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Biomedical subjects

K Takeuchi

Publications and source records attributed to K Takeuchi.

At least 631 records · Page 35Linked to original sources
PubMed1995

An adult diagnosed as hyper-IgM immunodeficiency syndrome.

A 27-year-old male who visited our hospital because of pneumonia was diagnosed as hyper-IgM immunodeficiency syndrome. His serum IgM level was markedly elevated, while the serum level of IgD was normal with a markedly decreased level of serum IgG and IgA. The proportion of T and B cells of peripheral blood lymphocytes was normal. However, B cells bearing surface IgG or IgA were not detectable by immunofluorescence technique. There was a consanguineous marriage in his family, suggesting that his disorder was caused by a genetic abnormality such as X-linked recessive and also autosomal recessive inheritance, although further study is necessary. CD40 ligand cDNA did not appear to contain any abnormal changes within the coding region.

Adult↗
PubMed1995

Arteriovenous malformation associated with a large cyst--case report.

A 37-year-old male presented with an arteriovenous malformation in the left temporal lobe associated with a large cyst. The cyst was drained and the nidus completely excised. The histological findings suggest that repeated subclinical hemorrhages from neovascular channels of the cyst membrane was responsible for the growth of the cyst.

Adult↗
PubMed1995

[A case of incarceration of a right femoral hernia in a very old female with a intriguing clinical course].

The patient was a 94-year-old woman. She was admitted on our hospital for ileus due to incarceration of a right femoral hernia on October 29, 1993. Although an emergency operation was necessary she refused, therefore we continued to have conservative therapy. After repeated persuasion, she agreed the operation which was performed under spinal anesthesia on November 17. The peritoneal cavity was opened through a transverse incision of the lower abdomen. Some bloody ascites was encountered. Part of the jejunum was incarcerated through right femoral canal and was completely obstructed, with ischemic but no necrotic changes. It was excised. She resumed oral intake on the 7th postoperative day and was discharged on the 77th postoperative day. Initially due to her opposition we could not perform the operation, but after persistent persuasion she agreed and survived. Good communications between the doctor and the patient, and of course informed consent, was very important in such cases.

Aged↗
PubMed1995

Establishment of a new human cell line, LI90, exhibiting characteristics of hepatic Ito (fat-storing) cells.

BACKGROUND: Thus far, human hepatic Ito (fat-storing) cell lines have not been established. Therefore, functional characteristics of Ito cells have not been fully investigated. EXPERIMENTAL DESIGN: We established a new cell line, LI90, that exhibited characteristics compatible with those of Ito cells from a human hepatic mesenchymal tumor. LI90 cells were examined with phase-contrast microscopy, immunohistochemistry, and cytogenetics, and their vitamin A-storing activity was analyzed. To obtain a marker specific for Ito cells for immunohistochemical analyses, we raised mAb against LI90 cells and clarified the molecular nature of the Ag recognized with the new Ab using an expression cloning approach. RESULTS: LI90 cells showed polygonal shape and had well developed alpha-smooth muscle actin filaments in their cytoplasm. In an overconfluent culture condition, LI90 cells aggregated to form a typical hills-and-valleys structure, LI90 cells produced various connective tissue components, such as collagen types I, III, IV, V, and VI, laminin, and fibronectin. In culture media containing vitamin A, LI90 cells formed many fat droplets in their cytoplasm, and fluorescence characteristic of vitamin A was observed in the droplets. By immunizing mice with LI90 cells, three separate mAb specifically reacting with Ito cells in human liver sections were established, and the Ag recognized with all three Ab were identified as extracellular matrix tenascin. CONCLUSIONS: The above-described morphologic and functional characteristics, including vitamin A-storage and biosynthesis of tenascin, are compatible with those of Ito cells. Therefore, LI90 cells will be useful for in vitro studies of functions of human Ito cells.

Antibodies, Monoclonal↗
PubMed1995

In vivo angioscopic detection of the damaged endothelium on the atheromatous plaque: application with vital staining by methylene blue.

