Biomedical subjects

L Davidsson

Publications and source records attributed to L Davidsson.

At least 55 records · Page 3Linked to original sources

PubMed1989

Manganese retention in man: a method for estimating manganese absorption in man.

Whole-body retention of 54Mn was studied in man by measurements in a sensitive whole-body counter after intake of an extrinsically labeled infant formula. Reproducible retention figures at day 10 were observed after repeated administrations to six subjects; 2.3 +/- 1.1, 3.3 +/- 3.1, and 2.4 +/- 1.4% (means +/- SD) for three separate occasions. Interindividual variation of manganese retention after intake of the same labeled diet was, however, shown to be substantial. Retention at day 10 was 2.9 +/- 1.8% (means +/- SD) and varied from 0.6 to 9.2% when measured in 14 healthy subjects. Large interindividual variations in rate of excretion were observed. The retention measurements for days 10-30, however, could be closely fitted to a single exponential function for each individual. The results regarding intraindividual and interindividual variation in Mn retention indicate that factors influencing Mn absorption can be identified only by repeated administrations using each subject as his/her own control.

Administration, Oral↗

PubMed1989

Identification of transferrin as the major plasma carrier protein for manganese introduced orally or intravenously or after in vitro addition in the rat.

It is known that the metabolic handling of manganese (Mn) introduced via the diet or by intravenous injection is quite different. We hypothesized that this difference could be due in part to different proteins carrying Mn in plasma that could affect tissue uptake and retention. To test this idea, 54Mn was administered orally or intravenously to rats, and blood samples were taken by cardiac puncture at various time points postdosing. Plasma proteins were separated using fast protein liquid chromatography with a combination of anion exchange and gel filtration columns. Using these methods, independent of the route of 54Mn administration, transferrin was identified as the major Mn-binding protein in plasma. The identity was further confirmed by SDS-polyacrylamide gradient gel electrophoresis and Western blotting. These results conclusively show that 54Mn in plasma is carried by transferrin, regardless of route of administration and time postdosing.

Administration, Oral↗

PubMed1988

Intrinsic and extrinsic labeling for studies of manganese absorption in humans.

A dual-radioisotope method was used to simultaneously study whole-body manganese retention from a chicken liver based meal intrinsically labeled with 54Mn and extrinsically labeled with 52Mn. Manganese retention was monitored in a sensitive whole-body counter during approximately 30 d in six young adult women. Both radioisotopes were retained to a similar degree and excreted at identical rates. Retention at d 5 was 14.4 +/- 10.3 and 14.0 +/- 9.9% while retention at d 10 was 5.0 +/- 3.1 and 5.0 +/- 3.0% (X +/- SD) for 54Mn and 52Mn, respectively. From these results we conclude that the intrinsic and extrinsic Mn isotopes did form a common pool before absorption. The results can therefore be regarded as a direct validation of the use of extrinsic labeling for studies of Mn retention for estimating Mn absorption in man.

Adult↗

PubMed1987

The effect of vegetables and beet fibre on the absorption of zinc in humans from composite meals.

1. The absorption of zinc in humans from composite meals, was determined by extrinsic labelling of the meals with 65Zn and measurement of the whole-body retention of the radioisotope. 2. Low-Zn (mean 25 mumol) chicken meals with 150 g white bread or 225 g potatoes, carrots, turnips, cabbage or green peas were studied. The effect of a beet-pulp-fibre preparation used as a breakfast cereal, in bread and as a meat extender on Zn absorption was also studied. 3. The mean percentage absorption from the chicken meals with white bread, carrots and cabbage was significantly different from the meals with potatoes, turnips and green peas. When the amount of Zn in the meals was taken into account a slightly higher absorption was observed from the white-bread meal compared with the meals with potatoes and cabbage, while no differences were seen between the vegetable meals. 4. The beet-pulp-fibre preparation did not affect the extent of Zn absorption when used as a meat extender. The absorption of Zn was higher when the beet fibre was included in bread than when used as müesli. 5. The results obtained suggest that, besides the low-Zn content in vegetables, a large intake of vegetables or a pure-vegetable-fibre preparation has no significant effect on Zn availability from animal-protein-based meals.

Adult↗

PubMed1987

Retention of selenium (75Se), Zinc (65Zn) and manganese (54Mn) in humans after intake of a labelled vitamin and mineral supplement.

