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Biomedical subjects

L Baum

Publications and source records attributed to L Baum.

At least 55 records · Page 3Linked to original sources
PubMed1992

Casein kinase II is associated with neurofibrillary tangles but is not an intrinsic component of paired helical filaments.

Neurofibrillary tangles (NFT) are pathological cytoskeletal structures composed of paired helical filaments (PHF), and are found in neurons of patients afflicted with many neurodegenerative disorders, including Alzheimer's disease (AD). We previously found that an antiserum against casein kinase II (CK-II) stained NFT intensely in the brain tissue of AD patients. In the current study, we found that the anti-CK-II antiserum stains NFT and neuronal inclusions in many other neurodegenerative diseases as well, including Guam-Parkinson dementia complex, chromosome 18 deletion syndrome, progressive supranuclear palsy, Kufs' disease, and Pick's disease. This antiserum reacted, in crude brain homogenates, with both a doublet of Mr 43,000 and a Mr 27,000 Da protein which could correspond to the alpha, alpha', and beta chains of CK-II. The staining of these bands was adsorbed by preincubating anti-CK-II antiserum with purified CK-II. Preincubation of brain sections with purified CK-II strongly intensified the immunostaining of NFT with anti-CK-II, suggesting that NFT may bind CK-II. In the AD brain homogenates, the particulate CK-II levels are increased whereas the cytosolic levels are decreased without a change in total CK-II levels, consistent with the idea that CK-II binds to the particulate PHF, a major constituent of NFT. In accord with these findings, purified PHF bound CK-II, but purified PHF did not contain CK-II as its component. These results suggest that CK-II might be an extraneously deposited component of NFT. Thus, the altered CK-II compartmentalization might have significant consequences in the pathogenesis of AD.

Brain↗
PubMed1991

Marginal accuracy of complete crowns made from alternative casting alloys.

The marginal accuracy of complete cast crowns made from five alternative casting alloys was measured and compared with that of crowns made from the traditional type III high-gold alloy. The alternative alloys studied were low-gold-palladium, high palladium, silver-palladium, nickel-chrome-molybdenum, and copper-aluminum. The findings indicated that Ag-Pd alloy crowns exhibited the best marginal accuracy among the alternative alloys tested. However, their marginal discrepancy value was still slightly higher than that of crowns made of type III high-gold alloy. Conversely, Ni-Cr-Mo alloy crowns exhibited the poorest marginal accuracy.

Cementation↗
PubMed1989

Flow cytometric analysis of lymphocyte subsets in peripheral blood of chronic headache patients.

Lymphocyte subsets from 5 groups of patients with different types of chronic headaches were analyzed by flow cytometry to determine the number of cells bearing the following markers: T11 (E-rosette receptor), T3 (total T cells), T4 (T-helper/inducer cells), T8 (T-cytotoxic/suppressor cells), B1 (B-cells), NKH-1 (Natural killer cells), I2 (Class II histocompatibility antigens, monomorphic determinants) and Mo2 (monocytes). In 4 of 5 of these groups, the absolute number of T8 lymphocytes was significantly lower than that of control group. In addition, the T4:T8 ratio was higher in these 4 groups. These findings may suggest a relationship between the immune system and the cause of chronic headaches.

Antibodies, Monoclonal↗
PubMed1987

Intestinal myiasis in a baby attending a public health clinic.

This article describes a case of intestinal myiasis--the presence of fly larvae in the intestines--in a 12-month-old baby. The asymptomatic child was twice treated by her physician for a presumptive diagnosis of pinworm infection. The mother continued to see "worms" in the child's stool and brought her to a public health primary care clinic where she was evaluated by nurse practitioners. Larvae (maggots) of the false stable fly, Muscina stabulans, were identified in each of two stool specimens collected on different days. Examination of stool specimens from other family members showed no larvae. The likely source of the child's infection was over-ripened bananas, which were kept in a hanging basket. No pharmacologic treatment was prescribed, but the parents were instructed to cover the fruit and wash it before consumption. Nurse practitioners are encouraged to report suspected cases of myiasis and to work with patients, their families and public health personnel in order to confirm the diagnosis, identify the source and make suggestions to prevent further infestation.

Ambulatory Care Facilities↗
PubMed1985

Retentive pins. Their use and misuse.

Retentive pins have brought with them both ecstasy and agony. Their history, use, and situations resulting from their misuse are reviewed in this article. Correction of common errors will prove useful to the reader.

Dental Amalgam↗
PubMed1984

Gold foil.

Explore the source record for details and available documents.

Dental Cavity Preparation↗
PubMed1983

Cardiovascular toxicity and tissue proton T1 response to manganese injection in the dog and rabbit.

Paramagnetic substances that shorten proton T1 relaxation time have promise as nuclear magnetic resonance (NMR) contrast agents. The cardiovascular toxicity and tissue T1 response to 0.01-0.1 mM/kg intravenous doses of MnCl2 in rabbits and dogs were studied. There were dose-dependent changes in cardiac electrophysiology and arterial blood pressure consistent with the calcium-blocking properties of manganous ion. There were also dose-dependent and organ-specific changes in tissue proton T1. The sensitivity of organ T1 was expressed as the dose of manganese required to shorten the normal T1 by 50%. Liver, kidney, and heart were most sensitive, while skeletal muscle and adipose tissue were insensitive. MnCl2 is an effective NMR contrast agent, but its toxicity may be excessive for clinical use.

Animals↗
PubMed1983

C-reactive protein antigenicity on the surface of human lymphocytes.

We reported previously that C-reactive protein (CRP), when complexed to a multivalent binding specificity, can bind to a subset of lymphocytes that bear the IgG Fc receptor. Recently we showed that anti-CRP plus complement depletes natural killer function. We now show the detection of CRP on the surface (S-CRP) of a small percentage of PBL and on a minor population of phagocytic mononuclear cells. S-CRP is not detectable by direct immunofluorescence, but is readily discernible by using more sensitive techniques such as biotinylated anti-CRP with fluorescent avidin or indirect immunofluorescence with monoclonal anti-CRP. S-CRP is expressed in the absence of calcium, whereas the binding of complexed CRP is calcium dependent. S-CRP does not appear to be the exclusive binding site for CRP-CPS because anti-CRP does not block the binding of complexed CRP. Both the binding site for complexed CRP and S-CRP can be capped off; however, the kinetics of capping of these two surface molecules is different. Cells binding complexed CRP are OKT11-, whereas a significant number of PBL bearing S-CRP are OKT11+. Therefore cells bearing S-CRP appear to be a separate population from those that bind complexed CRP. These studies establish the presence of S-CRP on a subpopulation of lymphocytes, define conditions required for its detection, and speak to certain of the relationships between S-CRP and the binding site of CRP-CPS complexes.

Antibodies, Monoclonal↗