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Biomedical subjects

K Takeda

Publications and source records attributed to K Takeda.

At least 973 records · Page 54Linked to original sources
PubMed1990

[A case of long thoracic nerve palsy, with winged scapula, as a result of prolonged exertion on practicing archery].

Reports of isolated long thoracic nerve palsy are rare in Japan. We reported a case of isolated long thoracic nerve palsy, resulted from recurrent injury to the nerve. Muscle CT and electrodiagnostic study were useful for confirming diagnosis of this cases. This patient was a student aged 20 years, with nothing of importance in his family or past history. After he started practicing archery, winging of left scapula was gradually developed. Physical examination revealed weakness and atrophy of left serratus anterior muscle. There was no wasting and weakness of other should girdle muscles. Hematochemical tests were normal, except slight hyperthyroidism. Radiography and myelography of the cervical spine were normal. Muscle CT of upper thoracic level demonstrated atrophy of left serratus anterior muscle, and no abnormality were found in other muscles. Electromyogram of the left serratus anterior revealed discrete activity of reduced amplitude, and fibrillation potentials and positive sharp waves. Conduction time for left long thoracic nerve was prolonged, and amplitude of the evoked response was small and there were temporal dispersion. Muscle CT and electrodiagnostic studies were suggestive of neuroapraxia of left long thoracic nerve. Over stretching or compression during exercises may be responsible for the damage to the long thoracic nerve.

Adult↗
PubMed1990

Relationship between asthma and seasonal allergic rhinitis in schoolchildren.

The relationship between sensitization to common allergens and the development of asthma and seasonal allergic rhinitis (SAR) was investigated in a total of 419 schoolchildren in a farming community where allergens of both Dermatophagoides farinae (Df) and orchard grass (OG) pollen predominate. Twelve subjects (2.9%) were found to have asthma and 35 subjects (8.4%) SAR. There was no significant association between occurrences of asthma and SAR. The incidence of asthma correlated with DF-specific IgE levels but not with OG-specific IgE levels, whereas that of SAR correlated with OG-specific IgE levels but not with Df-specific IgE levels. No significant correlation was detected between IgE antibody levels against Df and OG. These results suggest that asthma occurs independently of SAR.

Adolescent↗
PubMed1990

[Computed tomographic findings of peripheral-type schwannoma; comparative study with histology].

Computed Tomographic (CT) findings of peripheral-type schwannomas were reviewed and compared with the histology. Ten patients were examined. Plain CT showed all tumors as hypodense mass. After injection of contrast material, they were enhanced in various fashions. The enhancement patterns were divided into four types. On enhancement CT, highly enhanced area in the tumor was consistent with hypercellular region or vascular rich area, whereas poorly stained area revealed hypocellular area, interstitial xanthomatous change or cystic degeneration. It is concluded that the inter-tumoral difference in special distribution of hyper- and hypocellular areas leads to the variety of enhancement pattern on CT images.

Adult↗
PubMed1990

[Dural arteriovenous malformation of the spine].

A case of dural arteriovenous malformation of the spine was reported. The patient was admitted to our hospital because of paraparesis, and selective angiography revealed dural AVM of spine. Myelo-CT and MR imaging showed abnormal vessels in the spinal canal. After embolization, dural AVM was disappeared.

Aged↗
PubMed1990

[Urothelial tumor in dialysis patients; report of four cases].

Four cases of urothelial tumors diagnosed during hemodialysis were reported. Patients' ages ranged from 43 to 74 years old. One case was female and others were male. Durations of dialysis at the time of diagnosis were 13 to 24 months. Chief complaints of these four cases were gross hematuria. One case with bladder tumor underwent emergency operation because of repeated bladder tamponade. Partial cystectomy was performed on this case. Other two cases with bladder tumor were treated with endoscopic method. Simple nephrectomy was done in the case with renal pelvic tumor. Post operative course in all four cases were uneventful without major complications.

