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Biomedical subjects

K Teramoto

Publications and source records attributed to K Teramoto.

At least 73 records · Page 4Linked to original sources
PubMed1992

31P NMR assessment of orthotopic rat liver transplant viability. The effect of warm ischemia.

The relationship between NMR visible high energy phosphates and transplant outcome for the case of liver damage by warm ischemia was investigated. In vivo 31P nuclear magnetic resonance (NMR) spectroscopy of rat liver was performed before the induction of warm ischemia in the donor and 20 min after reestablishment of portal blood flow in the recipient. Pretransplant damage was varied by subjecting the livers to 0, 15, 30, or 60 min of warm ischemia prior to harvesting. In the controls (0 min warm ischemia), 4 of 4 rats survived transplantation (one week survival end-point) and the mean NTP recovery was 94 +/- 8%; 3 of 6 rats survived in the 15 min warm ischemia group. Mean NTP recovery was 77 +/- 20% in the 15 min survival subgroup and 32 +/- 20% in the nonsurvival subgroup. Of 6 rats, 1 survived in the 30 min group. NTP recovery was 44% for the 30 min survivor and 37 +/- 5% in the nonsurvival subgroup. Of 4 rats, 1 survived in the 60 min warm ischemia group. NTP recovery was 56% for the 60 min survivor and 28 +/- 7% in the nonsurvival subgroup. Overall, there was a significant difference between the mean NTP recovery of the survival and nonsurvival subgroups (78 +/- 21% versus 31 +/- 18%, P < 0.001). The dividing line between the survival and nonsurvival groups was approximately 50% NTP recovery. Of 9 rats with liver NTP recovery greater than 50%, 8 survived while 10 of 11 rats with less than 50% recovery died. NMR visible NTP recovery 20 min after the reestablishment of portal blood flow was a good indicator of transplant outcome in the case of rat liver damage by warm ischemia.

Animals↗
PubMed1992

Serum immunoglobulin and complement C3 levels in workers exposed to lead.

Serum immunoglobulin and complement C3 levels were measured in workers exposed to lead at a secondary lead refinery and a solder factory. In the first survey, significant correlations were found between blood lead and IgA with a correlation coefficient of 0.296, and between blood lead and IgE with a correlation coefficient of 0.314. No other significant correlations were found among the indicators of lead exposure and humoral immunity. In the second survey, no significant correlations were found between blood lead and IgG and IgA. A significantly higher number of subjects with IgE of more than 400 IU/ml was found in the group with blood lead of more than 60 micrograms/100 g.

Adolescent↗
PubMed1991

Localization and ultrastructure of the Kupffer cells in orthotopically transplanted liver grafts in the rat.

Kupffer cells play an important role in the acceptance or rejection of liver grafts. We examined the ultrastructure of the Kupffer cells in transplanted rat livers, from an early to a late stage where the graft is accepted, using a DA-to-PVG combination. Two days after surgery, endocytic activity of the Kupffer cells had increased, as evidenced by worm-like structures and many endocytic vacuoles. There was often close apposition to the monocytes or lymphocytes. By day 4, infiltration of mononuclear cells into the sinusoids was readily noticeable. By day 7, several Kupffer cells had migrated into the space of Disse through the openings in endothelial linings. The number of Kupffer cells reached a maximum at 14 days. They were located mostly outside the sinusoid, adhering to the hepatocytes. At this point in time, however, the Kupffer cells contained few endocytic vacuoles and phagolysosomes, in contrast to those at 2 days. The number and location of Kupffer cells became almost normal at 2 months. The present results indicate that Kupffer cells are highly activated before mononuclear cell infiltration becomes manifest in the sinusoid, and that when a rejecting reaction reaches a peak, they are usually located extrasinusoidally and show a morphologically immature profile.

Animals↗
PubMed1991

Early diagnosis of acoustic neuroma (1989) after experience of 37 cases.

Although the diagnosis of acoustic neuroma as classically described is generally thought to be easy, this is not always true for this so-called "ear tumor" which is localized within the internal auditory meatus. Recently, remarkable progress has been made in the diagnostic instrumentation and operative techniques used to treat acoustic neuroma, emphasizing the need for early diagnosis so that treatment can take place earlier than before. Through early diagnosis, some cases of total resection of acoustic neuroma have recently become possible, with preservation of not only facial nerve function, but preservation of auditory function as well. In the present report, we outline the early diagnosis of acoustic neuroma by summarizing 37 cases of acoustic neuroma experienced at our institution. We discuss historical presentations, and laboratory tests used in diagnosis including pure tone audiometry, speech audiometry, auditory brainstem evoked response (ABR), vestibular caloric stimulation, and computed tomography (CT) enhanced by introduction of air into the posterior fossa. Two representative cases are also presented. It should be emphasized that no clinical test is 100% sensitive or specific, and attention must be paid to chronological discrepancies in the patient's presentation and "cookbook"-type approaches to evaluation should be avoided.

Adult↗
PubMed1991

Visual suppression test using post-rotatory nystagmus. Clinical course in patients with motion sickness.

