cell area lymphocytes, eosinophils, fibroblastic reticular cells (FRC), follicular dendritic cells (FDC), and interdigitating cells (IDC). beta-Glucuronidase reactivity was strong in macrophages, eosinophils, FDC, and IDC, and weaker in FRC. Adenosine triphosphatase reactivity was detected in B-cell area lymphocytes, FDC, FRC, and IDC. Cell types with acid phosphatase reactivity were macrophages, FDC, FRC, and IDC. Nonepithelial cells of tonsils and aggregated lymphoid follicles of the ileum had similar enzymatic reactions. In Peyer's patches, however, epithelial cells were positive for all enzymes studied; in tonsils, only nonspecific esterases were detected. Immunoperoxidase techniques were used to detect S-100 protein and cytoplasmic immunoglobulins (IgG, IgM, and IgA). The S-100 protein was detected in lymphocytes, FDC, and FRC of tonsils and Peyer's patches; in tonsillar epithelial and endothelial cells; and in IDC of Peyer's patches.(ABSTRACT TRUNCATED AT 250 WORDS)

Acid Phosphatase↗
PubMed1991

Canine leishmaniasis associated with systemic vasculitis in two dogs.

Two cases of canine leishmaniasis associated with systemic necrotizing vasculitis are described. The main macroscopic lesions were haemorrhagic in type; histopathological changes confirmed a vascular lesion affecting small arteries of several organs (skin, intestinal tract, kidney, urinary bladder, mesenteric lymph nodes, adrenal gland, myocardium, lung, eye and choroid plexus). The presence of the parasite was confirmed with a specific immunocytochemical stain. The possibility of an immunological aetiology of the vasculitis and its classification is discussed.

Animals↗
PubMed1991

[Histochemical and immunohistochemical study of the lymphoid tissue of swine: lymphatic ganglia, spleen and thymus].

Immunohistological (S-100, cIg) and enzyme histochemical (ANAE/ANBE, beta-glu, ATPase, AcP) investigations were carried out to identify lymphocyte and reticular cell subpopulations "in situ", in pig lymphoid tissue (lymph node, spleen, and thymus) of a 6 months old group, and a 6-9 days old one. By means of immunohistological techniques, in the 6 month old pigs we could detect S-100 protein (PAP), chiefly in T-areas lymphocytes, but we also found some S-100 positive lymphocytes in spleen follicles. Also S-100 protein were detected at Follicular Dendritic Cells (FDC) in lymph node and spleen; and Reticular Fibroblastic Cells (RFC) only in the first one. Finally, S-100 were noted in Hassall corpuscles (thymus), nervous fibres, and endothelial cells too. Using PAP (IgG, IgM) and IPI (IgA) techniques we could detect lymphocyte cytoplasmatic surface immunoglobulins (cIg) in lymphocytes, lymphoblastoid and plasmacytoid cells in nearly all tissue compartments. By means of histochemical techniques we could identify T-area lymphocytes ANAE/ANBE and beta-glu positives (cytoplasmatic spots) and B-area lymphocytes ATPase positive; macrophages, and polymorphonuclear eosinophiles PHNE being ANAE/ANBE and beta-glu positives (diffuse cytoplasmic stain); and Hassall corpuscles ANAE/ANBE and AcP positives. Concerning to reticular cells, we found FDC and RFC in lymphoid follicles, and Interdigitating Reticular Cells (IDC) in lymphoid diffuse tissue, with enzyme activity (all the enzymes studied) in nearly all the cases. In piglets, the immunohistological and histochemical pattern was nearly the same.

Animals↗
PubMed1991

[Vibration and acoustic stimulation during labor with external monitoring. Pathology of fetal heart rate].

Vibratory acoustic stimulation was done in patients with an abnormal fetal heart rate tracing in external continuous electronic monitoring during labor, correlating with Apgar score, meconium staining and umbilical cord pH, describing a 83.3% sensitivity, 81.3% specificity and 62.5% positive predictive value. The authors recommend the use of vibratory acoustic stimulation in those cases with an abnormal fetal heart rate tracing in external continuous electronic monitoring, when we can not install internal electronic monitoring or obtain a fetal blood pH on assessment of fetal well-being.

Acoustic Stimulation↗