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Biomedical subjects

K Vogel

Publications and source records attributed to K Vogel.

At least 55 records · Page 3Linked to original sources
PubMed1989

[Determining the diagnosis--tumor of the kidney pelvis--using sonography].

The advantages and deficiencies of diagnostic imaging procedures especially of ultrasonography in the diagnostics of solid masses in the renal collecting system are presented. The intravenous urography is important as basic examination, but ultrasonography is valuable for additional information especially in the non-functioning kidney. A tumor could identified in the renal collecting system if its size is greater than 10 mm.

Aged↗
PubMed1988

Sequence and transcriptional start site of the Pseudomonas aeruginosa outer membrane porin protein F gene.

Porin F is one of the major proteins of the outer membrane of Pseudomonas aeruginosa. It forms water-filled pores of variable size. Porin F is a candidate for a vaccine against P. aeruginosa because it antigenically cross-reacts in all serotype strains of the International Antigenic Typing Scheme. We have isolated the gene for porin F from a lambda EMBL3 bacteriophage library by using oligodeoxynucleotide hybridization probes and have determined its nucleotide sequence. Different peptide sequences obtained from isolated porin F confirmed the deduced protein sequence. The mature protein consists of 326 amino acid residues and has a molecular weight of 35,250. The precursor contains an N-terminal signal peptide of 24 amino acid residues. S1 protection and primer extension experiments, together with Northern (RNA) blots, indicate that the mRNA coding for porin F is monocistronic with short untranslated regions of about 58 bases at the 5' end and about 47 bases at the 3' end. The sequences in the -10 and -35 regions upstream of the transcriptional start site are closely related to the Escherichia coli promoter consensus sequences, which explains why the porin F gene is expressed in E. coli under the control of its own promoter. The amino acid sequence of porin F is not homologous to the different E. coli porins OmpF, OmpC, LamB, and PhoE. On the other hand, a highly homologous region of 30 amino acids between the OmpA proteins of different enteric bacteria and porin F of P. aeruginosa was detected. The core region of the homology to E. coli OmpA had 11 of 12 amino acid residues in common.

Amino Acid Sequence↗
PubMed1988

[Congestive heart failure in a newborn infant with arteriovenous malformation of the vein of Galen: diagnosis using color-coded Doppler image echography].

Arteriovenous malformations of the vein of Galen are rare disorders that may appear in the newborn period with severe congestive heart failure mimicking many intrinsic cardiac defects. Using combined two-dimensional ultrasound and color-coded blood flow mapping arterio-venous aneurysm of the vein of Galen and congenital atrial septal defect could be diagnosed in an newborn with congestive heart failure. In addition to the presented clinical value of the new two-dimensional color Doppler echography physiological aspects of intracranial arteriovenous fistula in infancy discussed.

Cerebral Veins↗
PubMed1985

The core proteins of large and small interstitial proteoglycans from various connective tissues form distinct subgroups.

Large and small proteoglycans were separately isolated from a number of connective tissues and compared to determine the extent of structural similarity. This was studied by enzyme-linked immunosorbent assays and by the peptide patterns obtained when 125I-labelled proteoglycans were digested with trypsin. All the large proteoglycans, i.e. from tendon, sclera, cartilage and aorta, appear to contain the structure typical for the hyaluronic acid-binding region, both shown by enzyme-linked immunosorbent assay and by content of peptides unique for this region. These proteoglycans also share other structural features of the protein core, as indicated by immunological cross-reactivity and similar peptide patterns. The large proteoglycans from aorta in addition show the presence of unique structures both upon immunoassay and with regard to peptide pattern. Among the small proteoglycans two groups can be identified, although amino acid composition and protein core sizes are grossly similar. One group consists of the small proteoglycans from aorta and cartilage having similar peptide maps and showing immunological cross-reactivity in enzyme-linked immunosorbent assay. The other distinctly different group consists of the small proteoglycans from bone, cornea, sclera and tendon, which among them show identity in enzyme-linked immunosorbent assay and similar peptide patterns. Proteoglycans from the two groups, however, show partial immunological cross-reactivity.

