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Biomedical subjects

K Tani

Publications and source records attributed to K Tani.

At least 289 records · Page 16Linked to original sources
PubMed1989

Imported rickettsial African spotted fever in Japan.

A 45-year-old Japanese man developed a black eschar skin lesion and on the body, a number of erythematous seropapules 2 weeks after his return from South Africa. Within 24 h of treatment with minocycline he was afebrile and symptomatically much improved. Serological tests showed increased antibody titres to spotted fever group rickettsiae (SFGR). From the clinical and serological findings we consider this patient to be the first in Japan to have been infected with SFGR in Africa.

Antibodies, Bacterial↗
PubMed1989

Quantitation of autoantibody-secreting B cells in systemic lupus erythematosus.

An ELISA spot assay was used to quantitate the number of autoantibody-secreting B cells in the peripheral blood of patients with systemic lupus erythematosus. Patients with active disease had 20 fold more anti-DNA, 4 fold more anti-actin and 3 fold more anti-myosin secreting lymphocytes than controls but normal numbers of anti-cardiolipin and anti-transferrin secreting B cells. 60% of SLE patients had increased numbers of B cells reactive with multiple autoantigens. These data suggest that B cell activation in SLE may be influenced by both antigen-specific and antigen-independent factors.

Actins↗
PubMed1989

Implantation of fibroblasts transfected with human granulocyte colony-stimulating factor cDNA into mice as a model of cytokine-supplement gene therapy.

A fibroblast-mediated gene delivery method was used for the endogenous expression of human granulocyte colony-stimulating factor (G-CSF) as a model for cytokine supplement therapy. Human G-CSF cDNA was inserted into the plasmid expression vector BMGNeo, which contains a partial sequence of bovine papilloma virus and a selectable marker gene. The recombinant plasmid (BMGNeo-GCSF) was transfected into NIH/3T3 fibroblasts by the calcium phosphate coprecipitation method, and the stably transformed cells were isolated by G418 selection. An appropriate clone producing a large amount of G-CSF was selected by enzyme immunoassay of the culture supernatants. Southern blot analysis suggested that the BMGNeo-GCSF plasmid replicated mainly as an episome, and Northern blot analysis demonstrated the high expression of human G-CSF mRNA in the cells. After the implantation of the G-CSF-producing fibroblasts into nude mice, prominent neutrophilia, about 30-fold the level of normal control, was observed within seven days. Moreover, the number of hematopoietic progenitor cells in spleen remarkably increased for all cell lineages in these mice. To regulate the in vivo expression of G-CSF, we designed a subcutaneous diffusion chamber apparatus that contains the G-CSF-producing fibroblasts. The leukocytosis (neutrophilia) induced in C3H mice after embedding the device quickly disappeared after ethanol treatment of the chamber. Furthermore, reinjection of the G-CSF-producing fibroblasts into the chamber caused a second neutrophilia.

Animals↗
PubMed1989

[A case report of macroamylasemia with rheumatoid arthritis].

A patient of rheumatoid arthritis complicated with macroamylasemia. Serum amylase level was persistently elevated without any apparent cause. Amylase creatine clearance ratio was reduced inspite of normal renal function. These findings suggested the presence of macroamylasemia. The abnormal molecular size of amylase isozyme was electrophoresed. Thin layer chromatography and electroimmunosyneresis revealed that the amylase binding molecule was immunoglobulin (IgA kappa type). The etiology and the clinical significance of the macroamylasemia remain unclear. Amylase binding immunoglobulin is thought to be autoantibody to amylase. Thus, the association with autoimmune diseases is suggested. However, only few cases have been reported of autoimmune diseases complicated with macroamylasemia. In this case, macroamylase appeared about 10 months after the onset of RA. Serum amylase level was inconsistent during the clinical course. Further follow-up study is considered to be important.

Amylases↗
PubMed1989

[Pilot combination phase II study of mitomycin C plus cisplatin for non-small cell lung cancer].

Fifteen patients (six patients with adenocarcinoma, seven patients with squamous cell carcinoma, and two patients with large cell carcinoma) with advanced non-small cell lung cancer (NSCLC) were evaluable for mitomycin C (MMC; 8 mg/m2 day 1, 8, every 3-4 weeks) plus cisplatin (CDDP; 80 mg/m2 day 1, every 3-4 weeks). Ten patients had had prior chemotherapy. Among 15 evaluable patients, no patient achieved complete response, and two patients showed partial response. The response rate of MMC plus CDDP against NSCLC was 13.3%. Toxic effects included anorexia (80%), nausea and vomiting (67%), leukopenia (53%), anemia (47%), nephrotoxicity (47%), thrombopenia (27%), liver injury (27%), and fever (7%). These toxic effects were reversible and manageable. The combination of MMC and CDDP appears to be valuable regimen against advanced NSCLC.