The whole body retention of 75Se, 65Zn, and 54Mn after intake of a labelled vitamin and mineral supplement was followed in 12 healthy volunteers. The supplement had a vitamin and mineral content according to recommended dietary allowances or the so-called "safe and adequate levels" for trace elements, including 15 mg of zinc as zinc citrate, 50 micrograms of selenium as sodium selenite and 2.5 mg of manganese as manganese sulphate. The supplement was taken either in the fasting state or together with a light meal. Retention day 14 was 48 +/- 6%, 33 +/- 6% and 5 +/- 2% (mean +/- SD) for selenium, zinc and manganese, respectively, when the supplement was taken fasting and 45 +/- 3%, 8 +/- 1% and 1.0 +/- 0.2% when it was taken with food. During day 1-14, 27%, 1% and less than 0.01% of the administered selenium, zinc and manganese radionuclides, respectively, were excreted in the urine. Based on the rate of turnover of the radionuclides and the urinary losses of 75Se, the absorption of selenium, zinc and manganese from the supplement was estimated to be 89 +/- 5%, 38 +/- 7%, 9 +/- 3% (mean +/- SD) in the fasting state and 87 +/- 4%, 10 +/- 2% and 2 +/- 1% with food. These results indicate that when a supplement is taken with food the minerals are absorbed and metabolized in the same way as are native minerals in food. When the supplement is taken in the fasting state, the absorption of zinc and manganese can be substantially higher.

Absorption↗

PubMed1987

Zinc status and dark adaptation in patients subjected to total gastrectomy: effect of zinc supplementation.

Totally gastrectomized patients could be regarded at risk for development of nutritional deficiencies including trace elements. Sensitive indices for early detection of these deficiencies are lacking. In order to evaluate the nutritional status of trace elements in patients previously subjected to a total gastrectomy we studied the zinc status in 10 patients by determining serum zinc, urinary zinc excretion and also, by a simplified dark adaptation test, the effect on these parameters of 4 weeks of zinc supplementation. The serum zinc level but not the 24-h urinary zinc excretion was lower in gastrectomized patients compared to age-matched controls. A slower dark adaptation was observed in the former patients when measured in the non-fasting state but not when fasted. Dark adaptation was slower in the patients as well as the age-matched controls compared to younger healthy subjects. Zinc supplementation increased the serum zinc levels and the urinary zinc excretion in the gastrectomized patients but had no effect on dark adaptation.

Aged↗

PubMed1986

Manganese absorption and metabolism in man.

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Administration, Oral↗

PubMed1985

Oral iron, dietary ligands and zinc absorption.

The effect of iron on zinc absorption in humans was investigated by using 65Zn and whole-body counting after 2 wk. Increasing the molar ratio of ferrous iron (with ascorbic acid) to zinc from 1:1 to 2.5:1 did not affect absorption of zinc from water when given in a fasting state; 59 and 58% was absorbed, respectively. However, at an Fe:Zn ratio of 25:1, zinc absorption from water decreased significantly to 34%. When oral iron in the same ratio to zinc was given with a meal, no inhibitory effect was observed (25, 23 and 22%, respectively). Addition of the zinc ligand, histidine, to the water solution decreased the inhibitory effect of the higher dose of iron, resulting in a zinc absorption of 47%. Two weeks of iron preloading did not affect zinc absorption from water. The results demonstrate that when a multimineral supplement is taken on an empty stomach, excessive iron levels can negatively affect zinc absorption. Intake of the supplement with a meal or with a zinc ligand (such as histidine) may overcome this inhibitory effect.

Administration, Oral↗

PubMed1984

The effect of individual components of soy formula and cows' milk formula on zinc bioavailability.

Zinc absorption from human milk, cows' milk formulas, and soy formulas was studied in human adults by a radioisotope technique using 65Zn and whole body counting. Individual dietary components were investigated for effects on zinc absorption. Phytate was found to have a strong inhibitory effect on zinc absorption; addition of phytate to cows' milk formula (yielding a phytate concentration similar to that of soy formula) resulted in a decrease in zinc absorption from 31 to 16% similar to the absorption for soy formula (14%). Carbohydrate source, calcium, and zinc levels of the diet did not affect zinc absorption significantly. Iron supplementation of cows' milk formula decreased zinc absorption from 24 to 18% although this decrease was not found to be significant (p less than 0.1). Absorption of zinc from a whey-adjusted cows' milk formula was higher (31%) than from a nonmodified cows' milk formula (22%). Increasing the zinc supplementation level in cows' milk formula but not in soy formula increased zinc absorption to approximate that from breast milk. It is suggested that reduction of phytate content of soy formula may be a more effective avenue of modification than increased level of zinc supplementation.

Absorption↗

PubMed1983

Occurrence of gamma-trace in the calcitonin-producing C-cells of simian thyroid gland and human medullary thyroid carcinoma.

gamma-Trace, a microprotein occurring in neuroendocrine cells, was demonstrated by immunohistochemical technique to be present in the calcitonin-producing C-cells of normal simian thyroid gland and of human medullary thyroid carcinoma. A comparatively high concentration of gamma-trace was demonstrated in tissue extract of neoplastic C-cells. The immunoreactive protein found in the extract had a molecular weight and electrophoretic mobility characteristic for gamma-trace.

Adsorption↗

PubMed1982