Adult↗
PubMed1990

[Assessment of the biological activity of residual or recurrent neuroblastoma tumors with 131I-metaiodobenzylguanidine (MIBG) scintigraphy].

131I-Metaiodobenzylguanidine (MIBG) is now accepted as a useful agent for the diagnosis of adrenal medullary tumor. The aim of this paper is to evaluate 131I-MIBG for the assessment of the biological activity of residual or recurrent tumor after initial treatment in patients with neuroblastoma. Nineteen scans were performed for 9 patients, mean age 3.5 years-old. Computed tomography demonstrated paravertebral mass in six and metastatic liver tumor in four cases. Anterior and posterior images of the thorax and abdomen were taken 48-72 hours after injection of 7.4-18.5 MBq (0.2-0.5 mCi) 131I-MIBG. Positive images were obtained in 8 scans for four patients and followed by rapid growth of tumor and increased urinary dopamine. The biological activity of residual or recurrent tumor was thought to be high in these patients. Eleven scans for 5 patients revealed negative. In four of them, the tumor size reduced and urinary dopamine value remained within normal limits on the follow-up study. The tumor was assumed to have low biological activity in these patients. One case in which initial scan was negative became positive on the follow-up study. 131I-MIBG activity did not well correlate with urinary vanillylmandelic acid as compared with urinary dopamine. In conclusion, 131I-MIBG proved to be useful for assessing biological activity of residual or recurrent tumor of neuroblastoma and estimating the prognosis of the patient.

3-Iodobenzylguanidine↗
PubMed1990

Mechanism of contractile response to bombesin of the longitudinal muscle from guinea pig ileum.

The mechanism of action of bombesin in the longitudinal muscle with myenteric plexus (LM-MP) preparation from guinea pig ileum was examined. Bombesin (3 x 10(-10) to 3 X 10(-7) M) induced contraction in a concentration-dependent manner. The contractions induced by bombesin at concentrations of 10(-9) M and less were tetrodotoxin- and scopolamine-sensitive, and those induced at concentrations of 10(-8) M and greater were tetrodotoxin-sensitive, and partially scopolamine-sensitive. In association with the mechanical response to bombesin, the peptide induced Ca(++)-dependent and tetrodotoxin-sensitive release of acetylcholine from the LM-MP preparation. Bicuculine inhibited partly both the cholinergic contraction and the release of acetylcholine induced by bombesin, suggesting the involvement of a gamma-aminobutyric acid (GABA)-ergic mechanism. Bombesin also induced Ca(++)-dependent and tetrodotoxin-sensitive release of endogenous and [3H]GABA from the LM-MP preparation. These results indicate that bombesin induces contraction mainly mediated mediated by cholinergic and partially mediated by noncholinergic excitatory neurons. GABAergic neurons may be involved in a part of the response to bombesin mediated by the cholinergic neuron.

Acetylcholine↗
PubMed1990

[A study of bleeding peptic ulcer in the elderly in the view of risk factors].

We treated 448 patients for peptic ulcer from 1970 to 1988. Among them, 52 cases were bleeding peptic ulcer in the elderly. 32 cases (61.6%) had a duration of illness of less than 3 months, and the most common symptom was abdominal pain (51.9%). 22 cases (44.0%) were in shock and 32 cases (62.7%) had accessory diseases. Twelve cases were treated medically, while 40 cases were treated surgically. Elective operations were performed in 15 cases, and their mortality was 6.7%. Emergent operations were performed in 25 cases, and their mortality was 28.0%. The mortality of of cases involving both shock and accessory diseases was 57.1%, and of this group, all 3 cases treated medically died. When bleeding exceeded 2000cc was the mortality 46.7%. Regarding cases of emergent operation involving shock or accessory diseases, the mortality was 18.2% in the cases treated medically for less than 4 days and had the episodes of massive bleeding less than 3 times, but 62.5% in other cases. The results of this study suggest that aggressive surgical treatment in an early stage may reduce the mortality in elderly patients with peptic ulcer.