Aged↗
PubMed1989

Therapeutic effect of aspoxicillin on experimental pneumonia with Klebsiella pneumoniae in mice.

The therapeutic effects of aspoxicillin (ASPC) on an experimental pneumonia in mice were compared with those of piperacillin (PIPC) and mezlocillin (MZPC) under various administration schedules. The pneumonia was induced with K. pneumoniae B-54 by the aerosol method. Fifty mg/kg of each penicillin was subcutaneously injected into mice starting from 12 h after infection. At 3- and 6-h interval regimens, ASPC caused the infected mice to survive longer than the other penicillins. The decrease of viable bacterial counts in the lung after a single or repeated injection of ASPC occurred more rapidly than with the other drugs. The concentration of ASPC in the lung after a single injection was higher than that of the other drugs and the concentration was maintained above the MIC for about 2 h. The therapeutic effects of these penicillins on this model reflected well their concentrations in the lung. Among these penicillins, ASPC gave the highest maximum level and persisted longest in the lung, so is shown to have a therapeutic effect superior to PIPC and MZPC on this model of pneumonia. The findings obtained in this experimental pneumonia model were concluded to correlate well with the good clinical efficacy of ASPC compared to PIPC.

Amoxicillin↗
PubMed1989

[Surgical treatment of lung cancer with chest wall invasion].

From 1955 through 1987, 64 cases underwent operation at Mie University Hospital for carcinoma of the lung invading the chest wall (p-T3). According to the classification by Mishina et al, the extent of tumor invasion of the chest wall was p3a-b in 63%, p3c in 18%, and p3d in 19%. Histologically, the tumors were epidermoid carcinoma in 565, adenocarcinoma in 31%, large cell carcinoma in 11%, and small cell carcinoma in 2%. The post surgical staging of N factor was N0 in 39%, N1 in 33%, and n2 in 28%. Extrapleural resection was performed in 27 cases and extended resection (en block resection of chest wall and lung) was performed in 30 cases with an operative mortality of 0%. The actuarial three-year survival rate (Kaplan-Meier method) for patients with p3d was 18.2% but three-year survival for patients with p3a-b and p3c was more than 30%. In spite of p3a-b, however, four-year survival for patients without extended resection was decreased to 11.6%. Four-year survival of patients with adenocarcinoma, epidermoid carcinoma, and large cell carcinoma was 5.9%, 28.4% and 75% respectively, lymphatic metastases reduced survival, with a three-year survival rate of 52.8% for patients with N0 disease and 8.35 for those with N2 disease. Among patients with extended resection, four-year survival for patients 60 years of age of less was 50.8%, greater than the 13.3% four-year survival for the patients more than 61 years of age. We conclude that long-term survival can be influenced by the extent of tumor invasion. In factor, histologic type, and the patient's age, and that extended resection and adjuvant therapy should be applied for treatment of lung cancer with chest wall invasion.

Adult↗
PubMed1989

[The effects of administration of haptoglobin for hemolysis by extracorporeal circulation].

It is well known that hemolysis by extracorporeal circulation is major cause of renal failure after open heart surgery. The purpose of this study is to investigate the effects of administration of haptoglobin (Hp) during extracorporeal circulation. The patients were divided into two groups: Group I 10 patients underwent open heart surgery for extracorporeal circulation; Group II 10 patients underwent open heart surgery for extracorporeal circulation with the administration of 4,000 IU of Hp. The serum level of total Hp was elevated in group II during bypass, and reduced at 1st day in both groups more than pre-operative level. The serum level of total Hb was elevated in both groups during bypass, and reduced at 1st day within normal limits. Free serum Hb was found in group I at 30 min after start bypass and increased during bypass and urinary Hb was also found. However, in group II free Hb was not found during and after bypass. Urinary NAG and alpha 1 Mg levels of group I were significantly higher than those of group II. The administration of Hp during extracorporeal circulation prevents the increment of serum free Hb and is effective for protection of renal function.

Acute Kidney Injury↗
PubMed1988

Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma.

Two overlapping clones, covering the entire coding sequence of human M2-type pyruvate kinase (PK) cDNA, were isolated and sequenced. Nucleotide sequencing results showed that they contained the 109-bp 5'-untranslated region, the 1593-bp coding region and the 585-bp 3'-untranslated region. Nucleotide sequence homology was 90% and 69% with rat M2-type and L-type PK cDNA, respectively. In situ hybridization using the human M2-type PK cDNA probe disclosed that the gene for M2-type PK is located at band q22 on chromosome 15. Northern blot analysis with RNA from human hepatoma demonstrated that M2-type PK was predominantly expressed in hepatoma cells, whereas L-type PK was preferentially expressed in the non-tumor portion of the liver.

