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Biomedical subjects

L Balducci

Publications and source records attributed to L Balducci.

At least 145 records · Page 8Linked to original sources
PubMed1980

Use of Hoechst 33258 fluorochrome for detection of mycoplasma contamination in cell cultures: development of a technique based on simultaneous fixation and staining.

A simple rapid technique for detection of mycoplasma contamination in cell cultures by a modified Hoechst 33258 staining procedure, is described. After brief alcoholic fixation in situ, cell monolayers were definitively fixed and simultaneously stained by the addition of a 0.001% (w/v) Hoechst 33258 fluorochrome solution in ethanol for a few minutes. This method and conventional mycoplasma isolation techniques resulted in comparable sensitivity. Mycoplasma contaminants were detected in 21 out of 217 cell culture specimens tested. However, two different specimens were positive only by either procedure. A sequential use of both techniques, based on the search for mycoplasma contamination by microbiological methods only in Hoechst 33258 - negative samples, should provide a reasonable degree of sensitivity, being also accessible to virtually every laboratory involved in tissue cuture work.

Benzimidazoles↗
PubMed1980

Rapid diagnosis of respiratory syncytial virus infections by immunofluorescence on cell smears from nasopharyngeal secretions.

The efficiency of indirect immunofluorescence for detection of respiratory syncytial (RS) virus antigens in nasopharyngeal secretions was compared with virus isolation on highly sensitive cell culture systems maintained in serum-free media. Twenty out of 22 (91%) culture-positive specimens were correctly identified by indirect immunofluorescence, which gave no false positive results. Since clinical specimens were sometimes unsuitable for immunofluorescence studies, this could explain two false negative results. Thus, this technique proved to be a reliable tool for early diagnosis of RS virus infections; however, in order to avoid false negative results, it should be combined with sensitive cell culture isolation systems.

Child, Preschool↗
PubMed1979

Septicemia during antibiotic therapy in neutropenic patients.

Two patients with acute leukemia developed septicemia while receiving antibiotic therapy. In both cases, the microorganism grown from the blood was sensitive to the drugs the patients were receiving. Areas of colonization by the same organisms were found at autopsy in the necrotic spleen and in the necrotic colonic mucosa. Septicemia developing during antibiotic therapy can indicate colonization of necrotic organs and appropriate diagnostic and therapeutic measures may be necessary.

Adult↗
PubMed1978

Acute megakaryocytic leukemia. Description of a case initially seen as preleukemia syndrome.

A 52-year-old man had aregenerative anemia unresponsive to pyridoxine hydrochloride. Acute leukemia developed, and he died four months after diagnosis. At autopsy he had acute megakaryocytic leukemia with involvement of bone marrow, liver, spleen, adrenals, kidneys, and thyroid. Chromosomal analysis revealed absence of both diploid and Ph1 chromosomes. A mode of 45 chromosomes and aneuploidy were present. This is similar to the only other case with chromosomal studies. Of the 15 acceptable documented cases, eight were men and seven were women. Their age varied from 28 to 76 (mean, 55) years. Only two were less than 40 years of age. Most had pancytopenia, and all were dead within six months of diagnosis.

Autopsy↗
PubMed1978

Erythroleukemia: in vitro studies of erythropoiesis.

We studied the growth of erythroid burst-forming units (BFU-E) and erythroid colony forming units (CFU-E) from bone marrow and blood in six patients with erythroleukemia. Five patients grew CFU-E, while BFU-E were found in the marrow of two and in the peripheral blood of only one patient. In all cases with colony growth, the numbers of colonies were markedly decreased with respect to normal controls. Patient BFU-E were composed of fewer clusters than those of controls. BFU-E and CFU-E growth was dependent on the addition of erythropoietin to the medium, and no growth was observed in absence of erythropoietin. At present it is not known if the growth obtained is derived from residual normal erythropoietic stem cells or from abnormal erythroid precursors of the leukemic cells.

Adult↗
PubMed1978

[Herpetic meningoencephalitis in infants. Study of 7 cases].

Seven children with herpes meningoencephalitis aged between 14 days and 13 months were studied. The herpes infection was confirmed by isolation of herpes virus from the CSF (one case), from the brain (one case), and in the 5 remaining cases by serology. The strains isolated were herpes type 2. The clinical features of herpes meningoencephalitis in the first year of life and the laboratory data are described.

Adult↗
PubMed1976

Intranasal versus subcutaneous rubella vaccination in schoolgirls.

In this study a venous blood specimen was drawn and, at the same time, rubella vaccine was given to 1906 schoolgirls mostly in the pre-pubertal age. To assess the pre-vaccination immune status and the effect of the vaccine, a second blood sample was taken 6 weeks later and tested for rubella HAI antibody simultaneously with the first one. RA 27/3 rubella vaccine administered by intranasal route to 81 girls produced a 100% seroconversion rate and the same vaccine strain, given by subcutaneous route to 460 girls, produced a 94.73% seroconversion rate. Among the 1, 365 schoolgirls who received subcutaneous Cendehill vaccine, the seroconversion rate was 86.68%. Side effects were mild and transient and occurred, as headache, most frequently among the girls who received RA 27/3 vaccine by intranasal route.

Administration, Intranasal↗