Search PubMed⌕ Search

Biomedical subjects

K T Wang

Publications and source records attributed to K T Wang.

At least 73 records · Page 4Linked to original sources
PubMed1979

Enzymic synthesis of opioid peptides.

Protected opioid pentapeptides were synthesized by the papain-catalyzed condensation of small synthetic fragments. These peptides, free as well as protected, were identical with those prepared by the dicyclohexylcarbodiimide-1-hydroxybenzotriazole method.

Dicyclohexylcarbodiimide↗
PubMed1978

Synthesis of a fully active snake venom cardiotoxin by fragment condensation on solid polymer.

A polypeptide cardiotoxin containing 60 amino acids with 4 disulfide bonds has been synthesized by the "fragment solid-phase" method. The identity of the synthetic product with native cardiotoxin was established by chromatography on Sephadex G-50, carboxymethyl-cellulose column chromatography, thin layer chromatography, disc gel electrophoresis, amino acid analysis, end group analysis, peptide mapping, circular dichroism spectra, and four biological tests.

Amino Acid Sequence↗
PubMed1978

Purfication of synthetic cardiotoxin by affinity chromatography.

A polypeptide containing 60 amino acids with 4 disulphide bonds, synthesized by the solid-phase method, was highly purifed by anticardiotoxin-Sepharose affinity chromatography following gel filtration and CM-cellulose chromatography. The identification of the final product as cardiotoxin was confirmed by thin-layer chromatography on silica gel, polyacrylamide gel electrophoresis, amino acid analysis, circular dichroism spectra, N-terminal analysis and four biological tests.

Animals↗
PubMed1978

Renaturation of a reduced Taiwan cobra cardiotoxin.

Refolding of a denatured protein obtained by reducing cardiotoxin from the Taiwan cobra with mercaptoethanol has been carried out in aqueous and non-aqueous solutions. Oxidation of the reduced protein in 0.05 M phosphate buffer (pH 7.2) resulted in isolating an active protein which showed, as compared to native cardiotoxin, identical conformation and biological activities such as lethality, antigenicity and muscle contracture inducing activity. On the other hand, the reduced protein was undergoing incorrect SS-pairing and several inactive products were formed in a mixture of 1,2-ethanediol and 1-propanol (1 : 1; v/v).

Animals↗