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Biomedical subjects

K Yonenobu

Publications and source records attributed to K Yonenobu.

At least 37 records · Page 2Linked to original sources

Pathology of ossification of the posterior longitudinal ligament and ligamentum flavum.

Ossification takes place in the posterior longitudinal ligament and ligamentum flavum of the spine under certain conditions of unknown nature. Ossification of these ligaments has the following characteristics: (1) ectopic bone formation occurring within the spinal ligaments; (2) ossification accompanies ligamentous tissue hyperplasia and cell proliferation; (3) before ossification, fibrocartilaginous cell proliferation, calcification and tissue resorption with vascular ingrowth take place sequentially; (4) ossification of the ligament has a specific site of predilection and often occurs in combination with senile ankylosing vertebral hyperostosis (Forestier's disease) or diffuse idiopathic skeletal hyperostosis; and (5) ossification and symptom development are remarkably more frequent in the Japanese population. Recent studies revealed that bone morphogenetic proteins and transforming growth factor-beta played an important role in the matrix hyperplasia and ossification of the spinal ligament, and metabolic and genetic aberration often characterized patients suffering from this disorder.

Humans↗

Rapid decalcification using microwaves for in situ hybridization in skeletal tissues.

In situ hybridization histochemistry is the sole tool available for detecting the localization and expression of specific RNA on histological sections under various in vivo conditions. For this paper, we examined the effect of microwave exposure on the time needed for decalcification of skeletal tissues and on the preservation of sensitivity for hybridization signals. Our data show that the use of microwave decalcification reduces the decalcification period while preserving intense hybridization signals for mouse alpha1 chain of procollagen type I mRNA in osteogenic cells in bone. The use of microwave treatment to decalcify skeletal tissues may prevent delay in obtaining experimental results or the loss of signals during in situ hybridization.

Animals↗

Peripheral nerve palsies in victims of the Hanshin-Awaji earthquake.

On January 17, 1995, the Hanshin-Awaji earthquake directly hit a metropolis and destroyed approximately 120,000 houses. Many people were buried under the debris of the Japanese style wooden houses or were injured by falling furniture. Twenty-five patients (35 extremities) with earthquake associated peripheral nerve palsy were studied and followed up for 2 years. The characteristic feature of earthquake associated palsies was combined nerve palsy induced by prolonged compression. There were 11 extremities with brachial plexus palsy; two extremities with combined radial, median, and ulnar nerve palsy; two extremities with combined median and ulnar nerve palsy; two extremities with radial nerve palsy; two extremities with ulnar nerve palsy; 10 extremities with combined femoral and sciatic nerve palsy; one extremity with combined tibial and peroneal nerve palsy; and five extremities with compartment syndrome of the leg. Many peripheral nerve palsies were induced by nerve ischemia. Most patients had good spontaneous recovery. The prognosis of these palsies generally was good.

Adolescent↗

Cervical lesions related to the systemic progression in rheumatoid arthritis.

STUDY DESIGN: Cross-sectional study of cervical involvement in rheumatoid arthritis. OBJECTIVES: To clarify the correlation between the deterioration of cervical lesions and the systemic progression of rheumatoid arthritis. SUMMARY OF BACKGROUND DATA: The natural course of cervical lesions varies. To date, no systemic parameter has been clarified to predict the progression. METHODS: One hundred seventy-three patients with rheumatoid arthritis participated in this study. The authors studied the progression of cervical lesions and investigated the relation between the types of cervical subluxation at the end of study and the following four variables: the serum level of C-reactive protein, the number of joints with erosion, carpal height ratio, and disease subset (least erosive subset, more erosive subset, and mutilating disease subset). RESULTS: Of the 173 patients, 55 already had cervical subluxation before entering the study. During the follow-up period, 44 patients deteriorated radiographically, and 77 (45%) had cervical involvement, including involvement of upper cervical lesions in 65 patients, upper lesions combined with subaxial subluxation in 10, and subaxial subluxation alone in 2. The upper cervical subluxation progressed in the order of anterior atlantoaxial subluxation, atlantoaxial subluxation combined with vertical subluxation, and vertical subluxation alone. Deterioration of upper cervical lesion and occurrence of subaxial subluxation were closely correlated with an elevation of serum C-reactive protein level, an increase in the number of joints with erosion, and a decrease in the carpal height ratio. The incidence of cervical involvement and the extent of deterioration were different among the disease subsets. CONCLUSIONS: The serum level of C-reactive protein, the number of joints with erosion, and the carpal height ratio correlated closely with the extent of the cervical subluxation. The average C-reactive protein values during the follow-up period correlated with progression of the cervical lesions. The classification of rheumatoid disease subset was useful for predicting the terminal feature of the cervical lesions.

