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Biomedical subjects

Koki Takahashi

Publications and source records attributed to Koki Takahashi.

2 recordsLinked to original sources

[Computer management to avoid errors in transfusion].

Even with the great progress in blood safety, such as the nucleic acid amplification test(NAT) for HBV, HCV and HIV, ABO-mismatched transfusion still occurs in the 21st century. Computer crossmatch between the patient's blood type already registered on computer, the patient's blood type as examined just before transfusion and the blood type labeled on blood bags all help avoid misunderstanding of blood type at shipping. Computerized management of the transfusion services database is also effective in promoting appropriate transfusion or autologous transfusion. Since mistakes can occur anywhere in the system, the Japanese Society of Blood Transfusion(JSBT) made a poster showing six steps where errors were most likely. They then distributed a questionnaire about mismatched transfusion to 777 major hospitals and made it clear that the main cause of mistakes was confirmation errors at bedside before transfusion. In response to the findings, JSBT created a manual for performing exactly matched and safe transfusions, including a manual for transfusion in emergencies, as well as first-line treatment procedures in case of major mismatched transfusion. In order to avoid mistakes at bedside, a barcode matching system between the patient's wristband and blood bag is ideal. It will also be useful for comprehensive risk management in modern medicine.

Blood Group Incompatibility↗

Enhanced activities and gene expression of phosphodiesterase types 3 and 4 in pressure-induced congestive heart failure.

Phosphodiesterase (PDE) was shown to be downregulated in the failing hearts of transplant recipients, while it was upregulated in hypertrophied hearts induced by isoproterenol and calsequesterin overexpression. We examined the time course of gene expression and the activity of PDE3 and PDE4 in an animal model of salt-induced hypertension, left ventricular hypertrophy, and congestive heart failure (CHF). Dahl salt-sensitive (DS, n = 25) and salt-resistant rats (DR, n = 25) were fed with an 8% NaCl diet after the age of 6 weeks. At 11 weeks (hypertension and hypertrophy stage in DS), PDE4 activity in the heart was higher in DS than in DR. At 18 weeks (hypertension and CHF stage in DS), both PDE3 and PDE4 activity in both the heart and aorta was approximately twofold higher in DS than in DR. The ratios of PDE3 and PDE4 mRNA to GAPDH mRNA in the heart were both approximately twofold higher in DS than in DR at 11 and 18 weeks. The cardiac cyclic adenosine monophosphate content and plasma nitric oxide concentration were higher in DS than in DR at 11 weeks but both of them were lower in DS than in DR at 18 weeks of age. In this animal model, gene expressions of PDE3 and PDE4 were augmented from the hypertrophic stage. PDE3 and PDE4 activities were subsequently enhanced in the CHF stage and seemed to contribute to the development and exacerbation of CHF.

3',5'-Cyclic-AMP Phosphodiesterases↗