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Biomedical subjects

K Yoshimura

Publications and source records attributed to K Yoshimura.

At least 469 records · Page 26Linked to original sources
PubMed1986

[Immunohistochemical studies of fetal and maternal endocrine pancreases during pregnancy and the puerperium in streptozotocin-induced diabetic rats].

In order to clarify the influence of the diabetic state on the structure of maternal and fetal endocrine pancreases, the distribution of alpha, beta and delta cells in the islets of Langerhans (IL) was investigated by PAP methods in normal and streptozotocin (STZ)-induced diabetic rats. The size of the IL significantly increased during pregnancy and on day 14 of puerperium in normal and diabetic maternal rats. The total cell numbers of IL also increased during pregnancy but decreased in puerperium in both groups. Although the number of beta cells was reduced in STZ-treated rats, they could increase during pregnancy as in the normal group. The number of beta cells kept increasing in puerperium in the diabetic group, but not in the normal group. The number of alpha and delta cells in diabetic rats was greater than in normal rats but did not change remarkably during pregnancy and in puerperium. The IL of fetuses from diabetic mothers were slightly greater in size and number than those from normal mothers. The number of fetal beta cells from normal mothers was somewhat greater than that from diabetic mothers. The number of fetal alpha and delta cells from diabetic mothers was slightly greater than that from normal mothers. These findings, therefore, suggest that diabetic IL could adapt themselves to pregnancy-induced metabolic changes.

Animals↗
PubMed1986

[Three-dimensional analysis of colonic adenoma and of carcinoma in adenoma with scanning electron microscope and sequential serial sectioning].

Three-dimensional structures were analyzed on 32 human colonic adenomas, 5 hyperplastic polyps, 4 normal colonic mucosa and 5 advanced colonic carcinomas with Scanning Electron Microscope (SEM) and with sequential serial sectioning. After SEM observation, the samples were softened with 2% NaHCO3 and observed with histological serial sectioning. By combining both techniques, that is combined analysis of outer structures for SEM and inner structures for serial sectioning, three dimensional analysis of the colonic adenoma and carcinoma in adenoma were successfully completed. The colonic adenomas were classified into 6 types based on the surface structure observed by SEM as Type I (circular type), Type II (long-elliptical type), Type III (branched type), Type IV (gyral type), Type V (wrinkle type) and Type VI (complicated type). Classified Types I, II, III and IV were observed in tubular adenoma, and grades of atypia seemed to be increased as increasing type numbers. Type V, in tubulo-villous adenoma and villous adenoma, were found out and showed severer atypia than observed in Type IV. Among 32 adenomas, 3 portions of focal cancers were detected, and the surface structures of them had highly complicated patterns as shown with Type VI.

Adenoma↗
PubMed1986

[Studies on somatomedin C in diabetic pregnancies].

Somatomedins have been shown to have potent mitogenic activities in cultured cells and also promote individual growth. We studied the concentrations of somatomedin C in diabetic pregnant women and rats. Serum and liver somatomedin C were measured by RIA double antibody method, after extraction with an ODS silica column. Serum somatomedin C concentrations were 20.45 +/- 5.14 nM/l (mean +/- S.D.) in nonpregnant normal women. In nonpregnant diabetic women, these were 16.96 +/- 4.37 nM/l, which were significantly lower than those of normal nonpregnant women. The concentrations of pregnant normal and diabetic women were similar to those of nonpregnant normal women. Maternal concentrations of somatomedin C significantly correlated with infant weight (r = 0.41, p less than 0.05, n = 41). Serum somatomedin C concentrations in the normal infants were 6.45 +/- 1.97 nM/l, which were considerably lower than those of their mothers. In the infants from diabetic mothers, these were 9.17 +/- 3.28 nM/l, which were significantly higher than those of normal infants. Rat serum somatomedin C concentrations which were 15.86 +/- 2.37 nM/l in nonpregnant normal rats, tended to increase during pregnancy. The mother's concentration significantly correlated with fetus weight (r = 0.491). Liver somatomedin C levels decreased in diabetic and pregnant states. Fetal rats had lower hepatic somatomedin C, which was about 10% of the mother's levels. These were higher in the fetuses from diabetic mothers. These finding suggested that the mother's somatomedins might contribute to fetal growth.

