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Biomedical subjects

L Deiana

Publications and source records attributed to L Deiana.

24 records · Page 2Linked to original sources
PubMed1983

[Embryo-toxic effects of nickel sulfate in Paracentrotus lividus: preliminary observations].

A primary objective of the work described here was to determine the embryo-toxic effects induced by Nickel Sulfate upon the development of the Paracentrotus Lividus. Different concentrations of Nickel Sulfate have been dissolved in containers with not foul sea-water. The fecundation took place in the containers and verified by constant observations with a light microscope. The development of the Paracentrotus 1. was totally blocked at a concentration of 0.5 M.10(-2) of Nickel Sulfate, while an enormous development was observed at concentrations of 0.5 M.10(-3) and 0.5 M.10(-4). Finally, a concentration of 0.5 M.10(-5) caused a slow development. A normal development was observed only at a concentration of 0.5 M.10(-6). Our results support various hypotheses on the mechanism of action of the Nickel Sulfate as an inhibitor of the normal development.

Animals↗
PubMed1982

[Immunohistochemical observations on tubulin and alpha actinin during mitosis of cultured fibroblasts].

In this work we studied alpha-actinin and tubulin sites in rabbit fibroblasts in culture. Antibodies anti-alpha-actinin were used for indirect PAP-reaction while antibodies anti-tubulin were used for indirect immunofluorescence method. The observations were carried out by light microscopy, phase-contrast and interference-contrast microscopy, with regard to actinin, and by fluorescence microscopy, with regard to tubulin. During the early mitotic phase, alpha-actinin is localized all over the cell membrane of the fibroblasts, forming a sort strong protective cap, while during diacinesis it forms only rings, localizing below the cell membrane and the philopodia. Thus tubulin forms the bundle fibres during mitotic phases.

Actinin↗
PubMed1982

[Alpha-actinin sites on microvilli of the rabbit small intestine demonstrated by immunoperoxidase].

When Mooseker and Tilney showed the microvilli system must be ascribed among non-muscle contractile systems, they have proposed a contractile scheme formed by actin, miosin and probably alpha-actinin. The scheme proposed by these AA. consists of actin filaments oriented and running from the border to the base, bound to the border by a plate of dense substance formed by -actinin and, to the base, other perpendicular miosin filaments. In our opinion, Mooseker and Tilney research lacks a demonstration regarding alpha-actinin and that is reason why we have decided to display this protein in the microvilli by immunoperoxidase techniques. Considering our results we can definitively confirm the full validity of the contractile scheme of Mooseker and Tilney.

Actinin↗
PubMed1982

[Dynamic aspects of alpha-actinin in cultured rabbit fibroblasts studied with immunofluorescence].

In this work we study alpha-actinin sites in rabbit fibroblasts in culture. For this purpose we extracted alpha-actinin from rabbit striated skeletal muscle and produced the relative antibodies in sheep. Electrophoresis was performed, on PAA slab-gel and the immunodiffusion test on agarose slab-gel. Antibodies were used for direct and indirect immunofluorescence techniques by TRIC and FITC. We noticed that in young fibroblasts alpha-actinin is concentrated in perinuclear regions, while in adult fibroblasts it is scattered, more or less evenly in the cytoplasm and above all on the plates which link the cell membrane to the substractum. Both the direct immunofluorescence method with antibodies anti-alpha-actinin, marked by TRITC, and the indirect one with sheep IgG, marked by FITC, gave identical results.

Actinin↗
PubMedDate not supplied

Metabolic and renal effects of Laevo-carnitine and propionyl-carnitine in rats with subtotal nephrectomy.

The renal and metabolic effects of chronic carnitine administration were evaluated in the early stages of experimentally-induced renal failure. Laevo-carnitine (n = 5), Propionyl-carnitine (n = 5) both at 200 mg kg-1 of body weight, or vehicle (physiological saline solution, 0.4 ml kg-1 body weight, n = 5) were administered daily for 3 days prior to 2/3 nephrectomy and for 25 days thereafter, by intraperitoneal route. At the end of the experiment, no significant differences were found in systolic blood pressure and heart rate among groups. During the 25 days after nephrectomy, body weight increased by 71 +/- 13 g in the control group and by 50 +/- 26 g and 42 +/- 9 g in Laevo-carnitine and Propionyl-carnitine groups, respectively (P < 0.05 vs control for both comparisons). Urinary sodium excretion was increased in carnitine-treated rats (Laevo-carnitine: from 1.03 +/- 0.3 to 1.36 +/- 0.3 mEq day-1, Propionyl-carnitine: from 1.2 +/- 0.2 to 1.66 +/- 0.2 mEq day-1, P < 0.05 for both comparisons), but not in those given vehicle. Twenty-five days after nephrectomy, plasma creatinine was lower in carnitine-treated rats (Laevo-carnitine: 0.98 +/- 0.12 mg dl-1, Propionyl-carnitine: 1.06 +/- 0.15, vehicle: 1.52 +/- 0.09, P < 0.05 vs control for both comparisons). Plasma triglycerides and VLDL were decreased by nephrectomy and this effect was prevented by carnitine treatment. The data indicate that the carnitine blunts the increase in plasma creatinine that occurs early after partial nephrectomy and normalizes the plasma lipoprotein pattern. Thus, carnitine might protect against the development of renal failure in this experimental model.

Animals↗