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Biomedical subjects

L Danes

Publications and source records attributed to L Danes.

At least 37 records · Page 2Linked to original sources
PubMed1989

[Virologic and morphologic study in sudden and unexpected death in children].

The authors examined a group of 47 children who died at the age of 1-9 months, incl. 19 female and 28 male children. In 23 the death was described as sudden and in 24 as unexpected. From 19 dead children a virus was isolated: in 14 one or two serotypes of enterovirus were found, in two the virus of parainfluenza 3 in two adenoviruses (type 2, 7). A total of 46 viral strains were isolated. By immunofluorescence in tissues antigens of the parainfluenza virus 3 and adenovirus were detected. Immune complexes were not detected in tissues. The distribution of vaccination polioviruses in organs and tissues after a known interval following vaccination was recognized. In some of the infants a possible causal relationship between the virological and morphological findings was found and the authors discuss the possible pathogenetic role of the revealed changes in sudden death.

Female↗
PubMed1989

Serological survey of rubella in Yemen in 1985.

476 sera from subjects of 6 age groups were investigated using the HI test for rubella, the sera originating from 5 distinct regions of Yemen, namely highland, coastal the agricultural area Abijan, Vadi Hadramot and the capital Aden. The positivity rate among children aged 1-6 ys was 46.2%, the values obtained for girls aged 15-18, women in the 19-24 and 25-29 ys age group being 81%, 86.3% and 89.9%, respectively. When the findings of the immunological survey were evaluated with respect to the five different regions studied, the positivity rate in the highlands was found to be significantly higher among girls under 18 ys of age. The geometric means of HI titres for rubella antibodies proved to be the lowest in 1-3-year-old children (1: 59) and the highest in girls aged 15-18 ys (1: 163). The authors discuss the comparatively high risks of contracting rubella during pregnancy in four regions of Yemen.

Adolescent↗
PubMed1988

Cross reactivity among human enterovirus serotypes as revealed by microneutralization assay technique.

Paired or tripled serum specimens examined for cross reactions between poliomyelitis viruses and other non-polio enteroviruses were obtained from 356 patients (217 males and 139 females) with various clinical diagnoses (aseptic meningitis, meningoencephalitis, paretic neuro-infections, upper respiratory tract infections, bronchopneumonia) who were sampled in 1984 and 1985. The sera were examined by the microneutralization assay technique in flat-bottomed 96-well polyvinyl chloride plates against 100-300 TCD50 of these enterovirus serotypes: CA9, CB1-CB5, E1, E2, E4-E9, E17, E20, E24, E30, EV71 and polioviruses P1-P3. The tests were carried out on BGM or RD cells. Confirmed significant seroconversions to at least one enterovirus serotype were observed in 87 (24.2%) patients. Of these, 27 (31%) reacted by parallel significant or insignificant increases in the heterotypic antibody titre to poliovirus, which accounted for 7.6% of all patients examined. Heterotypic antibody responses may become source of errors in individuals examined for poliovaccine efficacy or the state of specific immunity response to polio, but are unlikely to discredit the overall outcomes of the seroepidemiological surveys conducted in the general population.

Antibodies, Viral↗
PubMed1988

Immunofluorescent IgG and IgM antibodies in human sera by enterovirus infections.

IgG and IgM antibodies to selected enterovirus serotypes (P2, CB 1-5, E2, 4, 20), to prototype collection strains and to fresh isolates were examined by immunofluorescence in paired sera of 11 children and 4 adults in whom enterovirus had been isolated, or a rise of specific antibodies had been proved by neutralization test. One six-month- and one seven-month-old child had antibodies of both classes to the isolated virus strain and to poliovirus only. Children from eight month onwards and adults had antibodies to the majority of enterovirus serotypes tested. Among all enteroviruses tested, heterologous reactions were observed not only with IgG but also with IgM class antibodies.

Adult↗
PubMed1986

Hemorrhagic fever with renal syndrome in Czechoslovakia: detection of antigen in small terrestrial mammals and specific serum antibodies in man.

A direct ELISA technique was used for demonstration of HFRS virus antigen in suspensions of the lungs obtained from 774 wild small mammals belonging to 13 zoological species trapped in 21 localities in Czechoslovakia between 1983 and 1984. Western serotype of HFRS virus was demonstrated in the lungs of 8 Microtus arvalis voles caught in the areas of central Bohemia, southern Moravia and western Slovakia, and in one Clethrionomys glareolus vole from the area of eastern Slovakia. The eastern serotype antigen was found in one Apodemus agrarius field mouse captured in the area of eastern Slovakia. The antigen titres as determined in the lungs of these rodents by the ELISA and RIA techniques were relatively high. Human sera examined for the presence of HFRS-specific antibody by the indirect fluorescence and RIA techniques were 76 serum specimens from chronic kidney patients treated by hemodialysis and 349 sera of adult males over 35 years of age coming from various districts of Bohemia and Moravia. Antibodies against HFRS virus antigen were found in 4 sera obtained from northern Bohemian men. The results provide the evidence which suggests that the natural foci of HFRS are likely to occur across much of the Czechoslovak territory, and confirms the focality of this infection in at least some of the localities characteristic in the 1950s and 1960s of the incidence of severe sporadic cases of HFRS.

Adult↗
PubMed1985

Neutralization microtest with human coxsackievirus and echovirus serotypes.

