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Biomedical subjects

K W Mills

Publications and source records attributed to K W Mills.

26 records · Page 2Linked to original sources
PubMed1983

Use of enzyme-linked immunosorbent assay for detection of K88 pili in fecal specimens from swine.

Enterotoxigenic Escherichia coli are important in neonatal pig diarrhea. To be pathogenic, E coli must possess the ability to produce toxin and adherence structures called pili. An enzyme-linked immunosorbent assay was used to detect K88 pili in feces of swine. Results were compared with standard isolation and identification techniques. Isolates were tested for labile toxin production by the Y-1 mouse adrenal gland test and presence of pili by the enzyme-linked immunosorbent assay test. The ability to detect K88 pili in feces correlated with the isolation of labile toxin-positive, K88-positive E coli.

Animals↗
PubMed1982

Using enzyme-linked immunosorbent assay to detect Escherichia coli K88 pili antigens from clinical isolates.

Colonization of the small intestine is a prerequisite for enterotoxigenic Escherichia coli (ETEC) to cause diarrheal disease. Colonization is dependent on the capability of ETEC to adhere to the villous epithelium of the small intestine. This adherence attribute is conferred by pili structures produced by ETEC. The present study compares the efficiencies of the standard agglutination test, Y-1 mouse adrenal cell test, and infant-mouse gastric test with the efficiency of the enzyme-linked immunosorbent assay (ELISA) for the detection of the K88 pilus antigen and enterotoxin-producing E coli. The ELISA, a double antibody sandwich assay utilizing specific anti-K88 pilus antiserum, was used. Identification of isolates from clinical samples was accomplished on suspensions of bacteria. The sensitivity of the assay was in the nanogram per milliliter range, as determined by measuring purified pili. Results could be determined visually, but quantitative results indicated a positive optical density to negative optical density rate of 1.9 to greater than or equal to 3.0 on samples submitted to a clinical laboratory. The development of this assay indicates the application of such an ELISA for rapid identification of ETEC possessing K88 pili.

Agglutination Tests↗
PubMed1978

Serotyping herpes simplex virus isolated by enzyme-linked immunosorbent assays.

An enzyme-linked immunosorbent assay (ELISA) using a double-antibody sandwich tecnhique has been developed to serotype isolates of herpes simplex virus from clinical sources. The results obtained using this procedure were in agreement with those obtained with a standard neutralization test in typing stock cultures and 32 clinical isolates of herpes simplex virus. Clear differentiation between the two viral serotypes was obtained using rabbit immunoglobulin cross-absorbed with heterologous virus antigen. The ELISA procedure described appears to be a convenient and accurate substitute for the neutralization test in typing herpes simplex viruses. ELISA techniques require relatively small amounts of antigen and antibody and can be performed with very simple equipment.

Enzyme-Linked Immunosorbent Assay↗
PubMed1971

Do lymphocytes protect the rheumatoid joint?

The pathology of the synovial membrane in rheumatoid arthritis can be characterized by the prominence of two major features which appear to vary independently of one another. These are synovial lining-cell proliferation and infiltration with inflammatory cells, predominantly lymphocytes. In a series of 42 synovectomies from 36 patients it has been shown that extensive joint damage is associated with a synovial picture of marked lining-cell proliferation and a sparsity of lymphocytes. Cases with heavy lymphocyte infiltration tend to show less damage to articular cartilage and bone despite a similar duration of disease. These results have been compared with the picture in leprosy, where a similar pathological spectrum is associated with changes in cell-mediated immunity necessary for the control of infection.

Adolescent↗