Biomedical subjects

L A Bradshaw

Publications and source records attributed to L A Bradshaw.

17 recordsLinked to original sources

PubMed2006

Biomagnetic characterization of spatiotemporal parameters of the gastric slow wave.

Certain gastric disorders affect spatiotemporal parameters of the gastric slow wave. Whereas the electrogastrogram (EGG) evaluates electric potentials to determine primarily temporal parameters, fundamental physical limitations imposed by the volume conduction properties of the abdomen suggest the evaluation of gastric magnetic fields. We used a multichannel superconducting quantum interference device magnetometer to study the magnetogastrogram (MGG) in 20 normal human subjects before and after a test meal. We computed the frequency and amplitude parameters of the gastric slow wave from MGG. We identified normal gastric slow wave activity with a frequency of 2.6 +/- 0.5 cycles per minute (cpm) preprandial and 2.8 +/- 0.3 cpm postprandial. In addition to frequency and amplitude, the use of surface current density mapping applied to the multichannel MGG allowed us to visualize the propagating slow wave and compute its propagation velocity (6.6 +/- 1.0 mm s(-1) preprandial and 7.4 +/- 0.4 mm s(-1) postprandial). Whereas MGG and EGG signals exhibited strong correlation, there was very little correlation between the MGG and manometry. The MGG not only records frequency dynamics of the gastric slow wave, but also characterizes gastric propagation. The MGG primarily reflects the underlying gastric electrical activity, but not its mechanical activity.

Electrodiagnosis↗

PubMed2005

Vector projection of biomagnetic fields.

Biomagnetic measurements are increasingly popular as functional imaging techniques for the non-invasive assessment of electrically active tissue. Although most currently available magnetometers utilise only one component of the vector magnetic field, some studies have suggested the possibility of obtaining additional information from recordings of the full magnetic field vector. Three projection techniques were applied to different biomagnetic signals for analysis of the three orthogonal components of the vector magnetic field. Vector magnetic fields obtained from fetal cardiac activity were projected into evenly spaced directions around a unit sphere. The vector magnetic field recorded from multiple intestinal current sources with independent temporal frequencies was then projected. Finally, an external reference signal from an invasive electrode was used to project the recorded vector magnetic fields due to gastric electrical activity. In each case, it was found that the information obtained by examination of the projected magnetic field vectors gave superior clinical insight to that obtained by analysis of any single magnetic field component.

Animals↗

PubMed2004

An anatomical model of the gastric system for producing bioelectric and biomagnetic fields.

Between 60 and 70 million people in the United States are affected by gastrointestinal disorders. Many of these conditions are difficult to assess without surgical intervention and accurate noninvasive techniques to aid in clinical assessment are needed. Through the use of a superconducting quantum interference device (SQUID) gradiometer, the weak magnetic field generated as a result of muscular activity in the digestive system can be measured. However, the interpretation of these magnetic recordings remains a significant challenge. We have created an anatomically realistic biophysically based mathematical model of the human digestive system and using this model normal gastric electrical control activity (ECA) has been simulated. The external magnetic fields associated with this gastric ECA have also been computed and are shown to be in qualitative agreement with recordings taken from normal individuals. The model framework thus provides a rational basis from which to begin interpreting magnetic recordings from normal and diseased individuals.

Digestive System Physiological Phenomena↗

PubMed2003

Biomagnetic detection of gastric electrical activity in normal and vagotomized rabbits.

We recorded the vector magnetogastrogram (MGG) due to gastric electrical activity (GEA) in normal rabbits using a Superconducting QUantum Interference Device (SQUID) magnetometer and measured the degree of correlation of the MGG with 24 channels of serosal electrodes. The vector magnetometer allows us to non-invasively record three orthogonal magnetic field components and project the recorded magnetic field vector into arbitrary directions. We optimized the magnetic field vector direction to obtain the highest possible correlation with each serosal electrode recording. We performed a vagotomy and examined spatial and temporal changes in the serosal potential and in the transabdominal magnetic field. We obtained spatial information by mapping the recorded signals to the electrode positions in the gastric musculature. Temporal evidence of uncoupling was observed in spectral analyses of both serosal electrode and SQUID magnetometer recordings. We conclude that non-invasive recordings of the vector magnetogastrogram reflect underlying serosal potentials as well as pathophysiological changes following vagotomy.

