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Biomedical subjects

K Yuasa

Publications and source records attributed to K Yuasa.

At least 37 records · Page 2Linked to original sources
PubMed1990

Presence of autoantibody against nasal mucosal cells in patients with Wegener's granulomatosis.

We examined whether or not patients with Wegener's granulomatosis have autoantibodies against the respiratory tract and kidney, by using the direct avidin-biotin-glucose oxidase complex method. On immunostaining, the IgG fractions obtained from the sera of 2 patients with Wegener's granulomatosis and of 7 controls were coupled with biotin, and each couple was used as the primary antibody. The serum IgG sample from 1 patient with Wegener's granulomatosis definitely reacted with epithelial cells of the nasal mucosa, with the strongest reaction in basal layer. The sample from another patients with Wegener's granulomatosis showed a similar but weaker reaction. Control sera showed negative reactions in 6 subjects (3 with rheumatoid arthritis, 1 with allergic granulomatous angiitis and 2 normal volunteers) and a very weak reaction in 1 patient with poly-arteritis nodosa. These observations suggest the presence of an autoantibody reacting with some cell components of the nasal epithelium in patients with Wegener's granulomatosis.

Adult↗
PubMed1990

Native valve endocarditis caused by Acinetobacter calcoaceticus subspecies lwoffi.

A case of native valve endocarditis due to Acinetobacter calcoaceticus in a patient with dental caries is presented. The aortic, mitral and tricuspid valves were affected and showed vegetation by echocardiography in the affected valves. In spite of a good response to antibiotic therapy, multiple embolisms resulted in the patient's death. An autopsy confirmed the vegetation, which was calcified and contained no bacteria.

Acinetobacter Infections↗
PubMed1990

Idiopathic myocarditis associated with T-cell subset changes and depressed natural killer activity.

A 65-year-old man with idiopathic myocarditis is described. He was admitted with symptoms of acute heart failure. Examination revealed left ventricular hypokinesis. Results of an endomyocardial biopsy showed "resolving myocarditis". Immunological studies of the peripheral blood showed a low helper-suppressor (CD4/CD8) ratio on admission and depressed natural killer (NK) cell activity coincident with the onset of myocarditis. We considered that this immunological imbalance may have occurred during the progression of myocarditis to dilated cardiomyopathy.

Aged↗
PubMed1990

Effects of thyroid hormone, TRH and TSH on pro-TRH concentrations in various organs of rats.

The effects of large doses of thyroid hormone, thyrotropin (TSH) or thyrotropin-releasing hormone (TRH) on pro-TRH concentrations in the rat hypothalamus, cerebrum, cerebellum, brain stem, stomach and eye were studied. The rats were administered T4 (2.5 mg/kg), T3 (375 micrograms/kg), TRH (1.0 mg/kg) or bovine TSH (1.25 IU/kg) i.p. and seven rats in each subgroup were decapitated at 4 or 24 h after the injection. Pro-TRH, TRH, TSH and thyroid hormone were measured by each radioimmunoassay. Immunoreactive pro-TRH (ir-pro-TRH) and immunoreactive TRH (ir-TRH) were found in the hypothalamus, cerebrum, brain stem, stomach and eye. Ir-pro-TRH concentrations in the hypothalamus decreased significantly after T4 and T3 injection and tended to decrease after TRH and TSH injection, but not significantly. In contrast, ir-pro-TRH concentrations in other tissues showed no changes after T4, T3, TRH or TSH injection. Ir-TRH concentrations in tissues did not change significantly after these hormone injection. The plasma TSH levels decreased significantly, while these of thyroid hormone increased significantly after thyroid hormone injection. Elution profile of acetic acid extracts of hypothalamus, stomach or eye was identical to that of synthetic pro-TRH. The findings suggest that the large dose of thyroid hormone inhibits pro-TRH synthesis in the hypothalamus, and that TRH synthesis in the tissues except the hypothalamus may not be regulated by thyroid hormone.

Animals↗
PubMed1990

[Two cases of pulmonary alveolar proteinosis with increased CEA].

Two cases of pulmonary alveolar proteinosis (PAP) with increased CEA were reported. Case 1 was a 62-year-old male with suspected pulmonary fibrosis, who was transferred to our hospital. Laboratory findings on admission revealed 31.3 ng/ml of CEA. Because he had severe dyspnea, lung biopsy was not carried out. His condition gradually deteriorated and he died of respiratory failure. Autopsy revealed he had PAP and no malignancy. Case 2 was a 48 year-old male referred to our hospital because of dyspnea. Serum CEA was 52.8 ng/ml. Microscopic examination of a transbronchial lung biopsy showed PAP. The level of CEA in bronchoalveolar lavage fluid was 151 ng/ml. Unilateral whole lung lavage was performed twice. With the improvement of chest X-ray findings, serum levels of CEA fell to normal level. The molecular weight of CEA in bronchoalveolar lavage fluid was 180,000. Immunochemical staining of CEA in lung revealed nonspecific findings.

Carcinoembryonic Antigen↗
PubMed1990

[An autopsy case of chronic pulmonary thromboembolism associated with congenital portal hypoplasty and pulmonary artery aneurysm].

