A variant of the solid-phase radioimmunoassay (RIA) has been developed for the detection of specific immunoglobulins in laboratory animals infected with Pseudomonas pseudomallei. The proposed method has advantages over the indirect hemagglutination test and the enzyme immunoassay in its sensitivity and specificity. The newly developed RIA variant, based on group-specific antigens 6 + d, makes it possible to classify the strain causing the disease with the Asian or Australian serovar of P. pseudomallei according to the composition of detected immunoglobulins.

Animals↗

PubMedDate not supplied

[The results of a 15-year study of the persistence of Yersinia pestis L-forms].

New diagnostic preparations for the detection of L-forms, immunoglobulin erythrocyte diagnosticum and luminescent antimembrane immunoglobulins, are described. Proofs indicating that Y.pestis L-forms are constantly isolated from wild rodents and their ectoparasites in the natural foci of plague are given. To control the effectiveness of the sanitation of such natural foci, the use of the radioimmunoassay and the enzyme immunoassay, known to be the most sensitive techniques, are recommended. The facts indicating that Y.pestis contaminating ticks and fleas are converted into L-forms, capable of persisting for several years, are presented. After their reversion to back the initial rod-shaped form the transmission of infection is possible. A suggestion is put forward that the persisting forms of Y.pestis play an important role in the preservation of this infective agent in the natural focus of infection.

Animals↗

PubMedDate not supplied

[A gas chromatographic analysis of the fatty acid composition of Yersinia pestis].

The fatty acid composition of Y. pestis strains, the causative agent of plaque, has been studied. Y. pestis cells have been found to contain great amounts of palmitoleic acid, methylenehexadecanoic acid, oleic acid with elaidic acid, palmitic acid and pentadecanoic acid. Lauric acid, myristic acid, 3-oxymyristic acid and methyleneoctadecanoic acid have been detected in moderate amounts. Vaccine strains, virulent museum and newly isolated strains, while differing in their antigenic structure, have proved to be uniform in their fatty acid composition. In Y. pestis cels, grown on a solid medium for a longer period and at higher temperature, an increased proportion of saturated acids is observed.

Chromatography, Gas↗

PubMedDate not supplied

[The fatty acid composition of the cellular structures of Yersinia pestis].

Fatty acid composition of lipopolysaccharides (LPS) and cytoplasmic membranes (CPM) of Yersinia pestis [correction of the plaque microbe] has been studied. Concentration of certain acids in the LPS content proved sharply different and thus the strains were separated into groups. High content of 3-oxymiristinic acid was a distinction of vaccine strains. Virulent strains of one of the groups regularly differed from the rest of virulent strains by high content of laurinic acid. Laurinic and two nonidentified acids, which were not found in the virulent cultures, were present in CMP lipids of vaccine strains.

Bacterial Vaccines↗