Cellulose acetate membrane electrophoresis of bovine serum proteins using a barbital calcium buffer.
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Acetates↗
Biomedical subjects
K Takase
Publications and source records attributed to K Takase.
[Syntheses of 1,3,4-thiadiazole derivatives].
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Azoles↗
The synthesis and metabolism of 2,3-dihydro-L-tryptophan and 2,3-dihydro-5-hydroxy-DL-tryptophan.
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5-Hydroxytryptophan↗
Intracerebral hemorrhage after transcatheter thrombolysis of non-occluding superior mesenteric artery thrombosis.
We performed transcatheter thrombolysis on a 64-year-old man with non-occluding superior mesenteric artery (SMA) thrombosis because his severe symptoms could not be controlled with medication. An enhanced computed tomography (CT) scan revealed intramural thrombosis in the SMA. We were concerned that the narrowing of the SMA lumen might progress to complete occlusion, resulting in a high likelihood of mortality. After dissolution of the SMA thrombosis, the original symptoms almost completely disappeared. However, intracranial hemorrhage occurred 8 hr after thrombolysis, requiring surgical intervention. Transcatheter thrombolysis is thought to be a useful treatment for SMA thrombosis, especially in elderly patients with a high operative risk; however, the possibility of intracerebral hemorrhage must be taken into consideration.
Angiography↗
Prognostic value of peritoneoscopic findings in cirrhosis of the liver.
The prognostic value of peritoneoscopy was examined in 372 patients with liver cirrhosis according to the degree of development and size of regenerating nodules, the development of reddish markings, the development of small lymphatic vesicles, the presence or absence of patchy markings, the size of the right and left hepatic lobes, and the degree of splenomegaly. The cumulative survival rate was compared with these peritoneoscopic parameters. The usefulness of peritoneoscopic and histological findings in the prognosis of liver cirrhosis was evaluated using the proportional hazard model of Cox. Significant differences were observed in the cumulative survival rate with respect to the degree of development of regenerating nodules, the size of the right hepatic lobe, the formation of small lymphatic vesicles, and the degree of splenomegaly. Analysis using Cox's proportional hazard model indicated that peritoneoscopic findings are of greater clinical use than histological findings in determining the prognosis of liver cirrhosis.
Adult↗
Cardiopulmonary effects of sevoflurane in cats: comparison with isoflurane, halothane, and enflurane.
The cardiopulmonary effects of sevoflurane (mean, 2.6, 3.8-3.9 and 5.2 per cent) were compared with those of halothane (1.2, 1.8 and 2.4 per cent), enflurane (2.4, 3.6 and 4.8 per cent) and isoflurane (1.6, 2.4 and 3.2-3.3 per cent) at end-tidal concentrations equivalent to 1, 1.5 and 2 minimal alveolar concentrations (MACs) during spontaneous or controlled ventilation (SV or CV) in 57 cats. Cats were assigned to four groups of nine animals each in SV trial and four groups of five or six animals each in CV trial. During SV, respiration rate was decreased by sevoflurane and isoflurane at 2 MAC and by enflurane at each MAC multiple when compared with control values, whereas halothane increased respiration rate at 2 MAC. The degree of hypercapnia and acidosis induced by sevoflurane was not different from that induced by isoflurane and was less than that induced by halothane at 1 to 1.5 MAC or enflurane at 2 MAC. During SV and CV, four anaesthetics decreased heart rate at 2 MAC when compared with control values, but there was no significant difference between anaesthetics. Sevoflurane, like halothane and isoflurane, induced hypotension at 2 MAC when compared with 1 MAC.
Anesthetics, Inhalation↗
Popliteal artery entrapment syndrome: aberrant origin of gastrocnemius muscle shown by 3D CT.