Adolescent↗

Switch of osteonectin and osteopontin mRNA expression in the process of cartilage-to-bone transition during fracture repair.

The process of cartilage-to-bone transition (CBT) is a key event for the achievement of rigid bone healing during fracture repair. Since mineralization of cartilaginous matrix is a prerequisite for the initiation of CBT, the genetic localization of mineralization-related bone matrix proteins in CBT was examined in this study. An in situ hybridization method used on decalcified sections with digoxigenin-11-UTP labelled probes identified the cellular localizations of these genes in CBT. Cessation of osteonectin mRNA together with induction of osteopontin mRNA in chondrocyte maturation was observed during the process of CBT in the fracture callus on day 12 after fracture; osteocalcin mRNA was absent in chondrocytes of the CBT area. Induction of osteopontin mRNA in maturated chondrocytes was followed by the expression of mRNAs for osteonectin, osteopontin and osteocalcin in osteogenic cells in the ossification front of CBT. The data suggest that the switch from osteonectin to osteopontin mRNA expression in chondrocyte maturation is one of the key events during CBT. Transcriptional disorders of the expression of these molecules may be linked to the failure of fracture repair, i.e. delayed or prevented hypertrophic osteosynthesis.

Animals↗

Long-term follow-up results of laminectomy for cervical myelopathy caused by ossification of the posterior longitudinal ligament.

OBJECT: This retrospective study was performed to assess the long-term results of cervical laminectomy in treating ossification of the posterior longitudinal ligament (OPLL) of the cervical spine. METHODS: The authors reviewed medical records in 44 of 52 patients who underwent cervical laminectomy between 1970 and 1985 (mean follow up 14.1 years). The neurological recovery rate after laminectomy was 44.2% after 1 year and 42.9% after 5 years. The surgical outcome was maintained after 5 years but worsened between 5 and 10 years postsurgery: the recovery rate at the last follow-up review was 32.8%. Using multivariate stepwise analysis, the preoperative factors that affected clinical results were found to be the age at operation, the severity of preexisting myelopathy, and a history of trauma. Late neurological deterioration was observed in 10 (23%) of 44 patients. The earliest deterioration occurred at 1 year and the latest was at 17 years postsurgery (mean 9.5 years). The most frequent cause of deterioration was trauma due to a fall (six patients), followed by ossification of the ligamentum flavum (three patients). Postoperative spread of the OPLL was noted in 70% of the patients, but it was clearly the cause of neurological deterioration in only one of them. After laminectomy, postoperative progression of kyphotic deformity was observed in 47% of patients, but these changes did not cause neurological deterioration. CONCLUSION: The authors recommend early surgical decompression for OPLL because the outcome is better for younger patients and for those with a higher score as measured by the Japanese Orthopedic Association's system.

Accidental Falls↗

In vivo direct gene transfer into articular cartilage by intraarticular injection mediated by HVJ (Sendai virus) and liposomes.

OBJECTIVE: To establish a system for efficient, direct in vivo gene transfer into joints. METHODS: A hemagglutinating virus of Japan (HVJ; Sendai virus)-liposome suspension containing SV40 large T antigen (SVT) gene was injected intraarticularly into knee joints of 6-week-old female Lewis rats. Rats were killed at various times for immunohistochemical analysis of the expression of SVT gene. RESULTS: The expression of SVT gene was detected immunohistochemically in chondrocytes in the superficial and middle zones of articular cartilage in the knee joints. The average percentage of SVT-positive cells was estimated to be approximately 30% on days 3, 7, 14, and 21 after transfection. Moreover, no pathologic change caused by HVJ-liposome injection was observed in the joints. CONCLUSION: The transfection frequency and stability of expression recognized in this study indicate the possibility of a strategy for treatment of joint disorders, including arthritis, using direct gene transfer.

