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Biomedical subjects

L Donato

Publications and source records attributed to L Donato.

142 records · Page 8Linked to original sources
PubMedDate not supplied

Prevalence of opiate use among young men in Italy, 1980 and 1982.

An epidemiological study on opiate use among young men undergoing examination for army draft was carried out in 1980 and 1982. The following six cities and surrounding provinces were selected for the study: Bologna, Florence, Genoa, Milan, Rome and Turin. There were 15,266 respondents in 1980 and 15,874 in 1982. For each respondent, a urine sample was tested for the presence of opiates and a questionnaire on demographic and socio-economic variables was completed. The study revealed 247 opiate cases with a prevalence rate of 1,618 per 100,000 in 1980, and 339 opiate cases with a prevalence rate of 2,136 per 100,000 in 1982. The number of opiate cases and demographic and socio-economic characteristics of respondents varied greatly from province to province. The group of unemployed respondents had significantly higher prevalence rates than the other occupational groups. A methodology was used that made it possible to estimate the number of opiate abuse cases that did not come to the attention of the authorities. On the basis of the study it was estimated that there were 68,000 opiate users in 1980 and 92,000 in 1982 in the entire country.

Adolescent↗
PubMedDate not supplied

Enzymatic hydrolysis of DNA using a membrane bioreactor.

Aim of this work was a kinetic study of the deoxyribonuclease I (E.C. 3.1.21.1.) reaction in several conditions. Optimal reaction conditions with enzyme free in solution were determined. DNase activity was studied as function of several parameters such as pH, enzyme concentration, substrate concentration, cofactor concentration. The Km value of the free enzyme was also determined. Subsequently, the possibility to carry out the reaction in systems in which the enzyme was immobilized on the inner surface of the polymeric membranes was evaluated and realized. Experimental procedures, in these new conditions, permitted to determine the mass of immobilized enzyme and the percentage of the reaction product recovery. Results showed that the immobilization procedure determines a decay of the initial activity of enzyme, but there is an important increase of enzyme stability.

Animals↗