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Biomedical subjects

L Cheng

Publications and source records attributed to L Cheng.

456 records · Page 26Linked to original sources
PubMedDate not supplied

Effect of estradiol on accelerated atherosclerosis in rabbit heterotopic aortic allografts.

Migration and proliferation of vascular smooth muscle cells are early and major events in the formation of atherosclerotic lesions. We report on an aorta transplant model in rabbits wherein myointimal proliferation is inhibited by 17-beta-estradiol. The abdominal aortas of outbred white New Zealand rabbits were harvested and allografted to the carotid artery of the recipient. The animals, which were fed either a normal or a high-cholesterol (0.5%) diet, were killed 3 weeks later. The degree of myointimal proliferation was measured with a digitized system attached to a light microscope. The myointimal hyperplasia was expressed as the cross section area of the intima/the area of the intima + the area of the media x 100. Transmission electron micrographs were obtained for all vessels. Intimal thickening was shown mainly to consist of proliferating smooth muscle cells. The cholesterol diet resulted in significantly higher serum total cholesterol levels compared to animals on a normal diet (p < 0.0001) but did not affect serum high-density lipoprotein-cholesterol or serum triglyceride levels. The cholesterol diet was also associated with a greater but not significant amount of intimal thickening. Treatment with 17-beta-estradiol significantly decreased both serum triglyceride concentration (p < 0.05) and myointimal thickening (p < 0.01) in cholesterol-fed animals. Transmission electron microscopy showed that the endothelial cells appeared structurally normal in the estradiol-treated animals. Further, estradiol prevented the appearance of vacuolized macrophages. Thus estradiol may decrease myointimal thickening by preserving the endothelium and preventing macrophage appearance in the intima.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMedDate not supplied

Evaluation of an antibody to human milk fat globule antigen in the detection of metastatic carcinoma in pleural, pericardial and peritoneal fluids.

An antibody to human milk fat globule-2 (HMFG-2) antigen was investigated to assess its value in detecting tumors in pleural, pericardial and peritoneal fluids. One hundred forty consecutive fluids were evaluated using the avidin-biotin complex (ABC) technique. Conventional cytology and HMFG-2-stained smears were compared using the former as the standard for tumor detection. Discrepant results were found in 15 specimens (8 false negatives and 7 false positives). Causes for discrepancy between the methods included lack of HMFG-2 antigen on tumor cells, random sampling error and endometrial cells ectopically located in the pelvic cavity or introduced during uterine instrumentation. We conclude staining for HMFG-2 may be a useful adjuvant technique for the detection of rare tumor cells in body fluids provided there is a high index of suspicion of metastasis from an HMFG-2-positive primary neoplasm.

Antibodies, Monoclonal↗
PubMedDate not supplied

Fine needle aspiration diagnosis of malignant lymphoma with confirmation by immunoperoxidase staining.

Cytospin preparations of fine needle aspirates in 14 cases of suspected lymphoma were studied by immunoperoxidase techniques. The combination of cytologic smears and immunoperoxidase studies resulted in a working diagnosis in 13 of the 14 cases. The immunologic markers in conjunction with the cytologic appearance of the aspirates were reliable and consistent in differentiating between malignant and benign lymphoproliferative lesions and in determining the B-cell or T-cell nature of the process.

Adult↗