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Biomedical subjects

K Yoda

Publications and source records attributed to K Yoda.

At least 91 records · Page 5Linked to original sources
PubMed1990

Molecular cloning of the protocatechuate 4,5-dioxygenase genes of Pseudomonas paucimobilis.

We determined the nucleotide sequence of a 1.9-kilobase fragment of Pseudomonas paucimobilis SYK6 chromosomal DNA that included genes encoding protocatechuate 4,5-dioxygenase, the enzyme responsible for the aromatic ring fission of protocatechuate. Two open reading frames of 417 and 906 base pairs were found that had no homology with previously reported sequences, including those encoding protocatechuate 3,4-dioxygenase. Since both open reading frames were indispensable for the enzyme activity, they should encode the subunits of protocatechuate 4,5-dioxygenase. We named these genes ligA and ligB. Protocatechuate 4,5-dioxygenase was efficiently expressed in Escherichia coli with the aid of the lac promoter, and the polypeptides of the ligA and ligB gene products were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and amino acid sequencing.

Amino Acid Sequence↗
PubMed1989

A unique structure of a mouse gamma-actin processed pseudogene.

We have isolated several gamma-actin-related genes from a mouse genomic library. One of these has been shown to be a gamma-actin processed pseudogene (Tokunga, K., Yoda, K. and Sakiyama, S. (1985) Nucleic Acids Res. 13, 3031-3042). Here, we report the structure of another pseudogene (pMA131). pMA131 contained the sequences corresponding to the carboxyl half of a cytoskeletal actin in which random point mutations as well as insertion and deletion events took place. This region was flanked at its 5' end by the sequences related to mouse repetitive sequences, including the MIF-1 family, and was interrupted by the sequence homologous to the R family which is also a mouse repetitive sequence. The coding region was followed by the sequence corresponding to 3' untranslated region of gamma-actin mRNA.

Actins↗
PubMed1989

Molecular cloning of the structural gene for alkaline elastase YaB, a new subtilisin produced by an alkalophilic Bacillus strain.

Alkaline elastase YaB is an extracellular serine protease of the alkalophilic Bacillus strain YaB. We cloned the structural gene, ale, and determined the nucleotide sequence. The mature enzyme (268 amino acids) was preceded by a putative signal sequence and a prosequence (27 and 83 amino acids, respectively). The mature enzyme was 55% homologous to subtilisin BPN'. Almost all the positively charged residues are predicted to be on the surface of the molecule, which would facilitate binding to elastin. The P1 substrate site-related sequences differed between alkaline elastase YaB and subtilisin BPN'.

Amino Acid Sequence↗
PubMed1989

[Perioperative changes in cell-mediated immunity in patients with chronic renal failure].

The effect of anesthesia and surgery on cell-mediated immunity was investigated in patients with chronic renal failure (CRF) having latent immunological abnormalities compared with patients with normal renal function (NRF) as control. The patient had minor surgery with enflurane anesthesia. During the perioperative period the numbers of peripheral lymphocyte and its subsets were measured by single and two-color analysis, by applying monoclonal antibodies against lymphocyte membrane surface markers. While the number of peripheral lymphocyte was lower in CRF group than in NRF group throughout the whole period, it decreased significantly on the first and third post-operative days in CRF group. No change in peripheral lymphocyte subsets and CD 4/CD 8 ratio was observed except for increase in CD 8 one hour after incision and decrease in CD 8 on the first postoperative day. Significant difference from NRF group was observed neither in peripheral lymphocyte subset nor in CD 4/CD 8 ratio. In minor surgery under enflurane anesthesia no disturbance on immunological system was observed even in the patients with chronic renal failure.

Adult↗
PubMed1989

[Prostaglandin E1, as a vasodilator therapy, for low output syndrome after cardiac surgery].

Prostaglandin E1 (PGE1) has a direct vasodilating effect to smooth muscles of resistant vessel much more than that of capacitance one. In afterload mismatch, vasodilator which acts on resistant vessel mainly should be selected. We used PGE1 for vasodilator therapy to two patients with low cardiac output syndrome (LOS) in intensive care unit. One patient undergoing coronary artery bypass grafting developed LOS and disseminated intravascular coagulation which derived from post-transfusional anaphylactoid reaction. Another patient undergoing mitral valve replacement, tricuspid valve annuloplasty and coronary artery bypass grafting also developed LOS with massive thrombosis caused by cold agglutinin during cardio-pulmonary bypass. In both patients, PGE1 decreased systemic vascular resistance index and pulmonary arterial resistance index, and increased cardiac output. PGE1 inhibits release of lysosomal enzymes from polymorphonuclear leukocytes and increases splanchnic blood flow. These effects lead to inhibit production of myocardial depressant factor and might relate to the improvement of LOS partially. We suggest that PGE1 was effective for LOS after cardiac surgery, especially in afterload mismatch.