Amino Acid Sequence↗
PubMed1988

Trans-activation of class II (I-A alpha) gene by I-A beta gene transfection using bovine papilloma virus as a shuttle vector system.

We have engineered a bovine papilloma virus to carry the class II MHC (A beta d) gene. The recombinant plasmid was introduced into various mouse B cell hybridomas by co-transfecting with the neomycin-resistance gene. Several transfectants which received only the beta-chain gene of I-Ad, expressed I-Ad proteins on the cell surface. Their presence was determined by direct immunofluorescence and by Northern blot hybridization as well as RNA dot blot hybridization. These I-Ad molecules could induce IL-2 production in I-Ad restricted T cell hybridomas. When I-Ad molecules are expressed on the cell surface of the transfectants, they become fully active in inducing the I-Ad restricted T cell response. The original B cell hybridomas used for transfection possessed the alpha and beta chain genes of the I-Ad molecule but there are no transcripts of these genes in the cell. We suppose that many copies of the transfected beta-chain gene or its product may also induce production of cellular alpha-chain gene products and thereby induce expression of the I-Ad molecule on the cell surface.

Animals↗
PubMed1988

Genotypic analysis using a Y-chromosome-specific probe following bone marrow transplantation.

To monitor successful engraftment after bone marrow transplantation, we performed Southern hybridization analysis or dot blot analysis of DNA in a set of sex-mismatched cases using a Y-chromosome-specific DNA probe (pHY10). This method was extremely sensitive and rapid for checking which cells contain the Y-chromosome. Using this probe, analysis of cells from peripheral blood and bone marrow after transplantation demonstrated the usefulness of confirming engraftment of donor cells and of detecting mixed lymphohematopoietic chimerism.

Adult↗
PubMed1988

Elevated erythrocyte adenosine deaminase activity in a patient with primary acquired sideroblastic anemia.

We report a case of primary acquired sideroblastic anemia (PASA) associated with elevated erythrocyte adenosine deaminase (ADA) activity. The patient was an 85-year-old Japanese male. Analysis of the peripheral blood revealed pancytopenia, and the bone marrow findings showed marked ringed sideroblasts and chromosomal deletion (46XY, 11q-). The erythrocyte ADA activity was 17 times higher than that of normal control, the leukocyte ADA activity was within the normal range, and the plasma ADA activity was 2 times higher than the normal mean. The adenine nucleotides in the patient's erythrocytes were within normal range. According to starch gel electrophoresis, ADA isozyme of the patient was ADA 1. Western blotting showed an increased amount of ADA protein in the patient's erythrocytes. Southern blotting revealed no gene amplification or large structural change. Dot blot analysis of the reticulocyte mRNA showed no increase in the amount of ADA mRNA in the patient's reticulocytes compared with those of reticulocyte-rich controls. We considered that the mechanism of elevated ADA activity in this acquired defect was similar to that found in hereditary hemolytic anemia associated with ADA overproduction.

Adenine Nucleotides↗
PubMed1988

Genetic analysis of adenosine deaminase expression in adult T-cell leukemia.

We investigated the genetic basis for the increased adenosine deaminase (ADA) expression in adult T-cell leukemia (ATL). We found a correlation between the levels of ADA-specific mRNA and ADA immunoreactive protein in ATL. Southern blot analysis revealed no gene amplification or rearrangement of the ADA gene. These findings indicate that increased ADA expression in ATL cells reflects increased transcriptional activity for the ADA gene or increased stability of ADA mRNA.

Acute Disease↗
PubMed1988

Two homozygous cases of erythrocyte pyruvate kinase (PK) deficiency in Japan: PK Sendai and PK Shinshu.

Two new erythrocyte pyruvate kinase (PK) variants with severe nonspherocytic hemolytic anemia are presented. These cases are both considered to be homozygous because of the consanguineous marriages in their parents. Their erythrocyte PK's were characterized by the recommended methods of the International Committee for Standardization in Haematology (ICSH). These two variants have been named PK Sendai and PK Shinshu. PK Sendai showed a high K0.5S (phosphoenolpyruvate), was remarkably inhibited by ATP, and was thermolabile, while PK Shinshu demonstrated remarkably low enzyme activity and required a high level of fructose 1,6-diphosphate for activation.

Adolescent↗