Search PubMed⌕ Search

Biomedical subjects

L Baird

Publications and source records attributed to L Baird.

31 records · Page 2Linked to original sources
PubMed1990

Identification, cloning, and characterization of the Escherichia coli sohA gene, a suppressor of the htrA (degP) null phenotype.

Two extragenic suppressors which allow temperature-sensitive htrA mutant Escherichia coli bacteria to grow at 42 degrees C and simultaneously acquire a cold-sensitive phenotype at 30 degrees C were isolated. The cold-sensitive phenotype exhibited by one of the mutants was used to clone the corresponding wild-type copy of the suppressor gene. This was done through complementation with a mini-mu plasmid E. coli DNA library, by selection for colonies which were no longer cold sensitive, at 30 degrees C. The cloned suppressor gene was shown to complement the cold-sensitive phenotype of both suppressor mutations. It was mapped to 68 min on the E. coli chromosome through hybridization to the Kohara library of overlapping lambda transducing bacteriophages, which covers the entire E. coli chromosome. The complementing gene was further subcloned on an 830-base-pair (bp) DNA fragment. DNA sequencing revealed the presence of an open reading frame (ORF) of 333 bp which could encode a protein of 12,359 Mr. Subcloning of various DNA fragments from within this 830-bp DNA fragment suggests that this ORF is most likely responsible for suppression of the cold-sensitive phenotype of the htrA suppressor bacteria. By using a T7 polymerase system to overproduce plasmid-encoded proteins, a protein of approximately 12,000 Mr was produced by this cloned DNA fragment. This ORF defines a previously undiscovered gene in E. coli, called sohA (suppressor of htrA).

Amino Acid Sequence↗
PubMed1989

Identification, characterization, and mapping of the Escherichia coli htrA gene, whose product is essential for bacterial growth only at elevated temperatures.

We identified and cloned an Escherichia coli gene called htrA (high temperature requirement). The htrA gene was originally discovered because mini-Tn10 transposon insertions in it allowed E. coli growth at 30 degrees C but prevented growth at elevated temperatures (above 42 degrees C). The htrA insertion mutants underwent a block in macromolecular synthesis and eventually lysed at the nonpermissive temperature. The htrA gene was located at approximately 3.7 min (between the fhuA and dapD loci) on the genetic map of E. coli and between 180 and 187.5 kilobases on the physical map. It coded for an unstable, 51-kilodalton protein which was processed by removal of an amino-terminal fragment, resulting in a stable, 48-kilodalton protein.

Chromosome Mapping↗
PubMed1984

Production and characterization of monoclonal antibodies to peripheral and central nervous system myelin.

Monoclonal antibodies against P0, myelin basic protein, or myelin-associated glycoprotein were generated by fusing mouse myeloma cells with spleen cells from BALB/c mice immunized with central and peripheral nervous system myelin proteins. The antibodies secreted were either IgG, IgM, or IgA. Clone C6B5 (iso-type IgM) secreted antibody(ies) that bound to both myelin basic protein and myelin-associated glycoprotein, although binding of antibody to myelin basic protein as detected by the immunoblot technique appeared to be much less than to the myelin-associated glycoprotein. Antibodies were characterized in solid-phase radioimmunoassay for their species cross-reaction, and histologically for the specificity of binding to myelin in central and peripheral nervous system tissues. These monoclonal reagents should prove valuable in studying CSF and myelin-producing cells, since in both cases the concentration of myelin proteins is low.

Animals↗
PubMed1984

Rapid cytological diagnosis of rectal cancer.

A simple, safe, rapid and accurate technique is described for cytological diagnosis of ulcerated or nodular rectal cancer done while performing digital rectal examination or sigmoidoscopy. In 21 cases, there were 17 true positives and no false positives for cancer. There were two true negatives and one false negative for rectal cancer. It is important to exclude prostatic cancer if, in the male, the lesion is anterior and situated at or near the level of the prostate gland, and this can be done using an immunoperoxidase technique which will identify cancer cells of prostatic origin.

Adenocarcinoma↗
PubMed1983

Career dynamics: managing the superior/subordinate relationship.

