Search PubMed⌕ Search

Biomedical subjects

K Tsuji

Publications and source records attributed to K Tsuji.

At least 955 records · Page 53Linked to original sources
PubMedDate not supplied

Hyperthermic enhancement of tumour growth inhibition by accelerated carbon-ions in transplantable human esophageal cancer.

The study examined the effects of combination of hyperthermia (42 degrees C) and 290 MeV/u carbon-ion (C-) beams or 200 kVp X-rays on tumour regrowth delay of transplantable human esophageal cancer as an in vivo model for radiotherapy of cancer. The C-beams were more effective in the tumour growth inhibition than X-rays. The relative biological effectiveness (RBE) of C-beams against X-rays was 2.00. It was observed that the interactive hyperthermic (42 degrees C, for 30 min) enhancement of tumour regrowth delay by high-linear energy transfer (LET) C-beams was similar to that of combination of low-LET X-rays with hyperthermia. The thermal enhancement ratios (TER) were 6.10 and 5.57 for X-rays and C-beams, respectively. These results suggest that hyperthermic treatment is effective in radiotherapy not only by low-LET radiation but also by high-LET radiation such as C-beams. In conclusion, the depression of the tumour growth by the combined treatment of hyperthermia (42 degrees C) and the C-beams strongly suggests the available possible application of interdisciplinary cancer therapy for refractory tumours.

Aged↗
PubMedDate not supplied

Expression of G-CSF receptor on myeloid progenitors.

Although granulocyte colony-stimulating factor (G-CSF) has been reported to act on cells of neutrophilic lineage, the expression of receptors for G-CSF (G-CSFR) on human hematopoietic progenitor cells has been unclear. We then analyzed the expression of G-CSFR on human bone marrow and G-CSF mobilized peripheral blood CD34+ cells, and examined the proliferation and differentiation capabilities of sorted CD34+ G-CSFR+ and CD34+ G-CSFR- cells using methylcellulose clonal culture. These results indicate that the expression of G-CSFR on CD34+ cells is restricted to myeloid progenitors, suggesting that the specific activity of G-CSF on myelopoiesis depends on the exclusive expression of its receptor on myeloid progenitors, and that the mobilization of various hematopoietic stem/progenitor cells is not a direct effect of G-CSF in humans.

Bone Marrow Cells↗
PubMedDate not supplied

HLA antigens in atopic diseases.

The immunogenetical relationship among atopic diseases was investigated from the point of human major histocompatibility antigen. The relationship between IgE level and HLA antigen was also discussed. (1) Atopic dermatitis had no high frequency in HLA antigens. HLA-B12 in bronchial asthma and HLA-BW40 in allergic rhinitis were frequently found in the population study. (2) No HLA-BW15 was observed in these three diseases. The term called 'disease-resistant antigen' was proposed in this paper. (3) We considered that IgE level is not controled by HLA antigen.

Adolescent↗
PubMedDate not supplied

Generation of HLA-DP transgenic pigs for the study of xenotransplantation.

The shortage of human organs has prompted scientists to seek xenogeneic sources of donors. To date, DAF, MCP, and CD59 transgenic pigs have been generated to inhibit hyperacute rejection. However, besides hyperacute rejection, acute and chronic rejection must also be considered in the use of porcine organs for xenotransplantation. The role of HLA-II in transgenic xeno-organ transplantation remains to be elucidated. By microinjecting 1655 embryos, we have generated one stillborn HLA-DR and two live HLA-DP transgenic pigs: P113-7 (male, carrying one copy of exogene) and P113-8 (female, carrying 2-3 copies of exogenes). The gene status of the live transgenic pigs was confirmed by PCR, Southern blot, and PCR product sequencing analysis. The expression of transgenes in these transgenic pigs were confirmed by RT-PCR analysis and immunohistochemical staining of frozen sections of ear tissue.

Animals↗
PubMedDate not supplied

An effective pre-operative chemoimmunotherapy regimen against advanced gallbladder carcinoma: a case report.

We designed a chemoimmunotherapy regimen with a single dose of mitomycin C (MMC) followed by subsequent OK-432 injections, since the ability of peripheral blood mononuclear cells (PBM) to generate OK-432 activated killer cells had previously been shown to be augmented by MMC in cancer patients. We herein report on a case with far advanced gallbladder carcinoma who demonstrated a remarkable response to this chemoimmunotherapy, and thus underwent a curative resection. A 67 year-old Japanese woman was diagnosed as having gallbladder carcinoma with invasion to the liver and portal vein, as well as obvious lymph node metastasis. Since these findings suggested the tumor to be unresectable at that time, our chemoimmunotherapy regimen with MMC and OK-432 was administered. After four courses of therapy, a computed tomography (CT) scan revealed the disappearance of both tumor invasion to the surrounding organs and lymph node metastasis, which therefore prompted us to attempt a radical tumor resection. The histology of the resected specimen revealed that the majority of cancer cells had been killed by the pre-operative therapy and that only remnants of viable cancer cells were found in a part of the neck of the gallbladder and in 2 regional lymph nodes. This experience thus suggests the effectiveness of our chemoimmunotherapy regimen against gallbladder carcinoma.