We attempted to detect the lesion of the damaged endothelium on the atherosclerotic plaque in vivo with dye and angioscope. The plaques were induced on the aortae of rabbits by the continuous mechanical injury with the insertion of the polyethylene tube and feeding with 2% cholesterol-added diet. We observed the some plaques could be stained with 5 x 10(-2) mol/L methylene blue by angioscope. Control aorta, without any stimulations on the aorta, could not be stained. One month after injury and cholesterol feeding the plaques were stained. In such plaques, the lack of the endothelium and the existence of the lipid-containing cell in the intima was observed. However, the plaques of the aorta 6 months after finishing these procedures could not be stained. In these plaques, the morphological characteristics known as the regression of the atheromatous plaque, ie, the regeneration of the endothelium, the thick collagen layer on the plaque and the massive calcification of the plaque were observed. The results obtained were as follows. (1) The atheromatous lesion with the damaged endothelium could be detected in vivo by our vital staining method. (2) The atheromatous plaques stained in vivo are the lesion with complication and enhanced permeability. (3) The slightly stained plaques indicate the lesions with regression and lost high permeability. (4) Our method would be able to add important information on the atheroscrelotic lesions in the clinical situations than that now we perform.

Angioscopy↗
PubMed1995

[Fructose 1,6-diphosphate can be a substrate to accelerate glycolysis].

Fructose 1,6-diphosphate (F1, 6P) is a glycolytic intermediate which has already been used clinically to treat congestive heart failure. F1, 6P has been shown experimentally to improve glycolytic flux, although theoretical background is unclear. Since there is two possible mechanism of F1, 6P effect on acceleration of glycolysis, including pharmacological effect and substrate effect, we sought to determine the real mechanism of action of F1, 6P on glycolysis. Langendorff-perfused rabbit hearts were infused with F1, 6P (5 and 10 mM) in a first group, and in a second group, 30 minutes of perfusion with modified Krebs-Henselit (K-H) buffer with reduced glucose concentration (5 mM), plus 2-deoxy glucose (5 mM) and with or without 10 mM F1, 6P followed by 30 minutes of wash with normal K-H buffer. We measured contractile function, oxygen consumption, and high energy phosphate by 31p-NMR spectroscopy. In the first group of experiments, F1, 6P resulted in a dose dependent increase in high energy phosphate production and inorganic phosphate (Pi). There was also a marked decline in developed pressure (Dev P) due mostly to the ability of F1, 6P to chelate calcium. Intracellular Mg2+ which was also reduced during F1, 6P infusion might be related to intracellular Ca2+ and/or accelerated glycolysis. In the model of glycolysis inhibited by 2-DG, Dev P was consistently decreased during the infusion of 2-DG, and wash-out period along with the deterioration of high energy phosphate. However, F1, 6P could provide excellent recovery of contractile function after wash along with superior high energy potential during the 2-DG infusion and wash.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1995

[Improved protection of hypertrophied myocardium in ischemia by histidine buffer cardioplegia].

Pressure overload hypertrophy has been known risk factor for open heart surgery, because of accelerated loss of high energy nucleotides, greater accumulation of H+ and lactate, and earlier onset of contracture. To test the efficacy of histidine containing cardioplegia (HB) on promotion of anaerobic glycolysis leads to improved preservation of hypertrophied heart, hypertrophied rabbit heart (aortic banding at 10 days) received HB or high potassium Krebs buffer solution (KCl) were subjected to 40 min of 37 degrees C ischemia followed by reperfusion in an isolated Langendorff mode. There was significantly higher phosphocreatine, intracellular pH and lactate production which was an indicator of anaerobic glycolysis and better recovery of cardiac function in HB group compared to KCl group. We concluded that histidine containing cardioplegia was able to promote anaerobic glycolysis to maintain high energy phosphates as well as contractile function in hypertrophied myocardium.

Animals↗
PubMed1995

An angiotensin II receptor antagonist attenuates left ventricular dilatation after myocardial infarction in the hypertensive rat.

