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Biomedical subjects

L B Wilson

Publications and source records attributed to L B Wilson.

At least 73 records · Page 4Linked to original sources
PubMed1982

Meeting the mental health needs of the aged: the role of psychiatric emergency services.

The mental health emergency service is a critical triage point for making decisions about the delivery of a variety of services needed by an increasingly large elderly population. This study explores the roles of two mental health emergency services in providing services and linking elderly clients with elements of the health, mental health, and long-term care systems. A data base was established by a retrospective review of 350 records of elderly clients treated at two urban mental health emergency facilities over a one-year period. Key findings are presented about the demographic characteristics of users age 60 and over, diagnoses, referral and disposition patterns, repeater usage, and emergency versus nonemergency usage. Recommendations are made on the future role of mental health emergency services in meeting the complex health and mental health needs of the elderly in the community.

Aged↗
PubMed1982

Creating comprehensive gero-psychiatric care in the community.

The aged increasingly need a comprehensive system, a central access point, and coordinated care in order for their acute and chronic, health and mental health needs to be met. This research reviews and analyzes the structure, function, and operation of a model demonstration geriatric emergency service which functioned as an access point and coordinator of care for the elderly in a network of health, mental health, and long term care services. The findings of this study indicate the important contribution a geriatric emergency service can make to creating comprehensive gero-psychiatric care in the community.

Aged↗
PubMed1981

Direct identification of sickle cell anemia by blot hybridization.

Several reports have been published on the use of polymorphisms found in the human hemoglobin genes as a means for prenatal diagnosis of sickle cell anemia. The disadvantages of this approach reside in its limited application and the need for family analysis. Here we report that, by use of restriction endonuclease Dde I and diazobenzyloxymethyl-paper transfer procedures, a direct analysis can be made. Individuals with normal hemoglobin (AA) show two bands (175 and 201 base pairs) complementary to a 5'-specific beta-globin gene probe. Sickle cell trait individuals (AS) exhibit an additional band (376 base pairs). Individuals with sickle cell anemia (SS) show the band at 376 base pairs with a concomitant loss of the 175-base pair band. We interpret these changes in banding pattern to be the result of the elimination of a restriction site for Dde I in the altered codon associated with the sickle cell allele. Because an analysis can be performed on as little as 20 micrograms of cellular DNA, the application to prenatal diagnosis of sickle cell anemia should be possible.

Anemia, Sickle Cell↗
PubMed1980

Nucleotide sequence of the coding portion of human alpha globin messenger RNA.

The nucleotide sequence of the coding portion of human alpha globin mRNA has been determined by sequence analysis using human alpha globin cDNA cloned in bacterial plasmids. The sequence was obtained by a combination of direct sequence analysis of the cloned cDNA and analysis of cDNA obtained by primer extension, using short restriction endonuclease fragments of cloned alpha cDNA that were hybridized to human globin mRNA and elongated on the mRNA template by viral reverse transcriptase. The human alpha globin mRNA has an unexpectedly high G + C base composition (64.7%), similar to that observed for rabbit globin alpha mRNA, and displays a striking bias in the use of synonym codons for various amino acids. The bias in codon usage of human alpha globin mRNA is similar, with some exceptions, to that previously observed for rabbit alpha globin mRNA as well as for human and rabbit beta globin mRNAs. A detailed restriction endonuclease map of the human alpha globin cDNA is presented.

Amino Acid Sequence↗
PubMed1980

Community mental health centers and the elderly: a time for expansion of planning, research, and demonstration projects.

The purpose of this paper is to acquaint persons working in Community Mental Health Centers with federal funding resources which support planning, research, and demonstration projects related to mental health and aging. The significance of exploration by CMHCs into the aging area is suggested by the legislative histories of CMHCs and aging programs, our expanding aging population which requires increasing health and mental health services, and the growing number of resources available for the development of services related to aging. Principal funding sources related to mental health and aging are: Alcohol, Drug Abuse and Mental Health Administration, National Institutes of Health; Administration on Aging; and Health Care Financing Administration. Each funding source is presented through a review of five key items: 1) name of program; 2) objectives; 3) funds awarded; 4) legislative mandate; and 5) contact person and address. Program areas for each funding source are discussed. Knowledge of these funding resources should enable CMHCs to plan services and to expand capabilities to serve the growing over-60 population.

Aged↗
PubMed1980

Nucleotide sequence of human G gamma globin messenger RNA.

