Biomedical subjects

K Wiktorowicz

Publications and source records attributed to K Wiktorowicz.

15 recordsLinked to original sources

PubMed1992

Phenotype of lymphocyte in ovarian tumor peritoneal fluid. A preliminary study.

Phenotype of lymphocytes was estimated in 16 peritoneal fluids obtained from 9 ovarian cancer patients. Peritoneal fluids contained predominantly T cells (more than 80%) while B cell content was relatively low. Approximately 50% lymphocytes carried T-helper cell marker (CD4). Percentage of T-cytotoxic/suppressor cells (CD8+ cells) differed between patients with progression and those showing no tumor progression (19.0 +/- 7.5 vs 32.7 +/- 18.5). CD4/CD8 ratio was higher in the group of patients with progression than in the group with no progression of the tumor (3.4 +/- 2.1 and 2.0 +/- 1.5). Chemotherapy did not affect the parameters. Estimation of CD8 and CD4/CD8 ratio in peritoneal fluid cells could be helpful in monitoring of disease progression.

Adult↗

PubMed1992

Comparative studies on in vitro mitogen-induced proliferation of peripheral blood lymphocytes in dog and breeding fox.

In vitro blastogenesis of dog and fox lymphocytes was compared by a microculture technique. The highest 3H-thymidine incorporation in cultures of dog lymphocytes was observed at day 3, while in those of fox at day 2, incubated either at 37 degrees C or at 39 degrees C. Lymphocytes cultured at 39 degrees C incorporated more tritiated thymidine than did cells cultured at 37 degrees C. The stimulation index (SI) of dog peripheral blood lymphocytes to both mitogens concanavalin A (Con A) and leucoagglutinin (LA) was in a similar range, while pokeweed mitogen (PWM) showed a weaker but significant stimulatory action. The blastogenesis of fox lymphocytes was the greatest in Con A stimulated cultures. The mitogenic potency of LA and PWM was about half of that of Con A, with no essential difference between them. Maximum lymphocyte proliferation of dog and fox was observed when culture media were supplemented with 10% fetal calf serum (FCS).

Animals↗

PubMed1991

Optimal conditions for in vitro mitogen-induced proliferation of peripheral blood lymphocytes in breeding foxes.

The proliferative response of fox peripheral blood lymphocytes to nonspecific mitogens: leucoagglutinin (LA), concanavalin A (Con A) and pokeweed mitogen (PWM) was studied. Microcultures were kept at 39 degrees C in a humidified atmosphere containing 5% CO2. The highest 3H-thymidine incorporation was observed, when Con A was used, while LA and PWM showed weaker but significant stimulatory action. Optimal doses of mitogens were: 5 micrograms/ml for Con A, 5 micrograms/ml for LA and a dilution of 1:100 for PWM. The maximal stimulation index for Con A was about 240 and up to 100 for LA or PWM. The maximal lymphocyte proliferation was observed when culture media were supplemented with 10% serum. When proliferation kinetics were studied, the peak response was observed on Day 2.

Animals↗

PubMed1991

Binding of C-reactive protein (CRP) by human peripheral blood lymphocytes in vivo.

Antigenicity of C-reactive protein (CRP) on the surface of human lymphocytes was investigated by use of indirect immunofluorescence technique with anti-CRP antibodies. CRP on the lymphocyte surface (sd-CRP) belongs to two different categories: i) CRP produced by lymphocytes and inserted into cell membrane (s-CRP), ii) CRP produced primarily by the liver and bound by the lymphocytes (sb-CRP) in calcium-dependent manner. In human peripheral blood of healthy donors approximately 2.5% of lymphocytes expressed membrane CRP (s-CRP) and 1.5% of lymphocytes bound CRP in calcium-dependent manner (sb-CRP). Percentage of s-CRP lymphocytes increased in patients with rheumatoid arthritis, while population of sb-CRP lymphocytes did not change significantly, except cases where serum CRP concentration reached more than 50 micrograms/ml. Thus, it can be concluded that CRP is bound to the distinct population of lymphocytes, bearing specific membrane receptors.

Antigens, Surface↗

PubMed1987

Microheterogeneity forms of alpha 1-acid glycoprotein as indicators of rheumatoid arthritis activity.