PURPOSE: The purpose of this report is to evaluate the usefulness of 3D CT in the diagnosis of popliteal artery entrapment (PAE) syndrome. METHOD: Three patients (three men, 22-70 years old) with suspected PAE syndrome were examined using helical CT. 3D images were reconstructed to evaluate the relationship between the popliteal artery and the gastrocnemius muscle. 3D arteriograms were simultaneously reconstructed. RESULTS: One patient had unilateral type 3 PAE syndrome. Bilateral PAE syndrome was seen in the other two cases: One had bilateral type 2 anomalies and the other had type 3 on the left and type 2 on the right. In all three cases the anomalous origin of the medial head of the gastrocnemius muscle and its relationship to popliteal artery were clearly visualized by 3D CT. In addition, CT arteriography could detect occlusion, deviation, or stenosis of the popliteal arteries. CONCLUSION: 3D CT is useful in the diagnosis and management of PAE syndrome.
Adult↗
Hyperamylasemia associated with hepatocellular carcinoma.
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Amylases↗
Effects of thiopental, ketamine, diazepam, xylazine, and nitrous oxide on EEG spike activity and convulsive behavior during enflurane anesthesia in atropinized cats. Effect of increasing inhalant concentrations.
The effects of thiopental, ketamine, diazepam, xylazine, and nitrous oxide, and the combinations of thiopental-nitrous oxide and ketamine-nitrous oxide, on both enflurane-induced electroencephalographic (EEG) spike activity and convulsive behavior were measured quantitatively in atropinized cats receiving enflurane with controlled ventilation. Pretreatments with thiopental, ketamine, and diazepam reduced both EEG spike frequency and amplitude at 2.5% to 4.5% inspired enflurane but did not abolish spike activity. Nitrous oxide (66% of inspired gas) did not significantly alter spike frequency or amplitude during 2.5% to 4.5% inspired enflurane, but the combination of thiopental-nitrous oxide or ketamine-nitrous oxide reduced EEG spike activity during 2.5% inspired enflurane. Enflurane-induced convulsive score was markedly suppressed by thiopental and ketamine and was significantly reduced by diazepam, xylazine and nitrous oxide. The combinations of thiopental-nitrous oxide and ketamine-nitrous oxide greatly reduced behavioral-convulsive responses induced by 2.5% to 4.5% inspired enflurane.
Anesthesia, Inhalation↗
Effects of thiopental, ketamine, diazepam, xylazine, and nitrous oxide on EEG spike activity and convulsive behavior during enflurane anesthesia in spontaneously breathing atropinized cats. Effect at surgical depth.
The effects of thiopental, ketamine, diazepam, xylazine and nitrous oxide, and combinations of thiopental-nitrous oxide and ketamine-nitrous oxide on electroencephalographic (EEG) spike activity and convulsive behaviors in atropinized cats at surgical depth of enflurane anesthesia were assessed quantitatively for 60 minutes during spontaneous ventilation. Mean inspired enflurane concentrations (MIEC) were reduced 16% to 29% by pretreatment with thiopental, ketamine, diazepam, and xylazine, and were reduced 19% by 66% nitrous oxide. The MIEC of cats anesthetized with thiopental-nitrous oxide-enflurane and ketamine-nitrous oxide-enflurane were 35% to 38% lower than that with nitrous oxide-enflurane. Pretreatment with thiopental, ketamine, diazepam, and xylazine did not reduce the EEG spike frequency during anesthesia but did markedly reduce the spike amplitude. The addition of 66% nitrous oxide did not alter the spike frequency during anesthesia but tended to reduce the spike amplitude. Combinations of thiopental-nitrous oxide and ketamine-nitrous oxide almost abolished the spike activity. The addition of 66% nitrous oxide prevented convulsive responses elicited by photic and auditory stimulation during enflurane anesthesia. Treatment with thiopental, ketamine, diazepam and xylazine, and combinations of thiopental-nitrous oxide and ketamine-nitrous oxide, completely prevented convulsive responses during enflurane anesthesia.
Anesthesia, Inhalation↗
Comparisons of sevoflurane, isoflurane, and halothane anesthesia in spontaneously breathing cats.
