Search PubMed⌕ Search

Biomedical subjects

L Beaudet

Publications and source records attributed to L Beaudet.

24 records · Page 2Linked to original sources
PubMed1988

Sequence and structure of the mouse gene coding for the largest neurofilament subunit.

We have determined the complete nucleotide sequence of the mouse gene encoding the neurofilament NF-H protein. The C-terminal domain of NF-H is very rich in charged amino acids (aa) and contains a 3-aa sequence, Lys-Ser-Pro, that is repeated 51 times within a stretch of 368 aa. The location of this serine-rich repeat in the phosphorylated domain of NF-H indicates that it represents the major protein kinase recognition site. The nfh gene shares two common intron positions with the nfl and nfm genes, but has an additional intron that occurs at a location equivalent to one of the introns in non-neuronal intermediate filament-coding genes. This additional nfh intron may have been acquired via duplication of a primordial intermediate filament gene.

Amino Acid Sequence↗
PubMed1987

Expression and assembly of a human neurofilament protein in transgenic mice provide a novel neuronal marking system.

To investigate the regulation of neurofilament gene expression, we have generated several lines of transgenic mice carrying multiple copies of a cloned human neurofilament (NF-L) gene. We show that a 21.5-kb DNA fragment including the human NF-L gene contains essential information for correct expression in nervous tissue of transgenic mice. The integrated genes are arranged in multiple tandem arrays, but the extent of transgene expression does not correlate with copy number nor does it influence the expression of the endogenous neurofilament genes. However, the levels of human NF-L protein recovered in neurofilament preparations from brains of transgenic mice correlate directly with the relative abundance of human NF-L mRNA detected in each line. There is an apparent delay in the accumulation of human NF-L protein during development, as determined by immunoblotting with a human-specific monoclonal antibody. Finally, immunohistochemical localization of the human NF-L protein results in the specific staining of neurons and their processes in transgenic mice.

Age Factors↗
PubMedDate not supplied

A baby has died: the impact of perinatal loss on family social networks.

OBJECTIVES: To describe the impact of a baby's death on the family's social network and to design nursing interventions to support families and their networks. DESIGN: Descriptive, with a qualitative approach. SETTING: An urban area of western Quebec. PARTICIPANTS: Twenty parents (mothers and fathers) who had experienced a perinatal loss (abortion, miscarriage, in-utero death, stillbirth, or death of a newborn within the 1st week of life) within the last 6 years. MAIN OUTCOME MEASURES: Self-administered questionnaires developed by the authors were completed by each parent. RESULTS: Family members' quality and quantity of ties with their network were profoundly affected by the perinatal loss. Some families experienced reinforcement of their bonds with their social network, but most suffered permanent losses of relationships with friends, colleagues, or extended family members. CONCLUSIONS: The quality and quantity of ties with one's network are associated with improved health status and life satisfaction. Considering the changes participants noted in their relationships within their network, further studies of the impact of these changes on family members' grieving process would be useful to guide nursing interventions.

Adaptation, Psychological↗
PubMedDate not supplied

Factors related to nursing burnout: a review of empirical knowledge.

It has been observed that nurses are at a high risk of burnout. The initiator variables of burnout appear to be numerous, tenacious, and not isolated; burnout is a complex phenomenon with multiple dimensions. In order to prevent psychosocial dysfunction and promote the mental health of nurses, the predominant factors contributing to nursing burnout should be clear. This article presents a literature review of the existing empirical knowledge regarding factors related to burnout in nurses. Among 300 documents on nursing burnout, 36 pertinent studies were retained. Amid these studies, 15 variables were selected, classified, and critically analyzed. It appears that the best correlates of nursing burnout are role ambiguity, workload, age, hardiness, active coping, and social support. Probable relationships and influences among these factors are discussed and may lead to a better understanding of mutual interactions between the personal and environmental factors contributing to nursing burnout. Implications for practice and further research are proposed.

Adaptation, Psychological↗