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Biomedical subjects

L Adler

Publications and source records attributed to L Adler.

At least 127 records · Page 7Linked to original sources
PubMed1985

Glycerol metabolism and osmoregulation in the salt-tolerant yeast Debaryomyces hansenii.

A glycerol-nonutilizing mutant of the salt-tolerant yeast Debaryomyces hansenii was isolated. When subjected to salt stress the mutant produced glycerol, and the internal level of glycerol increased linearly in proportion to increases of external salinity as in the wild-type strain. However, at increased salinity the mutant showed a more pronounced decrease of growth rate and growth yield and lost more glycerol to the surrounding medium than did the wild type. Uptake experiments showed glycerol to be accumulated against a strong concentration gradient, and both strains displayed similar kinetic parameters for the uptake of glycerol. An examination of enzyme activities of the glycerol metabolism revealed that the apparent Km of the sn-glycerol 3-phosphate dehydrogenase (EC 1.1.99.5) was increased 330-fold for sn-glycerol 3-phosphate in the mutant. Based on the findings, a scheme for the pathways of glycerol metabolism is suggested.

Glycerol↗
PubMed1985

Efficacy of propranolol in neuroleptic-induced akathesia.

The effects of propranolol, 20 to 30 mg/day, on neuroleptic-induced akathesia were compared with those of lorazepam, 2 mg/day, and periods of no treatment. Raters were blind to treatment condition. As reported in prior open studies, propranolol was found to be dramatically effective in reducing akathesia induced by neuroleptic treatment.

Adult↗
PubMed1985

Metastatic chemodectoma.

The authors report a case of primary chemodectoma with metastasis to the lungs. To date only 11 such cases have been reported in the literature.

Aged↗
PubMed1984

Pulmonary sequestration.

Pulmonary sequestration is a disturbance in embryonic development that results in a cystic mass of nonfunctioning lung tissue. The authors present a case of this disorder and review the pertinent literature.

Adult↗
PubMed1984

Blunt trauma to the chest.

Pulmonary contusions following blunt trauma often are visible roentgenographically immediately after injury. The case presented illustrates the need for prompt x-ray studies of the chest following blunt injury.

Adult↗
PubMed1978

Properties of alkaline phosphatase of the halotolerant yeast Debaryomyces hansenii.

The molecular weight of a partially purified alkaline phosphatase (orthophosphoric-monoester phosphohydrolase, EC 3.1.3.1) from the halotolerant yeast Debaryomyces hansenii was estimated to 110,000 by gel filtration. The isoelectric point determined by electrofocusing was at approximately pH 4.4. The enzyme had a broad specificity against phosphomonoesters and also attacked some acid anhydrides. Arsenate, molybdate, and orthophosphate acted as competitive inhibitors. Various metal-binding agents inhibited enzyme activity. A zinc addition almost completely reversed the EDTA inhibition. Magnesium stimulated enzyme activity and was required for maintenance of activity at high concentrations of Na+. Increasing glycerol concentration increased the value of the Michaelis constant (Km) and decreased the maximum velocity (V). Solutions equimolar in KCl and NaCl stimulated enzyme activity by increasing V, whereas the Km was almost unaffected by salt concentration. Enzyme extracted from cells cultured at low salinity was indistinguishable from that of cells grown in the presence of 2.7 M NaCl with respect to several criteria.

Alkaline Phosphatase↗
PubMed1976

Motivational effects of hunger on time estimation and delay of gratification in obese and nonobese boys.

Time estimates and ability to delay gratification of obese and nonobese boys were compared by a 2x2x2 factorial experiment in which hunger and edibility of incentives were manipulated. Hunger did not affect estimates of the obese boys, while it significantly shortened estimates made by the nonobese boys when the hunger drive was made either specific or nonspecific by varying incentive conditions. Although estimates of the nonobese boys were significantly shorter in the edible than nonedible incentive condition, edibility of incentives did not affect the estiamtes of the obese, suggesting a general rather than food-specific arousal in the nonobese and a general lack of response to internal cues in the obese. Immediate rewards were chosen significantly more often by obese than nonobese Ss. However, delay gratification in both obese and nonobese was unaffected by the hunger and edibility of incentive manipulations. This was attributed to uncontrolled cognitive variables.

Adolescent↗
PubMed1976

Kinetics of methoxyflurane biotransformation with reference to substrate inhibition.

The kinetics of biotransformation of methoxyflurane by rat hepatic microsomes in vitro was studied. The rate of biotransformation as measured by analysis of metabolites continued to increase even at near-saturation concentrations of the anesthetic. Methoxyflurane biotransformation followed either an unbounded curve with empirical formula y = a ln(bx + 1) or an asymptotic curve with formula (see article) No substrate inhibition was observed. Total fluoride Vmax of 135.1 mmumol F-/mg protein/30' was increased to 931.9 by phenobarbital induction; free fluoride Vmax from 39.2 to 403.2. Thus, enzyme induction shifted biotransformation to the production of greater amounts of inorganic free fluoride metabolites than organic fluoride-containing metabolites. Phenobarbital induction caused qualitative as well as quantitative alteration in the biotransformation of methoxyflurane.

Anesthesia, Inhalation↗
PubMed1976

Purification of alkaline phosphatase of the halotolerant yeast Debaryomyces hansenii.

Alkaline phosphatase (orthophosphoric monoester phosphohydrolase, EC 3.1.3.1) of the halotolerant yeast Debaryomyces hansenii was purified by a procedure involving cell disruption, DNAase treatment, ethanol precipitation, gel filtration, chromatography on DEAE-Sephadex, and preparative polyacrylamide gel electrophoresis. The specific activity was increased 1250-fold as compared to the activity of cell free extract. The total recovery was 30%. Various modifications of the growth conditions had slight or no effect on the yield of enzyme.

Alkaline Phosphatase↗