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Biomedical subjects

L Aguilera

Publications and source records attributed to L Aguilera.

At least 55 records · Page 3Linked to original sources

Interaction between thiopentone and sodium valproate. An in vitro and in vivo study.

The effect of sodium valproate on the binding of thiopentone to serum protein was studied in vitro. In the absence of valproate the unbound fraction of thiopentone was 15.2 +/- 0.64%, but with concentrations of valproic acid in excess of the therapeutic range, it increased to 22.42 +/- 1.65%. Scatchard plots for thiopentone in the absence and presence of valproate were investigated also. The barbiturate was bound by one group of binding sites with the association constant, Ka = 2.81 X 10(3) litre mol-1 and the number of binding sites (n) = 2.3. There was a marked decrease in this association constant in the presence of valproic acid (Ka = 1.82 X 10(3) litre mol-1), but without significant change in the number of binding sites (n = 2.13). Additionally, the effect of administration of sodium valproate i.v. on thiopentone anaesthesia was examined in rabbits. The recovery time from thiopentone was markedly longer in the presence of the anticonvulsant drug (17.0 min v. 37.1 min).

Anesthesia Recovery Period↗

Effects of three organophosphorus insecticides in the reproductive potential of Culex quinquefasciatus.

A Culex quinquefasciatus Say 1823 strain with resistant genes to organophosphates was tested in the laboratory to know the reproductive potential after exposure, as larvae, at the LC30 and LC70 (mg/l) of three organophosphorus insecticides: malathion, chlorpyrifos and methyl-pirimiphos. Data showed that fecundity was decreased significantly by malathion at LC30 = 0.0025 and LC70 = 0.0075, whereas fertility has a no significant decrement by chlorpyrifos and methyl-pirimiphos at the LC70 (0.000016, 0.00043). The sexual index was affected by chlorpyrifos and methyl-pirimiphos showing a greater number of adult females.

Animals↗

[Ecologic indexes in the surveillance system of Aedes aegypti (Diptera: Culicidae) in Cuba].

The ecological indexes called diversity (H') and equitability (J') of the mosquito species were determined in six of the most common reservoirs of the urban ecosystem in the Plaza de la Revolución municipality, City of Havana. Data analysis showed that according to the values of these indexes, the most dangerous reservoirs were tyres, larvitraps and artificial reservoirs in general. Reference is also made on the use of larvitraps in the surveillance system of Aedes aegypti and the detection and stabilization of mosquitoes such as Aedes mediovittatus and Culex migripalpus in the urban ecosystem. The variety of species ranged from 2 to 7 in the studied reservoirs which showed differences in the patterns of use of the resources in the urban ecosystem.

Aedes↗

[Proportional abundance of culicidae in the urban ecosystem of Havana City].

Proportional abundance of the four most common mosquito species was determined in six types of breeding places located in Plaza de la Revolución municipality, City of Havana from 1993-1996. Culex quinquifasciatus exhibited values over 0.60 except in tyres and larval traps in 1993 and 1994 whereas Aedes mediovittatus showed similar values in low tanks and artificial reservoirs in 1994 (0.43 and 0.56 respectively). It was also observed that this species prefers larval traps. Aedes aegypti and Culex migripaplpus had the lowest abundance values (0.01). These results allowed us to state that there is a sort of shared use by these species of those reservoirs where they breed and that Culex quinquefasciatus is the predominant one.

Animals↗

[Entomological surveillance over Aedes (S) aegypti and other culicids in Ciudad de La Habana, Cuba 1991-1996].

The results of the entomologic surveillance carried out from 1991 to 1996 in Boyeros municipality, City of Havana within the Program for Eradication of Aedes aegypti were analyzed. Data on mosquitoes fauna collected in the municipality by various sampling methods, larval survey, human bait, capture at rest and larval traps. Culex quinquefasciatus, Aedes mediovittatus and Aedes aegypti species were the most found by the different methods. It was proved that water tanks, man-made deposits, other kinds of tanks and low tanks were the most exploited resources by mosquitoes for their breeding in this municipality whereas larval surveys were the most sensitive method for detecting species since it contributed a greater variety of species. Reference is made to the search for a mechanism that help estimate adult populations from larval indices provided by surveys and the method of capture at rest is stressed as the most sensitive method for adult mosquitoes, particularly for dengue vector within the surveillance system aimed at this species.

