Search PubMed⌕ Search

Biomedical subjects

K Tani

Publications and source records attributed to K Tani.

At least 379 records · Page 21Linked to original sources
PubMed1983

[Case of simultaneous multiple primary lung cancer].

The incidence of multiple primary bronchogenic carcinomas has increased in Japan as the incidence of bronchogenic malignancy has increased. The patient was a 71-year-old man with simultaneous multiple bronchogenic carcinomas. He had large cell carcinoma in the left upper lobe and adenocarcinoma in the left lower lobe. We reviewed 66 reported Japanese cases with multiple lung cancers with special reference to sex distribution, age, simultaneous or metachronous, location, histological types, treatment and prognosis.

Adenocarcinoma↗
PubMed1983

Phosphoglycerate kinase abnormalities: functional, structural and genomic aspects.

Phosphoglycerate kinase (PGK) deficiency is associated with hemolytic anemia and mental disorders in man. Complete amino acid sequence of normal human PGK was determined and its three-dimensional structure could be deduced from that of horse and yeast enzymes. Specific amino acid substitutions of several PGK variants associated with clinical problems were elucidated, and their functional abnormalities were correlated to their structural abnormalities. Full-length cDNA clones for normal human PGK were isolated and it is possible to examine PGK variants at the genomic level.

Amino Acid Sequence↗
PubMed1983

[Clinical studies of cefoxitin in the field of internal medicine].

Ten patients with sepsis and pneumonia complicated by leukemia or lung cancer were treated with cefoxitin (CFX) at daily dose of 6 g. The following results were obtained. 1. Clinical effects of CFX were good in 5 patients, fair in 2 and poor in 3 with effective rate of 50%. 2. Out of 8 patients with sepsis, 5 showed good response to CFX and effective rate was 62.5%. 3. Bacteriological outcomes were eradicated in 1, unchanged in 1, replaced in 2 and unknown in 6 cases. 4. Diarrhea was observed in 1 patient but this was not considered related to CFX therapy. 5. No abnormal laboratory finding due to CFX was observed. 6. It should be considered that 6 g or more of CFX is given in case of severe infections, such as sepsis or pneumonia complicated by serious underlying diseases.

Adult↗
PubMed1982

A new glucose-6-phosphate dehydrogenase variant (G6PD Nagano) associated with congenital hemolytic anemia.

A new glucose-6-phosphate dehydrogenase (G6PD) variant associated with chronic nonspherocytic hemolytic anemia was reported. The patient, a 6-year-old Japanese male, was noticed to have hemolytic anemia soon after birth, and a diagnosis of G6PD deficiency was made at the age of 2. He had episodes of hemolytic crisis several times after upper respiratory infection. G6PD activity of the patient was 5.5% of normal. The enzymatic characteristics were examined when he was 5 years old, and his G6PD showed faster-than-normal electrophoretic mobility, low Km G6P, high Km NADP, low Ki NADPH, normal utilization of substrate analogues, heat instability, and a normal pH optimum curve. From these results, this was considered to be a new variant and was designated G6PD Nagano. Infection-induced hemolysis and chronic hemolytic anemia seem to be due to markedly impaired enzyme activity and thermal instability.

Anemia, Hemolytic, Congenital↗
PubMed1982

Overproduction of structurally normal enzyme in man: hereditary haemolytic anaemia with increased red cell adenosine deaminase activity.

The mechanism of red cell adenosine deaminase (ADA) accumulation in a case of hereditary haemolytic anaemia due to increased red cell ADA activity was investigated. ADA activity of the younger cells was twice that of the older cells. Rate of ADA synthesis in erythroid colony cells cultured from the patient's bone marrow cells was 11-fold greater than that from the normal. The accumulation of ADA in the patient seems to be due to the increased synthesis in precursors of red cells in spite of the increased degradation in peripheral blood.

Adenosine Deaminase↗
PubMed1982

Studies on a new antibiotic M-92 produced by Micromonospora. I. Taxonomy of M-92 producing Micromonospora and antibiotic production therefrom.