OBJECTIVE: The aims were (1) to investigate the effect of hypertention on left ventricular dilatation and haemodynamic alterations following acute myocardial infarction in spontaneously hypertensive rats (SHR) and normotensive rats (WKY); (2) to compare haemodynamic indices between the two groups; (3) to assess whether the angiotensin II type 1 receptor antagonist (AIIA), TCV-116, prevented left ventricular dilatation after myocardial infarction; and (4) to compare the effect of AIIA with that of the angiotensin converting enzyme (ACE) inhibitor, delapril. METHODS: Myocardial infarction was produced in SHR and WKY by coronary artery ligation. Haemodynamic measurements were obtained three weeks later in rats that had been treated from the next day after the operation for three weeks with TCV-116 (1 mg.kg-1.d-1) or delapril (1 g.litre-1 in drinking water), and in untreated controls. RESULTS: After myocardial infarction, left ventricular weight, and left ventricular weight were greater in SHR than in normotensive rats. Right ventricular weight, left ventricular end diastolic pressure, and LVEDVI correlated positively with infarct size in both SHR and WKY and these slopes were steeper in SHR than in WKY (P < 0.05). TCV-116 and delapril each significantly attenuated the increases in left ventricular end diastolic pressure, left ventricular weight, right ventricular weight, and LVEDVI following myocardial infarction in both in WKY and SHR, and shifted pressure-volume curve significantly to the left. CONCLUSIONS: Hypertension accelerates left ventricular dilatation and haemodynamic alterations following myocardial infarction in rats. These effects are attenuated by an angiotensin II type 1 receptor antagonist as well as by an ACE inhibitor.

Angiotensin-Converting Enzyme Inhibitors↗
PubMed1995

Determination of the spleen-blood partition coefficient for water with oxygen-15-water and oxygen-15-carbon dioxide dynamic PET steady-state methods.

METHODS: Regional splenic blood flow (SBF) was quantified by PET using a steady-state method with 15O-carbon dioxide. SBFs were determined using 104 tomographic planes obtained from 49 patients. RESULTS: When the spleen-blood partition coefficient for water (p) was > or = 0.85, significant correlations (p < 0.005) were found between SBF values determined by the steady-state and dynamic methods. The best correlation between SBFs determined by the two methods (r = 0.571) was found when p = 0.93. The best regression line, however, was thought to be the line when p = 0.93. The regression line between SBF calculated by the steady-state method (y) and SBF determined by the dynamic method (x) was y = 0.57 x + 0.03 with an F ratio of 48.75 (d.f. = 103, p = 5.0 x 10(-8)%, by ANOVA) when p = 0.92. CONCLUSION: A quick evaluation of SBF can be made by using the newly defined regression line.

Carbon Dioxide↗
PubMed1995

[The development of a tissue calcium-detecting apparatus].

Calcium phosphate is deposited in tissues with progression of aging and arteriosclerosis, causing impairments of organ functions. Localization and quantification of tissue calcium phosphate are important for elucidation of the cause of this calcium phosphate deposition. Tissue calcium phosphate has been detected by Von Kossa-Nishiyama staining using the heavy metal substitution technique. By this method, however, the reaction was weak because of the use of sunbeam as the light source, and consistent detection was difficult. Especially, fine calcium phosphate granules that appear in early stages of the pathological changes could not be detected. Therefore, we evaluated a method for preparation of tissue specimens, light source, filter, and staining conditions, and developed a more reliable method for detection and assay of tissue calcium phosphate. The human aortic tissue was fixed with formalin, embedded with paraffin, and cut to a thickness of 4 microns using a glass knife. Solax-XCA100B, an artificial sunbeam lamp with a spectrum nearly identical with that of the sun, was used as the light source. A B filter, which selectively filters out the wavelengths 290nm or less and 800-1,000nm, was used. The appearance of abnormal granules due to excessive reaction could be prevented by eliminating ultraviolet rays with wavelengths of 290nm or less, and burning of the tissue and the appearance of silver particles could be prevented by excluding infrared rays with wavelengths of 800nm or above. Distilled water was continuously dripped into the reaction solution to prevent an increase in the concentration due to evaporation. The reaction showed appreciable progression until after 40 minutes and stopped completely after 80 minutes.(ABSTRACT TRUNCATED AT 250 WORDS)

Aorta↗
PubMed1995

[Stereotactic radiosurgery with the gamma knife for brain metastases in patients with lung cancer].