Animals↗

Natural history of upper cervical lesions in rheumatoid arthritis.

The natural history of the upper cervical lesions in rheumatoid arthritis (RA) was investigated, based on a follow-up study of 79 patients. At the beginning of this study, anterior atlantoaxial subluxation (AAS) was found in 35 patients, AAS combined with vertical subluxation (VS) in 34, and VS alone in 10. During the follow-up period (mean, 6.4 years), these lesions deteriorated in 30 of the 79 patients. To evaluate the occipitoatlantoaxial relation, the angles between occiput and atlas (C0/C1 angle) and between atlas and axis (C1/C2 angle) were measured, and the correlation between these angles and deterioration of the lesions was investigated. The severity of RA was classified according to the "disease subset" advocated by one of us (T.O.): the least erosive subset (LES), the more erosive subset (MES), and the mutilating subset (MUD). There was a significant positive correlation between the C1/C2 angle and atlantodental interval (ADI) and a significant negative correlation between the C0/C1 angle and the ADI. This indicates that the atlas not only shifts forward but also slips down and forward at an incline from the axis. With an increase of the atlantal inclination, the anterior arch of atlas displaces in an anteroinferior direction, and VS combines with AAS. The development of associated VS lessens the amount of the ADI. At the advanced stage of the natural course, AAS is concealed, and VS alone is demonstrated. We concluded that the upper cervical lesion deteriorated in the order of AAS, AAS + VS, and VS alone. In patients classified as the LES subset, only AAS was found, and VS was never combined with AAS at the terminal stage. In patients in the MES, VS frequently combined with AAS. All patients who deteriorated to VS alone were ranked as MUD.

Adult↗

Neck and shoulder pain after laminoplasty. A noticeable complication.

STUDY DESIGN: The authors retrospectively analyzed the prevalence and features of neck and shoulder pain (axial symptoms) after anterior interbody fusion and laminoplasty in patients with cervical spondylotic myelopathy. OBJECTIVES: To reveal the difference in prevalence of postoperative axial symptoms between anterior interbody fusion and laminoplasty and to clarify the pathogenesis of axial symptoms after laminoplasty. SUMMARY OF BACKGROUND DATA: Outcome of the cervical surgery is evaluated on neurologic status alone; axial symptoms after laminoplasty rarely have been investigated. Such symptoms, however, are often severe enough to interfere with a person's daily activity. METHODS: Ninety-eight patients had surgery for their disability secondary to cervical spondylotic myelopathy. Of those patients, 72 had laminoplasty, and 26 had anterior interbody fusion. The presence or absence of axial symptoms was investigated before and after surgery. The duration, severity, and laterality of symptoms were also recorded. RESULTS: The prevalence of postoperative axial symptoms was significantly higher after laminoplasty than after anterior fusion (60% vs. 19%; P < 0.05). In 18 patients (25%) from the laminoplasty group, the chief complaints after surgery were related to axial symptoms for more than 3 months, whereas in the anterior fusion group, no patient reported having such severe pain after surgery. CONCLUSIONS: The prevalence and severity of axial symptoms after laminoplasty proved to be higher and more serious than has been believed. Such symptoms should be considered in the evaluation of the outcome of cervical spinal surgery.

Adult↗

Post-traumatic syringomyelia.