An isolate (strain MCRL 0404) producing a new antibiotic, M-92, was identified as a new strain of Micromonospora for which the name Micromonospora verruculosa sp. nov. was proposed. A water-infusion of dried sea tangle and dried shiitake was utilized for the production of M-92. When this strain was fermented in the medium containing this infusion, M-92 accumulated in the mycelium at about 10 times that in the broth at the peak level.

Anti-Bacterial Agents↗
PubMed1982

Studies on a new antibiotic M-92 produced by Micromonospora. II. Isolation and physicochemical properties of M-92 and its components.

A new antibiotic complex, M-92 was isolated from the whole fermentation broth of Micromonospora verruculosa MCRL 0404. The whole broth was mixed with talc and filtered. The filter cake thus obtained was extracted with acidic methanol to give a crude powder of M-92 complex, which was then separated into A (acidic) and N (neutral or weakly acidic) groups. The A group components are soluble in alkaline water (pH 8.5), while the N group components are not. These components were further separated into six major components designated VA-2, BA-4, BA-5, BN-1, BN-2 and BN-3 by silica gel column chromatography. Components with the letter "V" are reddish violet and those with "B" are blue. The IR and UV spectra of these components suggest that their chromophores may be the same or very closely related to one another. The molecular formula of BN-3 was determined to be C29H23NO9 by mass spectrometry. The results of various spectroscopies on BN-3 suggest that M-92 components consisted of chromophores in which juglone (5-hydroxynaphthoquinone) is conjugated with naphthazarin (5,8-dihydroxynaphthoquinone).

Anti-Bacterial Agents↗
PubMed1982

Studies on a new antibiotic M-92 produced by Micromonospora. III. Biological activities.

The six major components of M-92, a new antibiotic complex produced by Micromonospora verruculosa MCRL 0404 showed a similar type of antimicrobial spectrum. Among these components, VA-2 exhibited the most potent antimicrobial activity, particularly significantly against some Gram-positive bacteria and Neisseria. VA-2 and BN-1 also exhibited marked inhibitory effects against L-forms of Staphylococcus aureus 209P and Mycoplasma. The MICs of VA-2, BA-4 and BN-1 were remarkably affected by the pH of the test medium, the inoculum size and the amount of horse serum added in the medium. By intraperitoneal administration, these components showed good protective effects in mice infected intraperitoneally with Staphylococcus aureus Smith. However, the protective effect decreased remarkably by other administration routes. In addition, components such as VA-2 and BN-1 exhibited cytotoxicity against HeLa S-3 cells in vitro and excellent in vivo antitumor activity against Ehrlich carcinoma. VA-2 possessed a high order of acute toxicity to mice [LD50:1.9 mg/kg (i.p.); 1.8 mg/kg (i.v.)], but others were relatively less toxic.

Animals↗
PubMed1982

[Studies of blood flow through the spinal cord of rabbit using the H2 clearance method ].

Using the H2 clearance method, spinal cord blood flow of the rabbit could be steadily measured. This allowed examination of blood flow problems using data analysis to provide informed decisions as to whether or not the blood flow through the gray and white matter was distinguishable. Spinal cord blood flow was also compared through the cervical, thoracic, and lumbar cord regions. The results were as follows: 1. The H2 clearance curves consisted of about 70% monoexponential curve (Mono), and 30% biexponenial curve (Bi), with a tendency toward higher values in the latter. 2. In Bi, a significant positive correlation was noted between the total flow values and the initial slope method values. The initial slope method was therefore used for subsequent data analysis. 3. By using a histogram to evaluate the data on each medullary segment, it was possible to separate a subgroup with a peak around 20 ml/100 g/min and another one with a peak around 40 ml/100 g/min. Based on the microangiographic findings, a more abundant vascular distribution was demonstrated in the gray matter than in the white matter. The first subgroup was noted corresponding to the blood flow through the white matter, and the latter through the gray matter. When the blood flow through the white and gray matter was calculated according to the subgroup data base, the flow ratio through the white and gray matter was 1:2. 4. Comparing the blood flow through each spinal cord segment revealed no significant difference at a p level of 0.01.

Animals↗