Animals↗

PubMed2001

Volume conductor effects on the spatial resolution of magnetic fields and electric potentials from gastrointestinal electrical activity.

An analysis of the relative capabilities of methods for magnetic and electric detection of gastrointestinal electrical activity is presented. The model employed is the first volume conductor model for magnetic fields from GEA to appear in the literature. A mathematical model is introduced for the electric potential and magnetic field from intestinal electrical activity in terms of the spatial filters that relate the bioelectric sources with the external magnetic fields and potentials. The forward spatial filters are low-pass functions of spatial frequency, so more superficial external fields and potentials contain less spatial information than fields and potentials near the source. Inverse spatial filters, which are reciprocals of the forward filters, are high-pass functions and must be regularised by windowing. Because of the conductivity discontinuities introduced by low-conductivity fat layers in the abdomen, the electric potentials recorded outside these layers required more regularisation than the magnetic fields, and thus, the spatial resolution of the magnetic fields from intestinal electrical activity is higher than the spatial resolution of the external potentials. In this study, two smooth muscle sources separated by 5cm were adequately resolved magnetically, but not resolved electrically. Thus, sources are more accurately localized and imaged using magnetic measurements than using measurements of electric potential.

Digestive System↗

PubMed2001

Spatial filter approach for comparison of the forward and inverse problems of electroencephalography and magnetoencephalography.

We present an analysis of the relative information content of cortical current source reconstructions from electroencephalogram (EEG) and magnetoencephalogram (MEG) forward calculations by examining the spatial filters that relate the internal sources with the externally measured electric potentials and magnetic fields. The forward spatial filters are seen to be low-pass functions of spatial frequency and spatial resolution degrades in external measurements. Inverse spatial filters may be used to reconstruct cortical sources from external data, but since they are high-pass functions of spatial frequency, they must be regularized to avoid instabilities caused by noise at higher spatial frequencies. The regularization process limits the spatial resolution of source reconstructions. EEG forward spatial filters fall off at lower spatial frequencies than MEG filters; hence, there is less information available in higher spatial frequencies resulting in lower spatial resolution in inverse reconstructions. The tangential component of the magnetic field provides even higher spatial resolution than can be obtained using the radial component. An accompanying article examines the surface Laplacian for both the EEG and the MEG.

Electric Conductivity↗

PubMed2001

Spatial filter approach for evaluation of the surface Laplacian of the electroencephalogram and magnetoencephalogram.

The surface Laplacian is a technique that has been utilized to improve the spatial resolution of the electroencephalogram (EEG) and the magnetoencephalogram (MEG). We investigate the amount of improvement to the spatial resolution afforded by the surface Laplacian by examining the spatial filters that describe the relationship between cortical current sources and the surface Laplacian. The surface Laplacian spatial filters extend into higher spatial frequencies than do raw signal spatial filters, particularly for EEG Laplacian spatial filters, indicating that substantial improvement in spatial resolution is possible. However, the response of the surface Laplacian operation to the nature and amount of noise in the raw EEG and MEG signals is of paramount importance. Spatially correlated noise, coupled with uncorrelated noise, requires additional regularization of inverse spatial filters resulting in a decrease in spatial resolution. Substantial improvements in spatial resolution may be obtained using the surface Laplacian techniques as long as correlated noise levels are small and raw signals have relatively high signal-to-noise ratios.

Computer Simulation↗

PubMed1999

Noninvasive detection of ischemic bowel.

