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Biomedical subjects

K Ueda

Publications and source records attributed to K Ueda.

At least 1,063 records · Page 59Linked to original sources
PubMed1989

Effect of protein kinase C inhibitor (H-7) and calmodulin antagonist (W-7) on pertussis toxin-induced IL-1 production by human adherent monocytes. Comparison with lipopolysaccharide as a stimulator of IL-1 production.

Human adherent monocytes stimulated with 1 microgram/ml pertussis toxin (PT) produced interleukin-1 (IL-1), as measured by thymocyte co-stimulation assay and enzyme-linked immunosorbent assay (ELISA), specific for IL-1 alpha and IL-1 beta. To clarify the role of protein kinase C (PKC) and calmodulin in IL-1 production, we investigated the effects of a PKC inhibitor, H-7, and a calmodulin antagonist, W-7 on PT- and lipopolysaccharide (LPS)-induced IL-1 production by monocytes. Addition of 10 microM and 20 microM H-7 to the culture medium markedly suppressed both PT- and LPS-induced IL-1 production. PT-induced IL-1 production was significantly suppressed by 5 microM and 10 microM W-7. However, LPS-induced IL-1 production was not suppressed by W-7 at the concentrations tested. When monocytes were labelled with Quin 2/AM, IL-1 production by monocytes stimulated with PT and LPS was markedly suppressed. These results indicate that different pathways are involved in the IL-1 production by PT and LPS; both calmodulin- and PKC-dependent processes are necessary for the IL-1 production induced by PT, whereas LPS-induced IL-1 production is dependent on the PKC. Inhibition of IL-1 production by interfering with intracellular Ca2+ trafficking in Quin 2/AM-loaded monocytes may be associated with the inhibition of PKC and calmodulin activity.

1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine↗
PubMed1989

[Detection of antineutrophil antibodies in patients with neutropenia in infancy].

Antineutrophil antibodies in the sera against peripheral blood neutrophils were examined in 8 infants (2 to 12 months of age) with neutropenia (absolute neutrophil count 0 to 480/microliter). The antibodies were detected by granulocyte indirect immunofluorescence test (GIIFT) and microleukocyte agglutination test (M-LAT). Three of the 6 serum samples with chronic benign-type neutropenia reacted in both GIIFT and M-LAT. In 2 of these 3 samples, the antibody was proved to be specific to neutrophil antigen NA1 by the absorption experiment. Neither the GIIFT nor the M-LAT showed the antibody in two patients with Kostmann-type neutropenia. These results suggest that the examination of antibodies against neutrophils is essential to the evaluation of the patients with chronic benign-type neutropenia in infancy.

Agranulocytosis↗
PubMed1989

[Expression of pre-S1 antigen and pre-S1 antibody in sera obtained from HBV infected patients].

We investigated the expression of Pre-S1 antigen and Pre-S1 antibody using a synthetic peptide and the monoclonal antibody against it. Four patients with acute hepatitis B and 87 chronic HBsAg carriers were included in this study. There was a significant correlation between Pre-S1 antigen titers and HBsAg titers. Pre-S1 antigen showed higher titers in patients with active viral replication, positive for HBeAg, HBV-DNA or HBV-related DNA polymerase. The ratio of Pre-S1 antigen titers to HBsAg titers, however, had no significant relationship with those replicative markers. It was suggested that Pre-S1 antigen was expressed with an intimate relation to the expression of HBsAg and was not so useful as a new replicative marker. Pre-S1Ag titers/HBsAg titers tended to be high in patients with chronic active hepatitis and high serum GPT levels. This may be due to the release of overproduced intracellular Pre-S1 antigen. Pre-S1 antibody was detected only in few cases of chronic HBsAg carriers. This result shows that the immune tolerance to Pre-S1 region may play a role in the persistence of HBV infection.

Carrier State↗
PubMed1989

[Two-dimensional echocardiographic findings of cor triatriatum: differential diagnosis from total anomalous pulmonary venous connection to the coronary sinus].

