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Biomedical subjects

K Ukai

Publications and source records attributed to K Ukai.

At least 109 records · Page 6Linked to original sources
PubMed1988

Morphological diversity of DRC-1 positive cells: human follicular dendritic cells and their relatives.

Follicular dendritic cells (FDC) and their relatives of the human tonsils and lymph nodes were immunoelectron microscopically analyzed with a monoclonal antibody DRC-1 (DAKO) in a combination with an enzymatic isolation technique. DRC-1+ cells were largely grouped into three type A (large mono- to multinucleated, sea anemone-fashioned, bearing labyrinth structures, in germinal center), type B (oligodendritic or stellate, medium-sized, in the corona and the primary follicle) and type C (individually located, medium-sized. lymphoid cell-fashioned, outside follicles). Type C were found in hyperplastic lymphoid tissue as well as in some granulation tissue. The DRC-1+ mononuclear cells were curiously encountered in the peripheral blood of some autoimmune disease patients in contrast with no identification in healthy persons. Furthermore some DRC-1+ cells obtained from autoimmune patient were confirmed to proliferate in culture.

Antibodies, Monoclonal↗
PubMed1988

A new monoclonal antibody, UFT-4, reacting with rat Kupffer cells. Immunohistochemical and immunoelectron microscopical analysis with reestimation of the reticuloendothelial system.

A new monoclonal antibody, designated UFT-4, reacting with rat Kupffer cells, was produced using sinusoidal liver-cell fraction as an immunogen. Immunohistochemically, UFT-4 reacted with Kupffer cells, interdigitating cells (IDCs), sinus endothelial cells of the spleen, a proportion of reticulum cells, smooth muscle fibers, choroid plexus epithelia and some macrophages. Immunoelectron microscopy disclosed two types of intracytoplasmic positivity: a striated type and a diffuse type. Both appeared to depend on variations in the quantity and distribution of the same antigen. On the other hand, UFT-4 gave a negative result for blood monocytes and most macrophages in lymphatic sinuses, lymphoid follicles, splenic red pulp and loose connective tissue. Cells positive for UFT-4 were mostly considered to belong to a narrow spectrum of the classical reticuloendothelial system, explaining the close relationship existing between some endothelia and some macrophages or reticulum cells. SDS-PAGE analysis of immunoprecipitates showed that the antigen reacting with UFT-4 was of molecule of 36-42 kDa under reducing conditions in the presence of 2-mercaptoethanol. The present results suggest that UFT-4 will be very useful for the study of Kupffer cells and for reconsidering their origin and destination.

Animals↗
PubMed1988

Effects of some psychotropic drugs on the b-wave of the electroretinogram in isolated rabbit retina.

The role of dopaminergic and cholinergic functions in the genesis of an electroretinogram is unclear. The present study was carried out to elucidate the direct actions of some psychotropic drugs in isolated rabbit retinas. Methamphetamine and apomorphine decreased dose-dependently the b-wave amplitude at a dose of 10(-7)-10(-5) g/ml. On the other hand, chlorpromazine and haloperidol, as well as atropine and amitriptyline, increased dose-dependently the b-wave amplitude at the same dose range. These data support the idea that dopaminergic and cholinergic systems play an important role in the genesis of the ERG.

Animals↗
PubMed1987

Indication of aorto-coronary bypass graft surgery for infarction area of myocardium considered from exercise thallium-201 myocardial imagings.

To assess accurately the indication and effects of aorto-coronary bypass graft surgery (ACBG) to the myocardium infarction area, 35 patients with myocardial infarction were analyzed by using exercise thallium-201 myocardial imagings. The patients were classified into group I (18 cases) and group II (17 cases) before ACBG. Group I consisted of patients with regional hypoperfusion in the initial image who showed redistribution in the delayed image to the infarction area of myocardium. Group II consisted of those with regional hypoperfusion in the initial image without redistribution in the delayed image. Group I showed a significant improvement in myocardial perfusion (p less than 0.01), pressure rate product (p less than 0.01), global left ventricular ejection fraction (p less than 0.01), segmental wall motion abnormality (p less than 0.01), and exercise tolerance when compared with those of group II before and after ACBG. The above study showed that the pre- operative redistribution of the infarction area in the delayed image suggests myocardial viability and ACBG will be effective.

Coronary Angiography↗
PubMed1987

[Thallium-201 scintigraphy of the myocardium during exertion in the indication for aortocoronary bypass of an area in the myocardium, the site of an infarct].

The purpose of this study is to precisely evaluate the indications and the results of direct revascularization surgery in a myocardial area previously infarcted. 35 cases were analyzed using thallium scintigraphy during stress. Before surgery, the patients were divided in two groups. Group I (18 cases) includes patients who presented initially a regional hypoperfusion and a reperfusion at the site of the infarction, on late scintigrams; group II (17 cases) also presented a regional hypoperfusion initially, but showed no reperfusion on late scintigrams. Concerning the results during stress, the thallium 201 scintigrams during exercise show alterations of the myocardial perfusion in the infarcted area, before and after surgery; in group I, a significant improvement (p less than 0.01) is noted in the entire group, i.e. 16 patients (88.9%), except in 2 cases where the graft was occluded, and group II shows an improvement in only 4 cases (NS) after surgery. Concerning the modifications of the product pressure X cardiac rhythm, there is a significant improvement (p less than 0.01) in group I (14 out of 18 patients (77.8%)); 2 out of 4 patients who were aggravated, presented occluded grafts after surgery. Group II shows an improvement in 10 out of 17 patients (58.8%) after surgery (NS). The left ventriculography demonstrates alterations in the global ejection fraction. Group I shows a significant improvement in 15 out of 18 patients (88.3%) (p 0.01); in group II there is improvement in 11 out of 17 patients (64.7%) (NS) after surgery.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