Aedes↗

[The ecological succession of mosquito species in the town of Boyeros, Ciudad de la Habana 1994-1996].

An analysis was made on the incidence of Culicidae in Boyeros municipality, City of Havana from 1994 to 1996 based on the requirements of the national program for eradication of Aedes aegypti. A total of 13 species was detected in which Aedes mediovittatus predominated during 3 years of study, followed by Culex quinquefasciatus whereas for the first time Aedes albopictus species was found in the territory. People's Councils Wajay, Santiago de las Vegas and Armada exhibited the highest values of infestation by Culicidae in the municipality, therefore, they required that anti-Aedes program, operators paid more attention to this situation. It was found that depots preferred by Culicidae for breeding were larval traps followed by low tanks in 1994 and 1995 and by other reservoirs in 1996. A mediovittatus preferred larval traps whereas Cx. quinquefasciatus preferred low tanks except in 1996 when this species was mostly found in other tanks. 93% of all test-positive reservoirs found were colonized by only one species, whereas 7% presented larvae from 2 species or more. A. mediovittatus and C. quinquefasciatus were the most related, above all in low tanks.

Aedes↗

[Typing habitats of Aedes albopictus in Cuba and their association with other species of Culicidae, 1995-1998].

A typing of the habitats of Aedes albopictus in Cuba was made after its detection in 1995. It was observed in suburban areas of the Boyeros Municipality, in Havana City, where a study was conducted from October, 1995, to July, 1998. Most of the positive repositories were found outside the houses. Tins, tires, trees and plants were the places preferred for its breeding. It was found in the breeding places associated with Aedes mediovittatus and Culex quinquefasciatus, dominant species in these places. It was also observed a trend towards an increase of the number of repositories colonized only by the species under study. Reference was made to the phenomenon of competence between this species and the indigenous species present in such places.

Aedes↗

[Incidence of Aedes (S) aegypti and other Culicidae in the municipality of Playa, La Habana City].

An analysis was made on the incidence of Culicidae present in Playa Municipality, Havana City, during the intensive stage of the anti-aegypti campaign from June to July, 1997, according to its requirements. Culex quinquefasciatus was the predominant species with a total of 269 focuses. It was collected in all the People's Councils and in every type of repository, excepting clay repositories. The second most abundant species was the Aedes aegypti with 199 focuses, followed by Aedes mediovittatus with 67. The Almendares-Sierra, Ampliación-Almendares and Miramar People's Councils showed the highest values of Culicidae infestation in the municipality. The Aedes aegypti prevailed in the first and Cx. quiquefasciatus in the second. The artificial repositories, low tanks and other repositories had the highest number of mosquito focuses in the municipality. The Cx quinquefasciatus preferred the artificial depositories, whereas the A. aegypti preferred the low tanks. Most of the positive depositories were colonized by only one species (92.7% of the total). 7.3% corresponded to mixed focuses. It was found that Cx. quinqufasciatus and A. aegypti were the most associated species.

Aedes↗

[Biologic effect of diflubenzuron on Blattella germanica (Dictyoptera: Blattellidae)].

The Amejeiras strain of Blatella germanica (Dictyoptera: Blattellidae) kept in lab conditions since 1994 and susceptible to arpocarb, malathion and cipermethrin insecticides, was studied. This strain was treated with 4 diflubenzuron (Dimilin OMS 1804) concentrations supplied by feeding nymphs at their first stage of development, with the purpose of determining their effect on the survival, phenotype and period of nynmphal development of this species. The dosage-mortality line of regression was determined and the values of CE50 = 0.042% and CE95 = 0.357% were obtained. Sublethal effects were observed in the nymphs treated with the 4 tested concentrations whereas the morphogenetic effects in adult insects only occurred when they were exposed to higher concentrations (0.25% and 0.4%). The nymph development stage showed a highly significant increase when the diflubenzuron concentration was raised. (F = 11.64; p < 0.001).

Animals↗

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Anesthesia Department, Hospital↗