Aged↗
PubMed1989

[The use of PGE1 in an elderly patient with mitral valve regurgitation during general anesthesia].

PGE1 has a beneficial effect on cardiac failure with mitral valve regurgitation by decreasing the "after load". An 82-year-old female had a total cystectomy of a bladder tumor. The preoperative standard 12 lead electrocardiogram showed atrial fibrillation and incomplete right bundle branch block. The preoperative echocardiogram showed regurgitation of both mitral valve and tricuspid valve. Under heavy premedication, we intubated with fentanyl and pancuronium bromide, maintained anesthesia with enflurane. After incision, both pulmonary artery pressure and pulmonary capillary wedge pressure increased, and cardiac index decreased. Continuous injection of 100ng.kg-1.min-1 PGE1 made pulmonary artery pressure and pulmonary capillary wedge pressure to decrease, and cardiac index to increase. PaO2, however, decreased apparently. PGE1 was effective for cardiac failure with mitral valve regurgitation associated with pulmonary hypertension. But attention must be given to the decrease in PaO2.

Aged↗
PubMed1989

[Afterload reduction therapy with prostaglandin E1 (PGE1)].

Increases in afterload cause marked decreases in cardiac output and left ventricular stroke volume in patients with congestive heart failure. We studied the effects of afterload reduction therapy with prostaglandin E1 in three patients with intraoperative heart failure and two patients with low output syndrome after coronary artery bypass grafting. After 120 minutes, mean pulmonary arterial pressure decreased from 25.6 to 17.4 mmHg, pulmonary wedge pressure from 20.0 to 10.2 mmHg and right atrial pressure from 10.2 to 6.8 mmHg. Cardiac index increased from 1.73 to 2.80 l.min-1.m-2. The improvement of peripheral circulatory failure was remarkable and all patients recovered from heart failure. This study suggests that PGE1 is effective for afterload reduction therapy.

Aged↗
PubMed1989

[Anesthetic management of spinal progressive muscle atrophy (SPMA)].

The patient with spinal progressive muscle atrophy (SPMA) has many anesthetic problems; muscle atrophy, respiratory complications, hypersensitivity to non-depolarizing muscle relaxants and succinylcholine-induced hyperkalemia. Although neurological complications following spinal and epidural anesthesia have been reported in various neurologic diseases, we chose epidural anesthesia for the patient with SPMA and experienced no problems during and after the operation. We conclude that epidural anesthesia is a useful technique for the patient with SPMA.

Adult↗
PubMed1989

[Painless delivery under lumbar epidural anesthesia for a pregnant woman with syncope caused by paroxysmal ventricular tachycardia].

A 32-year-old pregnant woman complained of palpitation and syncope caused by paroxysmal ventricular tachycardia. The electrophysiological study was normal. The evidence that catecholamines were extremely increased in urine suggested that this arrhythmia resulted from imbalance in the autonomic nervous system. Medication by beta-blocking drugs during pregnancy decreased the frequency of ventricular tachycardia. In order to avoid the increase of catecholamines during the delivery, lumbar epidural anesthesia was administered, which resulted in painless vaginal delivery and impeded the attack of ventricular tachycardia. Although the baby was small for date, fortunately he was delivered normally.

Adult↗
PubMed1989

[Anesthetic management of thrombotic thrombocytopenic purpura].

A 46-year-old man, with TTP and renal failure, received splenectomy because complete remission was not achieved after the treatments such as plasma exchange, antiplatelet agents and corticosteroid. After the administration of methylprednisolone, anesthesia was induced with thiamylal and succinylcholine chloride and maintained with nitrous oxide, enflurane, fentanyl and vecuronium. Total blood loss was 1710 gram. Four units of packed red cell and 9 units of fresh frozen plasma were infused. The anesthetic course was uneventful. TTP is a rare and usually fatal disease and its etiology is unknown. Careless infusion of platelet or fresh blood may accelerate the disease process and multiorgan dysfunction may present other anesthetic problems. In this report, problems of anesthetic management for TTP were discussed.

Anesthesia, Inhalation↗
PubMed1989

[Dynamic local cerebral circulation study in patients with cerebral diseases--using 99mTc-HSA-D mainly compared with 99mTc-HSA].