Two-dimensional echocardiograms were reviewed in six patients with cor triatriatum (CTA) and six patients with total anomalous pulmonary venous connection to the coronary sinus (TAP) to characterize the echocardiographic features of distinguishing both diseases. Patients' ages ranged from nine days to 25 months in CTA, and from one to 11 months in TAP. The echocardiographic findings of CTA included three findings which were previously reported; the "double arch appearance" of the intra-atrial membrane, a "recess" which developed between the posterior wall of the aorta and the membrane, and the small size of the "recess", and two additional peculiar findings: the direction and echo density of the intra-atrial membrane. The "double arch appearance" of the intra-atrial membrane could not be detected in any of the CAT nor in TAP cases, except in one TAP patient who had a drainage vein obstruction. The "recess" was found in all six TAP patients, but it was recognized only in three of six patients with CTA. Among six patients with TAP, the size of a "recess" was small in two and large in one patient. Among three patients with CAT who had a "recess", the size was small in two and large in one patient: Thus, the size had little to do with differentiating CTA and TAP. The intra-atrial membrane ran parallel with the mitral valve ring in all patients with CTA except one in whom the membrane could not be identified because of the presence of endocardial cushion defect and a round membrane. On the contrary, the membrane ran parallel with the posterior wall of the common pulmonary venous chamber in all patients with TAP. The echo of the membrane was of low density in all patients with CAT and in three of six patients with TAP. The "double arch appearance" of intra-atrial membrane, the presence of a "recess", and the size of the "recess" were not characteristic echocardiographic features which could distinguish CAT from TAP. However, the direction of the membrane was useful as a means of distinguishing two anomalies. The intra-atrial membrane runs parallel with the posterior wall of the common pulmonary venous chamber in TAP, and with the mitral valve in CTA. The echo density of the membrane could not clearly differentiate CAT from TAP, but it was of low density in all patients with CTA.

Child, Preschool↗
PubMed1988

Three types of amyloid protein precursor mRNA in human brain: their differential expression in Alzheimer's disease.

Three types of amyloid protein precursor (APP) mRNA, produced by alternative splicing, were detected by Northern blotting in human brains, both control and Alzheimer's disease. These mRNAs encode APP695 consisting of 695 amino acids, APP751 harboring a 56 amino acid insert homologous to a Kunitz-type trypsin inhibitor inside APP695, and APP770 containing an additional 19 amino acid insert. Another possible APP mRNA which encodes "APP714" containing a 19 amino acid insert was not found in brain samples tested. Quantitative analysis revealed that, although the relative expression levels of the three mRNAs were variable among individuals, there was no remarkable change in expression of APP695 and APP751 mRNAs in Alzheimer's disease compared with control, but that APP770 mRNA level was elevated significantly in Alzheimer's disease.

Alzheimer Disease↗
PubMed1988

Tight binding of glucocorticoid-receptor complexes to histone-agarose.

"Activated" glucocorticoid-receptor complexes purified about 3,000-fold from rat liver were found to bind to histone-agarose. Because of their tight binding, they could not be eluted from the column by high salt solution (3 M KCl) or low salt plus polyol buffer (50% ethylene glycol), but their binding could be disrupted by pyridoxal 5'-phosphate; more than 70% recovery of the "activated" receptor complexes was achieved with buffer containing 20 mM pyridoxal 5'-phosphate. This interaction of "activated" glucocorticoid-receptor complexes of rat liver with histone-agarose suggests a role of histones in the mechanism of action of steroid hormone.

Animals↗
PubMed1988

Vincristine-induced fever in children with leukemia and lymphoma.

Thirty-one children with leukemia or lymphoma treated with multi-drug chemotherapy for more than 2 years were reviewed to identify and characterize the occurrence of febrile episodes related to vincristine (VCR) injection. In nine of the 31 children, more than two febrile episodes apparently attributable to VCR during maintenance therapy were identified. No such episodes were found to have occurred during induction therapy. The median age at diagnosis of these nine children was 3.2 +/- 1.6 years, significantly lower than the 6.8 +/- 4 years of the other 22 children (P less than 0.01). No significant difference was observed between these two groups in laboratory data obtained before the VCR injections, and the drugs used in combination with VCR were apparently unrelated to the incidence of febrile episodes. All of these febrile episodes began within 24 hours after VCR injection. Peak levels of 38 degrees C to 39 degrees C occurred 6 to 24 hours after onset. The episodes also were accompanied by mild, general fatigue and a loss of appetite. They ranged in duration from half of a day to 4 days, but those persisting more than 3 days occurred in three of the five children whose regimens did not include corticosteroid. The results thus suggest that fever is an immediate reaction to the toxicity of VCR in young children, and that the duration of fever can be shortened by combining VCR with corticosteroid.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1988

DNA damage induced by ascorbate in the presence of Cu2+.