STUDY DESIGN: This study retrospectively analyzed patients who developed post-traumatic syringomyelia secondary to spinal cord injury. OBJECTIVES: To identify an indicator that would predict the outcome of surgical treatment for post-traumatic syringomyelia. SUMMARY OF BACKGROUND DATA: Syrinx-subarachnoid shunting was chosen as a surgical treatment for post-traumatic syringomyelia. No previous study has been published concerning magnetic resonance imaging findings' ability to predict surgical results before surgery. METHODS: Nine patients diagnosed by magnetic resonance imaging with post-traumatic syringomyelia were the subjects of this study. The magnetic resonance imaging findings and surgical results were analyzed. RESULTS: Neurologic deterioration was found in five patients. Ascending dissociated sensory disturbances and motor weakness were noticed to occur characteristically above the level of the spinal cord injury. The other four patients complained of a slight worsening of numbness without displaying neurologic deterioration. The five patients with neurologic deterioration were treated with a syrinx-subarachnoid shunting. Two of the five patients experienced sustained neurologic improvement after a midline myelotomy, which allowed the fluid within the syrinx to spout out and cause the expanded spinal cord to collapse. This was called a "high-pressure syrinx." In these two patients, the preoperative magnetic resonance imaging demonstrated a positive flow-void sign. On the other hand, drainage of the syrinx in the three patients with a negative flow-void sign did not collapse the expanded spinal cord, and the surgical results were considered fair. This was called a "low-pressure syrinx." CONCLUSIONS: Post-traumatic syringomyelia was classified into two types. A preoperative distinction could be made based on the presence or absence of the flow-void sign on a T2-weighted magnetic resonance image.

Adolescent↗

Expansive laminoplasty for cervical radiculomyelopathy due to soft disc herniation.

STUDY DESIGN: A comparative study was performed in patients with cervical radiculomyelopathy due to soft disc herniation to compare surgical results of laminoplasty with anterior discectomy and arthrodesis. OBJECTIVES: To determine whether posterior decompression without discectomy can be selected for cervical myelopathy due to cervical soft disc herniation. SUMMARY OF BACKGROUND DATA: Anterior cervical discectomy and arthrodesis has had acceptable surgical results, but a significant number of complications have been reported, especially those related to bone grafting. METHODS: Sixteen patients who received expansive laminoplasty and 17 patients who received anterior arthrodesis were studied. The mean value of the anteroposterior diameter of the spinal canal was 12.4 mm in the former and 12.9 mm in the latter. The mean follow-up was 3.1 years in the laminoplasty group, and 8.8 years in the anterior arthrodesis group. There was no statistical difference between the two groups in prognostic factors believed to affect surgical results. Neurologic results were evaluated with postoperative scores and recovery rates by methods previously described, and assessed by the Japanese Orthopaedic Association scoring system. RESULTS: The difference between the two groups in the recovery rate and final Japanese Orthopaedic Association score was not statistically significant. Surgical complications were more frequent in the anterior arthrodesis group than in the laminoplasty group. Additional surgeries were performed in three cases (18%) in the anterior arthrodesis group: posterior wiring for delayed union and kyphotic deformity in two cases, and laminoplasty for deterioration of myelopathy in one case. Regression of herniated cervical disc after laminoplasty was confirmed by computed tomography or magnetic resonance imaging in six of eight cases (75%). CONCLUSIONS: When the incidence of complications and the possibility of regression of herniated disc are taken into consideration, expansive laminoplasty can be selected for radiculomyelopathy due to cervical disc herniation.

Female↗

[The dorsal approach in degeneratively changed cervical spine].

