Search PubMed⌕ Search

Biomedical subjects

K van Dam

Publications and source records attributed to K van Dam.

99 records · Page 6Linked to original sources
PubMed1968

Changes of total water and sucrose space accompanying induced ion uptake or phosphate swelling of rat liver mitochondria.

1. Total water exchangeable with tritiated water and sucrose space were measured in rat liver mitochondria during the uptake of K(+) induced by valinomycin and the release caused by nigericin. The K(+) content and the sucrose-inaccessible water rose and fell together. 2. Swelling resulting from phosphate addition in a medium of high K(+) concentration was associated mainly with increased sucrose-accessible water, which carried dissolved K(+). This change was reversed by addition of ATP. 3. The response of the sucrose-inaccessible space to changed osmolarity was qualitatively that expected if the mitochondrial K(+) is assumed to be present in this space with a univalent anion. 4. It is brought out that the light-scattering method fails to distinguish between changes in sucrose space and in sucrose-inaccessible space, which in the present experiments could be altered respectively by phosphate (in high K(+) solution) and by cation uptake induced by antibiotic.

Acetates↗
PubMedDate not supplied

Quantification of control of microbial metabolism by substrates and enzymes.

The control of substrates or enzymes on metabolic processes can be expressed in quantitative terms. Most of the experimental material found in the literature, however, has been obtained under non-standardized conditions, precluding definite conclusions concerning the magnitude of control. A number of representative examples is discussed and it is concluded that a quantitative analysis of the factors that control metabolism is essential for understanding the microbial behaviour.

Adenosine Triphosphate↗