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L B El'bert

Publications and source records attributed to L B El'bert.

34 records · Page 2Linked to original sources
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[Antigenic activity of a concentrated and purified cultured antirabies vaccine from the Vnukovo-32-107 strain].

Humoral response (virus-neutralizing antibody production) to immunization with inactivated 30-fold concentrated and purified tissue culture rabies vaccine was studied in monkeys and human volunteers. Two doses of the preparation spaced 20 days apart and given intramuscularly or intradermally to monkeys induced in them much more intensive antibody production (4--213-fold by titer) than 6 daily injections of unconcentrated vaccine. In 10 human volunteers immunized with 1.5 ml doses of the concentrated vaccine intramuscularly twice 20 days apart antibody titers 40 days after immunization varied from 1:79 to 1:6250 (average 1:1067). In another group of 10 human volunteers immunized intramuscularly with the concentrated vaccine 3 times at 0, 1st and 20th day, antibody titers 40 days after immunization were 1:112--1:3273 (average 1:508). These values are significantly higher than the average titers in humans vaccinated with unconcentrated tissue culture of brain vaccine of Fermi type. The concentrated vaccine proved to be a preparation of low reactogenicity: no systemic reactions were observed among vaccinated volunteers; 4 out of 20 volunteers had a mild, transient (1 day) local reaction (hyperemia of less than 1.5 cm in diameter).

Animals↗
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[Gel filtration chromatography of tick-borne encephalitis virus on macropore glass].

The capacity of some chemically treated porous glass to adsorb tissue culture virus of tick-borne encephalitis was studied. Chemical binding of albumin and succinanhydride with the porous glass matrix was found to eliminate virion adsorption on the carrier surface. This permitted to carry out gel filtration chromatography of several virus strains and to obtain highly purified preparations of tick-borne encephalitis virus. Gel filtration on modified porous glass with different pore sizes was used for the determinations of cromatographic radius of virus particle.

Chromatography, Gel↗
PubMedDate not supplied

[Immunity indices of mice immunized with different vaccines against Japanese encephalitis].

The test of adoptive transfer of immunity demonstrated that in the mice vaccinated with inactivated Japanese encephalitis vaccines the protective activity to the homologous virus is provided predominantly by B-lymphocytes the protective effect of which is enhanced by cooperative interaction with T-lymphocytes. The culture (CV) and purified brain (BV) vaccines induce high levels of humoral and cell-mediated immunity, however, the capacity of CV to produce antibody is higher than that of BV. Both preparations do not cause in vaccinated mice any changes in responsiveness to extraneous antigen (sheep red cells), or disorders in chemotaxic mobility of leukocytes and phagocytic activity of macrophages.

Animals↗
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[Inactivated vaccine against Japanese encephalitis. The characteristics of a purified preparation from mouse brain].

A vaccine has been prepared from formalin-inactivated suspension of brain tissue of Japanese encephalitis (JE) virus-infected white mice. The material was purified by flow ultracentrifugation in sucrose density gradient. Four members of the Nakayama antigenic group were compared as production JE virus strains for the vaccine preparation. Satisfactory results with regard to the immunogenicity of the bulk vaccine determined in mice (the intensity of response and the antigenic spectrum range) were obtained for three of them, including only one of the two variants of the Nakayama NIH strain. The highly purified experimental vaccine proved to be immunologically active and safe in trials in laboratory animals and 20 human volunteers. After two vaccinations, immunological changes in people were recorded much more frequently in the neutralization test than in the hemagglutination-inhibition test. Blasttransformation of lymphocytes in response to the specific antigen was observed in 5 out of 7 vaccines examined. There was no strict correlation of this test with the results of both serological tests.

Adult↗
PubMedDate not supplied

[Donor immunization with an inactivated concentrated purified vaccine against tick-borne encephalitis to obtain immune blood preparations].

Vaccination of seronegative and seropositive donors with a concentrated purified vaccine (CV) against tick-borne encephalitis induced antibody synthesis in a concentration sufficient for preparation of donor immunoglobulin with antibody titres of 1:320 to 1:1280 by HI test. The resulting preparation showed a high antiviral activity both in vivo and in vitro against antigenically different strains of tick-borne encephalitis virus. The immune plasma derived from CV-immunized donors had a therapeutic effect in patients with tick-borne encephalitis. The optimal schedules for immunization of donors are discussed.

Adult↗
PubMedDate not supplied

[Reactogenicity and immunological effectiveness of a concentrated, purified vaccine against tick-borne encephalitis].

Human reactogenicity and immunological efficacy of concentrated purified tick-borne encephalitis (CTBEV) vaccine was studied in a controlled trial. The new vaccine was found to be superior to the commercial preparation in its capacity to induce specific humoral and cell-mediated immunity in man and in reduced sensitizing activity with regard to nonviral cellular antigens. Two vaccinations with CTBEV in doses of 0.5 ml each at 6-month interval are recommended. At this vaccination schedule virus neutralizing antibodies were detectable in 95% vaccines. Inoculation of 0.5 ml CTBEV is accompanied by fever of mild and moderate intensity in 19% and 3% of the vaccines, respectively. In this respect CTBEV is less reactogenic than the commercial preparation.

Adolescent↗
PubMedDate not supplied

[Cultivation of monolayer cultures of primary and transplantable animal cells in a device with regulated growth conditions].

