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Biomedical subjects

K Tsuji

Publications and source records attributed to K Tsuji.

At least 919 records · Page 51Linked to original sources
PubMed1966

Liquid nitrogen preservation of Saccharomyces carlsbergensis and its use in a rapid biological assay of pantothenic acid.

A rapid direct turbidimetric determination of pantothenic acid with the use of Saccharomyces carlsbergensis was developed. With a 5.5-hr incubation, the bioassay demonstrated good dose responses within a range up to 0.04 mug/ml of calcium pantothenate. The multivitamin product assay values obtained by the rapid assay method compare well with the values obtained by the standard USP method. beta-Alanine interference was reduced to an insignificant level by the incorporation of asparagine in the assay medium. The Upjohn complex products which require Mylase-P enzyme digestion treatments were also satisfactorily assayed.

Alanine↗
PubMedDate not supplied

Correlation between microstructure of Ag-Pd-Au-Cu alloy and changes in shape on aging.

An investigation has been carried out to study changes in shape on aging in plastic deformed Ag-Pd-Au-Cu alloys. The changes in shape were measured in order to establish a relationship to the aging heat treatments. Their microstructure was observed in order to clarify this phenomenon at the same time. They were also investigated quantitatively using a feature analysis system. The changes in shape were interpreted as the shrinkage in volume resulting from the formation of the ordered phase. They were smaller at aging temperatures below 673 K. The change in microstructure was different at aging temperature between 573 and 773 K. These phenomena were due to the following: First, nodules which nucleated at the grain boundaries would have grown into the grains well before the formation of the ordered phase, and second, the shrinkage in volume would have been of a different kind that the ordered phase. Therefore aging treatments should be carried out at low temperature in order to minimize the changes in shape. Also, nodular growth should be inhibited in order to maximize the hardening in Ag-rich alloys.

Alloys↗
PubMedDate not supplied

Autosomal dominant congenital cataract and microphthalmia associated with a familial t(2;16) translocation.

We describe a family in which autosomal dominant congenital cataract and microphthalmia were segregating together with a reciprocal translocation t(2;16) (p22.3;p13.3) through three generations. This family included four individuals with balanced translocations, three with partial trisomy 2p derived from this translocation, and two with a normal karyotype. All of the subjects with balanced and unbalanced translocations had congenital cataract and microphthalmia, whereas the two individuals with normal karyotypes did not show any ocular anomalies. These observations suggest that the altered function of a gene that lies on the 16p13.3 band and that has an important role in the development of the eye is responsible for this disorder.

Abnormalities, Multiple↗
PubMedDate not supplied

Percutaneous transluminal angioplasty in segmental obstruction of the hepatic inferior vena cava: long-term results.

Percutaneous transluminal angioplasty (PTA) was performed in 8 patients with segmental obstruction of the hepatic inferior vena cava. Using Grüntzig balloon catheters, canalization and dilatation were initially successful in all 8 patients without any complications. In long-term observation of 12-48 months, recurrence of obstruction was confirmed in 5 patients. These were recanalized easily by repeat PTA. Long-term patency was obtained in 3 patients in whom the lumen was opened to 13 mm or more in diameter as seen in lateral views of the vena cavogram.

Adult↗
PubMedDate not supplied

Ischemic neuronal damage. How does mild hypothermia modulate it?

The significance of mild hypothermia as a therapeutic measure for ischemic brain damage is presented on the basis of different experimental results. An extracellular glutamate surge, a sustained activation of N-methyl-D-aspartate (NMDA) receptors, and an enhancement of DNA binding activity to transcription factor AP-1, all being key items directly linked to excitotoxic neuronal damage, are deeply affected by slightly lowering temperature (mild hypothermia [MH]). The cellular mechanism of MH seems rather nonspecific but tends to collectively involve these key items rendering neurons resistant to ischemic damage. Clinical application of MH should be a great challenge to relieve deadly effects on central neurons.

Animals↗
PubMedDate not supplied

Xenogeneic (pig to rat) fetal liver fragment transplantation using macrocapsules for immunoisolation.

Acute liver failure caused by viral infection, surgical resection of a large part of the liver or by drug use has a high mortality. For its treatment, hepatocyte or liver tissue transplantation is useful. We report here the beneficial effects of xenogeneic fetal liver fragment (FLF) transplantation with an immunoisolation macrocapsule. The macrocapsules were made of a microporous polypropylene membrane. Pig FLFs (1 mL) was inserted into each capsule to serve as a graft in LEW rats. Acute liver failure was induced by 90% liver resection on day 0. Group 1: transplantation of encapsulated FLF into the omentum 2 days before liver resection (n = 17). Group 2: FLF transplantation into the omentum on day -2 (n = 11). Group 3: liver resection (control) (n = 19). The survival rate, the histology of the grafts and the biochemical parameters [blood sugar (BS), GPT, and GOT] were evaluated. The survival rates of groups 1, 2, and 3 on day 7 were 70.6, 0, and 11.1%, respectively. There were significant differences in BS, GPT, and GOT levels between groups 1 and 3 on day 1 (p < 0.05). On day 28, the histological analyses of the grafts of encapsulated FLFs revealed that the hepatocytes appeared viable, but that the haematopoietic cells had degenerated. Xenogeneic FLFs with macrocapsules survived more than 1 mo, and supported the host's liver function.

Animals↗
PubMedDate not supplied

Extensive proliferation of mature connective-tissue type mast cells in vitro.

There are two phenotypically distinct subpopulations of mast cells in rodents: connective tissue-type mast cells (CTMC) and mucosal mast cells (MMC). These populations differ in their location, cell size, staining characteristics, ultrastructure, mediator content and T-cell dependency. Several investigators recently reported a further subclass of mast cells which arise when normal mouse haematopoietic cells are cultured with interleukin-3 (IL-3); IL-3 is an activity similar or identical to mast-cell growth factor, histamine-producing factor, or P-cell stimulating factor. These cultured mast cells are in many ways similar to MMC; they stain with Alcian blue but not safranin, contain chondroitin sulphate E proteoglycan rather than heparin proteoglycan and have relatively low histamine content, as do MMC. Although proliferation of MMC is known to be T-cell dependent in vivo and thought to be IL-3-dependent in vitro, the factors on which CTMC proliferation depends remain elusive. Here we show that mature CTMC purified from mouse peritoneal cells can proliferate in vitro in methylcellulose culture and maintain the appearance and function of CTMC. We also present evidence that mature CTMC cannot proliferate in the presence of pure IL-3 alone.

Animals↗
PubMedDate not supplied

Microencapsulation of pesticides and their improved handling safety.

The purpose of pesticide formulations and recent requirements of pesticide industry are discussed, and the importance of research work on pesticide delivery systems, is pointed out. For this purpose, the microcapsules, as one of the typical controlled release formulations, play an important role. Handling safety of the pesticides is improved considerably by microencapsulation due to hazard and exposure reduction.

Agriculture↗