The posterior approach to the cervical spine was the only method of access to the spinal canal until the anterior approach was developed by Robinson and Smith, and Cloward. With the accumulation of experience in posterior decompression for cervical spondylotic myelopathy (CSM), successful laminectomy was guaranteed only when lordotic alignment of the cervical spine, wide and extensive laminectomy for adequate posterior shift of the spinal cord, and stability of the spine were ensured after surgery. The thick scar formation occasionally seen subsequent to postlaminectomy hematoma could lead to an unfavorable outcome. The insertion of surgical instruments, such as a Kerrison rongeur or a curette, into the spinal canal without being aware of how narrow the canal is, or uneven decompression of the spinal cord during resection of the laminae can impinge on or distort the spinal cord and result in deterioration of neurological function. Several authors have pointed out that postoperative loss of neural function is a hazard of surgical intervention. Owing to the poor results of conventional laminectomy for cervical compression myelopathy related to the problems mentioned above, Kirita developed extensive simultaneous decompression laminectomy to avoid distortion of the spinal cord by the edges of the resected laminae. Hattori devised an expansive Z-shaped laminoplasty in which the posterior wall of the spinal canal was preserved by Z-plasty of the prepared laminae. This was an attempt to prevent the invasion of scar tissue, i.e., the so-called laminectomy membrane, which was believed to be a cause of late neurological regression. He also expected that the laminae reconstructed by Z-plasty would provide support for the spine. The introduction of high-speed air-drills allowed successful development of this procedure. In 1977, Hirabayashi introduced an epoch-making laminoplasty, the expansive open-door laminoplasty. He described the advantages of this procedure as: possibility of decompressing multiple levels of the spinal cord simultaneously, better postoperative support of the neck, allowing earlier mobilization of the patients, prevention of postoperative kyphotic deformity of the cervical spine, and reduced mobility of the cervical spine postoperatively, which helps to prevent late neurological deterioration and progression of OPLL. Subsequent to the Hirabayashi laminoplasty, various modifications and supplementary procedures have been devised for further improvement of the safety and efficacy of decompression, and for improved stability of the spine. Aims, advantages and disadvantages of laminoplasty: The aims of the laminoplasty are to expand the spinal canal, to secure spinal stability and to spare the protective function of the spine. Preservation of mobility of the spine is also a goal of this procedure for multiple level involvement. Decompression can be extended along the nerve root by facetectomy. Preservation of the posterior spinal structures permits reinsertion of the nuchal muscles and the spinal ligaments after they have been totally or partially detached. This prevents kyphosis or listhesis of the cervical spine, which often develops after laminectomy, particularly in subjects below 50 years of age. Reconstructive procedures for reattaching muscles and/or ligaments to the spinous processes are added to the laminoplasty, improving the dynamic or ligamentous stability of the spine.

Cervical Vertebrae↗

Vertebral body replacement with a ceramic prosthesis for metastatic spinal tumors.

STUDY DESIGN: This study retrospectively analyzed the clinical outcome of vertebral replacement surgery with our unique ceramic prosthesis for spinal metastases. OBJECTIVES: To indicate the results of vertebral replacement surgery with a ceramic prosthesis. SUMMARY OF BACKGROUND DATA: Spinal metastasis often involves the vertebral bodies, of which abnormal fracture causes intractable pain and paresis. For such conditions, laminectomy or irradiation can have no effect because they do not improve spinal stability. Autogenous bone or bone cement are not durable materials, and fatigue fracture of the implanted material occasionally occurs. We developed a simple prosthesis made of alumina ceramic, a bioinert material, to replace the affected vertebrae. There has not been such a large study to assess vertebral replacement surgery with a ceramic prosthesis. METHODS: From 1972 to 1993, 90 ceramic prostheses were used in 84 patients with spinal metastasis, and the average follow-up period was 26.2 months. The primary tumor was thyroid cancer in 13 patients, breast cancer in 12, multiple myeloma in eight, renal cell cancer in eight, gastrointestinal cancer in eight, and lung cancer in eight. The clinical symptoms were assessed before and after surgery, and the maintenance of operative gain was investigated. RESULTS: Pain relief was achieved in 94%, motor function improved in 81%, and ambulation recovered in 64%. There were no serious complications associated with the procedure, and the operative benefit was maintained until the terminal stage in the vast majority of patients. CONCLUSIONS: In selected patients, vertebral replacement using our prosthesis proved to be a useful procedure, effectively managing the severe spinal pain or neurologic deficits associated with vertebral body destruction.

Adolescent↗

Intramedullary changes of the spinal cord in cervical spondylotic myelopathy.

