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Biomedical subjects

L Buttifant

Publications and source records attributed to L Buttifant.

3 recordsLinked to original sources
PubMed1986

Sensitivity of veiled (dendritic) cells to cyclosporine.

The effect of cyclosporine (CsA) on dendritic cell function was tested by pulsing rabbit veiled cells (VC, the "dendritic" cells from the afferent lymph) with the drug. This treatment inhibited their capacity to enhance proliferative responses of autologous peripheral blood lymphocytes to the mitogen concanavalin A. The concentration of CsA required to produce inhibition varied from one rabbit to another and ranged from 5 to 100 ng/ml. The degree of inhibition was greater under conditions in which the normal VC were more effective. Addition of further untreated VC restored the response, but this restoration was incomplete under some culture conditions. Pulsing peripheral blood mononuclear cells (PBM) with CsA also produced a reduction in the stimulation by Con A, and the response was not restored by addition of normal VC. The experiments did not distinguish whether the effect of CsA was directly on dendritic cell function or via a secondary effect of "presentation" of CsA to lymphocytes by the dendritic cells.

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PubMed1982

Role of veiled cells in lymphocyte activation.

Peripheral blood lymphocytes from normal rabbits or from rabbits hyperimmunized with human immunoglobulin were cultured in 20-microliters hanging droplets. The cultures were stimulated with concanavalin A or, in the case of cells from sensitized animals, with human immunoglobulin. The addition to the cultures of small numbers of autologous or allogeneic veiled cells separated from afferent lymph increased the responses to low doses of the stimulants, particularly when the cells were cultured at low cell densities or for short periods of time. At high cell densities or at longer periods of culture, stimulation occurred in the absence of added veiled cells and was associated with clumping of the lymphoid cells and development of cells which morphologically resembled veiled cells found in afferent lymph. The enhanced responses upon addition of small numbers of veiled cells were also correlated with the formation of lymphoid aggregates which were frequently held together by the elongated processes of a single veiled cell. The observations support the view that the veiled cells are lymph-borne precursors of dendritic cells in the lymph nodes. This may provide an in vitro model for the cellular relationships which occur in paracortical cords of lymph nodes following antigenic stimulation.

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