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Biomedical subjects

K Tsuji

Publications and source records attributed to K Tsuji.

At least 793 records · Page 44Linked to original sources
PubMed1979

HLA in hypertrophic cardiomyopathy and rheumatic heart disease.

1. HLA antigens were determined in 30 Japanese patients with hypertrophic cardiomyopathy. Several antigens were more common in patients compared with controls, but statistically significant differences were not evidenced. In families one and two, six of seven kindred who inherited HLA-A9 and B7 had the disease. None of five kindred lacking HLA-B7 showed evidence of the disease. In families three and four, affecting family members had HLA-A2 and BW-35. Our finding suggest that the HLA system may play some role in the pathogenesis of hypertrophic cardiomyopathy with familial occurrence. 2. Twenty patients with rheumatic valvular heart disease were also studied. There was no significant difference in frequencies of HLA antigens between patients and controls.

Adolescent↗
PubMed1979

[In vivo distributions of 111In and/or 3H labeled lymphocyte in C3H/He mouse (author's transl)].

Although 99mTc and 51Cr have been used for lymphocyte labeling, these radionuclides have several disadvantages for study on immunological behaviour of lymphocyte; very high rate elution and low labeling efficiency for both radionuclides, and short half life for 99mTc. Indium-111 has quite suitable physical properties for clinical nuclear medicine, i.e. desirable photon energy (247,173 keV) and 2.8 day half life. 111In-oxine is lipid soluble and is known to pass through the cell membrane and attaches firmly to cytoplasmic component of the cell. On the other hand, 3H-thymidine is well known substance which incorporated to nucleic acid in the cell. In this study, distribution patterns of 111In-oxine and/or 3H-thymidine labeled lymphocyte in C3H/He mice were examined and the suitability of 111In-oxine labeled lymphocyte for radionuclide imaging in vivo was discussed. Thirty minutes after intravenous injection of 3H and/or 111In labeled lymphocyte, about 12% of lymphocyte were found in the lungs and rest of them were distributed mainly in the blood, kidneys and liver. After 24 hours the activity in the lung decreased markedly and the activity in the liver and kidneys increased with time. Between lymphocyte labeled with 111In-oxine and 3H-thymidine, there is not so much differences in terms of distribution patterns. From this study, it is concluded that the 111In-oxine labeled lymphocyte distributes in the same way as 3H labeled one, in spite of different labeling sites. This 111In-oxine labeling method can be used as a useful tool of radionuclide imaging in kinetic studies of lymphocyte in vivo.

Animals↗
PubMed1979

Application of LAL for detection of endotoxin in antibiotic preparations.

The Limulus amebocyte lysate (LAL) test has been investigated as an alternative method to the USP pyrogen test for the detection of endotoxin contamination in these antibiotic preparations: clindamycin phosphate, lincomycin hydrochloride, neomycin sulfate, and spectinomycin hydrochloride. The antibiotic preparations were tested at the maximum concentrations that would not inhibit gelation of LAL. The USP pyrogen test was also performed for comparison. The LAL and the USP pyrogen tests correlated extremely well when an acceptance criterion based on the minimum pyrogenic dose (MPD) of endotoxin per human dose of drug was used for the LAL test. Use of this MPD provides a practical and feasible acceptance creiterion for the LAL test and assures that the LAL sensitivity approximates or surpasses that of the USP pyrogen test.

Animals↗
PubMed1979

Limulus amebocyte lysate - a means to monitor inactivation of lipopolysaccharide.

A properly selected Limulus Amebocyte Lysate (LAL) has been demonstrated to have a good correlation with the in vivo United States Pharmacopeial pyrogen test when testing lipopolysaccharide (LPS) inactivated with ethylene oxide, 60 Co irradiation, and dry-heat treatments. Thus, LAL may be used to determine degradation or depyrogenation kinetics of LPS. A criteria for acceptance of an LAL lot for correlation with the USP pyrogen test is described.

Animals↗
PubMed1979

Further study on HLA-A, B, C, D, DR and haplotype antigen frequencies in psoriasis vulgaris.

