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Biomedical subjects

K Tsuji

Publications and source records attributed to K Tsuji.

At least 667 records · Page 37Linked to original sources
PubMed1985

Relationship between human oocyte maturation and different follicular sizes.

The relationship between the follicular size in the human ovary and oocytes capable of resuming meiosis in vitro was examined in each phase of the menstrual cycle. Intact healthy oocytes with corona cells obtained from small (3-4 mm diameter), medium (5-8 mm diameter) and large (9-15 mm diameter) antral follicles were cultured at 37 degrees C for 43 h. At the end of the culture period the denuded oocytes were examined morphologically to determine whether the resumption of meiosis had occurred. In the follicular phase, the percentage of oocytes resuming meiosis (polar body extruded: PB) in the large-follicle group was significantly higher than that in the small-follicle group (P less than 0.05). The incidence of oocyte maturation including only nuclear maturity seemed to increase as follicles increase in size. However, in the luteal phase the incidence was fairly constant, irrespective of the follicular size. These results suggest that the capacity of human oocyte maturation is closely correlated with follicular maturation.

Adult↗
PubMed1985

Intravascular bronchioloalveolar tumor. Report of an autopsy case and review of literature.

An autopsy case with intravascular bronchioloalveolar tumor occurring in a 51-year-old Japanese woman is reported. The lungs contained multiple nodules which were characteristic features of this tumor. In addition, the tumor diffusely involved bilateral lower lobes associated with extensive pleural invasion. And the tumor tended to spread along vascular and bronchial trees. No extrathoracic metastasis was noted. Histologically, the nodules showed hypocellular hyaline or myxoid tissue surrounded by peripheral cellular layers. Diffuse consolidated areas were composed of collagenous connective tissue with scattered tumor cells. Factor VIII-related antigen could not be demonstrated in tumor cells, but the negative result was considered to be secondary to antigen deterioration by delayed fixation of the tissue. Electron microscopic examination did not support the alveolar epithelial cell origin, but revealed morphological characteristics of myofibroblasts and endothelial cells. The tumor is presumed to be derived from vascular stem cell with a tendency of endothelial differentiation.

Female↗
PubMed1985

Effect of dietary taurine on cholesterol gallstone formation and tissue cholesterol contents in mice.

The inhibitory effect of dietary taurine on cholesterol gallstone formation was studied using male mice of Jcl:ICR strain. Mice were provided three kinds of semi-purified diet; a cholesterol-free diet (standard), a lithogenic diet containing 0.5% cholesterol and 0.25% sodium cholate (C-CA), and a lithogenic diet supplemented with 5% taurine. The changes of total cholesterol in serum, cholesterol mass in the liver and the gall bladder, and gallstone incidence were studied as a function of time. Gallstone formation was observed only in the mice fed on the C-CA diet for more than 3 weeks. The changes of serum cholesterol concentration were not consistent with gallstone formation. The cholesterol mass of the liver in taurine-supplemented mice decreased after the 3rd week. Cholesterol content of the gall bladder increased with cholesterol gallstone formation. Therefore, the inhibitory effect of dietary taurine on cholesterol gallstone formation may be related to the decrease in cholesterol content of the liver.

Animals↗
PubMed1985

Brain nicotinic acetylcholine receptors. Biochemical characterization by neosurugatoxin.

Specific [3H]nicotine binding to rat forebrain membranes was saturable and of high affinity, and it exhibited pharmacological specificity as well as stereoselectivity for nicotinic agents. There was a regional variation of specific [3H]nicotine binding in rat brain. Low concentrations of neosurugatoxin markedly inhibited specific [3H]nicotine binding in rat forebrain (IC50 = 78 nM) and the competition curve by the toxin was biphasic (pseudo-Hill slope, 0.44). Approximately 50% of [3H]nicotine binding in rat forebrain was inhibited by low concentrations (0.3-100 nM) of neosurugatoxin and the residual binding was inhibited by higher concentrations (0.3-10 microM). In the presence of 3 nM, 100 nM, and 1 microM neosurugatoxin, there was a concentration-dependent (28, 54, and 71%, respectively) loss of [3H]nicotine-binding sites (Bmax) in rat forebrain with little change in the dissociation constant (Kd). The blockade of brain [3H]nicotine-binding sites induced by neosurugatoxin was not reversed by washing. Further, the toxin (10 microM) considerably accelerated the dissociation of [3H]nicotine from its receptor sites initiated by nonlabeled (-)nicotine. These observations suggest that neosurugatoxin may allosterically regulate [3H]nicotine binding rather than competing directly. In contrast to a marked inhibition of [3H]nicotine binding, neosurugatoxin (100 nM-10 microM) had no effect on brain [3H]quinuclidinyl benzilate binding. In conclusion, the present study has shown that [3H]nicotine selectively labels nicotinic cholinergic receptors in rat brain and that neosurugatoxin is a potent noncompetitive antagonist of these receptors.