The microheterogeneity of alpha 1-acid glycoprotein (AGP) has been studied in the sera of 48 patients with rheumatoid arthritis and of 12 healthy individuals. For each rheumatoid patient the disease activity has been assessed and each patient has been assigned to one of four activity grades: I, inactive; II, mildly active; III, moderately active; and IV, severe. Concanavalin A-affinity electrophoresis revealed three microheterogeneity variants of AGP: non-reactive with Con A, weakly reactive with Con A and strongly reactive with Con A. The relative amounts of AGP-variants observed in the healthy donors were similar to those observed in the patients with activity grade I, but differed significantly from patients with grades II, III and IV. The differences between the AGP-patterns of patients with activity grades II, III and IV were also statistically significant.

Arthritis, Rheumatoid↗

PubMed1986

PHA responsiveness and adherent cell-lymphocyte interactions in patients with advanced malignant tumors of the ovary prior to treatment and during chemotherapy.

DNA synthesis in cultures of PHA-stimulated blood mononuclear cells from patients with advanced malignant tumors of the ovary before treatment was significantly reduced and responses of lymphocytes alone were, on an average, 40% lower than in control subjects. Adherent cells were found to suppress the PHA response of autologous lymphocytes from 6/12 patients before treatment, 4/18 patients during chemotherapy and only in 2/19 control subjects. The net effects of adherent cell-lymphocyte interactions in autogeneic vs. allogeneic combinations were frequently discordant, which suggests shifts in lymphocyte subpopulations responsive, respectively, to suppressor- and helper-type mediators released by monocytes in patients. PHA responses of mononuclear blood cells from patients after the 1st and 2nd round of chemotherapy were usually higher than the mean in untreated patients, but declined rapidly after subsequent rounds of cytostatic drug treatment and were deeply impaired in a few cured patients examined many months after therapy. Previous radiotherapy appeared to have little effect on the magnitude and patterns of changes of blood mononuclear response to PHA during subsequent chemotherapy.

Adult↗

PubMed1985

Lectin inhibition system for determination of concanavalin A glycoprotein complexes dissociation constants in agarose affinity electrophoresis.

A new system for lectin-glycoprotein complexes dissociation constants (K) determination is presented. The system is based on agarose affinity electrophoresis where equal lectin (Con A) concentrations are inhibited by variable specific sugar (alpha-methyl-mannoside) amounts. Moreover, the system allows lectin-sugar inhibition constants (Ki) studies. For determination of mechanisms as well as K and Ki values calculations mathematical equations are developed. Values of K for two variants of alpha 1 acid-glycoprotein, two variants of alpha 1-antitrypsin, one variant of alphafetoprotein and Ki for Con A-alpha-MM are calculated according to the introduced equations and compared in a computed system. Moreover, the influence of sugar on lectin-glycoproteins interaction is demonstrated and discussed.

Arthritis, Rheumatoid↗

PubMed1985

Comparison of three immunoassays for C-reactive protein determination.

Three widely used immunoassays for C-reactive protein (CRP) determination (RID. RIEP and LN--Behring) were compared. According to the CRP calcium dependent structural changes or ligand binding properties two buffer systems, Ca2+ rich and Ca2+ depleted, were used in all methods. In RID and RIEP mean values of studied samples were higher when Ca2+ depleted buffer was used (r = 0.94 and r = 0.88, respectively). However, in LN mean values of studied samples were higher when Ca2+ rich buffer was employed (r = 0.923). Comparison of RID and RIEP showed better correlation when Ca2+ depleted buffer was used with r = 0.913 and regression equation y = 0.92 chi + 2.76. Comparison of RID with LN gave similar results as in the case of RIEP and LN. In both cases mean values of studied samples were much higher in LN. Best correlation between RID and LN was obtained when RID was run in Ca2+ depleted buffer and LN performed in Ca2+ rich buffer (r = 0.845, y = 1.25 chi + 18.4). When RIEP and LN were compared highest correlation was obtained when Ca2+ rich buffer in both methods had been used (r = 0.84, y = 1.45 chi + 33.7). However, comparison of both methods run in Ca2+ depleted buffer yielded better regression equation: y = 1.05 chi + 26.1 with r = 0.795.

Arthritis, Rheumatoid↗

PubMed1985

The effect of C-reactive protein on the PHA-induced proliferation of human peripheral blood lymphocytes.

The effect of CRP purified from ascites fluid originated from cancer patients on human peripheral blood lymphocytes was investigated. CRP was purified using 4-step procedure including absorption on Sepharose 4B, phosphocholine-Sepharose, DE-52 ion exchange chromatography and gel filtration on Sephacryl S-200. CRP was able either to inhibit or to enhance PHA-induced lymphocyte proliferation in vitro. The effects of added CRP depended on the time of its addition, dose of CRP and lymphocyte donor.

C-Reactive Protein↗

PubMed1979