Building on research and writing in the fields of career management and mentor relationships, Baird and Kram analyze the superior-subordinate relationship as an exchange to which each party brings different needs and resources. They point out that this relationship can be productive and satisfying--both for the parties concerned and for the organization--when the needs of one party match the resources of the other. The article includes a checklist for analyzing how the superior-subordinate relationship operates as an exchange and how the resources of the parties mesh or fail to mesh. They do on to show how the superior-subordinate relationship and the needs of the parties change as each moves through individual career and life cycles. What was once a productive relationship may, in time, become unproductive, or vice versa. In any event, its dynamic nature requires that it be managed. Baird and Kram suggest five steps for managing the relationship as it moves through these changes: (1) Recognizing that the relationship is an exchange; (2) identifying clearly one's own as well as the other party's needs; (3) understanding how the subordinate's and boss's needs fit together and recognizing that the relationship is likely to change; (4) understanding the constraints under which the boss operates; (5) establishing a feedback and evaluation process for continuously assessing the relationship.

Administrative Personnel↗
PubMed1976

Effect of 1-methyl-1-nitrosourea and streptozotocin on glucose-induced insulin secretion by isolated islets of Langerhans.

The present experiments were designed to compare the effects of streptozotocin and 1-methyl-1-nitrosourea upon glucose-induced insulin secretion by isolated islets of Langerhans. Both drugs depressed the insulin response at one and two hours incubation but higher molar concentrations of the nitrosourea were required to produce the same level of inhibition as streptozotocin, a difference perhaps related to the latter's glucose moiety.

Animals↗
PubMedDate not supplied

Meshing human resources planning with strategic business planning: a model approach.

Many people are touting the need to take a strategic approach to human resources management--but this is more easily said than done, point out authors Lloyd Baird, associate professor of management at the School of Management and the Human Resources Policy Institute at Boston University; Ilan Meshoulam, personnel executive at Digital Equipment Corporation; and Ghislaine DeGive, Boston University. Obviously, people cannot be moved around as easily or as speedily as can other resources to help move a company to a strategically identified future position. Thus a great deal of planning is required. The authors present an integrative human resources strategic planning model that focuses on four elements: the environment, the organization's culture, the corporate mission statement, and overall corporate strategy. Each part of the model is thoroughly discussed, and checklist questions are included to help the individual manager develop and implement human resources management plans for his or her own organization.

Models, Theoretical↗
PubMedDate not supplied

Managing human resource strategies. A five-stage model of growth and development.

To summarize, our model can be used to strategically manage human resources. It provides a framework first to assess the current position of human resource management in relation to each one of the strategic components. It then provides the framework for assessing where each one of the components should be in relation to the business needs. Closing the gaps on the dual fit will provide the function with a strategic plan to identify opportunities and risks in the business environment. The model can be used to analyze any unit or level in an organization. The model provides a framework for understanding the development of the HRM function in the organization. Learning from previous errors will most likely prevent new ones.

Laboratories, Hospital↗
PubMedDate not supplied

Synthesis and biological activity of novel, potent and long-acting analogs of AC-CCK-7 with high affinity for peripheral (type A) receptors.

Analogs of cholecystokinin (CCK-7) in which the N-terminal Tyr(SO3H) was acetylated, Asp6 replaced with Thr(SO3H) and Phe7 replaced with N-methyl-Phe were prepared by solid-phase peptide synthesis and evaluated for their receptor-binding activity and ability to suppress appetite. The receptor binding activities of these synthetic analogs of CCK-7 and their selectivity for the CCK-A and CCK-B receptor subtypes were determined using solubilized membrane preparations from rat pancreatic tissue and bovine striatum. The synthetic peptide Ac-Tyr(SO3H)-Met-Gly-Trp-Met-Thr(SO3H)-N-methyl-Phe-NH2 (referred to as Ro 23-7014) demonstrated superior satiating potency (ED50 = 0.3 micrograms/kg, i.p.), increased selectivity for CCK-A receptors (400-fold), increased resistance to peptidergic degradation and a longer duration of action (4 to 5 hours). This analog also effectively suppressed food intake following intranasal administration (ED50 = 100 micrograms/kg). These studies demonstrate the feasibility of designing analogs of CCK-8 with greater selectivity, potency and duration of action, which may be useful as nonsystemically administered appetite suppressants.

Acetylation↗