DNA damage induced by ascorbate in the presence of Cu2+ was investigated by use of bacteriophage phi X174 double-stranded supercoiled DNA and linear restriction fragments as substrates. Single-strand cleavage was induced when supercoiled DNA was incubated with 5 microM-10 mM ascorbate and 50 microM Cu2+ at 37 degrees C for 10 min. The induced DNA damage was analyzed by sequencing of fragments singly labeled at their 5'- or 3'-end. DNA was cleaved directly and almost uniformly at every nucleotide by ascorbate and Cu2+. Piperidine treatment after the reaction showed that ascorbate and Cu2+ induced another kind of DNA damage different from the direct cleavage. The damage proceeded to DNA cleavage by piperidine treatment and was sequence-specific rather than random. These results indicate that ascorbate induces two classes of DNA damage in the presence of Cu2+, one being direct strand cleavage, probably via damage to the DNA backbone, and the other being a base modification labile to alkali treatment. These two classes of DNA damage were inhibited by potassium iodide, catalase and metal chelaters, suggesting the involvement of radicals generated from ascorbate hydroperoxide.

Ascorbic Acid↗
PubMed1988

Morphology of physiologically identified mitral cells in the carp olfactory bulb: a light microscopic study after intracellular staining with horseradish peroxidase.

Physiologically identified mitral cells in the carp olfactory bulb were stained by intracellular injection of horseradish peroxidase in order to study the morphology in detail. The somata were fusiform, elongated, oval, triangular, or irregular. The mean diameters of the somata were 30 microns X 14 microns. Two to five thick dendrites arose from the somata and frequently gave off branches to form glomerular tufts. The dendrites extended less than 400 microns; the dendritic field of single mitral cells in the medial or lateral part of the olfactory bulb was confined within the respective part of the bulb. The axons arose from either the somata or the dendrites and had a conical initial portion, usually with a smooth contour. Some cells had poorly developed intrabulbar axon collaterals. No difference between the mitral cells in the medial part of the olfactory bulb and those in the lateral part was found in the soma diameter, the dendritic diameter at the base, or the number of first-order dendrites. However, there was a difference in the site of the origin of the axon between them: most of the axons of the mitral cells in the medial part arose from the dendrites, while most of the axons of the mitral cells in the lateral part arose from the somata. The morphology of physiologically identified mitral cells is basically consistent with that reported in the Golgi studies of teleosts. The limited dendritic fields of mitral cells may underlie the previously reported functional separation of the olfactory bulb into medial and lateral parts. The results also indicate that the two parts of the teleost olfactory bulb are differentiated not only functionally but also morphologically.

Action Potentials↗
PubMed1988

Blood pressure in Japanese children during the first three years of life. The Hisayama Study.

Blood pressure (BP) measurements were obtained from 522 healthy young children aged from 3 months to 3 years in Hisayama, Japan. The measurements were performed using a Doppler ultrasound device applied to children who were awake and sitting quietly on their mothers' laps. This method of obtaining BP was successful in 80% to 90% of children aged 3, 6, and 36 months, and in 60% to 70% of children aged between 12 and 18 months. Mean systolic BP varied from 88 mm Hg at 3 months to 96 mm Hg at 3 years and showed a tendency to elevate with increasing age, with the increment being the greatest between ages 3 and 6 months. Mean diastolic BP was constant throughout the first three years of life.

Aging↗
PubMed1988

C7 deficiency and persistent haematuria.

A 14-year-old boy had persistent haematuria along with complete C7 deficiency. No significant changes in glomeruli and tubules were found in a renal biopsy specimen by light microscopy and immunofluorescence gave negative results for immune deposits. Electron microscopic examination demonstrated an attenuation of the glomerular capillary basement membrane without lamination and a diagnosis of thin basement membrane disease was made. It would be difficult to conclude that patients with C7 deficiency were predisposed to develop glomerulonephritis caused by immunologic aberrations. A family study failed to provide evidence of an association of C7 deficiency and thin basement membrane disease.