Aged↗
PubMedDate not supplied

Leiomyoma of the parapharyngeal space.

A rare case of leiomyoma of the prestyloid parapharyngeal space is reported together with computed tomographic and magnetic resonance imaging findings. The tumor appeared as a well-circumscribed ovoid mass with some calcifications and lobulations. Because the attenuation, signal intensity, and shape of the mass were not specific and were similar to those of a pleomorhic adenoma, the most common tumor of the prestyloid parapharyngeal space, radiological differentiation of leiomyoma from pleomorphic adenoma of the prestyloid parapharyngeal space was difficult.

Adult↗
PubMedDate not supplied

New method for determining total dietary fiber by liquid chromatography.

The molecular weight limit of water-soluble dietary fiber (SDF) determined by the Prosky method was studied by liquid chromatography (LC). It was confirmed that only SDF with an average degree of polymerization of 12 or higher can be determined by the Prosky method. Total dietary fiber (TDF) was determined by 2 additional methods using LC. In the first method, the total quantity of insoluble dietary fiber (IDF) and high molecular weight SDF (HMSDF) was determined according to the modified Prosky method (MES-TRIS buffer-based). The quantitatively collected final filtrate was analyzed by LC for the quantity of low molecular weight SDF (LMSDF), and the 2 quantities were totaled to obtain TDF. TDF values thus determined for rice, polished or unpolished, soybean flour, and pressed barley were higher than those determined by the Prosky method by approximately 6, 3.5, and 3.5%, respectively. In the second method, direct determination by LC analysis was done on samples after enzymatic treatment according to the Prosky method. Results showed that the determination of LMSDF, in particular, was highly accurate and more effective. In both of these methods, the quantity of LMSDF was determined from its chromatographic peak area ratio to glucose as an internal standard, which was produced by hydrolysis.

Carbohydrates↗
PubMedDate not supplied

Human neutrophil functions in obstructive jaundice.

BACKGROUND/AIMS: The effect of obstructive jaundice on neutrophil function has not been extensively studied. Therefore, the present study aimed at evaluating the effect of obstructive jaundice on human neutrophils. METHODOLOGY: Twelve patients with obstructive jaundice due to common bile duct obstruction underwent endoscopic biliary drainage. Neutrophil functions (chemotaxis and superoxide anion generation) were evaluated before and 7 days after drainage. RESULTS: Neutrophil chemotaxis in response to FMLP (formyl-methionyl-leucyl-phenylalanine) or interleukin-8 was abnormally increased before drainage, and was normalized after drainage. Similarly, enhanced superoxide anion generation in response to FMLP or phorbol myristate acetate before drainage was alleviated after drainage. CONCLUSIONS: The results suggest neutrophil overactivity in patients with obstructive jaundice. The ameliorating effect of biliary drainage on neutrophil overactivity might play a role in the prevention of postoperative complications.

Adult↗
PubMedDate not supplied

Trace analysis of microcystins in environmental samples.

Microcystins, the cyclic heptapeptide toxins produced by cyanobacteria such as Microcystis sp., show potent hepatotoxicity and tumor-promoting activity through inhibition of protein phosphatases 1 and 2A. A toxic incident resulting in the death of 50 people in Brazil in 1996 was due to microcystins in water used for hemodialysis. Microcystins are now threatening human health and life, but many problems associated with microcystins remain unsolved. This report describes how to analyze trace amounts of microcystins in complicated matrixes such as liver tissue, lake water, and sediments.

Animals↗
PubMedDate not supplied

External radiotherapy for decompression of cholangiocellular carcinoma with obstructive jaundice: report of a case.

Percutaneous transhepatic catheter drainage (PTCD) is generally performed for the treatment of obstructive jaundice. However, in some cases it is difficult to perform insertion because of lack of dilatation of the intrahepatic bile duct. We report a patient who had been suffering from jaundice as a result of recurrent cholangiocarcinoma near the hilum and whose symptoms were relieved by external radiotherapy. We consider radiotherapy for cholangiocarcinoma effective palliative treatment for decompression of obstructive jaundice.

