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Biomedical subjects

K Terasaki

Publications and source records attributed to K Terasaki.

34 records · Page 2Linked to original sources
PubMed1992

Electrophoretic analysis of a natural population of the Thai Paragonimus heterotremus and its genetic relationship to the three Japanese species P. miyazaki, P. ohirai and P. westermani.

A total of 18 enzymes (encoded by 20 loci) from Paragonimus heterotremus collected at a local area in Saraburi, Thailand, were electrophoretically compared with those from the three Japanese species P. ohirai, P. miyazaki and P. westermani using horizontal starch-gel electrophoresis. Relative genetic distances between these species were quantified. The Thai P. heterotremus was found to be most closely related to P. miyazaki and rather distantly related to P. westermani. On the other hand, among the three Japanese species, the closest relationship was observed between P. miyazaki and P. ohirai.

Alleles↗
PubMed1980

Properties of arginase from liver of Macaca fascicularis; comparison of normals with red blood cell arginase deficient monkeys.

Deficiency of arginase (E.C. 3.5.3.1), the fifth enzyme of the urea cycle, was found in the red blood cells (RBCs) of Macaca fascicularis monkeys (less than 0.2 micromol arginine cleaved/g Hb/min; normal equals 49.2). Liver biopsies were obtained from two of these monkeys and from one monkey with normal levels of RBC arginase activity. Arginase from both groups of animals required Mn2+ for maximal enzyme activity and demonstrated a pH optimum of 10.2 in vitro. The activity of arginase in the livers of all three monkeys was 1.1 millimol arginine cleaved per g protein per min. The apparent Km for arginine of arginase in the livers of the RBC-deficient monkeys was 7.4 and 5.9 mM and in the normal monkey was 6.9 mM. Similar patterns of heat denaturation was seen at 69 C without Mn2+ present and 79 C in the presence of 20mM Mn2+. No difference in mobility on either RBC-deficient or normal monkeys was found. In addition, liver arginase from all three monkeys reacted similarly with anti-human liver arginase antibody. Liver arginases in RBC-deficient and normal monkeys were identical by ten criteria. These studies do not distinguish among several hypotheses for the genetic determination of arginase in different organs of this species and of man.

Amino Acid Metabolism, Inborn Errors↗
PubMed1978

[Phylogenetic studies on some Japanese amphibia by means of immunoelectrophoresis. II. Relationships of some amphibians to Japanese pond frogs (author's transl)].

Since the Japanese pond frogs (Rana nigromaculata and R. brevipoda) are known to be very closely allied with each other in morphological, ecological, physiological or immunological characters, the phylogenetical relationships between the Japanese pond frogs and other 10 species of Japanese amphibians were investigated by means of immunoelectrophoretic analysis of liver extract. The results obtained are as follows: (1) There are conservative antigens which are commonly found in all species of Anura. (2) The Japanese pond frogs have specific antigens. (3) R. nigromaculata and R. brevidpoda are very closely allied with each other. (4) Four species of the genus Rana (R. rugosa, R. catesbeiana, R. ornativentris and R. japonica) are closely related to the Japanese pond frogs. (5) Two species of the genus Rhacophorus (Rh. arboreus and Rh. burergeri) are related to the Japanese pond frogs. (6) R. limnocharis is related to the Japanese pond frogs at the same extent as the genus Rhcophorus is. (7) Tow species of the suborder Procoela (Hyla arbored and Bufo bufo) are only partially related to the Japanese pond frogs. (8) Cynops pyrrhogaster pyrrhogaster of Urodera had only a few common antigens with the Japanese pond frogs.

Amphibians↗
PubMed1975

[Phylogenetic studies on some Japanese amphibia by mean of immunoelectrophoresis. I. Relationships of some frogs and newt to toad (author's transl)].

The phylogenetic relationship among toad, frogs and newt from Japan was investigated by means of immunoelectrophoresis. Homologous, heterologous and non-identity reactions between rabbit anti-Bufo bufo antiserum and antigen from each of 13 species and 2 subspecies of Japanese amphibia were examined. The number and positions of arcs appeared in these reactions were compared. The relationship observed in the comparison well coincided with the classification of the upper taxa of amphibia; viz 1) Hyla arborea is more intimately related to B. bufa, 2) members of Ranidae and Rhacophoridae have equal intimacy for B. bufo, and 3) Cynops pyrrhogaster is most distantly related to B. bufo. By contrasting arc obtained in non-identity reaction and those obtained in homologous reaction, it was found that toad, B. bufo carried 22 antigens, and among these antigens, one was observed through all amphibians tested, 12 were carried solely by toad, and some of the remainders were common to antigens carried by various frogs. Newts carried only one antigen common to toad. Based on these results, the phylogeny of Japanese amphibia was discussed.

Amphibians↗