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Biomedical subjects

L C Griel

Publications and source records attributed to L C Griel.

At least 37 records · Page 2Linked to original sources

Addition of sodium bicarbonate to complete pelleted diets fed to dairy calves.

During two trials, 35 and 27 Holstein calves were fed ad libitum complete, pelleted diets containing either 35% alfalfa (Trial 1) or 35% grass (Trial 2) hay from birth to 12 wk of age. Calves in Trial 1 were fed one of the following diets: control, control + 3.5% sodium chloride, or control + 5% sodium bicarbonate. In Trial 2, diets were: control, control + 5% sodium bicarbonate, or control + 5% sodium bicarbonate + loose, chopped grass hay. Intake of dry matter, gain in body weight, ruminal pH, or fecal starch did not differ. Calves fed sodium bicarbonate in Trial 1 but not 2 had a reduced feed efficiency compared with control and supplemented diets. In Trial 1 added sodium bicarbonate did not alter intake or digestible energy. Addition of sodium bicarbonate increased concentration of ruminal acetate and butyrate and decreased propionate in both trials. Fecal pH was elevated in calves fed sodium bicarbonate diets during both trials. Sodium chloride increased water intake in Trial 1, and sodium bicarbonate increased water indigestible energy. Addition of sodium bicarbonate increased concentration of ruminal acetate and butyrate and decreased propionate in both trials. Fecal pH was elevated in calves fed sodium bicarbonate diets during both trials. Sodium chloride increased water intake in Trial 1, and sodium bicarbonate increased water indigestible energy. Addition of sodium bicarbonate increased concentration of ruminal acetate and butyrate and decreased propionate in both trials. Fecal pH was elevated in calves fed sodium bicarbonate diets during both trials. Sodium chloride increased water intake in Trial 1, and sodium bicarbonate increased water intake in Trial 2. Incidence of free-gas bloat was higher in calves fed sodium bicarbonate in both trials. Addition of sodium bicarbonate to complete pelleted diets containing 35% alfalfa or 35% grass hay appeared to have no benefit for young, growing dairy calves in performance and health.

Animal Feed↗

Correlation between antler and long bone relative bone mass and circulating androgens in white-tailed deer (Odocoileus virginianus).

The relationship between serum androgen concentrations and relative density changes in the antlers and long bones of male deer was determined. Four male white-tailed deer were sampled 2 times a week during the antler-growing period. Serum androgen values were determined by radioimmunoassay, and relative bone mass (RBM) coefficients were determined by radiograph densitometry. As circulating androgen concentrations increased over the antler-growing period, the RBM coefficients of the antler increased from a mean of 18.5 (+/- 1.96 SD) to a mean of 57.7 (+/- 2.74) at 2 weeks after "rubout." Concurrently, the RBM coefficients of the metacarpus decreased from a mean of 52.0 (+/- 1.92) to a mean of 46.4 (+/- 1.86). There were positive correlation between increasing androgen concentrations and increasing antler RBM and negative correlation between androgens and decreasing metacarpus RBM. Antler RBM coefficients continued to increase after rubout, but metacarpus RBM did not change after rubout. Two castrated deer were injected subcutaneously with 1 g of testosterone and sampled every other day. Similar but smaller changes occurred in RBM values of the metacarpus and developing antler.

Androgens↗

Changes in portal blood metabolites and insulin with feeding steers twice daily.

Four Holstein steers surgically prepared with a device for sampling from the portal vein were used to examine changes in concentrations of metabolites and insulin in portal blood with scheduled meals. The animals were fed a complete mixed ration (3.4 kcal/g digestible energy) twice daily at three intakes. Blood was sampled at 30, 15, 10, and 5 min before feeding and at 1, 3, 5, 10, 15, 30, 45, 60, and 120 min after feeding began. Average intakes of food at 30 min for the three intakes were 0 (control), 889 g (restricted), and 1425 g (fed). Packed cell volume increased rapidly within 15 min of initiation of the meal in fed steers, but the maximum change from pre-feeding was 6.6%. Insulin increased within 5 min with the restricted intake and at 15 min in fed steers. Portal volatile fatty acids and beta-hydroxybutyrate also increased within 15 min of initiation of the meal in fed steers, and glucose tended to decrease after feeding. Certain metabolites and insulin of portal bood changed rapidly with feeding. Additional studies are required to establish any role of these blood components in the short-term control of feed intake in steers consuming concentrate feeds.

Animal Feed↗

Serum hormones in the perinatal pig and the effect of exogenous insulin on blood sugars.

Growth hormone (GH), insulin, cortisol, triiodothyronine (T3) and thyroxine (T4) were determined in serum of fetal pigs at 90, 100, 110 and 114 days of gestation and of newborn pigs at 1 and 24 hours of age. Fetal GH, T3 and cortisol increased between 90 and 110 days. GH concentration decreased between 110 and 114 days, while T3 and cortisol were unchanged. T4 was variable in fetal pigs and no relationship with T3 levels was apparent. Fetal insulin was constant at approximately 10 muU/ml. In newborn pigs there was a 3-fold and 2-fold increase in T3 and T4, respectively, during the first 24 hours of life. Cortisol in serum more than doubled during the first hour. Between 1 and 24 hours, there was a simultaneous decrease in cortisol and a 6-fold increase in insulin. No significant change in GH was noted during this time. Intraperitoneal insulin injected one hour before sacrifice induced a hypoglycemic response in newborn pigs, but not in fetal pigs.

Animals↗

Clinical and clinico-pathological effects of Escherichia coli endotoxin in mature cattle.

The effects of intravenous administration of Escherichia coli endotoxin were studied in eight mature lactating cows. Three cows were studied following intrammary infection with E. coli. Significant clinical findings are presented. Significant clinico-pathological findings include leukopenia, decreased blood serum calcium concentrations and increased levels of serum glutamic-oxaloacetic transaminase and serum ornithine-carbamyl transferase. Significant elevations of plasma corticosteroids were also noted.

Adrenal Cortex Hormones↗