Primary, diploid and continuous cell lines were cultivated in the apparatus "Cellotron-1". Rashig's ring was used as an underlay in the apparatus. Fresh medium perfusion, pH control, the medium aeration, temperature control, as well as dissolved oxygen control were exercised in the cultivator. Various cell types have demonstrated a significant increase as compared to the control stationary cultivation: diploid green monkey embryo cells increased 7.5-fold for 5 days, continuous green monkey kidney cells 8-fold for 4 days, primary monkey kidney cells 2-fold for 7 days.

Animals↗
PubMedDate not supplied

[Immunological status of people inoculated with various types of inactivated vaccine against tick-borne encephalitis].

As compared with cellular immunity tests, serological tests more frequently detect virus-specific immune response in humans immunized with concentrated purified vaccine (CV) against tick-borne encephalitis. Combined determination of cell reactions and antibody production after a single injection of the vaccine increases the rate of detection of specifically responding persons. The duration of the inactivated vaccine-induced immunity is determined not only by the level of serum antibodies but also by the state of immunological memory detectable by the capacity of the host to produce antibodies after another injection of the specific antigen. At 14 and 30 days after a single immunization with CV IgG antibody is found in the blood of the vaccinees. In the course of 3 vaccinations, the concentration of serum immunoglobulins, chemotaxic activity of leukocytes, and blood lymphocyte reactivity to mitogens do not change. The role of some immunological values as criteria for the evaluation of the effectiveness of vaccine purification from impurities is discussed.

Adolescent↗
PubMedDate not supplied

[Chromatography of formalin-inactivated tick-borne encephalitis viruses on macroporous glass].

The results of further development of methods for chromatographic concentration and purification of tick-borne encephalitis virus on columns with porous glass using formalin-inactivated virus suspensions are presented. The method of adsorption chromatography on porous glass under optimal conditions concentrates inactivated TBE virus 20-40-fold with simultaneous purification from protein by 93%-95%. Inactivated virus was shown to keep on glass better than infectious virus. Gel filtration chromatography removes 99.2%-99.8% of protein impurities from inactivated TBE virus preparations giving a complete or nearly complete "yield" of virus particles. The sequential use of adsorption chromatography and gel filtration produced concentrated, most highly purified TBE virus preparations containing no more than 2 microgram/ml protein. Chromatographic virus preparations were immunologically active in experiments in laboratory animals.

Adsorption↗
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[Preparatory extraction and study of the structural proteins of rabies virus].

A technology for preparation of purified concentrates of rabies virus has been developed permitting to use simultaneously dozens of liters of tissue culture virus-containing fluid for the preparation of a concentrate. When concentrated 10(4)-fold or more, the degree of virus purification relative soluble proteins was at least 8 X 10(6). The virus yield in purified preparations was approximately 500 microgram of protein per 10 1 of the original culture fluid. Purification and preparation of a surface antigen of rabies virus, protein G, was also carried out. The yield of this protein in the resulting preparations was about 170 microgram per 10 1 of the culture fluid, the degree of purity being about 90%. A re-evaluation of the set, characteristics, and localization of rabies virus structural proteins was done. According to the experimental results, virus nucleocapsids contain 3 classes of proteins: L (a minor components with molecular weight of approximately 200,000), N (the dominating component, approximately 54,000) and NS (an intermediate component, approximately 47,000). The inner layer of virus lipoprotein membrane consists of protein M molecules (approximately 21,000) and apparently of protein A molecules (approximately 43,000) which are likely to be of cellular actin. The external spikes (peplomers) of virus particles contain molecules of glycoprotein G (approximately 65,000). Thus, the experimental results permit a conclusion that previously assumed significant differences in the protein composition of rabies viruses on the one hand and the other rhabdoviruses on the other are non-existent.

Centrifugation, Density Gradient↗
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[Concentrated purified vaccine against tick-borne encephalitis prepared by means of zonal ultracentrifugation. Development of the preparation].

A concentrated and purified (lyophilized) preparation with a high immunological activity was obtained from formalin-inactivated tissue culture suspensions of tick-borne encephalitis (TBE) virus. Its high immunogenicity was demonstrated in mouse protection tests against fatal challenge with TBE and in the studies of antibody production in monkeys of two species. The preparation was shown to contain no infectious TBE virus, to be safe for small laboratory animals and monkeys. Trials of the preparation in 19 volunteers demonstrated it to be well tolerated and to induce virus-neutralizing antibody production in man. It also sensitized lymphoid cells when inoculated subcutaneously with Al2O3. These experimental materials indicate the possibility of developing a highly potent vaccine against tick-borne encephalitis by concentration and purification of TBE particle suspensions.

Adult↗
PubMedDate not supplied

[The use of rocket immunoelectrophoresis for determining glycoprotein in concentrated rabies vaccines].

Rocket immunoelectrophoresis (RIE) was shown to be useful for the evaluation of glycoprotein (GP) content in concentrated rabies vaccines, and disintegron B., a zwitterionic detergent made in this country, for treatment of the vaccines for these evaluations. The values of GP content obtained by RIE and single radial immunodiffusion test were similar. The highest values were obtained for all the vaccines tested with anti-PM GP serum compared with anti-ERA and Vnukovo-32 GP serum. When anti-PM and anti-Vnukovo-32 GP sera were used for RIE two components were revealed in the vaccines. All the preparations under study contained soluble GP.

Betaine↗
PubMedDate not supplied

[Comparative analysis of tests on in vitro and in vivo quantitative assessment of the immunogenicity of tick-borne encephalitis vaccine].

Many-year routine use of EIA as an in vitro test demonstrated it as a highly reproducible and technological test for assessing the efficacy of vaccine against tick-borne encephalitis and its semiproducts at the intermediate stages of vaccine production. The reproducibility of mouse protection test is notably inferior to that of EIA.

Animals↗