STUDY DESIGN: This study retrospectively reviewed magnetic resonance imaging and delayed computed tomography after myelography of cervical spondylotic myelopathy patients who needed surgical treatment. OBJECTIVES: The purpose of this study is to clarify the meaning of high magnetic resonance intensity areas in cervical spondylotic myelopathy patients. SUMMARY OF BACKGROUND DATA: There is no conclusion whether the high magnetic resonance signal intensity areas can be a predictor for surgical results or not. METHODS: Thirty-one patients with cervical spondylotic myelopathy were examined with magnetic resonance imaging before surgery and delayed computed tomography after surgery. The presence or absence of high intensity areas in the spinal cord was compared with clinical symptoms and surgical outcomes. RESULTS: Twenty-three (74%) of 31 patients showed high intensity areas in the spinal cord on the T2-weighted image. Among these 23 patients, 18 revealed bilateral intramedullary "snake eyes" enhancement in delayed computed tomography. The presence of high intensity areas did not correlate with the surgical outcomes. Patients with multisegmental (linear) high intensity areas frequently manifested muscle atrophy in upper extremities. CONCLUSIONS: High intensity areas on T2-weighted magnetic resonance imaging were not correlated with the severity of myelopathy or surgical outcomes evaluated by the Japanese Orthopaedic Association score. Magnetic resonance imaging or delayed computed tomography in this study could not rule out the presence of white matter changes, including axonal loss or demyelination. Multisegmental (linear) high intensity areas on T2-weighted magnetic resonance imaging were associated with clinical evidence of extensive anterior horn cell and radiographic evidence of gray matter cavitation.

Aged↗

Postoperative cervical pseudomeningocele with herniation of the spinal cord.

STUDY DESIGN: This is a report of a patient in whom spinal cord herniation into a pseudomeningocele resulted in progressive myelopathy. OBJECTIVE: To describe the outcome of a 59-year-old man who visited Osaka University Hospital complaining of gait disturbance. He had undergone cervical laminectomy to resect a spinal cord tumor 14 years previously. SUMMARY OF BACKGROUND DATA: Pseudomeningocele is an extremely rare condition and can be overlooked. In addition, cord herniation into the pseudomeningocele rarely can be diagnosed before surgical exploration. Our patient represents the first case we are aware of in which magnetic resonance imaging could clearly demonstrate not only the pseudomeningocele, but the herniation of the cord into the cyst. METHODS: Magnetic resonance imaging was used for preoperative and postoperative investigation. RESULTS: The pseudomeningocele was resected to improve the neurologic status of the patient. During the operation, the herniated cord was successfully reduced into the original subarachnoid space by the release of adhesion. Most symptoms subsided soon after surgery. Magnetic resonance imaging could delineate not only the cyst and cord herniation, but the medullary pathology. The distribution of high-intensity areas on T2-weighted images suggested the cord damage. CONCLUSION: Magnetic resonance imaging revealed not only the cyst and cord herniation, but medullary pathology, too.

Hernia↗

Distribution of the basic fibroblast growth factor and its receptor gene expression in normal and degenerated rat intervertebral discs.

STUDY DESIGN: Using a rat spondylosis model, the distributions of cells expressing the basic fibroblast growth factor and its receptor were investigated in normal and degenerated intervertebral discs. Cell-proliferating activity in degenerated discs was also assessed. OBJECTIVES: This study was conducted to determine whether basic fibroblast growth factor is related to intervertebral disc degeneration. SUMMARY OF BACKGROUND DATA: Basic fibroblast growth factor stimulates proliferation and matrix synthesis of cultured intervertebral disc cells. METHODS: Immunohistochemistry and in situ hybridization histochemistry were conducted to detect cells with basic fibroblast growth factor-like immunoreactivity and fibroblast growth factor receptor messenger RNA, respectively. Cell-proliferating activity was evaluated by AgNOR staining. RESULTS: In degenerated discs, round chondrocytes with basic fibroblast growth factor-like immunoreactivity and fibroblast growth factor receptor messenger RNA are scattered instead of spindle-shaped cells in the normal anulus (normal anular cells), which are devoid of basic fibroblast growth factor-like immunoreactivity and fibroblast growth factor receptor messenger RNA. The proliferating activity of these chondrocytes is suggested to exceed that of normal anular cells. CONCLUSION: Basic fibroblast growth factor is suggested to promote proliferation of chondrocytes in degenerated discs in an autocrine or paracrine manner. Basic fibroblast growth factor may be related to intervertebral disc degeneration as a proliferation-stimulating factor of chondrocytes that replace normal anular cells during disc degeneration.

Animals↗