Sets of 600 single sera from healthy individuals of various age and 458 paired sera from patients treated for diseases of various etiology were examined using a neutralization microtest technique employing prototype collection strains CA9, CB1-CB5, E1-E9, E11-E14, E17, E19, E24 and E26. Totally, 34,862 monotype neutralization tests were carried out in this study. In the set of single serum samples the lowest proportion of sera reacting with any of the enterovirus serotypes used was encountered in the group of youngest children up to the age of 2 years. This group of children showed also the highest proportion of sera free of type-specific antibody. Taken together, these sera reacted most frequently with serotypes CA9 and CB4. In the set of paired sera significant rises in antibody titre to one enterovirus serotype were recorded in 105 instances, in association with simultaneous nonsignificant rises against one or several other serotypes in 64 instances. In additional 12 paired sera there was evidenced a significant rise of antibody titre to more than one serotypes, combined in 8 of these with simultaneous nonsignificant rises to further serotypes. This neutralization microtest technique with a set of enterovirus serotypes is believed to represent a servicable diagnostic tool in determining the cause of enteroviral infection, in spite of the estimated 10% failure to establish the exact serotype responsible. The results yielded by the serologic examination of single serum samples are not considered as reflecting the actual seroconversion rates in the general population.

Adolescent↗
PubMed1984

Poliovirus and echovirus survival in Tetrahymena pyriformis culture in vivo.

Axenic cultures of Tetrahymena pyriformis, strain I MT IV, grown in a defined medium at room temperature, were used to study interactions of these protozoa with vaccination strain L Sc 2ab of poliovirus type 1, vaccination strain P 712 of poliovirus type 2 and with type 30 echovirus, strain 480/78. T. pyriformis cultures in media containing 10(3.0) TCD50/1 ml of type poliovirus, 10(3.0) TCD50/1 ml of type 2 poliovirus or 10(2.5) TCD50/1 ml echovirus 30 and in virus-free medium did not differ one from another in their growth and die-away kinetics during the 21 days of observation. Two-day T. pyriformis cultures were infected with poliovirus 1 (initial concentration 10(3.2) TCD50/1 ml), and poliovirus 2 and echovirus 30 (initial concentrations 10(3.0) TCD50/1 ml). Viruses were titrated in test tube cultures of BGM cells. The supernatant fluid, standardized sediment and samples of control virus suspension free of protozoa were titrated after 0, 2, 6, 10, 13, 18, 28 and 30 days. Most of the virus in culture was found associated with the sediment, both in the period of active growth and during the die-away phase of T. pyriformis protozoa. The virus in sediment was present at higher titres and its survival time was longer than in virus in liquid phase. Thirteen days after the first contact between T. pyriformis and virus the sediment and supernatant fluid of the old protozoan culture and the T. pyriformis-free control viral suspension were taken and used as inocula for new two-day T. pyriformis cultures.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1984

Elimination of some enteroviruses in the excrements of experimentally infected rats (Rattus norvegicus) and gulls (Larus ridibundus).

Young rats of both sexes, weight 150-170 g, the first laboratory progeny of captured wild parent pairs, were used throughout this experiment. Rats in two experimental groups comprising a total of 34 animals were infected orally with type 2 poliovirus vaccine strain given in each group at doses of 500, 5000 or 50,000 TCD50. In the first experiment, the presence of poliovirus in rat excrements was detectable irregularly till day 13, in the second experiment till day 2 after infection. Small quantities of virus were also detectable from the colon and cecum wall, exceptionally from the mesenteric lymph node. The third experiment included 8 rats orally infected with 5,000 TCD50 of echovirus 30; at the lower dose of virus all excrement samples were culture-negative, at the higher dose the positive virus recovery was recorded in 3 animals one day after infection. Analogous experiments in the fifth group of rats orally infected with 5,000 TCD50 or 50,000 TCD50 of enterovirus 71 yielded much the same results; organs of further 6 animals infected intranasally with 5,000 TCD50 of this virus were culture-negative and no virus-related changes could be histologically demonstrated in these animals. The second part of this study included the experiments conducted on 17 young Larus gulls bred in the laboratory from eggs collected in a colony of free living birds. Groups of these gulls were orally infected with 500 or 5,000 TCD50 of one of the following viruses: type 1 poliovirus vaccine strain, type 3 poliovirus vaccine strain, echovirus 30, enterovirus 71 and coxsackievirus B4. All samples of gull excrements collected till day 7 or 20 postinfection were culture-negative. These results suggest that wild rats may play some role in the spread of human enteroviruses in the environment, but no such role could be demonstrated in the Larus gull.

Animals↗
PubMed1984

Polio antibody outfit of newborns and infants born after 20 years of mass vaccination with live poliovaccine.

Antibodies against type 1 and 2 poliovirus were tested in 250 infants and against type 3 poliovirus in 341 infants aged 0-13 months to compare the polio antibody outfit of newborns and infants born to three groups of mothers: (a) nonvaccinated, above 35 years of age; (b) vaccinated with attenuated poliovirus but having had chance of contact with wild poliovirus during childhood, age 22-35 years, and (c) vaccinated but not having had contact with wild poliovirus, below 22 years of age. Over 90% of newborns had specific neutralizing antibodies against all three poliovirus types. With increasing infant age the percentage of antibody-positive infants decreased: by age 9-11 months only 46%, 27% and 14% of infants displayed antibodies to polio-virus types 1, 2 and 3, respectively. Geometric mean titres decreased accordingly. Differences between infants born to mothers of the above three groups were nonsignificant: the results obtained suggest that future newborns will also possess satisfactory levels of antibodies acquired from mothers who have only had contact with attenuated vaccine poliovirus.

Adolescent↗