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Biomedical subjects

K Włodarski

Publications and source records attributed to K Włodarski.

At least 19 recordsLinked to original sources
PubMed2000

Perichondrial chondrogenesis and periosteal osteogenesis by localized graft-vs-host reaction in mice.

Localized GvH reaction produced by the subcutaneous injection of parental lymphoid cells into the ear lobe of F1 recipient mice results in local activation of perichondrial chondrogenesis. Similarly, when the GvH reaction was elicited in shanks, the periosteal membranes at the site of immune reaction were stimulated to proliferate and to produce new bone. However, syngeneic lymphocytes activated in vivo by Con A and untreated syngeneic spleen and bone marrow cells administered locally produced similar response of bone and cartilage. Thus the lymphocytes activated either in the course of GvH reaction or syngeneic lymphocytes grafted into heterotopic sites presumably release mediators capable to stimulate periosteal/perichondrial membranes.

Animals↗
PubMed1999

Local hypertrophic/hyperplastic changes of keratinizing squamous epithelium of pinna induced by concanavalin A and other immunomodulators in mice.

Intradermal administration of concanavalin A, a potent T-cell mitogen, into an ear lap resulted in activation of chondrogenesis and stimulation of epidermis proliferation. This proliferation is sometimes invasive in character (pearls and epidermal nests form in the underlying connective tissue) but never turns into true cancerous lesions. This reaction can be delayed, but not prevented, by the prostaglandin inhibitor indomethacin. Stimulation of epidermis proliferation was also caused by administration of other immunomodulators, such as carrageenan type IV, Moloney sarcoma development, and rarely in the course of GvHr, but to much lesser degree than with concanavalin A. It is suggested that the same growth factors, which are mediators of local chondrocyte stimulation, are also mediators of keratinocyte activation.

Adjuvants, Immunologic↗
PubMed1993

Results of the administration of bisphosphonates for the prevention of periosteal bone formation following Moloney sarcoma virus-induced tumors.

The effect of continuous and discontinuous administration of bisphosphonates on the periosteal osteogenesis induced by Moloney sarcoma development was studied in mice by histological examination and bone dry mass evaluation. Both EHDP and Cl2MDP did not inhibit periosteal osteogenesis but EHDP, in contrast to Cl2MDP, inhibited the mineralization of osteoid. This inhibition was completely reversible after the withdrawal of the drug. Within two weeks the remineralization of newly formed bone matrix was completed.

Animals↗
PubMed1990

Murine lymph nodes in response to the local administration of dextran sulphate.

In mice a single injection of 4 mg of Dextran sulphate within a few days produces an enlargement of area draining lymph nodes and activates basophils/mast cells. This activation is manifested by many-fold increase of mast cell number per lymph node, degranulation of mast cells and appearance of young, immature mast cells capable to synthetize new granules. In contrast to the lymph nodes, the Dextran-injected connective tissue mast cells remained unchanged. This suggest that Dextran sulphate directly activates lymph node mast cells, probably by activation of T-cell suppressor or macrophages.

Animals↗
PubMed1985

Lymphatic mast cells in response to in vitro stimulation by non-specific T-cell mitogens.

Activation of lymph nodes by the T-cell mitogens PHA and Con A is correlated with a depletion of lymphatic mast cells. This result, and our earlier reports on the depletion of mast cells in lymph nodes stimulated by allogeneic and tumour cells, as well as on the elevation of mast cell number in thymus-less 'nude' mice, lead us to the conclusion that antigen- or mitogen-stimulated T lymphocytes produce lymphokine(s) which degranulates mast cells and/or is responsible for their negative chemotaxis.

Animals↗
PubMed1985

Transfusions of syngeneic blood do not seem to induce suppressive effect on antitumor or transplantation immunity in mice.

There was no prolongation of allogeneic heart survival or acceleration of tumor development in mice pretransfused with syngeneic blood. Analogous transfusion of donor-specific (allogeneic) blood, without additional immunosuppression, resulted in prolongation of allogeneic heart survival. These results argue against participation of some immunologically unspecific mechanisms in immunosuppressive effect of blood transfusion on transplantation immunity in this model.

Animals↗
PubMed1985

Does lymphopoiesis regulate tissue mast cells?