HLA-A, B, C, D, and DR antigens in 40 Japanese patients with psoriasis vulgaris were analysed using the antisera obtained from the Seventh International Histocompatibility Workshop. The most deviant HLA antigens in psoriasis were found to be HLA-Cw6 and DRw7. by observing the HLA-A, B, C and DR antigens en bloc in psoriasis, it is shown that the statistically significantly associated haplotype antigens were HLA-A1, B13, Cw6 and DRw7, and A1, B37, Cw6 and DRw6 respectively. No HLA-Dw11 (LD17) antigen was associated with the disease.

Epitopes↗
PubMed1978

Fluorimetric determination of erythromycin and erythromycin ethylsuccinate in serum by a high-performance liquid chromatographic post-column, on-stream derivatization and extraction method.

The method described is capable of detecting less than 0.01 microgram/ml of erythromycin and/or erythromycin ethylsuccinate in serum. Recoveries of erythromycin and erythromycin ethylsuccinate, when added to the level of 0.6 microgram/ml, were 102 and 97% with relative standard deviations of 6.0 and 4.5%, respectively. Erythromycin ethylsuccinate in serum at 37 degrees was shown to degrade rapidly to erythromycin in a first-order rate. When stored at -20 degrees, however, only 10% of the erythromycin ethylsuccinate in serum was hydrolyzed even after storage for 36 days. The method was used to analyze erythromycin and erythromycin ethylsuccinate in sera from ten subjects administered with an oral dose of erythromycin ethylsuccinate. In addition to a small amount of anhydroerythromycin ethylsuccinate and 8,9-anhydro-6,9-hemiketal erythromycin ethylsuccinate, at least two other metabolites were detected in sera and one was tentatively identified as erythralosamine.

Chromatography, High Pressure Liquid↗
PubMed1978

High-performance liquid chromatographic determination of erythromycin.

An isocratic, reversed-phase high-performance liquid chromatographic (HPLC) method has been developed for the determination of erythromycin. The method uses a muBondapak C18 reversed-phase column with a mobile phase composed of acetonitrile-methanol-0.2 M ammonium acetate-water (45:10:10:35). The relative standard deviation of the method for quantification of erythromycin is about 0.6%, and the method is capable of selectively quantifying erythromycins A, B, C and at least nine other erythromycin epimers and degradation compounds in 15 min of chromatographic time. The bio-equivalent potencies of erythromycin powders calculated from the HPLC data agreed well with those of the microbiological assay method. The HPLC method developed is also applicable for the analysis of various derivatives of erythromycin.

Chromatography, High Pressure Liquid↗
PubMed1978

Evaluation of acceptance criteria for particulate limits for small-volume parenteral products.

The precision of and correlation between the USP membrane filtration-microscopic method and the instrumental method for sizing and quantifying particulate matter in small-volume parenteral products were determined using simulated products. The total variance for the instrumental counts was lower than the USP method for all products in the 10--25-mum particle range and for most products in the greater than or equal to 25--50-mum range. A linear relationship between the instrumental counts and the USP counts was demonstrated for the 10--25-mum particle range. However, the instrumental reading was higher than the USP method for counts of 10 or more particles/ml. The instrumental and the USP methods failed to correlate on particulate sizes greater than 25 mum. The content of particulate matter in over 100 small-volume parenteral products was sized and quantified by the USP and the instrumental methods. From the instrumental data, a statistical treatment for the analysis of particulate data is presented as an objective method of evaluating acceptance criteria on particulate matter in small-volume parenteral products.

Autoanalysis↗
PubMed1978

Possible associations between HLA antigens and the immune responsiveness to attenuated rubella vaccine.

We investigated the possible associations between HLA antigens and the antibody response patterns during a clinical trial with a live attenuated rubella vaccine in 172 Japanese schoolgirls. Those vaccine recipients were divided into three groups, 42 low responders, 102 intermediate responders and 28 high responders, according to convalescent-phase hemagglutination-inhibiting antibody titers. High frequencies of HLA--A11 and HLA--B15 in high responders and HLA--Aw24 and HLA--B5 in low responders were found. Among HLA--A antigens HLA--A11 had the highest geometric mean HI antibody titer and HLA--Aw24 had the lowest. Among HLA--B antigens HLA--B15 had the highest and HLA--B5 had the lowest. Strong linkage disequilibrium was found between HLA--A11 and HLA--B15 in high responders and between HLA--Aw24 and HLA--B5 in low responders. These results suggest that there may be a relationship between HLA antigens and specific immune response genes in man as well as in other vertebrates.

Adolescent↗