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Biomedical subjects

K W King

Publications and source records attributed to K W King.

At least 19 recordsLinked to original sources
PubMed2005

Impact of soil amendments on reducing phosphorus losses from runoff in sod.

Received for publication December 22, 2004. Research was initiated to study the interaction between soil amendments (lime, gypsum, and ferrous sulfate) and dissolved molybdate reactive phosphorus [RP(<0.45)] losses from manure applications from concentrated runoff flow through a sod surface. Four run-over boxes (2.2-m2 surface area) were prepared for each treatment with a bermudagrass [Cynodon dactylon (L.) Pers.] sod surface (using sod blocks) and composted dairy manure was surface-applied at rates of 0, 4.5, 9, or 13.5 Mg ha-1. The three soil amendments were then applied to the boxes. Two 30-min runoff events were conducted and runoff water was collected at 10-min intervals and analyzed for RP(<0.45). Results indicated that the addition of ferrous sulfate was very effective at reducing the level of RP(<0.45). in runoff water, reducing RP(<0.45) from 1.3 mg L(-1) for the highest compost rate with no amendment to 0.2 mg L(-1) for the ferrous sulfate in the first 10 min of runoff. Lime and gypsum showed a small impact on reducing RP(<0.45), with a reduction in the first 10 min to 0.9 and 0.8 mg L(-1), respectively. The ferrous sulfate reduced the RP(<0.45) in the tank at the end of the first runoff event by 66.3% compared with no amendment. In the second runoff event, the ferrous sulfate was very effective at reducing RP(<0.45) in runoff, with no significant differences in RP(<0.45) with application of 13.5 Mg ha(-1) compost compared with no manure application. The results indicate that the addition of ferrous sulfate may greatly reduce RP(<0.45) losses in runoff and has considerable potential to be used on pasture, turfgrass, and filter strips to reduce the initial RP(<0.45) losses from manure application to the environment.

Animals↗
PubMed2000

Pyoderma gangrenosum in a patient with essential thrombocythemia.

BACKGROUND: Pyoderma gangrenosum is an uncommon ulcerative condition associated with inflammatory bowel disease, arthritis, and hematologic disease. We report a patient with essential thrombocythemia and pyoderma gangrenosum. OBJECTIVE: This article is a review of the associations between pyoderma gangrenosum and other diseases. RESULTS: There have been two previous reports of patients with pyoderma gangrenosum and essential thrombocythemia. CONCLUSION: There may be a possible association between pyoderma gangrenosum and essential thrombocythemia. The diagnosis of pyoderma gangrenosum should be considered in patients with essential thrombocythemia and cutaneous ulcers.

Anti-Inflammatory Agents↗
PubMed1999

Organisation of the mouse and human 5T4 oncofoetal leucine-rich glycoprotein genes and expression in foetal and adult murine tissues.

The human 5T4 oncotrophoblast leucine-rich glycoprotein may contribute to the process of placentation or metastasis by modulating cell adhesion, shape and motility. To understand better the role of 5T4 in development and cancer, the gene structure has been elucidated from both human and mouse genomic clones and mRNA expression has been studied in foetal and adult mouse tissues. The protein coding region is located within the second of two exons, the first exon comprising solely of 5'-untranslated region. Upstream there are no TATA or CAAT boxes, but there are a number of potential Sp1 binding sites. The murine and human proteins show a homologous domain organisation of the leucine rich repeats (LRR) and associated N- and C-terminal flanking regions, although the hydrophilic sequence which intervenes between the two LRR domains contains six additional amino acids in the mouse. The signal peptide, transmembrane region and cytoplasmic tail sequences are identical as are 6 out of the 7 potential N-linked glycosylation sites. Mouse 5T4 transcripts are abundant in placenta and also highly expressed in embryos while in adult tissues transcripts are restricted to brain and ovary. These patterns of expression and the genomic organisation are discussed in relation to possible function and other recently described LRR containing proteins.

Amino Acid Sequence↗
PubMed1997

Characterization of the gene encoding Mhp1 from Mycoplasma hyopneumoniae and examination of Mhp1's vaccine potential.

The gene encoding Mhp1, a 124 kDa protein from Mycoplasma hyopneumoniae, has been cloned, sequenced, and its product characterized. No significant homology to the gene or encoded polypeptide was found in the Genbank, NBRF, or PIR databases, though this protein appears similar to p97, a putative adhesin of M. hyopneumoniae described by Zhang et al. (Infect. Immun. 63, 1013-1019, 1995). Two repeated motifs were identified within the 3' end of the gene and encoded polypeptide. The mhp1 gene was fused to the glutathione S-transferase (GST) gene from Schistosoma japonicum, enabling high-level expression and purification of the protein. Both the authentic and recombinant proteins were recognized by sera from pigs infected with M. hyopneumoniae. In an induced-disease model in pigs, coughing was reduced in animals vaccinated with recombinant GST-Mhp1, although differences were not significant. Only minimal protection against lung lesion formation was provided, and again differences between the Mhpl-vaccinated and nonvaccinated groups were not significant.

Amino Acid Sequence↗
PubMed1994

Cloning and characterization of the recA genes from Mycoplasma pulmonis and M. mycoides subsp. mycoides.

