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Biomedical subjects

K Tauchi

Publications and source records attributed to K Tauchi.

At least 55 records · Page 3Linked to original sources
PubMed1988

Are fluorescent bodies of Y-spermatozoa detectable in common with mammalan species?

An attempt was made to detect the fluorescent bodies (F-body), using Quinacrine mustard (Q-M) staining in the spermatozoa from eight mammalian species (human, bull, boar, dog, rabbit, rat, mouse, and mastomys) as well as in the cock (used as negative control). Sperm suspension, prepared after rinsing by repeated centrifugation with phosphate buffered saline (PBS), was either stained with Q-M for 24 h or treated with protease and then stained with Q-M for 60 min. The final concentration of Q-M in the mixed staining sperm suspension was 0.025 mg/ml. The examination using a reflecting fluorescent microscope revealed that the F-body found in human sperm was also present in the sperm of all the mammals but not in the cock after 24 h of staining. The enzyme-treated specimens showed higher incidences of F-bodies than specimens stained for 24 h without enzymatic digestion. These findings strongly suggest that the F-body is commonly present in the spermatozoa of many mammalian species.

Journal Article↗
PubMed1988

[Improvement of the vomeronasal organ ablation in the rat].

The vomeronasal organ (VNO) in most mammalian species consists of a bilaterally paired tubular structure lying on either side at the base of the nasal septum. Functionally, the VNO receives chemosensory informations and conveys it to its parts of the hypothalamus thought to be important in the regulation of reproductive physiology and behavioral functions. Here the authors report an improvement of the VNO ablation in the rat. With the exception of restraint of a rat, this operation method is fundamentally similar to those reported by Saito and Mennella (1986). The rat was anesthetized and placed supine on a home-made operating board with some accessories, and its extremities and head were fixed. It should be emphasized that the restraint of its lower incisor and tongue to keep the mouth open was performed with home-made restraint devices. The above method enabled us to remove the VNO easily. In addition, it could be useful in this field of oral surgery in small animals.

Animals↗
PubMed1986

Teratogenicity study on 4-bromophenyl chloromethyl sulfone (BCS) in the rat.

To investigate the teratogenicity of 4-bromophenyl chloromethyl sulfone (BCS), doses of 0, 5, 35 and 245 mg/kg/day of the test substance were administered by oral gavage to Wistar-Imamichi rats from days 6 to 15 of gestation. The results were as follows: The treated dams did not exhibit any toxic symptoms or mortality attributable to the test substance. The following changes were noted in the treated dams: dose-dependent reduction of body weight gain and food intake in 35 and 245 mg/kg groups, and decreased water intake, reduced brain and thymus weights and increased relative weights of liver and kidney in the 245 mg/kg group. Fetal toxicity, increase of mortality and body weight depression, caused by the test substance was not observed. External, skeletal and visceral examination of the fetuses did not reveal any abnormalities ascribed to the test substance. With regard to ossification, the incidence of fetuses with delayed ossification of the 2nd and 5th sternebrae was significantly higher than in the control group. In summary, dams given BCS in doses of 35 mg/kg and 245 mg/kg displayed toxic manifestations. Fetuses showed slightly delayed ossification. However, the test substance was concluded to be free of any lethal or teratogenic effects.

Abnormalities, Drug-Induced↗
PubMed1984

[Teratogenicity study on bromperidol in rats].

A teratogenicity study on bromperidol, a neuroleptic, was carried out in Wistar-Imamichi rats. Bromperidol was administered orally at the dose levels of 0.2, 1.5 and 10.0 mg/kg/day for 11 days from day 7 to day 17 of gestation. About two-thirds of pregnant females in each group were sacrificed on day 21 of gestation, and their fetuses were examined. The remaining dams were allowed to litter naturally, and the post-natal development of the offspring was observed. Treatment of bromperidol caused sedation of pregnant females in 1.5 and 10.0 mg/kg groups with concomitant decrease in body weight and in food and water intake in dose-dependent manner. Effect of bromperidol treatment on F1 generation was observed mainly in animals of 10.0 mg/kg group, suggesting overall growth retardation such as decrease in live fetal weight, retarded ossification of metacarpals and metatarsals, a slight decrease in day 1 viability index, a marginal delay in eyelid opening, reduction in growth rate and decrease in organ weight. No treatment-related external and internal abnormalities were observed in fetuses (F1), and no apparent effect on behavior, learning ability and reproductive function of offspring (F1) was found.

Abnormalities, Drug-Induced↗