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Biomedical subjects

K Ueda

Publications and source records attributed to K Ueda.

At least 1,567 records · Page 87Linked to original sources
PubMed1980

Poly(ADP-ribose) synthesis, a marker of granulocyte differentiation.

By using an indirect immunofluorescence technique, the distribution of poly(ADP-ribose) synthesis in human blood cells was investigated. The antibody used was reactive with poly(ADP-ribose) larger than trimers. The specific immunofluorescence of poly(ADP-ribose) synthesized in situ from NAD+ was observed in nuclei of lymphocytes and monocytes in normal peripheral blood. No immunofluorescence, however, was detected in granulocytes and erythrocytes. In agreement with this finding, no incorporation of radioactivity from [adenine-14C]NAD+ into acid-insoluble material was detectable in nuclei isolated from granulocytes. In normal bone marrow, the immunofluorescence of poly(ADP-ribose) was not observed in myelocytes or in their descendants but was observed in nuclei of lymphocytes and erythroblasts. Myelocytes and mature granulocytes in peripheral blood as well as in bone marrow of patients with chronic myelocytic leukemia were totally negative in the polymer-specific immunofluorescence. In marked contrast, prominent fluorescence was observed in nuclei of myeloblasts that appeared in peripheral blood as well as in bone marrow of patients with acute myeloblastic leukemia. Myeloblasts appearing in peripheral blood of patients in blastic crisis of chronic myelocytic leukemia also showed the nuclear immunofluorescence. These results suggest that the capacity for synthesizing poly(ADP-ribose) serves as a marker of differentiation of granulocytes, and its immunohistochemical analysis may be useful for differential diagnosis of leukemias, especially in blastic crisis.

Adenosine Diphosphate Ribose↗
PubMed1980

Susceptibility of germ-free mice to infectious megaenteron.

Germ-free (GF)-ICR mice were shown to be less susceptible to oral inoculation with a pathogenic strain of Escherichia coli (E. coli 0115a, c: K(B) than GF-CF No.1 mice. In GF-CF No. 1 mice a large number of organisms were recovered from the intestinal wall from the cecum to the rectum 3 to 7 days after inoculation. Unlike those in GF-CF No. 1 mice, lesions in GF-ICR mice were localized in a part of the cecum and organisms were recovered only from the cecal wall and rarely from organs other than those of the alimentary tract. In both strains of mice, however, organisms were recovered in large number from the intestinal contents. Histopathology and immunofluorescence revealed organisms closely attached to the surface of the cecum, colon and rectal epithelia in GF-CF No. 1 mice but only in a part of the cecal epithelium in GF=ICR mice. After being in contact with conventional CF No. 1 mice for 21 days and then inoculated orally with the pathogenic E. coli, ex GF-CF No. 1 mice died within 14 days with severe intestinal lesions, but ex-GF-ICR mice survived without lesions.

Animals↗
PubMed1980

HLA-linked genetic control in natural rubella infection.

HLA antigen frequencies were investigated in 115 patients with congenital rubella syndrome and 93 of their mothers in the Ryukyu Islands, Japan. A high frequency of HLA-B15 and a low frequency of HLA-Bw22 were found in the mothers. HLA-B15 was associated with high antibody responsiveness to rubella vaccine in our previous study, whereas HLA-Bw22 was one of the HLA antigens which showed low antibody responsiveness. There was no association between HLA antigens and viral replication in rubella vaccine recipients in the present study. These results indicate that and HLA-linked genetic background in the mothers is related to susceptibility to congenital rubella syndrome, and that the mechanism of the relation may be mediated via immune response genes.

Adolescent↗
PubMed1980

Immunohistochemical demonstration of poly(adenosine diphosphate-ribose) in nuclei of various rat tissues.