PURPOSE: Acute mesenteric arterial occlusion is an abdominal catastrophe that carries high morbidity and mortality rates. Current diagnostic methods, however, lack sensitivity and specificity and do not provide information about the viability of the affected bowel. Early diagnosis and intervention would improve patient outcomes and survival rates. The basic electrical rhythm (BER) is the omnipresent electrical slow wave of the gastrointestinal tract that characterizes the underlying electrical activity of the bowel. BER frequency is known to fall with ischemia. Superconducting quantum interference devices (SQUIDs) can detect BER by measuring the magnetic fields generated by the electrical activity of the smooth muscle of the small bowel. The purpose of this study was to determine the ability of a SQUID to detect mesenteric ischemia in a free-lying section of small bowel in an animal model of acute superior mesenteric artery occlusion. METHODS: Seven adult male rabbits (six experimental and one control) were studied with transabdominal SQUID and electrode recordings during baseline and after the induction of mesenteric ischemia with balloon occlusion of the superior mesenteric artery. Continuous recordings were taken for 120 minutes of ischemia and analyzed with autoregressive spectral analysis to determine the BER frequency during specific time points of the study. Two independent investigators blinded to the experimental preparation examined the results to determine whether there was decreased BER frequency and thus ischemia. The results are expressed as mean +/- SEM, and paired t tests were used to determine statistical significance. RESULTS: BER was detected in all seven animals and fell from 10.7 +/- 0.5 cpm to 7.0 +/- 1.8 cpm after 30 minutes of ischemia in the magnetic channels (P <.05, with t test). The fall in BER was detected by the SQUID in all six experimental animals. The blinded observers correctly identified healthy and ischemic magnetic data recording, with a sensitivity of 94% and specificity of 100%. CONCLUSION: SQUIDs can noninvasively detect bowel ischemia early in a free-lying segment of small bowel in this animal model with a high degree of sensitivity and specificity.

Animals↗

PubMed1999

The human vector magnetogastrogram and magnetoenterogram.

Electrical activity in the gastrointestinal system produces magnetic fields that may be measured with superconducting quantum interference device magnetometers. Although typical magnetometers have detection coils that measure a single component of the magnetic field, gastric and intestinal magnetic fields are vector quantities. We recorded gastric and intestinal magnetic fields from nine abdominal sections in nine normal human volunteers using a vector magnetometer that measures all three Cartesian components of the magnetic field vector. A vector projection technique was utilized to separate the magnetic field vectors corresponding to gastric and intestinal activity. The gastric magnetic field vector was oriented in a cephalad direction, consistent with previously observed data, and displayed oscillatory characteristics of gastric electrical activity (f = 3.03 +/- 0.18 cycles/min). Although the small bowel magnetic field vector showed no consistent orientation, the characteristic frequency gradient of the small bowel electrical activity was observed. Gastric and intestinal magnetic field vectors were oriented in different directions and were thus distinguished by the vector projection technique. The observed difference in direction of gastric and intestinal magnetic field vectors indicates that vector recordings dramatically increase the ability to separate physiological signal components from nonphysiological components and to distinguish between different physiological components.

Analog-Digital Conversion↗

PubMed1999

Intestinal tachyarrhythmias during small bowel ischemia.

The electrical control activity (ECA) of the bowel is the omnipresent slow electrical wave of the intestinal tract. Characterization of small bowel electrical activity during ischemia may be used as a measure of intestinal viability. With the use of an animal model of mesenteric ischemia, serosal electrodes and a digital recording apparatus utilizing autoregressive spectral analysis were used to monitor the ECA of 20 New Zealand White rabbits during various lengths of ischemia. ECA frequency fell from 18.2 +/- 0.5 cycles per minute (cpm) at baseline to 12.2 +/- 0.9 cpm (P < 0.05) after 30 min of ischemia and was undetectable by 90 min of ischemia in all animals. Tachyarrhythmias of the ECA were recorded in 55% of the animals as early as 25 min after ischemia was induced and lasted from 1 to 48 min. Frequencies ranged from 25 to 50 cpm. These tachyarrhythmias were seen only during ischemia, suggesting that they are pathognomonic for intestinal ischemia. The use of the detection of ECA changes during intestinal ischemia may allow earlier diagnosis of mesenteric ischemia.

Animals↗

PubMed1998

Effects of mesenteric ischemia and reperfusion on small bowel electrical activity.