With the results of VS test (%) we were able to classify patients into three groups as follows: Group I, hyper range of visual suppression, VS (%) = 100-90; Group II, normal range of VS (%) = 89-45; Group III, reduced or abolished range of VS (%) = less than 44. The results with VS test using post-rotatory nystagmus (PRN) were in Group I 58 cases, in Group II 28 cases and in Group III 1 case, and using caloric nystagmus (CN) the results in Group I were 39 cases, Group II 29 cases, and Group III 1 case. With the correlation of VS test between PRN and CN we were able to classify patients into four types as follows: Type I, hyper range of VS in both PRN and CN; Type II, hyper range of VS in PRN; however, normal range of VS in CN; Type III, normal range of VS in both PRN and CN; Type IV reduced or abolished range of VS in both PRN and CN. It was very interesting in the clinical course of motion sickness that patients under 11 years of age (18 cases) of Type I (31 cases) showed marked improvement to Type III (11 cases) at the time of disappearance of symptoms; however, patients over 12 years of age (13 cases) of Type I remained stationary Type I (9 cases) at the time of disappearance of symptoms.

Cerebellum↗
PubMed1991

Erythrocyte deformability in workers exposed to lead.

Erythrocyte deformability and other hematological indicators were determined in 17 male workers exposed to lead at a secondary lead refinery and 13 controls. Blood lead, urine lead, urine coproporphyrin, delta-aminolevulinic acid and erythrocyte zinc protoporphyrin were determined to evaluate the degree of lead exposure in the lead workers above. For the measurement of erythrocyte deformability, the microfilter method was used. The results were summarized as follows: 1. The mean values of blood lead, urine lead, urine coproporphyrin, urine delta-aminolevulinic acid and erythrocyte zinc protoporphyrin levels in lead workers were 53.5 micrograms/100g, 141.4 micrograms/l, 115.9 micrograms/l, 12.0 mg/l and 68.9 micrograms/dl respectively, suggesting a moderate influence of lead exposure. 2. The mean values of erythrocyte count, hematocrit and hemoglobin were significantly lower in lead workers than those in controls. No significant differences were found in the mean values of mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration and corpuscular natrium and potassium between lead workers and controls. 3. Erythrocyte deformability was significantly reduced in lead workers compared with controls.

Adult↗
PubMed1990

Biological monitoring of workers exposed to lead stearate.

This study was conducted to examine the usefulness of some of the commonly used biological parameters for monitoring of workers exposed to lead stearate. Forty-two lead stearate workers from a lead stabilizer factory and 26 workers exposed to inorganic lead compounds were involved in this study. Although the workers had similar blood lead values (PbB), subjects exposed to lead stearate were found to have a significantly higher concentration of lead in plasma (PbP), 1.0 +/- 0.57 micrograms dl-1) than workers exposed to inorganic lead compounds (0.42 +/- 0.3). The ratio of PbP to PbB was ca. 2.5 times higher for lead stearate workers (0.38) than the inorganic lead workers (0.15). These data suggest that the different chemical properties of lead stearate may result in different distributional patterns of the metal in different blood components. On the other hand, the activity of delta-aminolevulinic acid dehydratase (ALAD), an enzyme highly sensitive to lead, was not so much depressed among the lead stearate workers as that of workers handling inorganic lead. A poor correlation was also observed between PbB and ALAD activity of the stearate workers. These findings indicate that PbB and ALAD are not good biological indicators for evaluating the toxicological effect of lead stearate exposure.

Chemical Industry↗
PubMed1990

DNA synthesis in hepatocytes during liver allograft rejection in rats.

We have investigated DNA synthesis in hepatocytes after orthotopic liver transplantation in four rat combinations--DA into DA (isogeneic), DA into PVG (allogeneic, nonrejector), DA into BN (allogeneic, rejector), and DA into (BN X PVG)F1 (allogeneic, intermediate nonrejector). The methods used were assay of thymidine kinase activity in graft homogenate and staining of hepatocytes in liver sections by an antibromodeoxyuridine monoclonal antibody. Both demonstrated increased DNA synthesis in grafts in all combinations except isogeneic, and showed that its intensity and timing paralleled the occurrence of the rejection reaction. The significance of hepatocyte regeneration after liver grafting is discussed.

Animals↗
PubMed1990

The in vitro and in vivo effects of lead on delta-aminolevulinic acid dehydratase and pyrimidine 5'-nucleotidase.

The in vitro and in vivo effects of lead on the activity of pyrimidine-5'-nucleotidase (P5N, E. C. 3.1.3.5) and delta-aminolevulinic acid dehydratase (ALA-D, E.C. 4.2.1.24) were studied. Incubation of blood with lead at concentrations of up to 3 mumol/l (about 60 micrograms/dl) did not appear to affect the activity of P5N, while the activity of ALA-D decreased dose-dependently with lead. Administration of lead caused a marked and rapid suppression of ALA-D in rats. The suppression of lead on P5N appeared to be a rather slow process. The decrease of its activity only came into effect 20 days after administration with lead. these findings suggest that lead induced P5N inhibition is a slow process while the suppression of ALA-D activity occurs much earlier.

5'-Nucleotidase↗