Amino Acids↗
PubMed1982

Inactivation of a diol epoxide by dihydrodiol dehydrogenase but not by two epoxide hydrolases.

The mutagenicity of r-8,t-9-dihydroxy-t-10, 11-oxy-8,9,10,11-tetrahydrobenz[a]anthracene (BA-8,9-diol 10, 11-oxide) toward Salmonella typhimurium TA 100 is not decreased by the presence of large amounts of highly purified microsomal or cytosolic epoxide hydrolase. However, highly purified dihydrodiol dehydrogenase inactivates this diol epoxide, which is a major DNA-binding metabolite of benz[a]anthracene. The K-region epoxide, benz[a]anthracene 5,6-oxide (BA 5,6-oxide) is efficiently inactivated by microsomal epoxide hydrolase, is much less readily inactivated by cytosolic epoxide hydrolase, and is not inactivated by dihydrodiol dehydrogenase. This inactivation of a diol epoxide by dihydrodiol dehydrogenase points to a new significance of this enzyme and a new level of control for diol epoxides.

Alcohol Oxidoreductases↗
PubMed1980

Rat liver cytoplasmic dihydrodiol dehydrogenase. Purification to apparent homogeneity and properties.

A method is described for the purification of an enzyme, which catalyzes the conversion of benzene dihydrodiol to catechol, from rat liver cytoplasmic fraction to apparent homogeneity. The purification involved (NH4)2SO4 fractionation, DEAE-cellulose chromatography, interfacial salting-in and gel filtration through Sephadex G-100 superfine. The end product, which was purified over 500-fold with a yield of about 14% when compared to rat liver 100,000 X g supernatant, was judged to be homogeneous by several criteria, including sodium dodecyl sulfate-polyacrylamide gel electrophoresis, analytical ultracentrifugation, gel filtration, and immunoprecipitation. Physical studies suggested that the protein was a monomer with a molecular weight of 35,000 and one NADPH binding site per molecule. Amino acid analysis showed that the enzyme had a relatively high content of acidic and neutral amino acids in agreement with its isoelectric point which was at pH 6.2 Apparent Km values for benzene dihydrodiol and NADP+ were found to be 2.2 mM and 7.7 microM, respectively. The apparent Vmax value for the conversion of benzene dihydrodiol to catechol using NADP+ as cofactor was calculated to be 6.67 mumol/mg of enzyme/min. Substrate specificity studies showed that, in addition to benzene dihydrodiol, the dehydrogenase could oxidize acenaphthenol and the 3 alpha-hydroxy group of steroids. No activity was observable with a large number of other hydroxylated steroids possessing hydroxy groups at positions 3 beta, 11 beta, 17 alpha, 17 beta, 20 alpha, 20 beta, 21, and 22 of the steroid skeleton. Furthermore, only steroids which contained a 3-keto group and no double bond at the delta 4 position were reduced. This, and the fact that a range of nonsteroidal vicinal diols did not serve as substrates, indicates a relatively narrow substrate specificity. When benzene dihydrodiol was used as substrate, NADP+ was the preferred coenzyme but NAD+ was also accepted, whereas with the hydroxylated steroids the difference between the specific activities with NAD+ and NADP+ or with NADH and NADPH was less striking. The role of the enzyme in the metabolism of carcinogenic polycyclic hydrocarbons is discussed.

Amino Acids↗
PubMed1980

[Studies on the reliability of hard diagnosis of Marek's disease and lymphoid leukosis of chickens on the basis of pathologic-anatomical findings].

The reliability of criteria for diagnosis and differential diagnosis of Marek's disease and lymphoid leucosis of fowl, as part of herd diagnosis and on the basis of pathologico-anatomic findings as well as of pathomorphological and statistical tests has been checked for treatment in this paper. Tumorous lesions were macroscopically and histologically recorded from 1,261 hens of various industrialised poultry units. Marek's disease was established in 316 cases, lymphoid leucosis in 828 cases, mixed manifestations in 33 cases, and other neoplasias in 84 cases. These animals, together with 591 hens in which Marek's disease had been experimentally induced, were evaluated by pathologico-anatomic criteria of differential diagnosis. The conclusion was drawn that differential diagnosis of Marek's disease and lymphoid leucosis on the basis of pathologico-anatomic findings, without histological testing, was sufficiently reliable, when undertaken as part of herd diagnosis monitoring. The expected small number of false diagnoses will be unimportant to herd diagnosis. Both diagnosis and differential diagnosis will be conducted by the following criteria: age of animal, alterations caused by Marek's disease to nerves, eyes, and skin, tumour growth in bursa fabricii caused by lymphoid leucosis, atrophy of bursa fabricii caused by Marek's disease, as well as differences between both diseases regarding tumour development in other organs, with due consideration to be given to the number of affected organs, as a whole, and to pathologico-anatomic organic changes caused by tumour growth.