The nucleotide sequences of the entire coding portion and 3'-untranslated portion of the human G gamma globin mRNA have been determined by direct nucleotide sequence analysis of cloned human G gamma globin cDNAs. These nucleotides sequences have been compared to the previously published sequences of the human beta globin mRNA and A gamma globin mRNA. No differences were detected between the coding sequences of G gamma and A gamma globin mRNAs except for the expected difference at codon No. 136, but a number of differences were detected in the 3'-untranslated sequences of the two mRNAs. Codon usage of G gamma globin mRNA, although similar to that of the related beta globin mRNA, displays a few notable differences such as the use of UUG for leucine and GGA for glycine that are not utilized in beta globin mRNA.

Base Sequence↗
PubMed1980

Gene structure in hereditary persistence of fetal hemoglobin individuals.

Gel blot hybridization studies on DNA from individuals heterozygous for different types of hereditary persistence of fetal hemoglobin (HPFH) have shown a good correlation in gene structures with those deduced from data obtained in studies of the hemoglobins found in members of the families. The recent data have also shown that in certain individuals additional strong hybridizing bands are present. The origin of these bands is as present unresolved, but it may be that they represent additional genes.

DNA↗
PubMed1979

Detection of proteins like human gamma and beta globins in Escherichia coli carrying recombinant DNA plasmids.

Escherichia coli strain chi 1776 carrying recombinant DNA plasmids containing cDNA copies of human beta or gamma globin mRNAs has been shown by radioimmunoassay to synthesize polypeptides antigenically related to the beta and gamma chains of human hemoglobin. The gamma and beta polypeptides have been enriched from lysates on immunoabsorbent columns containing hemoglobin antibodies and shown to specifically inhibit the antigen-antibody binding between 125I-labeled hemoglobin and the homologous antibody but not other hemoglobin-antihemoglobin reactions. Clone JW151, which is known to contain a complete copy of the coding portion of the gamma globin mRNA, has been shown to produce a protein that reacts specifically with antibody to the chain of fetal hemoglobin, hemoglobin Kenya, and hemoglobin Bart's.

Base Sequence↗
PubMed1978

Insertion of synthetic copies of human globin genes into bacterial plasmids.

Double stranded human globin cDNA was synthesized by use of viral reverse transcriptase from globin mRNA of cord blood of premature infants requiring exchange transfusions. The cDNA was introduced into plasmids and the recombinant DNA plasmids used to transform E. coli X1776. A number of transformants were obtained. Plasmid DNA from selected colonies was isolated and characterized for the type of globin cDNA it contained by three types of procedures: 1) hybridization to previously characterized 3H-labeled alpha,beta and gamma cDNA; 2) analysis of the size and nature of fragments produced by digestion of the plasma DNA by different restriction endonucleases; and 3) by rapid DNA sequence analysis of selected DNA fragments produced by restriction endonuclease digestion. Analysis by these techniques of plasmid DNA from different colonies has definitively identified the presence of human alpha, beta or gamma cDNA sequences in different plasmids.

Base Sequence↗
PubMed1977

Human globin messenger RNA: importance of cloning for structural analysis.

The sequence of most of the human beta globin messenger RNA and large sections of the alpha globin messenger RNA has been determined. Partly because of genetic polymorphism, it was necessary to clone globin complementary DNA in order to extend the analysis. Purified human fetal globin messenger RNA was isolated and used as a template by reverse transcriptase to produce duplex complementary DNA molecules. These molecules were linked in vitro to plasmid DNA by use of T4 ligase in the presence of Escherichia coli Pol 1. Several colonies transformed by these molecules have been shown to hybridize with labeled human globin complementary RNA.

Base Sequence↗
PubMedDate not supplied

Cardiorespiratory and phrenic nerve responses to graded muscle stretch in anesthetized cats.

This study examined the cardiovascular, ventilatory, and phrenic nerve responses to graded activation of mechanically sensitive muscle afferents. Using eight alpha-chloralose anesthetized cats, the left and right triceps surae muscles were stretched individually and simultaneously at progressive increments (0.5, 1.0, 1.5, 1.75 cm). Muscle stretch elicited sustained increases in mean arterial blood pressure (MAP) and heart rate (HR). These changes were related to the degree of stretch, as stretching one muscle 0.5 cm increased MAP 15 +/- 2 mmHg and HR 7 +/- 2 beats/min, while stretching both legs (1.75 cm) increases these variables 40 +/- 11 mmHg and 11 +/- 3 beats/min. By contrast, muscle stretch initially decreased ventilation and phrenic nerve activity. After the initial fall, ventilation, but not tidal phrenic activity, increased above baseline. These results show that a divergence exists between the initial cardiovascular and ventilatory responses to activation of mechanically sensitive muscle afferents. Further, the hyperpnea elicited by muscle stretch in spontaneously breathing anesthetized cats appears to be the result of excitation of non-diaphragmatic muscles of ventilation.

Animals↗