Aged↗
PubMed1990

In vivo evidence for carrier-mediated uptake of beta-lactam antibiotics through organic anion transport systems in rat kidney and liver.

The transport mechanisms of beta-lactam antibiotics in the rat kidney and liver were studied with an in vivo tissue-sampling single-injection technique using [3H]benzylpenicillin [( 3H]PCG) as a substrate. Concentration-dependent uptake of [3H]PCG was observed in the kidney, and the in vivo kinetic parameters were estimated as follows: the maximum uptake rate (Jmax) was 6.88 mumol/min/g of kidney, MIchaelis constant (Kt) was 1.39 mM and nonsaturable first-order rate constant (kd) was 0.414 ml/min/g of kidney. The uptake of [3H]PCG was inhibited by organic anions but not by organic cations. Several beta-lactam antibiotics also reduced the uptake of [3H]PCG. Furthermore, the organic anion, probenecid, and beta-lactam antibiotic, cefpiramide, showed a dose-dependent inhibitory effect. These results suggest participation of an organic anion transport system in uptake of beta-lactam antibiotics across the renal plasma membrane. Saturable uptake of [3H]PCG was also observed in the liver and Jmax, Kt and Kd were estimated to be 3.62 mumol/min/g of liver, 3.59 mM and 0.223 ml/min/g of liver, respectively. The in vivo influx rate calculated from Jmax/Kt in the liver was 1.01 ml/min/g of liver and was close to the in vitro value, 1.54 ml/min/g of liver, estimated previously from isolated hepatocytes. Although dipeptides and organic cations showed no effect on the hepatic uptake of [3H]PCG, probenecid significantly reduced its uptake. Several beta-lactam antibiotics also reduced the uptake of [3H]PCG by the liver. These features of the hepatic uptake of beta-lactam antibiotics through an organic anion transport system are in agreement with the previous results obtained in isolated hepatocytes.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1990

[Investigation of adsorption, metabolism and excretion of cefuroxime axetil in volunteers of gastrectomized patients].

Adsorption, metabolism and excretion (ADME) of cefuroxime axetil (CXM-AX) 500 mg given orally at 30 minutes after meals to male volunteers who had undergone gastrectomy was compared with that of healthy volunteers. 1. The transition of the drug's serum concentrations was observed as follows: Tmax was somewhat shorter and T 1/2 longer in the gastrectomized volunteer group than in the healthy volunteer group. 2. Total urinary recovery rates of cefuroxime (CXM) were not significantly different between the 2 groups, averaging 53.6% for the gastrectomized volunteer group and averaging 54.5% for the healthy volunteer group. 3. Total urinary recovery rates of CXM-delta 2 were not significantly different between the 2 groups either, averaging 1.7% for the gastrectomized volunteer group and 1.3% for the healthy volunteer group. 4. The above results suggest that ADME of CXM-AX in the gastrectomized volunteers is not fundamentally different from that of the healthy volunteers. The absorption, however, may be reduced when peristalsis of the intestinal tract is accelerated as a result of gastrectomy.

Administration, Oral↗
PubMed1990

[Difference between the hair follicle cell and epidermal keratinocyte in vitro].

We cultured human hair follicle cells (HFC) and epidermal keratinocytes (EK) using mixed collagen membranes as the substrate for the culture, and studied the degree of differentiation of these cells. Morphologically, polygonal epithelioid arrangements were seen in both cultures. However, in the culture of EK compared with HFC, large denucleated cells appeared within shorter periods of culture and stratification of cells were more prominent. In the culture of HFC, keratohyalin granules were not observed and cornified envelope formation was suppressed compared with that in EK. Then it is suggested that HFC and EK are different in the degree of keratinization and of other nature.

Adult↗
PubMed1990

[The effect of 1, 25-dihydroxyvitamin D3 on cultured human hair follicle cells from a patients with vitamin D-dependent rickets type II with alopecia].