Natural distribution of poly(adenosine diphosphate-ribose), a novel macromolecule in eukaryotes, was investigated using an indirect immunofluorescence technique. The antibody, produced in a rabbit toward poly(ADP-ribose), was most reactive with polymers having the chain length of about 25 ADP-ribose units and weakly reactive with short oligomers; it was totally inert with monomers. Immunostaining with this antibody revealed the existence of the polymer in various rat tissues. The immunostaining seems to be specific for poly(ADP-ribose), as judged by its disappearance by preabsorption of the antiserum with purified poly(ADP-ribose) or pretreatment of tissue sections with poly(ADP-ribose)-degrading enzymes. Intensification of the fluorescence by preincubation with nicotinamide adenine dinucleotide (NAD), a substrate for poly(ADP-ribose) synthesis, also supported this view. The immunofluorescence of poly(ADP-ribose) was found exclusively in the nucleus of almost all tissues tested, including liver (adult, newborn, regenerating, and hepatoma), brain, heart, intestine, pancreas, kidney, spleen, testis, thyroid gland, and skeletal muscle. Exceptions were blood cells; little fluorescence was detectable in nuclei of peripheral leukocytes. Only after preincubation with NAD, did lymphocytes and monocytes exhibit fluorescence, however, granulocytes never did exhibit fluorescence. The cells appeared to represent the first instance where poly(ADP-ribose) synthesis activity among eukaryotic cells was missing.

Animals↗
PubMed1980

Sinoatrial responses to premature atrial stimulation: clinical observations and experimental study.

Two clinically-observed patterns in the response curve relating coupling intervals of premature atrial stimulation to return cycles were experimentally reproduced and studied by microelectrode techniques in the sinoatrial preparations of rabbit hearts. The progressive prolongation pattern of the return cycles with shortening of coupling intervals was associated with progressive depressions both of atriosinus conduction and sinus node automaticity. The progressive shortening pattern of the return cycles was associated with progressive shortening in the duration of sinus node action potential in two-thirds of the preparations and with pacemaker shift in the remaining one-third of them. Possible "sinus node reentry" was demonstrated. The mechanisms and clinical implications of these phenomena were discussed.

Action Potentials↗
PubMed1980

The comparison of hemodynamic effects of concomitant use of intra-aortic balloon pumping and veno-arterial bypass with atrio-arterial bypass.

Atrio-Arterial Bypass (AAB) and concomitant use of Intra-Aortic Balloon Pumping (IABP) and Veno-Arterial Bypass (VAB) were performed, using 7 dogs with induced cardiogenic shock. AAB was established by draining blood from the left atrium and subsequently returning this blood to the carotid artery. VAB was also accomplished by draining blood from the right atrium and then returning this blood to the femoral artery. And bypass flow rates were set at approximately one third of the baseline cardiac output. Evaluation of hemodynamic effects of concomitant use of IABP and VAB was carried out, compared with AAB. The following findings were obtained. 1) In concomitant use, the left ventricular afterload was decreased to lower level (mSPr and TTI) and the total perfusion flow was increased to higher level than in AAB. 2) In AAB the left ventricular preload (mLAP) decreased to lower level than in concomitant use. 3) In concomitant use and AAB, the left ventricular stroke work decreased to the same level.

Animals↗
PubMed1980

Influence of vasodilator therapy on prognosis of acute myocardial infarction in the aged patients. A comparative study between vasodilator-treated group and group treated without vasodilator.

Influence of vasodilator therapy on prognosis of acute myocardial infarction complicated by left heart failure (in Killip classes II and III) was studied in 22 aged patients (mean age; 75.7 years). The patients were retrospectively divided into 2 groups: Group I; 12 patients were treated with isosorbide dinitrate and/or chlorpromazine and Group II; 10 patients were treated with other conventional medical measures. Initial hemodynamic changes on admission were not significantly different between 2 groups. With these treatments mortality rate within 2 weeks of treatment was lower in Group I than in Group II (p less than 0.01), however, cumulative cardiac mortality rate within 18 months did not differ significantly. The present study demonstrated beneficial influence of vasodilator therapy on early prognosis in aged patients, but failed to show prolonged influence on later mortality.

Age Factors↗