The RecA protein has a central role in DNA repair and is essential for homologous recombination in most eubacteria. Little is known about these critical processes in mycoplasmas. By using standard and inverse polymerase chain reactions (PCR) coupled with conventional cloning techniques, a series of overlapping fragments comprising the entire recA genes of Mycoplasma mycoides subsp. mycoides (Mm) and Mycoplasma pulmonis (Mp) were generated. Each gene was sequenced in its entirety. The recA genes of Mm and Mp would encode proteins of 345 amino acids (aa) and 339 aa, respectively. The mycoplasmal RecA proteins revealed strong conservation when compared with RecA sequences from other bacterial species.

Amino Acid Sequence↗
PubMed1994

Mycoplasmal cloning vectors derived from plasmid pKMK1.

Only two plasmids have been isolated and characterized from the entire genus Mycoplasma, which includes over 90 recognized species. Both of these plasmids were obtained from the same species, Mycoplasma mycoides subsp. mycoides. We have previously characterized one of these plasmids, pKMK1, as a preliminary step in developing mycoplasmal cloning vectors. In the present study, we have separately combined pKMK1 with two different Escherichia coli replicons and a tetracycline resistance (tetM) gene. One of the constructs, plasmid p2D4, was shuttled from E. coli to M. mycoides subsp. mycoides and back to E. coli with no deletions or rearrangements occurring in the plasmid. In the second construct, the E. coli replicon was deleted when the plasmid was transformed into M. mycoides subsp. mycoides. This derivative, designated plasmid pIK delta, is noteworthy in that it could be transformed into M. mycoides subsp. mycoides at a much higher frequency than the parental plasmid. A gram-positive bacterial erythromycin resistance determinant (erm) was cloned into both p2D4 and pIK delta. Resistance to erythromycin was stably maintained using both constructs, even in the absence of erythromycin selection, indicating that these plasmids will be useful mycoplasmal cloning vectors.

Blotting, Southern↗
PubMed1994

Transformation of Mycoplasma capricolum and examination of DNA restriction modification in M. capricolum and Mycoplasma mycoides subsp. mycoides.

Plasmids pIK delta and pIK delta-erm have recently been developed as mycoplasmal cloning vectors. In this report, we demonstrate that these plasmids can replicate in Mycoplasma capricolum, a mycoplasmal species for which transformation had not previously been characterized. Both plasmids are stably maintained at a higher copy number than in their parental species, Mycoplasma mycoides subsp. mycoides. We have also examined the possibility of one or more restriction-modification systems affecting transformation frequencies in both species.

Cloning, Molecular↗
PubMed1992

Nucleotide sequence of Mycoplasma mycoides subspecies Mycoides plasmid pKMK1.

To facilitate the development of mycoplasmal cloning vectors, we have determined the nucleotide sequence of pKMK1, a cryptic plasmid isolated from Mycoplasma mycoides subsp. mycoides. It is 1875 bp in length and contains two open reading frames (ORFs) that share homology with ORFs from members of a large family of gram-positive bacterial plasmids which replicate via a single-stranded DNA intermediate. Putative origins of replication and candidate cloning sites have been identified.

Amino Acid Sequence↗
PubMed1991

Plasmid transformation of Mycoplasma mycoides subspecies mycoides is promoted by high concentrations of polyethylene glycol.

The recent isolation and characterization of two plasmids from Mycoplasma mycoides subspecies mycoides has opened up new possibilities for studying mycoplasmal genetics. In order to facilitate the development of a genetic system in M. mycoides subsp. mycoides, parameters of polyethylene glycol (PEG)-mediated transformation were examined, as existing protocols prove very inefficient in this organism. The effects of PEG concentration, DNA concentration, presence of Ca2+ ions, and choice of buffers on the transformation of the Tn916-containing plasmid pAM120 into M. mycoides subsp. mycoides were examined. The stability of Tn916 in the M. mycoides subsp. mycoides chromosome was also evaluated. The optimal PEG concentration (53-62% (w/v)) in the transformation mixture was substantially higher than the PEG concentration reported to be optimal for transformation of other mycoplasmas (36% (w/v)). The PEG concentrations used here were also higher than the concentration used to promote transformation or fusion of gram-positive bacterial protoplasts. A necessity for the presence of Ca2+ ions for optimal transformation was shown, as was the possible involvement of cell culture growth stage. Our results demonstrate the need for expanding current transformation techniques for mycoplasmas. Studies also indicate that once Tn916 inserts into the M. mycoides subsp. mycoides chromosome, it can transpose to other sites at a relatively high frequency.

Blotting, Southern↗
PubMed1988

An investigation of the external physician search process.

As a basis for better understanding the health care consumer and identifying ways to segment the health care market, the authors report the results of an empirical investigation of the external search process patients undertake to find a physician. The impact of a patient's perceived illness severity on this process also is investigated. Implications of the findings are discussed.

Adult↗
PubMed1978

Preventive and therapeutic benefits in relation to cost: performance over 10 years of Mothercraft Centers in Haiti.

Evaluation of the effectiveness of Mothercraft Centers operating in Haiti over the period 1964 to 1975 as a public health measure in private and public health services is reported. For the most part, the data are derived from centers operating routinely in the health services rather than from pilot demonstrations. Their impact in both therapy and prevention of severe malnutrition in preschool children is considered, and it is found that numerically the greater benefit is in prevention. At a total annual cost of $4034 a typical center accomplishes successful therapy and prevents relapse in 105 children. In addition, it provides protection against severe malnutrition to their 306 younger siblings. It is thus estimated that, considering only these 411 children demonstrably benefited from a year's operation, the cost of providing essentially lifetime protection from severe malnutrition is approximately $10 per child.

Body Weight↗