We examined the effects of 1, 25-dihydroxyvitamin D3 on the growth and differentiation of cultured human hair follicle cells from a patient with vitamin D-dependent rickets type II [DDR-II] with alopecia. In the presence of 100 nM 1, 25-dihydroxyvitamin D3, the clonal growth of normal hair follicle cells was inhibited and terminal differentiation was markedly stimulated. However, the cells from a patient with DDR-II with alopecia did not respond to the hormone, suggesting a lack of the specific receptors in the cells. The difference in the cellular response to the hormone between the normal hair follicle cells and those from a patient were clear and easily distinguished, and these studies may provide a basis for the rapid diagnosis of DDR-II. Large number of hairs were difficult to obtain from patients with alopecia, and we developed a new culture method to accomplish these studies. Our system may be useful in the culture of HFC from only a few hair follicles.

Alopecia↗
PubMed1989

Effects of acromelic acid A on the binding of [3H]glutamic acid and [3H]kainic acid to synaptic membranes and on the depolarization at the frog spinal cord.

Triton treatment of synaptic membranes from the frog spinal cord enhanced the specific binding of [3H]glutamic acid compared with non-treated fresh and frozen ones, but not that of [3H]kainic acid. Acromelic acid A specifically inhibited the binding of [3H]kainic acid, and was approximately 100 times more potent than kainic acid. Acromelic acid A and excitatory amino acids caused a depolarization in the ventral root of the frog spinal cord in a dose-dependent manner, and the effect of acromelic acid A was much superior to that of kainic acid or domoic acid. Acromelic acid A is one of the most potent kainic acid agonist at the frog spinal cord.

Animals↗
PubMed1989

Induction of differentiation of human monoblastic and myeloblastic leukemia cell lines by TNF muteins.

The differentiation inducing activity of five recombinant human tumor necrosis factor (rHuTNF) muteins, prepared by protein engineering techniques, was compared by measuring the ability to reduce nitroblue tetrazolium dye of human myelogenous leukemia cells. TNF(C-Phe), in which the C-terminal leucine of TNF molecule was replaced by phenylalanine, was 20-times as potent in induction of differentiation of U-937 cells as the parent TNF(N-Met), in which methionine was added to N-terminal sequence of TNF. The differentiation inducing activity of TNF muteins was not always proportional to their binding activity to the receptors nor to their cytotoxicity on U-937 cells.

Amino Acid Sequence↗
PubMed1989

[Measurement of effective hepatic blood flow using 99mTc-PMT SPECT and single blood sampling].

Effective hepatic blood flow (EHBF) was measured from an uptake constant using single blood sampling and 99mTc-PMT hepatobiliary SPECT data. After intravenous injection of 3 mCi (111 MBq) of 99mTc-PMT, serial 1 min SPECT data were obtained for 7 minutes. A time activity curve (TAC) over the heart, that was normalized with the 5 minutes venous sample concentration (%/dose/ml), was used as a blood clearance curve (B(t]. And a TAC of the whole liver, that was normalized with the injected dose of 99mTc-PMT (%/dose), was used as a hepatogram (L(t]. An uptake constant representing EHBF, was estimated from the Rutland's method L. (t)/B(t) was plotted against integral of otB(t)dt/B(t), and the slope of the least square fitted straight line was determined as the uptake constant. In 16 cases, significant correlation was obtained between the 99mTc-PMT hepatic uptake at 5 minutes and the EHBF estimated from the blood clearance (r = 0.85, p less than 0.001). While there was a much better correlation between the EHBF from uptake constant and the EHBF from blood clearance (r = 0.95, p less than 0.001). In conclusion, this analysis for uptake constant of 99mTc-PMT SPECT data enables us to estimate EHBF with single venous sampling and in relatively short acquisition time. This method is thought to be very valuable in clinical practice.

Humans↗