The clinical effects of sevoflurane, isoflurane, and halothane anesthesia with or without nitrous oxide, were compared in healthy, premedicated cats breathing spontaneously during 90 minutes of anesthesia. The effect of nitrous oxide in accelerating the induction of and recovery from anesthesia was more evident for halothane than for sevoflurane or isoflurane. The cats recovered more rapidly from sevoflurane-oxygen than from either halothane- or isoflurane-oxygen. Heart rates did not significantly change during anesthesia with any of the anesthetics. Arterial blood pressures during sevoflurane-oxygen anesthesia were somewhat higher than those with either isoflurane- or halothane-oxygen. There were no significant differences in arterial blood pressures among sevoflurane, isoflurane, and halothane anesthesia when combined with nitrous oxide. The respiration rate during sevoflurane-oxygen was similar to that during halothane-oxygen. There were no significant differences in respiration rate among sevoflurane, isoflurane, and halothane anesthesia when combined with nitrous oxide. The degree of hypercapnia and acidosis during sevoflurane anesthesia was similar to that observed during isoflurane anesthesia and less than during halothane anesthesia. The three anesthetic regimens, with or without nitrous oxide, induced a similar degree of hyperglycemia and hemodilution during anesthesia. Serum biochemical examination did not reveal any hepatic or renal injuries after each anesthesia.
Acid-Base Equilibrium↗
Non-invasive diagnosis of ischemic brain damage after cardiopulmonary resuscitation in dogs by using transcranial Doppler ultrasonography.
We have attempted to identify whether it is possible to utilize transcranial Doppler ultrasonography to evaluate the brain damage that occurs after resuscitation from 3 min (control group) and 12 min (damage group) of cardiac arrest in dogs. In this study we used transcranial Doppler ultrasonography to follow the basilar arterial flow and middle cerebral arterial flow for 180 min following the induction of cardiac arrests. Two abnormal waveform patterns (the "to-and-fro" and "diastolic no-flow" patterns) were found in all dogs in the damage group whereas abnormal waveforms were not detected in the control group. Pathological diagnosis revealed that, compared with the control group, the damage group recognized ischemic alteration at the level of the hippocampus and caudate nucleus. In conclusion, this study shows that the basilar arterial flow of observed with transcranial Doppler ultrasonography may be use for the prediction of outcome and the diagnosis of brain damage in the dog.
Animals↗
Peripheral hypoglossal nerve palsy caused by lateral position of the external carotid artery and an abnormally high position of bifurcation of the external and internal carotid arteries--a case report.
An unusual case of peripheral hypoglossal nerve palsy, caused by lateral position of the external carotid artery and an abnormally high carotid bifurcation is reported. Improvement followed ligation and cutting of the external carotid artery at its origin.
Carotid Artery, External↗
Natural course of cavernous hepatic hemangioma.
Cavernous hepatic hemangiomas are benign liver tumors and present as incidental findings on sonographic examinations, but little is known concerning their natural course. Therefore, we performed a clinical and imaging follow-up of 64 cases of cavernous hepatic hemangioma in 50 patients during an average 18.8 month period. One case presented a symptom of slightly right upper quadrant pain and two cases showed thrombocytopenia. In one of the thrombocytopenia cases, cavernous hepatic hemangioma was resected because of Kasabach-Merrit syndrome. No case increased in size during follow-up, but one case decreased and disappeared. These results suggested that prolonged clinical and imaging follow-up of cavernous hepatic hemangiomas may be needed.
Adult↗
Disappearance of gastric antral vascular ectasia after percutaneous transhepatic obliteration.
We report on a patient with severe iron deficiency anemia due to gastric antral vascular ectasia (GAVE), complicating hepatocellular carcinoma (HCC) and esophageal varices. Esophago-gastro-duodenoscopy (EGDS) 6 months after transarterial embolization (TAE) for the HCC and percutaneous transhepatic obliteration (PTO) for esophageal varices, showed the absence of GAVE. As GAVE did not recur in spite of the recurrence of the tumor thrombus later, lowered antral congestion by PTO might be the main cause of disappearance of GAVE. This case suggests that PTO may be an effective treatment against GAVE with portal hypertension with uncontrollable bleeding.