An almost twenty-fold increase in mast cell concentration was observed in the spleens of BALB/c mice after sublethal X-irradiation. Repopulation with bone marrow cells abolished this effect.

Animals↗
PubMed1985

Decrease of lymphatic mast cells in response to syngeneic WAMIB tumor cells.

In response to the syngeneic carcinoma cells the regional lymph nodes are enlarged, but their lymphatic Mast Cell content was reduced in both, relative and absolute terms. This result confirms our earlier data on the depletion of lymphatic Mast Cells in the lymph nodes stimulated by various antigens.

Animals↗
PubMed1984

Induction of heterotopic and orthotopic cartilage and bone formation in mice.

Heterotopic cartilage, bone and bone-marrow formation was achieved in mice by transplantation of a variety of xenogeneic established cell lines, by the transitional epithelium or by implants of demineralized bone matrix. The pattern and the sequence of events were always the same, regardless of the inducer used; viz., hyaline cartilage appeared 6-7 days after implantation, and endochondral bone formation followed. However, in cases of allogeneic implants of transitional epithelium into species other than the mouse, an intramembranous osteogenesis was the main mode of bone formation. When the yield of induced bone was high enough, a true myelopoiesis developed after three weeks. Heterotopically-induced bones had a relatively short life-span. Periosteal membranes of bones at the sites of sarcomes induced by M-MSV responded with rapid and extensive proliferation, with subsequent bone and, sometimes, hyaline cartilage deposition. This phenomenon was observed in long and cranial bones. However, bone induced heterotopically by demineralized bone matrix did not respond in such a way to the presence of M-MSV-induced sarcoma, suggesting that the connective tissue-encapsulated heterotopic bone was not a functioning periosteum. M-MSV-induced sarcoma also stimulates proliferation of elastic cartilage.

Animals↗
PubMed1983

Lymph node mast cells in athymic "nude" (Nu/Nu) and thymus containing (Nu/+ and +/+) Balb/c mice.

Total number of mast cells in popliteal lymph nodes of Balb/c mice is a function of time and Nu gene (complex?) content. The number of mast cells increases with age - in both wild +/+ and in Nu gene (complex?) carriers (Nu/+ and Nu/Nu). The increase in the number of mast cells is faster in the Nu gene carriers than in wild animals +/+ devoid of this gene. In "nude" mice having advanced hepatitis a dramatic increase in the number of lymphoid mast cells was observed. Results obtained deny the importance of thymus in the production of lymphatic mast cells in mice.

Age Factors↗
PubMed1983

Strain specific transplantable medullar carcinoma in DBA/2W mice.

A solid medullar carcinoma, which arose spontaneously in DBA/2W mouse was passed in vivo in syngeneic and semisyngeneic recipients for ten generations without alteration of its morphology. This cancer, which we named WAMIB, has a relatively slow growth ratio with MST 59 days and can be easily reproduced from cell cultures and/or from cryopreserved tumor fragments. WAMIB tumor is nearly diploid and does not metastasize, does not regress spontaneously and is rejected when grafted into allogeneic recipient animals.

Animals↗
PubMed1983

Decrease of mast cells in the stroma of human cancer.

The number of mast cells in the stroma of human squamous cell cancer -- keratoblastif cum, paraepidermale and male diffusa was 2-30 times lower than in the stroma o-normal squamous epithelium or in the connective tissue distant from neoplastic epithelium. These differences are statistically highly significant. The reduction of mast cel, number apparently might be the result of exhaustion of heparin-containing granules which interfere with tumor growth.

Aged↗
PubMed1981

Virally induced periosteal osteogenesis in mice.

Inoculation of Moloney murine sarcoma virus (M-MSV) into shank muscles of adult NMRI mice resulted in localized sarcoma and periosteal membrane proliferation with subsequent periosteal bone formation. Newly formed bone arose from outer surface of shank bones. Two weeks after M-MSV inoculation the width of bone cortex increased nearly 4 times. The spaces between newly formed bone ossicles were filled with bone marrow. In the later stages these bone marrow cavities were merged with medullar cavities. No extraskeletal bone formation was observed. The regression of M-MSV-induced sarcomas coincides with cessation of the proliferation of bone therefore allowing the maturation and rebuilding of bone. Newly formed bone was not resorbed during a 6-month observation period.

Animals↗