Animals↗
PubMed1985

Biological significance of Ss (serum soluble) HLA-class I antigens in bone marrow transplantation.

Ss (serum soluble) HLA-class I antigens were investigated in 10 cases of bone marrow transplantation and the relationships among Ss HLA antigens, lymphocyte counts, GvHD and the clinical course were compared. Cs (cell surface) HLA antigens were typed by the NIH standard microcytotoxicity test. Measurement of Ss HLA antigens was performed by both the lymphocytotoxicity inhibition test and solid phase RIA. A good correlation was found between the cytotoxicity inhibition test and solid phase RIA. No correlation was observed between lymphocyte counts and changes in the Ss HLA antigens in the presence of GvHD. In cases with GvHD, Ss HLA antigens were found to correlate with the GvHD. Ss HLA antigens also correlated with levels of lymphocyte counts in cases without GvHD. Ss HLA antigens are an important marker of biological significance in GvHD following bone marrow transplantation.

Adolescent↗
PubMed1985

Isolation and characterization of the cDNA clone and genomic clones of a new HLA class II antigen heavy chain, DO alpha.

From a human cDNA library constructed from a consanguineous HLA-homozygous cell line, AKIBA (HLA-A24, Bw52, DR2, Dw12, DQw1, and Cp63) (Cp63, a new SB type), a cDNA clone encoding a new HLA class II antigen heavy chain named DQ alpha was isolated, and was analyzed by Southern blot hybridization and by nucleotide sequence determination. The nucleotide sequence of the DO alpha cDNA was distinct from those of the DR alpha, the DQ alpha, and the DP alpha cDNA, but showed some characteristic features of the class II antigen alpha-chains. We also isolated and identified genomic clones specifying the DO alpha gene. Genomic analyses of cell lines with different HLA-DR serotypes with the use of the DO alpha cDNA as a probe indicated the existence of a single DO alpha gene that exhibited little restriction enzyme polymorphism.

Amino Acid Sequence↗
PubMed1985

Changes in serum levels of pancreatic isoamylase, lipase, trypsin, and elastase 1 after endoscopic retrograde pancreatography.

To compare the behavior of pancreatic enzymes in the circulation, serum levels of pancreatic isoamylase activity, lipase activity, immunoreactive trypsin (IRT), and immunoreactive elastase 1 (IRE) were measured in the same serum samples taken serially from 29 subjects undergoing endoscopic retrograde pancreatography (ERP). A striking correlation between the maximal increments of serum pancreatic enzyme levels after ERP and the degrees of opacification of pancreatic duct system was observed, except in 6 subjects with chronic pancreatitis. In 13 out of 19 subjects whose main pancreatic duct (MPD) and branches were opacified, serum pancreatic enzyme levels reached a peak within 2 hours after ERP and decreased thereafter. The mean maximal rise of serum levels of lipase, IRT, pancreatic isoamylase, and IRE in the 13 subjects was 32, 21, 10, and 4 times the basal value, respectively. A delay of peaking in serum levels of pancreatic isoamylase and IRE as compared with those of lipase and IRT was observed in the 13 subjects. The mean disappearance half-time of serum lipase, IRT, pancreatic isoamylase, and IRE in the 13 subjects was 2.8, 4.6, 8.0 and 16.0 hours, respectively.

Adolescent↗
PubMed1985

Experimental study of fetal liver transplantation.

Fetal liver cells function as liver and bone marrow cells. The direct and indirect effects of fetal liver cell transplants on liver and/or bone marrow failure was investigated in rat experiments. Outbred Wistar, inbred Lewis and F344 fetus rats (18-20 days) were used as donors; Wistar and Lewis rats (210-280g) were used as recipients. D-galactosamine (1.0-1.85g/kg) was administered intraperitoneally inducing acute liver failure. TBI, Co60 1000 Rads, was given inducing bone marrow failure. Donor liver cells were prepared using the following four methods: 1) simple liver homogenation; 2) centrifugation by percol gradient into different fractions; 3) arginine deficient culture; and 4) pronase culture. Preliminary observations of the transplant effect of fetal liver cells on liver and/or bone marrow failure is described.

Acute Disease↗