Between February 1992 and April 1993, six patients with lung cancer were treated with gamma knife radiosurgery for brain metastases. Five patients had adenocarcinoma, and one patient had small cell carcinoma. Two patients had solitary metastases, and four patients had multiple metastases. Twelve metastases were treated with the gamma knife (peripheral dose between 12 Gy and 25 Gy). After radiosurgery, three complete and eight partial responses were achieved, which resulted in an overall response rate of 92%. In two patients, histological studies showed that few viable cells were surrounded by necrosis. Neurological status improved in all patients, and none died of complications. However, four of six patients later developed new intracranial metastases outside the treatment field. These data suggest that radiosurgery with the gamma knife is effective against brain metastases in patients with lung cancer, especially when the lesions are deep in the brain.

Adenocarcinoma↗
PubMed1995

Twenty-four hour monitoring of pH in the gastric tube replacing the resected esophagus.

BACKGROUND: Peptic ulcers in the gastric tube replacing the resected esophagus develop silently and cause serious problems. In this study, the acidity of the gastric tube was examined by 24-hour pH monitoring to determine if the acidity of the gastric tube was sufficient to cause peptic ulcers. STUDY DESIGN: The acidity of a gastric tube was evaluated by 24-hour pH monitoring of both the fundus and the antrum in 55 patients treated for carcinoma of the esophagus. The correlation between the fasting serum gastrin concentration and the intraluminal acidity of the completely vagotomized gastric tube was examined. RESULTS: In the patients with high postoperative acidity in the fundus or the antrum (46 percent of the 41 patients examined), the intraluminal pH remained consistently low, even long after operative treatment. Significant correlations existed between the percentage of time that the pH remained below 3 preoperatively and postoperatively in both the fundus and the antrum (r = 0.4777, p = 0.0386, and r = 0.7597, p = 0.0002, respectively). The percentage did not decrease significantly postoperatively. A significantly negative correlation (r = -0.783401, p < 0.0001) was found between the fasting serum gastrin level and the proportion of time that the nocturnal pH in the antrum remained below 3. CONCLUSIONS: Even long after esophagectomy, the pH of the gastric tube is low enough to cause peptic ulcers, especially in patients with high preoperative acidity. In these patients, the intraluminal pH in the antrum of the gastric tube correlates inversely with the fasting serum gastrin level.

Aged↗
PubMed1995

[Reticulated platelets--automated measurement and clinical utility].

Development of the method to determine reticulated platelets is briefly reviewed. A new rapid method to automatically count reticulated platelets is very recently established by our research group. The principle of the measurement of reticulated platelets is based on flow cytometry. The platelets are quickly stained with a RNA fluorescent dye, auramine O and fluorescent intensity (RNA content) and forward scatter (cell size) are measured in only 80 seconds with a reticulocyte counter, equipped with special software for analysis of reticulated platelets. Both of the reproducibility and the linearity were shown to be good. Normal percentage value for reticulated platelets was 0.98% +/- 0.41% and its absolute count was 2.12 +/- 0.69 x 10(9)/l. The absolute count was decreased in patients with reduced thrombopoiesis such as acute myeloblastic leukemia, aplastic anemia and was elevated in patients with essential thrombocythemia and in chronic myelocytic leukemia. The patients with chronic idiopathic thrombocytopenic purpura had a high percentage of reticulated platelets. An apparent rise was noticed in the percentage of reticulated platelets which preceded by several days a progressive increase in the platelet count at the recovery phase of thrombocytopenia in a couple of leukemic patients. These suggest that an automated measurement of reticulated platelets can be clinically useful to estimate thrombopoiesis in bone marrow.

Adolescent↗
PubMed1995

Characterization of the facilitatory prejunctional purinoceptor on adrenergic nerves of the rabbit ear artery.