Adolescent↗
PubMed1988

Thrombocytopenia: a complication of Kawasaki disease.

Thrombocytopenia was observed in 10 (2.0%) of 486 children with Kawasaki disease. In nine of the ten, the minimal platelet count of 94,000 +/- 38,000 (SD)/mm3 was seen on day 6.8 +/- 2.2 (SD) of illness and the platelet counts were elevated to the normal level in 1-2 weeks. Thrombocytopenia in the nine appeared to be caused via coagulation-mediated platelet consumption, while the remaining child was diagnosed as having idiopathic thrombocytopenic purpura. One of the two who had severe coagulation-mediated thrombocytopenia of less than 50,000/mm3 developed coronary aneurysms persisting over 1 year.

Aspirin↗
PubMed1988

Neuronal pathways for the lingual reflex in the Japanese toad.

1. Anuran tongue is controlled by visual stimuli for releasing the prey-catching behavior ('snapping') and also by the intra-oral stimuli for eliciting the lingual reflex. To elucidate the neural mechanisms controlling tongue movements, we analyzed the neuronal pathways from the glossopharyngeal (IX) afferents to the hypoglossal (XII) tongue-muscle motoneurons. 2. Field potentials were recorded from the bulbar dorsal surface over the fasciculus solitarius (fsol) to the electrical stimulation of the ipsilateral IX nerve. They were composed of three successive negative waves: S1, S2 and N wave. The S1 and S2 waves followed successive stimuli applied at short intervals (10 ms or less), whereas the N wave was strongly suppressed at intervals shorter than 500 ms. Furthermore, the S1 wave had lower threshold than the S2 wave. 3. Orthodromic action potentials were intra-axonally recorded from IX afferent fibers in the fsol to the ipsilateral IX nerve stimuli. Two peaks found in the latency distribution histogram of these action potentials well coincided with the negative peaks of the S1 and the S2 waves of the simultaneously recorded field potentials. Therefore, the S1 and S2 waves should represent the compound action potentials of two groups of the IX afferent fibers with different conduction velocities. 4. Ipsilateral IX nerve stimuli elicited excitatory postsynaptic potentials (EPSPs) in the tongue-protractor motoneurons (PMNs) and the tongue-retractor motoneurons (RMNs). Inhibitory postsynaptic potentials were not observed. 5. The EPSPs recorded in PMNs had mean onset latencies of 6.4 ms measured from the negative peaks of the S1 wave. The EPSPs were facilitated when paired submaximal stimuli were applied at intervals shorter than 20 ms, but were suppressed at intervals longer than 30 ms. Furthermore, the EPSPs were spatially facilitated when peripherally split two bundles of the IX nerve were simultaneously stimulated. 6. On the other hand, the EPSPs recorded in RMNs had shorter onset latencies, averaging 2.5 ms. In 14 of 43 RMNs, early and late EPSP components could be reliably discriminated. The thresholds for the early EPSP components were as low as those for the S1 waves, whereas for the late EPSP components the thresholds were usually higher than those for the S2 waves.(ABSTRACT TRUNCATED AT 400 WORDS)

Action Potentials↗
PubMed1988

Alpha-interferon treatment for adult T cell leukemia: low levels of circulating alpha-interferon and it's clinical effectiveness.

We describe a patient with adult T cell Leukemia to whom alpha-interferon therapy was highly effective. Although a combination chemotherapy (ACVP) first introduced was effective in reducing total leukocyte counts, the percentage of leukemic cells relative to total leukocyte counts was decreased first after the institution of alpha-interferon therapy. The patient is now under complete remission for four years. It was noted in this patient that circulating alpha-interferon, measured by a sensitive radioimmunoassay, was consistently low as compared with the value found in the age-, sex-matched healthy control (p less than 0.001). Since adult T cell leukemia is pathogenetically related to the retrovirus infection, low levels of circulating alpha-interferon of the patient may be important from both pathogenetic and therapeutic standpoints. Alpha-interferon therapy may be an useful additive for the chemotherapy of adult T cell leukemia.

Adult↗