Search PubMed⌕ Search

Biomedical subjects

L Crawford

Publications and source records attributed to L Crawford.

At least 109 records · Page 6Linked to original sources
PubMed1983

Microsomal P-450 induction by some secondary products from thermal oxidation of dietary lipids: epidermal hyperplasia, mutagenicity and cytochrome P-450 activities.

Distillable secondary products from roasted fowl were found to be cytotoxic but not mutagenic when assayed with Salmonella typhimurium strains TA98, TA100 and TA1537. A crudely separated fraction of the volatiles produced focal hyperplasia and damage to the epidermis of the backs of mice. The volatiles also caused an apparent synthesis of non-constitutive forms of rat hepatic cytochromes P-450 which metabolize benzo[a]pyrene B [a]P differently from the constitutive P-450.

Administration, Topical↗
PubMed1982

Radioimmunoassay of the cellular protein p53 in mouse and human cell lines.

We have developed quantitative radioimmunological solid phase assays for the host protein p53 from mouse cells and from human cells. The first assay, for mouse p53, depends on having two monoclonal antibodies reacting with different determinants on the p53 molecule. With this assay we have shown that SV40-transformed cells have approximately 100-fold more p53 than untransformed mouse cells and that other transformed cells have intermediate levels. Embryonal carcinoma cell lines have approximately 50-fold less p53 than SV40-transformed cells. This is in contrast to the high levels of incorporation of [35S]methionine into p53 in these cells and indicates that metabolic labelling is not a valid approach for measuring p53 levels. The second assay, for human p53, required a different approach and made use of the anti-p53 antibodies detected in the sera of some breast cancer patients. Human tumour cell lines contained amounts of p53 varying from the high level seen in SV40-transformed human fibroblasts down to less than one hundredth of this amount. Normal human cells showed low levels of p53. The data confirm that many, but not all, human tumour cell lines contain more p53 than normal cells.

Animals↗
PubMed1982

Uniform nomenclature for monoclonal antibodies directed against virus-coded proteins of simian virus 40 and polyoma virus.

A uniform nomenclature has been agreed upon for monoclonal antibodies directed against virus-coded proteins of simian virus 40 and polyoma virus. Blocks of numbers from PAb1 to PAb999 have been allocated to workers involved in the isolation of monoclonal antibodies of this type. The correspondence between PAb numbers and previously used names is given.

Antibodies, Monoclonal↗
PubMed1982

Cellular proteins reactive with monoclonal antibodies directed against simian virus 40 T-antigen.

Several recently isolated monoclonal antibodies which reacted with simian virus 40 T antigens also reacted with proteins found in uninfected and untransformed cells. The proteins were different from each other, PAb419 reacting with a 35,000-molecular-weight protein, PAb427 reacting with a 75,000-molecular-weight phosphoprotein, PAb405 reacting with a 150,000-molecular-weight phosphoprotein, and PAb204 reacting with a 68,000-molecular-weight protein. It is suggested that although some of these cross-reactions may be fortuitous, they may, as an alternative, reflect similarities of shape and perhaps function between domains of the viral T antigen and the relevant host proteins.

Animals↗
PubMed1979

Some aspects of bile acid and urobilinogen excretion and fecal elimination in men given a rural Guatemalan diet and egg formulas with and without added oat bran.

Six healthy men were fed a formula diet with and without oat bran and a natural food diet typical of rural Guatemala. No significant difference in dye transit time was found between diets but the Guatemalan diet significantly decreased dye retention time and increased stool frequency. Serum cholesterol and triglyceride levels showed no significant differences among dietary treatments. Excretion of fecal bile acids significantly increased on the Guatemalan and oat bran diets, but fecal bile acid concentration was significantly lower only on the Guatemalan diet. Urinary urobilinogen excretion and fecal urobilinogen concentration were significantly lower with the Guatemalan diet.

Adult↗
PubMed1977

Antiarrhythmic action of lignocaine in early myocardial infarction. Plasma levels after combined intramuscular and intravenous administration.

In an attempt to find a regimen suitable for pre-hospital prevention of arrhythmias following myocardial infarction, the antiarrhythmic and pharmacokinetic effects of combining intravenous and intramuscular lignocaine have been studied. In nine patients with acute myocardial infarction, 100 mg of lignocaine was administered intravenously and 300 mg into the deltoid muscle. The antiarrhythmic effect was observed by continuous tape monitoring of the patients' rhythm before and after treatment. Plasma levels above 2 microng/ml were achieved within 1 min and maintained for 1 h in all patients; in seven this level was maintained for 2 h. A marked reduction in the occurrence of ventricular ectopic beats was observed in the first 15 min after treatment and was maintained for 90 min, but a significant effect was still present at 3 h. No serious side-effects were noted.

Arrhythmias, Cardiac↗
PubMed1975

Controlled study of the cytotoxic food test.

The cytotoxic food test was not found to be an accurate method for diagnosing atopic reactions to foods. Claims that the test correlated with other untoward reactions to foods (eg, headache, diarrhea, fatigue) could not be corroborated. The test itself is time-consuming, dependent on subjective interpretation, and inconsistent in results when repetitive runs are performed on the same patient.

Adolescent↗
PubMedDate not supplied

Clinical features and abnormal neutrophil function in disseminated staphylococcal disease.

Clinical features and immune status have been studied in seven previously healthy children with disseminated staphylococcal disease. Six of seven patients had a history of a viral-type illness before developing disseminated staphylococcal disease. Five patients had a petechial rash. Endocarditis occurred in three patients, two of whom had a cerebral embolism. All seven patients had an abnormal urinary sediment, and in two it was suggestive of glomerulonephritis. Two had transient renal failure. Three patients had evidence of pneumonia, one of the three developed pneumatoceles and one developed a pleural effusion. Four had osteomyelitis and/or septic arthritis. All patients had a transient abnormality of intracellular bacterial killing by neutrophils. One patient died. Three patients have residual valvular heart disease; one of the three patients has weakness of one arm and another has a seizure disorder. Cellular and humoral immunity in all six surviving patients is normal. We speculate that an antecedent viral infection temporarily suppressed neutrophil function and predisposed these children to secondary and severe staphylococcal disease.

Adolescent↗