The effects of a number of purinoceptor agonists and antagonists on the release of endogenous norepinephrine (NE) from electrically field-stimulated (2 ms, 4 Hz) rabbit ear arteries were determined. NE was quantified by high-performance liquid chromatography and electrochemical detection techniques. Both P1- and P2-purinoceptor agonists enhanced the release of NE in a concentration-dependent manner (0.1 microM-100 microM), the relative order of potency being beta, gamma-methylene ATP (beta gamma-mATP) > 2-chloroadenosine (2CA) > ATP > L-phenylisopropyl-adenosine > 2-methylthio ATP > 5'N-ethylcarboxamido-adenosine. Adenosine and alpha, beta-methylene ATP (alpha beta-mATP) at 100 microM did not significantly enhance the electrical field stimulation-evoked release of NE. None of drugs changed the spontaneous overflow of NE. Pretreatment with alpha beta-mATP (1, 10 microM), a P2x-purinoceptor desensitizing agent, and 8-sulfophenyl theophylline (8SPT; 1 and 10 microM), a P1-purinoceptor antagonist, caused a shift to right of the concentration-release curve for beta gamma-mATP. alpha beta-mATP and 8SPT did not by themselves significantly affect the spontaneous overflow and the electrical field stimulation-evoked release of NE. Pretreatment of alpha beta-mATP (30 microM) and 8SPT (30 microM) antagonized facilitation of NE release by 2CA (10 microM). The concentration-contraction curve for beta gamma-mATP (1-300 microM) in the rabbit ear artery was shifted to the right by alpha beta-mATP (30 microM) but not by 8SPT (30 microM).(ABSTRACT TRUNCATED AT 250 WORDS)

2-Chloroadenosine↗
PubMed1995

Nitric oxide and prostaglandins in regulation of acid secretory response in rat stomach following injury.

The gastric mucosa responds to taurocholate (TC) by significantly decreasing acid secretion. We examined the role of nitric oxide (NO) in this phenomenon in comparison with endogenous prostaglandins. A rat stomach was mounted in an ex-vivo chamber and perfused with saline, and the potential difference, luminal pH and acid responses were measured before and after the application of 20 mM TC for 30 min with or without pretreatment with the NO synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) or the cyclooxygenase inhibitor indomethacin. Exposure of the stomach to TC caused a reduction in potential difference, a decrease in acid secretion and an increase in luminal HCO3-. Pretreatment with L-NAME or indomethacin did not affect potential difference and HCO3- responses, but it significantly attenuated the decrease in acid secretion caused by TC. The effect of L-NAME was more potent than that of indomethacin, and, especially in the presence of L-NAME, acid secretion was actually enhanced after exposure to TC. Aminoguanidine, the selective inhibitor of inducible NO synthase, did not have any significant effect on either parameter. This effect of L-NAME was antagonized by the simultaneous administration of L-arginine but not by that of D-arginine, whereas the effect of indomethacin was reversed by PGE2. Acid secretion in normal stomachs was significantly reduced by nitroprusside and PGE2 but was not affected by either L-NAME or indomethacin.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1995

[Electromyographic findings in muscles around the osteoarthritic knee: integrated electromyography and frequency analysis].

An evaluation of the muscular functions of the vastus medialis, vastus lateralis, rectus femoris, medial hamstring, and of the lateral hamstring was performed using electromyography in 33 knees of 26 female patients with osteoarthritis (OA group) and in 25 knees of 19 healthy female volunteers (control group). During standing on both feet, all muscles in the OA group showed higher IEMG (integrated electromyography) and higher LMR (IEMG of vastus lateralis/IEMG of vastus medialis ratio in the quadriceps; and lateral hamstring/medial hamstring ratio in the hamstrings) than the control group. These augmentary muscular activities ameliorated the varus deformity caused by the osteoarthritis. During maximum isometric voluntary contraction, the OA group showed lower extension and flexion torque of the knees and also lower IEMG than to the control group, while the IEMG of vastus lateralis was not lower. These findings indicated decreased muscular activities in the osteoarthritic knee, and that the activity of the quadriceps was maintained mainly by the vastus lateralis under such conditions. Frequency analysis of the myoelectric signal during maximum isometric voluntary contraction revealed a single peak of low frequency in the power spectrum density function of the quadriceps and double peaks of low and of high frequency in the hamstring. In the OA group, the peak height of the low frequency component was increased in the quadriceps and decreased in the hamstring. We concluded that the duration of the motor unit action potentials was affected in the osteoarthritic knee.

Aged↗