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Biomedical subjects

L B Taichman

Publications and source records attributed to L B Taichman.

59 records · Page 4Linked to original sources
PubMed1980

Misconceptions about dental treatment for patients with genetic disease.

It is the goal of this conference to define the role of dentistry to the interdisciplinary treatment of genetic diseases. Certainly, with the inclusion of dental disciplines in centers devoted to the diagnosis and treatment of genetic disease, the diagnosis and treatment of secondary craniofacial manifestation and the diagnosis and treatment of genetic disease primary to the craniofacial and oral complex will be greatly accelerated and the many misconceptions about dental treatment for patients with any genetic disease will be significantly reduced.

Dental Care↗
PubMed1979

The use of ultraviolet light in the fractionation of chromatin containing unsubstituted and bromodeoxyuridine-substituted DNA.

Two procedures are described for the fractionation of chromatin containing unsubstituted (LL) DNA and DNA unifilarly substituted with bromodeoxyuridine (HL). The two procedures rely upon the sensitivity of bromodeoxyuridine-containing DNA to UV light to induce either strand breakage or protein crosslinking. When a mixture of LL and HL chromatin is irradiated with UV light, the HL DNA fragments into molecules of smaller molecular weight than the LL DNA and crosslinks more chromosomal protein than the LL DNA. LL and HL chromatin can be fractionated on the basis of size by centrifuging through a neutral sucrose gradient. The HL DNA-protein adducts that are generated by the UV light have a unique buoyant density and may be isolated by isopycnic centrifugation in CS2SO4. The ability to fractionate LL and HL chromatin permits certain studies on the structure of replicating chromatin.

Animals↗
PubMed1979

Repair of ultraviolet light damage to the DNA of cultured human epidermal keratinocytes and fibroblasts.

Pure cultures of dermal fibroblasts and epidermal keratinocytes have been obtained from a single biopsy of newborn foreskin. The cells were labeled, exposed to several doses of UV light, and allowed to repair in the dark for 16 hr. The number of pyrimidine dimers before and after repair was assessed by measuring the numbers of sites in the DNA sensitive to a specific UV endonuclease. At all doses used, the extent of repair was similar in the cultured keratinocytes and cultured fibroblasts.

Cells, Cultured↗
PubMedDate not supplied

Systemic replacement therapy from genetically modified epidermal keratinocytes.

Epidermal keratinocytes are a potential vehicle for gene transfer and systemic delivery. We review data showing that epidermis-secreted protein does indeed reach the circulation, and we discuss factors that bear upon the issue of how much protein epidermal keratinocytes can deliver to the circulation.

Animals↗