Bile Duct Neoplasms↗
PubMedDate not supplied

Automation and computerization of chromogenic LAL assay method for bacterial endotoxin using 96-well microtiter plate.

A high degree of automation was achieved for a chromogenic substrate Limulus amebocyte lysate (LAL) assay method for bacterial endotoxin using a Cetus Pro/Group Liquid Handler and 96-well microtiter plates. A Titertek Multiskan Reader was interfaced with an IBM PC using a LOTUS MEASURE software to capture optical density values of samples in a LOTUS 1-2-3 spreadsheet. A password protected, menu-driven macro programmed in LOTUS 1-2-3 automates the calculation, evaluation of assay parameters, documentation, and generation of a formatted three-page report suitable as a primary record. All assay operations, including testing 19 samples against a four-point standard curve in replicates of four each, the calculation of results, and generation of a report, are completed in less than 40 minutes. The relative standard deviation (RSD) of the assay is approximately 7%, which compares favorably with the robot automated system.

Automation↗
PubMedDate not supplied

Expert system computer program for troubleshooting the gel-clot Limulus amebocyte lysate (LAL) assay.

This article describes a knowledge-based or expert system computer program that can assist in troubleshooting the gel-clot Limulus Amebocyte Lysate (LAL) method for the assay of endotoxin in parenteral products and in-process materials. The program may also be used to train new personnel and/or to assist in the development of an LAL method for a new product or material. The program includes LAL assay information for 31 parenteral products and materials. The program uses rules-of-thumb and intuition to diagnose 155 possible LAL assay problems and logically reduce them to 37 recommended problem solving processes. The program also provides references to support its recommendation.

Endotoxins↗
PubMedDate not supplied

An attempt of hyperthermia with balloon catheter and immunochemotherapeutic agent.

The following studies were performed to develop a new technique of hyperthermia by vascular catheterization for the treatment of malignant neoplasm. A balloon catheter was inserted into the artery of a cancer-affected organ and inflation of the balloon temporarily occluded the artery, after which an anticancer drug solution warmed to 45 degrees C was infused. The purpose of this technique was to enable the solution to arrive at the affected area undiluted by blood. It would then remain in the organ and act on the lesion. When OK-432 was intravenously administered to induce pyrexia of 40 degrees C, followed by the above-mentioned arterial infusion, it was expected that the effects of hyperthermia and chemotherapy would be synergistic. In cases of liver cancer and urinary bladder cancer subjected to the arterial infusion of warmed anticancer drugs, however, the temperature in the hepatic vein increased by only 0.5 degree C while that in urethra near the tumor increased by only 1 degree C. In the future, further improvement needs to be made to obtain effective local heating with this technique.

Antineoplastic Combined Chemotherapy Protocols↗
PubMedDate not supplied

Gas chromatographic determination with electron capture detection of residual ethylene oxide in intraocular lenses.

A sensitive method is described to determine trace quantities of ethylene oxide (EO) in EO-sterilized intraocular lenses (IOLs). An IOL is dipped in ethanol containing 0.25 ppm propylene oxide (PO) in a 4 mL vial, 2 drops of freshly distilled hydrobromic acid is added through a septum, and the mixture is warmed at 50 degrees C for 24 h. It is then neutralized by vigorous shaking with sodium bicarbonate, dehydrated with anhydrous sodium sulfate, and filtered. The filtrate is injected into a gas chromatograph with electron-capture detection, and the peak height ratio of ethylene bromohydrin/propylene bromohydrin is measured. EO residue is calculated from the calibration curve obtained through a similar procedure with the standard EO/PO solutions. The limit of determination is 0.04 microgram/lens (ca 2.0 ppm). When EO residue levels were determined for IOLs sampled at 3 different aeration periods after sterilization, we found that 9 days of aeration was necessary to meet the U.S. Food and Drug Administration proposed limit for EO residue in IOLs.

Chromatography, Gas↗
PubMedDate not supplied

Familial Sjögren's syndrome in the Japanese: immunogenetic and serological studies.

Serological abnormalities and HLA haplotypes were studied in a Japanese family of two patients with Sjögren's syndrome (SS) associated with other autoimmune diseases. In contrast to the reports in the U.S.A. and Europe, we found a significant excess of HLA-DRw53 antigen in the family members. Two family members, mother and niece of the probands, had suffered from other connective tissue diseases. Although none of the siblings of probands had manifestations of connective tissue diseases, two siblings had several autoantibodies. No consistent segregation, however, was found between the HLA haplotypes and the serological abnormalities in the relatives. Therefore, HLA alone can not explain the familial clustering of autoimmune diseases including SS and of autoantibodies.

Adult↗