UNLABELLED: Previous studies involving basic electrical rhythm (BER) have not been carried out far enough to fully characterize the relationship between mesenteric ischemia and BER. The phenomenon of reperfusion injury has also not been correlated with BER activity. The goal of this study was to characterize changes in BER during mesenteric ischemia and reperfusion and to correlate them with changes in pathology. METHODS: Serosal electrodes were used to record the electrical activity of rabbit jejunum (n = 20) at baseline, during ischemia (90-210 min), and during reperfusion (120-240 min). BER frequency and amplitude were monitored, and biopsies were taken at the end of ischemia and reperfusion. A pathologist blinded to the specimen identity graded the histology on a scale of 0 (no changes) to 6 (transmural necrosis). Paired t test, the Kruskal-Wallis test of non-parametric ranks, and Fisher's r to z test were used for statistical significance where appropriate. RESULTS: BER frequency and amplitude fell significantly after 15 min of ischemia and became undetectable by 90 min of ischemia in all animals. The likelihood that BER would return during reperfusion was highly correlated with length of ischemia (r = 0.99). Longer periods of reperfusion were associated with increasing pathologic grade. CONCLUSIONS: BER frequency and amplitude are very sensitive to ischemia and their changes occur well before histopathologic changes. The variation in electrical activity of the small bowel during ischemia and reperfusion is a dynamic process that reflects the metabolic state of the smooth muscle. If electrical activity of the bowel is to be used for assessment of viability, continuous recordings more accurately reflect the metabolic state of the smooth muscle.

Animals↗

PubMed1997

Superconducting quantum interference device magnetometer for diagnosis of ischemia caused by mesenteric venous thrombosis.

Although mesenteric venous thrombosis carries a better prognosis than arterial thrombosis, mortality and morbidity are still high. Previous studies have shown that the basic electrical rhythm (BER) of the bowel decreases early after induction of arterial ischemia. Furthermore, our studies have shown that these changes occur prior to pathologic changes and that they can be recorded noninvasively using a superconducting quantum interference device (SQUID). SQUIDs measure magnetic fields that are created by the electrical activity of the gastrointestinal smooth muscle and have been used to measure the BER of the small intestine in human volunteers. This study was conducted to determine if a SQUID could be used for early noninvasive detection of mesenteric venous ischemia in an animal model. Simultaneous recordings from serosal electrodes and a SQUID outside the abdomen were taken from anesthetized New Zealand rabbits. Recordings were made for 15 minutes before and 90 minutes after injection of thrombin into the superior mesenteric vein. The basic electrical rhythm of the small bowel dropped from 16.42 +/- 0.69 to 8.80 +/- 0.74 cycles per minute at 30 minutes and to 6.82 +/- 0.722 after 90 minutes (p < 0.0001, paired t-test). The correlation coefficient between the SQUID and electrical recordings was 0.954 (p < 0.0001). These data suggest that the ischemia caused by mesenteric venous thrombosis results in changes in the bioelectrical activity, which can be noninvasively detected using a SQUID.

Animals↗

PubMed1997

Correlation and comparison of magnetic and electric detection of small intestinal electrical activity.

The small intestinal basic electrical rhythm (BER) was detected simultaneously with serosal electrodes and a transabdominal superconducting quantum interference device (SQUID) magnetometer in anesthetized rabbits. We induced mesenteric ischemia to correlate serosal electrode recording of changes in BER with the SQUID magnetometer. The BER frequency was obtained by spectral analysis of the data using Fourier and autoregressive techniques. There was a high degree of correlation (r = 0.96) between the BER frequency determined using the serosal electrodes and the BER frequency ascertained from SQUID data. Additionally, the effects of an electrical insulator on the external electric and magnetic fields were studied in the rabbit model. The presence of an insulator profoundly attenuates external electric potentials recorded by cutaneous electrodes but does not significantly affect external magnetic fields or serosal potentials. We conclude that SQUID magnetometers could noninvasively record small intestinal BER that was highly correlated with the activity recorded by invasive serosal electrodes. The advantages of magnetic field measurements have encouraged us to investigate clinical applications.

Abdomen↗

PubMed1996

Magnetoenterography (MENG): noninvasive measurement of bioelectric activity in human small intestine.

The basic electrical rhythm (BER) of the gastrointestinal tract creates minute magnetic fields that have been measured in animals using a Superconducting QUantum Interference Device (SQUID) gradiometer. The aim of this study was to measure noninvasively the biomagnetic fields of human stomach and small intestine. Twenty-one human volunteers were studied using a 37-channel SQUID gradiometer positioned over the epigastrium and umbilicus. In one volunteer additional biomagnetic recordings were performed in order to map the spatial variation of the biomagnetic fields. Cyclical waveforms consistent with gastric BER [3.0+/-0.5 cycles per minute (cpm)] and small intestine BER (10.26+/-1.74 cpm) were seen in the epigastrium and umbilicus, respectively. The mapping study identified the expected frequency gradient (12.0 cpm in duodenum, 11.3 cpm in jejunum, to 9.7 cpm in ileum) within the small intestine. Noninvasive recordings of human gastric and small intestinal BER can be obtained using a SQUID gradiometer.

