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Biomedical subjects

L Ashok

Publications and source records attributed to L Ashok.

3 recordsLinked to original sources
PubMed1986

In vitro effects of cadmium chloride on preimplantation rat embryos.

Preimplantation rat embryos (8-cell, morulae, blastocysts) were incubated for 24 hr in vitro in a Brinster medium with or without cadmium chloride (1 microgram/ml). CdCl2 arrested the development of both the 8-cell and morulae into blastocysts. Morphologic observations revealed the 8-cell embryo to have shrunken cells and pyknotic nuclei. Morulae appeared smaller in size with fewer cells than comparable controls. CdCl2 was clearly cytotoxic to the early blastocysts, inner mass cells were most damaged with cell death, pyknosis, and accumulation of debris.

Animals↗
PubMed1985

Uptake and disposition of mirex in hepatocytes and subcellular fractions in CD1 mouse liver.

In vivo uptake and disposition of [14C]mirex by CD1 mouse liver subcellular fractions and cells of different nuclear ploidy were examined following single or multiple doses of mirex injected intraperitoneally. Significant amounts of mirex were rapidly taken up by liver (21-29%), suggesting that liver is one of the primary sites of accumulation of the chemical. Among subcellular fractions, mirex was predominantly distributed in mitochondria and microsomes in the irreversibly bound form (about 20%), although its levels fluctuated considerably with time. Mirex was completely dissociated with trichloroacetic acid treatment from both nuclear and plasma membrane fractions, although the total uptake by these fractions was markedly high. The time course of uptake and concentration-dependent disposition of mirex revealed that polyploid hepatocytes selectively accumulated higher amounts of the chemical (two to three times) compared to diploid hepatocytes. The increased affinity of polyploid cells to mirex may indicate a greater susceptibility of this cell type to the chemical insult and also may suggest a possible early involvement of polyploids in the tumorigenic process in rodent livers.

Animals↗