A 44 year-old woman died after a history of chronic right heart failure for 25 years. Roentgenological studies showed marked pulmonary aneurysm, and hemodynamics at rest revealed severe pulmonary hypertension. The diagnosis of chronic pulmonary thromboembolism was made by MRI which identified a high intensity mass in the dilated right pulmonary artery. At autopsy, a large organized thrombus adhered to the atherosclerotic pulmonary artery, and pulmonary thromboembolism or hemorrhagic infarction were identified. Microscopic examination revealed intimal and medial proliferation in small arteries and plexiform lesions. In addition, hypoplasty of the portal vein and portacaval shunt, which were thought to be congenital, were present. It has been recognized that the portacaval shunt can be attributed to pulmonary hypertension. In this case it was considered that the main cause of pulmonary thrombus formation was both pulmonary atherosclerosis and pulmonary artery aneurysm caused by prolonged pulmonary hypertension. This is the first case of chronic pulmonary thromboembolism associated with congenital hypoplasty of portal vein.

Adult↗
PubMed1990

[Image analysis of morphologic features of the corneal endothelium including hexagonality].

We developed an analysis system for the evaluation of corneal endothelial photographs. Morphological parameters including hexagonality were analyzed semiautomatically with this system. The image analyzer used consists of an image processor MC68000 and image memories 512 x 512 x 17bytes in size. The algorithm that measures the number of apices of each cell is as follows. First, all crossing points of cell borders are detected. Then, each cell is enlarged in order by three pixels in eight directions. The number of crossing points which are overlapped with an enlarged cell is detected as the number of apices of the cell. The analysis time was 7.44 +/- 1.48 min. (mean +/- standard deviation) for 40 endothelial photographs which needed no manual trace for contrast enhancement.

Cell Count↗
PubMed1990

[Histochemical study of proliferating cells in the spleen in mice with acute hepatic injury].

When heat-killed Propionibacterium acnes (P. acnes) and lipopolysaccharide (LPS) were injected into mice, liver cell necrosis with infiltrating neutrophils and macrophages were induced. Proliferating cells in the spleen were histochemically investigated. P. acnes injection rapidly produced hyperplasia of neutrophils and macrophages in the red pulp of the spleen. The proportion of Bromodeoxyuridine positive cells reached a peak at 5 days after injection of P. acnes. These results indicate that proliferating cells in the spleen after injection of P. acnes are mainly neutrophils and macrophages, which are the same kinds of infiltrating cells into the liver after injection of P. acnes and LPS.

Animals↗
PubMed1989

A growth-promoting factor for human myeloid leukemia cells from horse serum identified as horse serum transferrin.

A growth-promoting factor for human myeloid cells was purified to apparent homogeneity from horse serum by a combination of gel filtration, blue Sepharose affinity chromatography, Mono Q anion-exchange chromatography, Mono P chromatofocusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis. The growth promoter was an iron-bound, single glycopolypeptide chain with a molecular weight of 84,000, an isoelectric point of 5.4 and an amino terminal sequence of Glu-Gln-Thr-Val-Arg-Trp-Cys-Thr-Val-Ser-Asn-His-Glu-Val-Ser-Lys-. According to the results of the amino acid sequence, iron binding ability and physicochemical properties, we identified the growth-promoting factor as horse serum transferrin. It was highly active in promoting the proliferation of a human monocytic leukemia cell line, THP-1, as well as of two other human myeloid cell lines, HL-60 and K-562. It had the same activity in proliferating THP-1 cells as 5% fetal calf serum-supplemented medium. Horse serum transferrin could be substituted for human or bovine serum transferrin.

Amino Acid Sequence↗
PubMed1989

Three cases of Wegener's granulomatosis treated with an antimicrobial agent.

Sulfamethoxazole-trimethoprim, an antimicrobial agent, was used to treat three cases of cytotoxic agent-resistant Wegener's granulomatosis; one case was refractory to a regimen consisting of azathioprine and prednisolone, and in another two cases the therapy was insufficient to control the disease. By the introduction of sulfamethoxazole-trimethoprim, which has an advantage of fewer side effects compared with the conventional agents, into the regimen, the disease condition was markedly improved in all cases within four months. Sulfamethoxazole-trimethoprim is thus considered as one of the most promising drugs to treat, especially, cytotoxic agent-resistant Wegener's granulomatosis. The possible underlying mechanism of the effect of sulfamethoxazole-trimethoprim is also described.

Adult↗
PubMed1989

Analysis of submandibular gland sialograms in Sjögren's syndrome.

We report here the sialographic appearances of the submandibular gland in both primary and secondary Sjögren's syndrome (SS). In order to compare these with those of the parotid gland, we defined new criteria for the staging for SS based on the degree of depiction of the peripheral ducts. In 24 out of 74 cases the submandibular sialogram showed more advanced, destructive disease compared with the parotid. This study suggests that although SS involves the parotid gland first, it progresses in the submandibular gland more rapidly.

Adult↗
PubMed1989

[Study on two-dimensional tooth mobility].

Tooth mobility was measured with the use of a two-dimensional displacement measuring system. This small-sized system can measure the displacement both in the direction of the tooth axis and the buccolingual direction simultaneously with no contact. As test teeth, lower right first premolars with no clinical mobility were examined. The load was applied from the lingual to the buccal direction, to the vertical direction as well as from the buccal to the lingual direction. The results obtained were as follows: 1. This system possesses sufficient accuracy and reproducibility for tooth mobility measurement (measurement range 500 microns, resolving power 1 microns). 2. Most of the load-displacement curves showed diphasic displacement. 3. As to the amount of displacement in the buccolingual direction, 3 of 8 cases showed greater values by the load in the buccal direction and the remaining 5 by the load in the lingual direction. 4. As to the displacement direction of loaded teeth, the lingual and buccal load displaced teeth to the lingual-coronal and buccal-radicular directions respectively. Teeth under the vertical load were displaced almost to the radicular and slightly to the buccal direction. 5. As to the direction of tooth displacement in relation to load directions, it appeared that the teeth not only rotated within the dental sockets but also indented or extruded into the dental sockets.

Dental Stress Analysis↗