We have been using 99mTc-RBC and 99mTc-HSA for cerebral angiography and measurement of the local cerebral blood volume (LCBV) and cerebral/peripheral hematocrit ratio (alpha). 99mTc-HSA, however, has a problem of poor stability in the blood. We conducted basic and clinical studies on 99mTc-HSA-D, a ready-to-use preparation with good stability in the blood. We measured the regional cerebral circulation dynamics in patients with cerebral diseases in comparison with 99mTc-RBC and 99mTc-HSA. 99mTc-HSA-D and 99mTc-RBC showed more than a 90% retention ratio in the blood 60 min after administration, and good SPECT images were obtained using these two pharmaceuticals. On the contrary, 99mTc-HSA was unstable and its retention ratio was 60s% 60 min after administration. 99mTc-HSA does not provide good SPECT images due to free 99mTcO4- which seems to be liberated from 99mTc-HSA in the body, etc. There were no adverse reactions or abnormal laboratory findings caused by the 99mTc-HSA-D administration in any of the patients. Based on these results, it is surmised that 99mTc-HSA-D is clinically useful for measurement of the cerebral blood volume and cerebral/peripheral hematocrit ratio (alpha).

Adult↗
PubMed1988

RNA-primed initiation sites of DNA replication in the origin region of bacteriophage lambda genome.

Using DNA molecules synthesized in the early stage of lambda phage infection, deoxynucleotides at the transition sites from primer RNA to DNA synthesis have been mapped in the 1.5 kbase area of the lambda phage genome containing the genetically defined replication origin (ori lambda). Sites in the 1-strand (the polarity of the 1-strand is 5' to 3' from the left to the right direction of the lambda phage genetic map) were distributed both inside and outside of the ori lambda, whereas the sites in the r-strand (the strand in the opposite polarity) were mainly distributed more than three hundred nucleotides apart from the ori lambda to the right. A CPuPu sequence was found at -12 to -10 region of transition sites of the r- and the 1-strands in the frequency of 80% and 70%, respectively, and over 60% of the CPuPu sequences were CAG. Properties of the transition sites are discussed in relation to the primer synthesis.

Bacteriophage lambda↗
PubMed1988

Designed gene amplification on the Bacillus subtilis chromosome.

We previously reported the cloning of a 1.6 kb HindIII fragment (containing the junction of the repeating unit) from chromosomal DNA of Bacillus subtilis strain B7 in which tandem amplification of a 16 kb region occurred, and the induction of B7-type gene amplification by competence transformation with this cloned fragment. Based on this result, we designed, on the B. subtilis chromosome, a gene amplification of the 22 kb repeating unit containing the alpha-amylase structural gene (amyE), the tunicamycin-resistance gene (tmrB) and the shikimate kinase structural gene (aroI). We cloned only two short DNA fragments from both termini of the 22 kb region, constructed a junction structure of the designed repeating unit on pBR327 and transformed a B. subtilis wild-type strain by this constructed plasmid. As a result, we succeeded in obtaining tunicamycin-resistant (Tmr) transformants in which the designed gene amplification of 22 kb occurred on the chromosome. The Tmr transformants showed high productivity of alpha-amylase and shikimate kinase. The copy number of the repeating unit was estimated to be 10-20. This system may provide an effective means of amplifying long (greater than 20 kb) DNA regions on the chromosome.

Amylases↗
PubMed1988

[The sensitized rate of preformed antibodies in the candidate of renal transplant recipient].

It is important to examine the presence of preformed antibodies in hemodialysis patients before renal transplantation, because it causes the graft rejection in organ transplantation. In the 239 hemodialysis patients we analysed 3 preformed antibodies divided TW, BW and BC. Sensitized rate (TW+, BW+ and BC+) was 0% at no blood transfusions (BT), 9.3% at 1-5 BT and 28.9% at 6 more BT. The number of blood transfusions increased the sensitized rate (TW). Sensitized rate (BW+, BC+) was 6.2% at no BT, 9.3% at 1-5BT and 12.0% at 6 more BT, while sensitized rate (BC+) was 33.3% at no BT, 26.7% at 1-5BT and 14.5% at 6 more BT. There was no correlation between the number of blood transfusions and the sensitized rate (BW+, BC+). At one year after the first check, 69.2% of TW positive patients was converted to TW negative and 53.8% of BW positive patients was converted to BW negative each. Further studies of the preformed antibodies are required to assess the clinical course of long surviving patients.

Adolescent↗