Animals↗
PubMed1980

[Staphylococcus aureus infection in chickens in industrialized poultry units. 1. Manifestations of Staphylococcus aureus infection in chickens].

Staphylococcus aureus infection has become an increasingly grave problem in industrialised poultry farming. This first communication in a series of publications on highly involved studies and experiments describes manifestations of the most common staphylococcal infections in fowl, including synovitis with arthritis, osteomyelitis, dermatitis, endocarditis, septicaemia, wound infection, and ophthalmitis. Reference in greater detail is made to manifestations with more important economic consequences, such as synovitis with arthritis, osteomyelitis, and dermatitis. Their frequency, dependence on age, clinical patterns, pathomorphology, pathogenesis are expounded together with peculiarities of differential diagnosis, with reference being made to findings obtained by the authors from their own studies on three industrialised poultry units.

Animals↗
PubMed1979

Liver-colonizing melanoma cells selected from B-16 melanoma.

Liver-colonizing tumor cells were selected from B-16 melanoma by a stepwise procedure: (1) injection of the original cells through the portal circulation: (2) removal of tumor cells from the hepatic tumor nodules for culture in vitro; (3) reinjection of such cells through the portal circulation and repetition of steps 2 and 3 eight times. The predilection of these cells to form tumors in the liver was clearly demonstrated following injection of the cells intra-arterially. The accuracy of the injection technique was controlled by monitoring amonts of injected radioactively labelled Sephadex microspheres. This liver-colonizing preference was not exclusive, but relative.

Animals↗
PubMed1979

[Epizootiology of Salmonella typhimurium infection in chickens].

The incidence of S. typhimurium infections among fowl increased in thr region of Potsdam in general, and on various big farms in particular, 1976 and over the first half of 1977. The outbreaks included subclinical infections and clinically manifest diseases which caused remarkable loss of broilers from the affected stocks (up to 15.92 per cent). Parent stocks contaminated with S. typhimurium were to be the sources of infection in all cases. A total of 1,220 Salmonella strains were isolated from fowl and its environment, with 1,151 of them being S. typhimurium (2.98 per cent of all samples tested). The following amounts of S. typhimurium strains were isolated from different types of samples which had been collected from infected broiler stocks: 8.10 per cent from dead broilers, 5.86 per cent from dead broiler parents, 2.11 per cent from pulp linings of transport cages for day-old chicks, 1.23 per cent from litter, 1.0 per cent from hatching material (eggs or dead and jammed embryos, and 0.12 per cent from swabs used in hygiene supervision). No Salmonellae were isolated from feedstuff. The transmission of S. typhimurium, therefore, is though to have taken the route via the hatching egg and via congenitally infected chicks traded between breeders and propagation farms. The control and prophylaxis of S. typhimurium infections, therefore, should be based primarily on action in the centralised breeding stocks. Specific steps of such action are proposed. Fifty-three strains were biochemically and lysotypically analysed, with the following types being determined: ut/Ph 30 BT b, ut/Ph 30 BT c, n.c. 1/72/n.c. BT b, 2 n.c. BT a, and 1A/6 BT a. The first two types covered 84.9 per cent of all strains isolated from the fowl. All lysotype ut/Ph 30 strains isolated from fowl fell under the copenhagen variant which had rarely been isolated from man in the past. These results are likely to support the demand for a joint control programme for enteritis Salmonellae, with particular emphasis on S. typhimurium, for implementation in human and veterinary medicine.

Animal Feed↗