Animals↗
PubMed1985

Complete amino acid sequence of a unique protein related to the variable domain of lambda light chain from a case with Fanconi syndrome.

The complete amino acid sequence has been determined of a unique protein from a 55-years-old female with multiple myeloma associated with Fanconi syndrome. It existed in a monomer form with an apparent molecular weight of 10K daltons, and was consisted of 106 amino acid residues. The sequence was characteristic of the V-region of lambda light chains and was highly homologous with that of the first 106 residues of V lambda III subgroup. The presence of an intact light chain as well as a 13K daltons fragment, corresponding to the entire C-region, strongly suggests that the unique component is a catabolic product from the intact light chain rather than an aberrant product of synthesis.

Amino Acid Sequence↗
PubMed1985

Effects of prostaglandin E1 and isosorbide dinitrate on acute hypoxic pulmonary vasoconstriction in conscious sheep.

The pulmonary vascular effects of prostaglandin E1 (PGE1) and isosorbide dinitrate (ISD) on acute hypoxic pulmonary vasoconstriction in conscious sheep were studied. While the animals inhaled room air or hypoxic gas (O2:N2 = 1:9), PGE1 (0.5 microgram/kg/min) and ISD (10 microgram/kg/min) were administered intravenously for 20 min, using an infusion pump. The changes of pulmonary arterial pressure (PPA) or pulmonary vascular resistance (PVR) in room air due to PGE1 were not significant, but in the hypoxic condition, PGE1 decreased PPA by 14.7% (p less than 0.05) and PVR by 14.5% (p less than 0.05). The effect of PGE1 in room air on systemic arterial pressure (PSA) remained nearly constant, whereas slight decreases in systemic vascular resistance (SVR) were noticed. In hypoxic condition, PSA levels were slightly decreased by PGE1, but SVR indicated a slight degree of delayed rise. When ISD in room air was administered, the values of PPA, PVR, PSA and SVR decreased by 9% (p less than 0.05), 10.4% (p less than 0.05), 17.7% (p less than 0.05) and 21.8% (p less than 0.05), respectively. In hypoxic condition, ISD decreased the values of PPA, PVR, PSA and SVR by 14.7% (p less than 0.05), 15.8% (p less than 0.05), 10.2% (p less than 0.05) and 6.7% (p less than 0.05), respectively. In summary in hypoxic condition both PGE1 and ISD cause similar decreases of PPA and PVR. Furthermore, there was a very little decrease in PSA during PGE1 infusion in the hypoxic condition.(ABSTRACT TRUNCATED AT 250 WORDS)

Alprostadil↗
PubMed1985

Augmentation of isoproterenol-stimulated tissue cyclic AMP level by cholinergic agonists in rat parotid gland.

It was found that methacholine and carbamylcholine, in addition to their known inhibitory effect, augmented the effect of isoproterenol on tissue cyclic AMP accumulation. The effect of methacholine was dose dependent, and significant augmentation was obtained at 0.1 microM with the maximum being attained at about 0.5 microM, whereas more than 10 microM were required to obtain the inhibitory effect. Atropine completely blocked the effect of methacholine. Similar augmentation of isoproterenol effect was obtained by oxotremorine and pilocarpine. Oxotremorine, however, did not inhibit the effect of isoproterenol. Difference in the effect between methacholine or carbamylcholine and oxotremorine was observed in their binding property to cholinergic receptors. A23187 augmented the effect of isoproterenol in a dose-dependent manner. Oxotremorine and A23187 augmented the effect of isoproterenol in the presence of isobutylmethylxanthine, but they did not augment the effect of forskolin and isobutylmethylxanthine on tissue cyclic AMP accumulation. Cholinergic agonist- and A23187-induced augmentation was abolished by omission of calcium in the medium. These results suggest that the augmentation is due to activation of adenylate cyclase, which is mediated by an increase in concentration of intracellular calcium.

1-Methyl-3-isobutylxanthine↗