Adult↗

PubMed1995

Noninvasive diagnosis of mesenteric ischemia using a SQUID magnetometer.

OBJECTIVE: The authors assessed the ability of a Superconducting Quantum Interference Device (SQUID) magnetometer to noninvasively detect mesenteric ischemia in a rabbit model. SUMMARY BACKGROUND DATA: Superconducting Quantum Interference Device magnetometers have been used to detect magnetic fields created by the basic electrical rhythm (BER) and to detect changes in BER of exteriorized bowel of anesthetized rabbits during mesenteric ischemia. METHODS: The BER of rabbit ileum was noninvasively measured transabdominally using a SQUID magnetometer and compared with the electrical activity recorded with surgically implanted serosal electrodes before, during, and after snare occlusion of the superior mesenteric artery. RESULTS: Transabdominal SQUID recording of BER frequency was highly correlated to the measurements obtained with electrodes (R = 0.91). Basic electrical rhythm frequency decreased from 16.4 +/- 0.8 to 8.3 +/- 0.3 cpm (p < 0.001) after 25 minutes of ischemia. Reperfusion of ischemic bowel resulted in recovery of BER frequency to 14.3 +/- 0.4 cpm 10 minutes after blood flow was restored. CONCLUSIONS: A SQUID magnetometer is capable of noninvasively detecting mesenteric ischemia reliably and at an early stage by detecting a significant drop in BER frequency. These positive findings have encouraged the authors to continue development of clinically useful, noninvasive, detection of intestinal magnetic fields using SQUID magnetometers.

Animals↗

PubMedDate not supplied

Changes in intestinal electrical activity during ischaemia correlate to pathology.

The gastrointestinal tract possesses an omnipresent electrical slow wave called the basic electrical rhythm (BER). It has been shown that the frequency of the BER falls during intestinal ischaemia. The correlation between changes in the BER and pathology that occur during acute ischaemia are not completely understood. To study this, the electrical activity of the ileum in 14 adult male rabbits was recorded during ischaemia. At baseline, 60, 120 and 210 minutes of ischaemia, segments of bowel were resected for histopathologic evaluation. The BER frequency was determined using the Fast Fourier Transformation (FFT) spectral analysis. The BER amplitude and FFT spectral power were also determined. The results showed significant decrease (p<0.05, Student's T-test) in the BER frequency, amplitude, and spectral power at all time points. Between 60 and 120 minutes, while there was a decrease in BER activity the pathologic grade remained the same (focal loss of surface epithelium). By 210 minutes of ischaemia when the BER could not be recorded, there was diffuse mucosal infarction. The results indicate that changes in the electrical activity of the bowel during acute mesenteric ischaemia occurred prior to the pathologic changes. The presence of electrical activity indicates that there was viable bowel. Thus it should be possible to use recordings of electrical activity to evaluate bowel viability during acute ischaemia.

Animals↗

PubMedDate not supplied

Histopathologic changes during mesenteric ischaemia and reperfusion.

The basic electrical rhythm (BER) of the intestine is known to decrease during mesenteric ischaemia. Some studies have reported the relationship between the BER and the pathologic changes that occur in the bowel during vascular injury. However, these changes have not been completely elucidated. This study describes the histopathologic pattern when the rabbit small intestine was subjected to ischaemia of varying time lengths (30-150 minutes) and subsequent reperfusion for six hours. Intestinal biopsies were taken at baseline, at the end of ischaemia, and at hourly intervals during reperfusion. Microscopic examination of the biopsies revealed evidence of progressive infarction of the mucosa during ischaemia. There was an acute worsening of the pathology during reperfusion, the severity being greater when reperfusion was preceded by longer periods of ischaemia. These changes were statistically significant. The observed pattern in this study shows clearly that reperfusion injury is reflected in the histopathologic response and that this is worse in severity than the response to ischaemia. Studies of longer duration should further clarify the picture during recovery in ischaemia/reperfusion injuries of the bowel.

Animals↗