Aged↗
Reduced expression of ICE/caspase1 and CPP32/caspase3 in human hepatocellular carcinoma.
The interleukin-1(-converting enzymes (ICE)/caspase1 and the CPP32/caspase3, cysteine proteases, play an important role in the maintenance of homeostasis by inducing apoptosis. Since human hepatocellular carcinomas (HCCs) demonstrate strong resistance to apoptosis, we investigated the expression of ICE and CPP32 in human HCCs. Reverse transcription PCR analysis revealed that one out of five HCC tissues showed no band of ICE mRNA and two out of five HCC tissues showed no band of CPP32 mRNA. An immunohistochemical study of 20 cases of HCC tissues and non-tumor parts revealed that immunoreactivity of ICE and CPP32 was preferentially observed in the cytoplasm, appearing as a diffuse and homogeneous pattern. Some nuclei also stained with anti-ICE antibody or anti-CPP32 antibody and demonstrated apoptotic features. Overall, the expression of ICE and CPP32 were significantly down-regulated in the HCCs compared to nontumor cells. In situ nick end labeling method (TUNEL) labeling index significantly decreased according to the decreasing staining intensity of CPP32. However, there was no tendency for the TUNEL labeling index to decrease with decreasing ICE staining intensity. Our results suggested that the expression of ICE and CPP32 were down-regulated and that especially reduced expression of CPP32 may contribute to resistance against apoptosis in human HCCs.
Apoptosis↗
Characterization of a lentogenic Newcastle disease virus isolated from broiler chickens in Japan.
Newcastle disease virus (NDV), named MET95, was isolated from a non-vaccinated broiler flock in Japan in 1995. The MET95 strain was determined to be a lentogenic NDV. The strain has the properties of eluting rapidly at 4 C and has low thermostability in hemagglutinating activity with chicken erythrocytes. In these studies, no difference could be found between the MET95 strain and the Hitcher B1 vaccine strain. However, the chickens inoculated with the MET95 strain, as well as chickens that they were in contact with, had a much higher hemagglutination-inhibition antibody response than those inoculated with the B1 strain. Accordingly, the MET95 strain is thought to be a promising candidate as a live ND vaccine strain. In Japan, this is the first report on the isolation of lentogenic NDV from chickens since the paper on the Ishii strain isolated in 1966.
Animals↗
Histological features of cirrhosis with hepatitis C virus for prediction of hepatocellular carcinoma development; a prospective study.
The histological features of pre-neoplastic lesions in HCV-associated cirrhosis remain uncertain. The aim of this prospective study was to elucidate histological features for predicting the development of hepatocellular carcinoma (HCC). A cohort of 72 consecutive patients with hepatitis C-associated cirrhosis, which was diagnosed by histology investigated for development of HCC. Seven histological features including small cell dysplasia (SCD) and large cell dysplasia (LCD) of liver cirrhosis were evaluated with regard to the development of HCC. In addition, proliferation and apoptosis were investigated using immunohistochemistry by proliferating cell nuclear antigen and TUNEL method, respectively. At enrollment, SCD was observed in the biopsy specimens of 18 out of 72 (25.0%) patients and LCD was observed in 20 out of 72 (27.8%). Twenty eight out of 72 patients (38.9%) developed HCC during a mean follow-up period of 72.4 months. Among the histological parameters, SCD, active inflammation and complete nodule were statistically significant factors for the cumulative probability of developing HCC. However, LCD did not appear to be important for HCC development. In multivariate analysis, SCD was the highest independent risk factor for HCC. Samples with SCD demonstrated a higher proliferative rate and a lower apoptotic rate than normal hepatocytes or samples with LCD. These results indicate that SCD is a major risk factor for HCC. Careful assessment of liver histology may be important in order to predict HCC development in patients with HCV-related cirrhosis.
Adult↗